• Title/Summary/Keyword: crops

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Constructing Reference Transcriptome Sets of Codonopsis lanceolate(Deodeok) and Ixeridium dentatum

  • Tae-Ho Lee;Yun-Ho Oh
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.242-242
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    • 2022
  • As the aging population increases and interest in well-being increases, the importance of developing special crops increases. Natural medicine based on the special crops has been mainly used as an adjunct therapy for many diseases and symptoms based on culture, traditional medicine, and experience. In particular, it is attracting attention as a new resource to develop new drugs such as Artemisinin, a treatment for malaria. In order to efficiently use crops, it is essential to establish omics data such as genomes, transcriptomes, and metabolites of special-purpose crops. However, many special-purpose crops have large, heterogeneous and polyploid genomes that require high cost and long time to reference genome sequencing. Therefore, we built an inexpensive, fast, but very usefill reference transcriptome as the first step. We constructed high-quality reference transcriptom sets of Codonopsis lanceolata and Ixeridium dentatum with PacBio data. Our team will continue to construct reference transcriptoms of more special-purpose crops, and the data will be released by the National Agricultural Biotechnology Information Center (NABIC) in order to be widely used in agricultural as well as medical R&D.

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Reliability and Validity of the Korean Version of the Child Report of Post-Traumatic Symptoms (CROPS) and the Parent Report of Post-Traumatic Symptoms (PROPS) (한국판 아동용 외상 후 증상 보고와 부모용 외상 후 증상 보고에 대한 신뢰도 및 타당도 연구)

  • Lee, Kwang-Min;Jeong, Sung-Hoon;Lee, Won-Kee;Chung, Un-Sun
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.22 no.3
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    • pp.169-181
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    • 2011
  • Objectives: The Child Report of Post-traumatic Symptoms (CROPS) and the Parent Report of Post-traumatic Symptoms (PROPS) are screening measures for post-traumatic symptoms in children. The present study aimed to investigate the reliability and validity of the Korean versions of the CROPS and the PROPS. Methods: The Korean versions of the CROPS and the PROPS were administered to a sample of 304 children aged 6 to 15 years old. The internal reliability, test-retest reliability, factorial validity, predictive validity and concurrent validity were evaluated. Results: The reliability of the CROPS and the PROPS was shown to have excellent internal consistency and test-retest correlation. The single factor structure of the PROPS was good and that of the CROPS was borderline acceptable according to confirmatory factor analysis. Other validity measures such as the predictive validity and concurrent validity were also shown to be satisfactory. Conclusion: This study demonstrated that the Korean versions of the CROPS and the PROPS were reliable measures with satisfactory psychometric qualities. Because it takes less than 5 minutes to fill out the CROPS and the PROPS, respectively they can be quick and easy screening scales for assessing post-traumatic symptoms in Korean children.

Scientific considerations for the biosafety of the off-target effects of gene editing in crops (유전자교정작물 내 비의도적 돌연변이의 안전성 논란에 관한 과학적 고찰)

  • Lee, Shin-Woo;Kim, Yun-Hee
    • Journal of Plant Biotechnology
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    • v.47 no.3
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    • pp.185-193
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    • 2020
  • The number of commercially approved gene-edited crops is gradually increasing, and in South Korea, it has led to intense investment in gene-edited crop development to increase international competitiveness. However, as with genetically modified crops, the safety of gene-edited crops regarding unexpected risks for humans and the environment is subject to an ongoing debate. In particular, unintentional "off-target effects" have become the center of controversy. In this review, we discuss typical plant characteristics (including somatic variation and ploidy), the extent of various off-target effects in genetically modified crops generated via horizontal transfer in nature, and the off-target effects in commercial genetically modified crops. We conclude that most off-target effects possibly occurring in gene-edited crops are not expected to be critically harmful to humans or the environment. Therefore, existing regulation for genetically modified crops should be enough for the risk assessment of gene-edited crops.

Incorporation Effect of Green Manure Crops on Improvement of Soil Environment on Saemangeum Reclaimed Land during Silage Corn Cultivation

  • Yang, Chang-Hyu;Lee, Jang-Hee;Baek, Nan-Hyun;Shin, Pyeong;Cho, Kwang-Min;Lee, Sang-Bok;Lee, Gyeong-Bo
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.3
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    • pp.187-192
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    • 2013
  • This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during silage corn cultivation over the past two years. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by addition rate of nitrogen fertilizer (100 kg $ha^{-1}$) with 30 - 100% of non nitrogen fertilization (NNF). Soil physico-chemical properties and growth and yield potential of silage corn were examined. The tested soils showed strong alkali and saline properties with low contents of organic matter and available phosphate while contents of exchangeable sodium and magnesium were high. Soil salinity increased during cultivation of summer crop. However, corn was not affected by salt content. The fresh weight of GMC at incorporation time was 18,345 kg $ha^{-1}$. Content of total nitrogen was 3.09% and the C/N ratio was 12.8 at incorporation time. Fresh and dry matter yield of silage corn were higher in the order of N30% reduction, CF, N50% reduction, N70% reduction, N100% reduction and NNF. Fresh and dry matter yield potential of silage corn for N30% reduction were comparable to those of CF. Bulk density of the soil decreased with incorporation of GMC, while porosity was increased. The soil pH decreased while content of exchangeable calcium, available phosphate, and organic matter increased. Also contents of exchangeable sodium and potassium decreased with incorporation of GMC. The data indicate that incorporation of hairy vetch can improve soil physical and chemical properties and reduce nitrogen fertilizer application especially for alkali saline reclaimed soil such as Saemangeum reclaimed land.

Political Implication on the Genetically Modified Crops (유전자(遺傳子) 변형(變形) 농산물(農産物)에 관한 정책적(政策的) 함의(含意))

  • Shin, Young In;Park, In Shik
    • Korean Journal of Agricultural Science
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    • v.26 no.2
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    • pp.116-129
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    • 1999
  • The objectives of the study mainly concentrated on identifying the political implication on genetically modified crops considering production potentials and problems. It is found that the comprehensive evaluation of the genetically modified crops could not be concluded and compromised in a word on account of the polarization and parallelism of the positive and negative benefits of genetically modified crops just like as the rail way. The genetically modified crops will be contributed to solving the food shortage problems in the world, when the issues such as food safety, ecological disturbance and loss and degradation of biodiversity can be guaranteed in transparency. And when the trade morality of the multi-national enterprises be accepted by the genetically modified crops consumers, the potentiality of genetically modified crops will be realized greatly. By the way, the first problems will be expected to be solved by scientific development. If the food safety of the genetically modified technology be verified in transparency, it will be greatly contributed to solve food problems of human beings in the world. But the second problem could not be expected to be easily solved from the view point of capital property. In conclusion, the genetically modified technology will be made a severe sense of incongruity and a seed of fire on it will be remained persistently. According to the results based on the analysing the genetically modified crops potentiality and problems, it was identified that the ex-ante preparation of counter-measures and actions on it should be necessary. Accordingly this study has recommended that how and what the R&D policy on genetically modified crops be established and suggested how to carried out the industrial and economic policy together with international negotiation, and organizational and institutional rearrangement and etc.

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Characteristics of Phytoplankton Communities in the Coastal Waters of Power Plant (발전소 주변해역 식물플랑크톤의 군집 특성)

  • Kang, Yeon-Shik
    • ALGAE
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    • v.23 no.1
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    • pp.31-52
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    • 2008
  • This study analyzes characteristics of phytoplankton communities around Wolseong nuclear power plant by selecting 16 stations from July 2006 to June 2007 and understands the influences on standing crops and chlorophyll a of phytoplankton by passing through the cooling water system. The total species number is 283, among which diatoms is 208 occupying 73.5% of total taxa. The mean of total standing crops is 469,380-3,704,114 cells L-1. It is the highest in April 2007 because blooming of Chaetoceros socialis occurs during this period. The mean standing crops of microplankton and nanoplankton are average 129,666-3,392,640 cells L-1 and 240,943-650,505 cells L-1 respectively, which occupy 54.01% and 46.54% of total standing crops. The mean concentrations of total chlorophyll a is 0.64-5.39 μg L-1. The mean concentrations of chlorophyll a of microplankton, nanoplankton and picoplankton are 1.33 μg L-1, 0.21 μg L-1 and 0.49 μg L-1 respectively. Dominant species around Wolseong neclear power plant during this study are Chaetoceros debilis, Chaetoceros socialis, Leptocylindrus danicus, Pseudo-nitzschia fraudulenta, P. subfraudulenta and Thalassiosira decipiens. Fluctuation rates of standing crops and chlorophyll a concentrations of phytoplankton passing through the cooling water system are 22.80% and 50.48% respectively. Decrease of standing crops and chlorophyll a concentrations of phytoplankton means that community structure of phytoplnakton may change at the discharge areas.

Identification and Heterologous Expression of a ${\Delta}4$-Fatty Acid Desaturase Gene from Isochrysis sphaerica

  • Guo, Bing;Jiang, Mulan;Wan, Xia;Gong, Yangmin;Liang, Zhuo;Hu, Chuanjiong
    • Journal of Microbiology and Biotechnology
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    • v.23 no.10
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    • pp.1413-1421
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    • 2013
  • The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, $C20:5{\omega}-3$) and docosahexaenoic acid (DHA, $C22:6{\omega}-3$) that are important to human health. Here, we report a functional characterization of a ${\Delta}4$-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.

A survey of the genetic components introduced into approved GM crops (국내외 상업화 GM 작물의 유전요소 분석)

  • Woo, Hee-Jong;Chung, Chan-Mi;Shin, Kong-Sik;Ji, Hyeon-So;Lee, Ki-Jong;Suh, Seok-Chul;Kweon, Soon-Jong;Cho, Yong-Gu
    • Journal of Plant Biotechnology
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    • v.36 no.2
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    • pp.106-114
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    • 2009
  • Genetic components introduced into approved GM crops are a key subject for safety assessment and provide a basis for the development of detection methods for GM crops. In order to understand the genetic components in approved GM crops comprehensively, we screened the genetic vector maps of GM crops that had been approved for commercialization around the world. A total of 64 varieties from 5 major GM crop species (maize, canola, cotton, soybean, and tomato) were subjected to analysis. The genetic components included genes, promoters, terminators, and selection marker. This survey may be useful for researchers who develop GM crops and methods for detecting GM crops.

Variations in Root and Tuber Crops Production due to Climate Change

  • Hwang, Sung-Eun;Chon, Chun-Hwang;Park, Geon-Young
    • Journal of Integrative Natural Science
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    • v.8 no.2
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    • pp.135-140
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    • 2015
  • Climate change which occuring the recent abrupt fluctuations in meteorological and climatological elements is bound, brings about more significant impacts and changes in human life One of the most important problems due to the impacts of climate change tends to have been decreased the food production, which is expected to make crop resources more and more important. Accordingly, agricultural meteorology should also become more important. In this study, the correlation between meteorological elements and root and tuber crops (potatoes and sweet potatoes), which are emergency crops, and meteorological elements were analyzed, and the impacts of climate changes on the production of such crops were examined. This study concludes that agriculture and food resources are important, and suggests that we should prepare for changes in crops, the weaponization of food, and the lack of water resources in the future. The meteorological element and crops element correlation analysis results. Sweet potatoes, which are negatively influenced by climate change, need breeding improvement and cultivation method development, and potatoes, which are positively influenced by climate change, require preparations for climate changes that exceed the climatic limit. The variations of agricultural production contributed to changes in crop production. Therefore, the importance of agricultural meteorology and the food crop industry should be fully recognized to prepare for climate change.