• Title/Summary/Keyword: crops

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Constructing Reference Transcriptome Sets of Codonopsis lanceolate(Deodeok) and Ixeridium dentatum

  • Tae-Ho Lee;Yun-Ho Oh
    • 한국작물학회:학술대회논문집
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    • 한국작물학회 2022년도 추계학술대회
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    • pp.242-242
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    • 2022
  • As the aging population increases and interest in well-being increases, the importance of developing special crops increases. Natural medicine based on the special crops has been mainly used as an adjunct therapy for many diseases and symptoms based on culture, traditional medicine, and experience. In particular, it is attracting attention as a new resource to develop new drugs such as Artemisinin, a treatment for malaria. In order to efficiently use crops, it is essential to establish omics data such as genomes, transcriptomes, and metabolites of special-purpose crops. However, many special-purpose crops have large, heterogeneous and polyploid genomes that require high cost and long time to reference genome sequencing. Therefore, we built an inexpensive, fast, but very usefill reference transcriptome as the first step. We constructed high-quality reference transcriptom sets of Codonopsis lanceolata and Ixeridium dentatum with PacBio data. Our team will continue to construct reference transcriptoms of more special-purpose crops, and the data will be released by the National Agricultural Biotechnology Information Center (NABIC) in order to be widely used in agricultural as well as medical R&D.

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한국판 아동용 외상 후 증상 보고와 부모용 외상 후 증상 보고에 대한 신뢰도 및 타당도 연구 (Reliability and Validity of the Korean Version of the Child Report of Post-Traumatic Symptoms (CROPS) and the Parent Report of Post-Traumatic Symptoms (PROPS))

  • 이광민;정성훈;이원기;정운선
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제22권3호
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    • pp.169-181
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    • 2011
  • Objectives: The Child Report of Post-traumatic Symptoms (CROPS) and the Parent Report of Post-traumatic Symptoms (PROPS) are screening measures for post-traumatic symptoms in children. The present study aimed to investigate the reliability and validity of the Korean versions of the CROPS and the PROPS. Methods: The Korean versions of the CROPS and the PROPS were administered to a sample of 304 children aged 6 to 15 years old. The internal reliability, test-retest reliability, factorial validity, predictive validity and concurrent validity were evaluated. Results: The reliability of the CROPS and the PROPS was shown to have excellent internal consistency and test-retest correlation. The single factor structure of the PROPS was good and that of the CROPS was borderline acceptable according to confirmatory factor analysis. Other validity measures such as the predictive validity and concurrent validity were also shown to be satisfactory. Conclusion: This study demonstrated that the Korean versions of the CROPS and the PROPS were reliable measures with satisfactory psychometric qualities. Because it takes less than 5 minutes to fill out the CROPS and the PROPS, respectively they can be quick and easy screening scales for assessing post-traumatic symptoms in Korean children.

유전자교정작물 내 비의도적 돌연변이의 안전성 논란에 관한 과학적 고찰 (Scientific considerations for the biosafety of the off-target effects of gene editing in crops)

  • 이신우;김윤희
    • Journal of Plant Biotechnology
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    • 제47권3호
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    • pp.185-193
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    • 2020
  • 최근, 유전자교정 작물의 상업화 승인 건수가 급속하게 늘어나고 있으며, 국내에서도 유전자교정 작물의 개발에 대한 집중적인 투자를 통하여 국제경쟁력을 높이기 위하여 노력하고 있다. 그러나 기존의 유전자변형작물의 상업화 과정에서 끊임없이 제기되어온 인체 및 환경에 대한 잠재적인 위해성 논란이 유전자교정 작물에 대하여서도 제기되고 있다. 특히, 비의도적 돌연변이(off-target)가 가장 큰 논란의 중심이 되고 있다. 따라서 본 리뷰는 식물이 내포하고 있는 장점인 배수체, 체세포 돌연변이 그리고 자연 상태에서 아그로박테리아의 T-DNA 단편의 수평 전이로 창출된 자연적인 유전자변형작물과 기존에 상업화가 승인된 유전자변형작물 이벤트들의 게놈 내 비의도적 돌연변이 사례 등을 검토한 결과 유전자교정 작물에서 나타나는 대부분의 비의도적 돌연변이는 인체 및 환경에 미칠 수 있는 위해성을 우려할 만한 수준이 아니라고 할 수 있었다. 이에, 유전자교정 작물의 안전성 평가를 위하여 새로운 규정을 제정할 필요가 없으며 기존의 유전자변형작물의 안전 관리규정을 일부 "용어의 정의" 등만 개정하여 적용하면 충분할 것으로 사료 되었다.

Incorporation Effect of Green Manure Crops on Improvement of Soil Environment on Saemangeum Reclaimed Land during Silage Corn Cultivation

  • Yang, Chang-Hyu;Lee, Jang-Hee;Baek, Nan-Hyun;Shin, Pyeong;Cho, Kwang-Min;Lee, Sang-Bok;Lee, Gyeong-Bo
    • 한국토양비료학회지
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    • 제46권3호
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    • pp.187-192
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    • 2013
  • This study was carried out to investigate the incorporation effect of green manure crops (GMC) such as the hairy vetch on improvement of soil environment in reclaimed land during silage corn cultivation over the past two years. Plots consisted of conventional fertilization (CF) and incorporation of GMC were divided by addition rate of nitrogen fertilizer (100 kg $ha^{-1}$) with 30 - 100% of non nitrogen fertilization (NNF). Soil physico-chemical properties and growth and yield potential of silage corn were examined. The tested soils showed strong alkali and saline properties with low contents of organic matter and available phosphate while contents of exchangeable sodium and magnesium were high. Soil salinity increased during cultivation of summer crop. However, corn was not affected by salt content. The fresh weight of GMC at incorporation time was 18,345 kg $ha^{-1}$. Content of total nitrogen was 3.09% and the C/N ratio was 12.8 at incorporation time. Fresh and dry matter yield of silage corn were higher in the order of N30% reduction, CF, N50% reduction, N70% reduction, N100% reduction and NNF. Fresh and dry matter yield potential of silage corn for N30% reduction were comparable to those of CF. Bulk density of the soil decreased with incorporation of GMC, while porosity was increased. The soil pH decreased while content of exchangeable calcium, available phosphate, and organic matter increased. Also contents of exchangeable sodium and potassium decreased with incorporation of GMC. The data indicate that incorporation of hairy vetch can improve soil physical and chemical properties and reduce nitrogen fertilizer application especially for alkali saline reclaimed soil such as Saemangeum reclaimed land.

유전자(遺傳子) 변형(變形) 농산물(農産物)에 관한 정책적(政策的) 함의(含意) (Political Implication on the Genetically Modified Crops)

  • 신용인;박인식
    • 농업과학연구
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    • 제26권2호
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    • pp.116-129
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    • 1999
  • The objectives of the study mainly concentrated on identifying the political implication on genetically modified crops considering production potentials and problems. It is found that the comprehensive evaluation of the genetically modified crops could not be concluded and compromised in a word on account of the polarization and parallelism of the positive and negative benefits of genetically modified crops just like as the rail way. The genetically modified crops will be contributed to solving the food shortage problems in the world, when the issues such as food safety, ecological disturbance and loss and degradation of biodiversity can be guaranteed in transparency. And when the trade morality of the multi-national enterprises be accepted by the genetically modified crops consumers, the potentiality of genetically modified crops will be realized greatly. By the way, the first problems will be expected to be solved by scientific development. If the food safety of the genetically modified technology be verified in transparency, it will be greatly contributed to solve food problems of human beings in the world. But the second problem could not be expected to be easily solved from the view point of capital property. In conclusion, the genetically modified technology will be made a severe sense of incongruity and a seed of fire on it will be remained persistently. According to the results based on the analysing the genetically modified crops potentiality and problems, it was identified that the ex-ante preparation of counter-measures and actions on it should be necessary. Accordingly this study has recommended that how and what the R&D policy on genetically modified crops be established and suggested how to carried out the industrial and economic policy together with international negotiation, and organizational and institutional rearrangement and etc.

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발전소 주변해역 식물플랑크톤의 군집 특성 (Characteristics of Phytoplankton Communities in the Coastal Waters of Power Plant)

  • 강연식
    • ALGAE
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    • 제23권1호
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    • pp.31-52
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    • 2008
  • This study analyzes characteristics of phytoplankton communities around Wolseong nuclear power plant by selecting 16 stations from July 2006 to June 2007 and understands the influences on standing crops and chlorophyll a of phytoplankton by passing through the cooling water system. The total species number is 283, among which diatoms is 208 occupying 73.5% of total taxa. The mean of total standing crops is 469,380-3,704,114 cells L-1. It is the highest in April 2007 because blooming of Chaetoceros socialis occurs during this period. The mean standing crops of microplankton and nanoplankton are average 129,666-3,392,640 cells L-1 and 240,943-650,505 cells L-1 respectively, which occupy 54.01% and 46.54% of total standing crops. The mean concentrations of total chlorophyll a is 0.64-5.39 μg L-1. The mean concentrations of chlorophyll a of microplankton, nanoplankton and picoplankton are 1.33 μg L-1, 0.21 μg L-1 and 0.49 μg L-1 respectively. Dominant species around Wolseong neclear power plant during this study are Chaetoceros debilis, Chaetoceros socialis, Leptocylindrus danicus, Pseudo-nitzschia fraudulenta, P. subfraudulenta and Thalassiosira decipiens. Fluctuation rates of standing crops and chlorophyll a concentrations of phytoplankton passing through the cooling water system are 22.80% and 50.48% respectively. Decrease of standing crops and chlorophyll a concentrations of phytoplankton means that community structure of phytoplnakton may change at the discharge areas.

Identification and Heterologous Expression of a ${\Delta}4$-Fatty Acid Desaturase Gene from Isochrysis sphaerica

  • Guo, Bing;Jiang, Mulan;Wan, Xia;Gong, Yangmin;Liang, Zhuo;Hu, Chuanjiong
    • Journal of Microbiology and Biotechnology
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    • 제23권10호
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    • pp.1413-1421
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    • 2013
  • The marine microalga Isochrysis sphaerica is rich in the very-long-chain polyunsaturated fatty acids, including eicosapentaenoic acid (EPA, $C20:5{\omega}-3$) and docosahexaenoic acid (DHA, $C22:6{\omega}-3$) that are important to human health. Here, we report a functional characterization of a ${\Delta}4$-fatty acid desaturase gene (FAD4) from I. sphaerica. IsFAD4 contains a 1,284 bp open reading frame encoding a 427 amino acid polypeptide. The deduced amino sequence comprises three conserved histidine motifs and a cytochrome b5 domain at its N-terminus. Phylogenetic analysis indicated that IsFad4 formed a unique Isochrysis clade distinct from the counterparts of other eukaryotes. Heterologous expression of IsFAD4 in Pichia pastoris showed that IsFad4 was able to desaturate docosapentaenoic acid (DPA) to form DHA, and the rate of converting DPA to DHA was 79.8%. These results throw light on the potential industrial production of specific polyunsaturated fatty acids through IsFAD4 transgenic yeast or oil crops.

국내외 상업화 GM 작물의 유전요소 분석 (A survey of the genetic components introduced into approved GM crops)

  • 우희종;정찬미;신공식;지현소;이기종;서석철;권순종;조용구
    • Journal of Plant Biotechnology
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    • 제36권2호
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    • pp.106-114
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    • 2009
  • Genetic components introduced into approved GM crops are a key subject for safety assessment and provide a basis for the development of detection methods for GM crops. In order to understand the genetic components in approved GM crops comprehensively, we screened the genetic vector maps of GM crops that had been approved for commercialization around the world. A total of 64 varieties from 5 major GM crop species (maize, canola, cotton, soybean, and tomato) were subjected to analysis. The genetic components included genes, promoters, terminators, and selection marker. This survey may be useful for researchers who develop GM crops and methods for detecting GM crops.

Variations in Root and Tuber Crops Production due to Climate Change

  • Hwang, Sung-Eun;Chon, Chun-Hwang;Park, Geon-Young
    • 통합자연과학논문집
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    • 제8권2호
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    • pp.135-140
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    • 2015
  • Climate change which occuring the recent abrupt fluctuations in meteorological and climatological elements is bound, brings about more significant impacts and changes in human life One of the most important problems due to the impacts of climate change tends to have been decreased the food production, which is expected to make crop resources more and more important. Accordingly, agricultural meteorology should also become more important. In this study, the correlation between meteorological elements and root and tuber crops (potatoes and sweet potatoes), which are emergency crops, and meteorological elements were analyzed, and the impacts of climate changes on the production of such crops were examined. This study concludes that agriculture and food resources are important, and suggests that we should prepare for changes in crops, the weaponization of food, and the lack of water resources in the future. The meteorological element and crops element correlation analysis results. Sweet potatoes, which are negatively influenced by climate change, need breeding improvement and cultivation method development, and potatoes, which are positively influenced by climate change, require preparations for climate changes that exceed the climatic limit. The variations of agricultural production contributed to changes in crop production. Therefore, the importance of agricultural meteorology and the food crop industry should be fully recognized to prepare for climate change.