• Title/Summary/Keyword: cp rbcL and matK genes

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A new distribution record of Chrysosplenium grayanum Maxim. (Saxifragaceae) in Korea: Evidence from morphological and molecular data

  • Choi, Ji-Eun;In, Kyung-Ho;Kim, Bong Seok;Kim, Kyeonghee;Kim, Jin-Seok;Kim, Yong-In;Lee, Byoung Yoon;Lim, Chae Eun
    • Journal of Species Research
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    • v.9 no.1
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    • pp.46-55
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    • 2020
  • Chrysosplenium grayanum Maxim. (Series Nepalensia), which had been known to be restricted to Japan, was newly discovered from Mt. Cheongtae in Yeonggwang-gun, Jeollanam-do, located in the southern part of the Korean Peninsula. Species identification was confirmed using morphological characteristics and DNA sequence data, while comparing with materials obtained from Japan and herbarium specimens. Chrysosplenium grayanum is clearly distinguished from the remaining taxa of the genus Chrysosplenium by having glabrous plant body, opposite leaves, cylindrical papillae with roundish head at the tip on the smooth seed surface, and four stamens. Molecular sequence data of the nuclear ribosomal ITS regions, chloroplast rbcL and matK genes strongly supported that this previously unknown Chrysosplenium species from Korea is C. grayanum. Taking the molecular and the morphological evidence into consideration, it is clear that newly discovered Chrysosplenium population in Korea is conspecific with the widely distributed C. grayanum in Japan. In this paper, we provide a description, illustration, and photo images of Chrysosplenium grayanum from Korea and also a key to the Chrysosplenium species in Korea.

Complete Genome Sequences of Crepidiastrum denticulatum (Asteraceae)

  • Jung, Joonhyung;Hyun, Jongyoung;Do, Hoang Dang Khoa;Kim, Joo-Hwan
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.37-37
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    • 2018
  • The genus Crepidiastrum (Asteraceae), containing ca. 20 species, is mainly distributed in Asia. Crepidiastrum denticulatum, an edible plant that commonly call "e-go-deulppae-gi" in Korean, distributes in Korea, Japan, and China. The complete chloroplast (cp) genome sequences of C. denticulatum was characterized from MiSeq2000 (Illumina Co.) pair-end sequencing data. The cp genome of C. denticulatum has a total sequence length of 152,689 bp and show a typical quadripartite structure. It consists of the large single copy (LSC: 84,022 bp), small single copy (SSC: 18,519 bp), separated by a pair of inverted repeats (IRs: 25,074 bp) and contains 110 unique genes and 18 genes duplicated in the IR regions. Our comparative analysis identified three cpDNA regions (matK, rbcL, and psbA-trnH) from three Crepidiastrum species, which may be useful for molecular identification of each species, and providing a guideline for its clear confirming about dried medical herb.

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