• Korean Journal of Veterinary Research
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• v.53 no.1
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• pp.19-23
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• 2013

This study was carried out to evaluate the diagnosis of acute clinical mastitis (ACM) which was based on the vital signs and complete blood count (CBC) tests in dairy cows. Twenty eight dairy cows diagnosed with ACM, were selected for the study between Jan 2003 and July 2006 in the National Institute of Animal Science. Based on their vital signs (rectal temperature, depression, rumen contraction and, dehydration status), ACM was divided into three different classes; mild, moderate and severe forms. In addition, ACM cows were subjected to CBC tests for further diagnosis of ACM. Of the 27 dairy cows diagnosed with ACM, 3 cows were determined to have a mild form, while moderate and sever forms were each observed in twelve cows. Among of them, 4 cows died, 5 cows were culled and 18 cows were recovered. In the mild form, all haematological parameters were comparable with normal values. However, leukopenia, due to neutropenia and lymphocytopenia, appeared characteristically in the moderate and severe forms. Using the observation of vital signs in conjunction with CBC tests, the diagnosis of ACM is more accurate, and is helpful in making decisions of whether treatment or culling of dairy cows infected with ACM is most appropriate.

• Journal of Animal Reproduction and Biotechnology
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• v.37 no.4
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• pp.266-275
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• 2022

The impact of heat stress (HS) on reproductive performance and its problems in cows remains to be investigated in Bangladesh. The study was, therefore, aimed to evaluate the effect of HS on fertility and reproductive health problems of dairy cows in a selected area of Bangladesh. A total of 1,095 cows from 500 farms were included in this study. The climate-related data were recorded daily basis for every month in a year and temperature-humidity index (THI) values were calculated to determine the level of HS. Concurrently, data on fertility parameters [number of services per conception (NSC), conception rate (CR) and calving to the first service interval (CFSI)], and reproductive problems were collected through a pre-defined questionnaire. The results show that crossbred [Native x Holstein-Friesian, (HF)] cows were more vulnerable (p < 0.05) to a magnitude of HS effects considering physiological parameters of cows [age, body condition score (BCS), parity and milk yield]. Regarding fertility, HS had no effect on NSC, CR and CFSI in both native and crossbred cows (p > 0.05). The findings on the reproductive problems indicate that HS had significant influence on the prevalence of anestrus (χ² = 21.814, p < 0.05) and retained placenta (χ² = 24.632, p < 0.05) in cows. Of note, the prevalence of repeat breeding syndrome was 2.5 folds higher in stress condition than in no stress condition. Abortion and dystocia were not influenced by HS. In conclusion, HS does not influence the fertility parameters of cows studied; however, anestrus and retained placenta are likely to occur under HS conditions in cows.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.3
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• pp.263-265
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• 1995

Blood plasma minerals and their effects towards the fertility in 136 cows randomly selected from 26 dairy farms in central Thailand were studied. An average of 8.60 mg% Ca, 5.97 mg% P, 2.45 mg% Mg, $85.9{\mu}g%$ Cu, and $160.9{\mu}g%$ Zn in plasma was observed. Compared to the critical deficient values, 24.3, 10.3, 11.8, 28.7 and 0.0% of the surveyed cows obtained the respective elements in plasma below the standard levels. No significant difference (p >.05) in plasma concentrations of Ca, P, Mg and Zn for the low fertile (conception rate > 3) and fertile (conception rate < 3) cows was found. However, plasma Cu of the low fertile cows (averaged $77.2{\mu}g%$) was lower (p <.01) than that of the fertile ones (averaged $91.12{\mu}g%$). Additionally, it is observed that 47.1% of the cows with the low fertility problem obtained plasma Cu below the 65.0 pg% critical deficient value.

• Korean Journal of Veterinary Research
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• v.16 no.1
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• pp.65-69
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• 1976

A total of 736 Holstein cows were examined for incidence of infertility and 57 infertile cows of them were examined for blood values. The results obtained were as follows: 1. Fifty eight heads (7.9%) of 736 Holstein cows examined were proved to be infertile and the causative disease of 58 infertile cows were diagnosed as silent heat (31.0%), repeat breeder (20.8%), dysfunction of ovary (15.5%), lutein cyst (13.8%), endometritis (8.6%), follicular cyst (5.2%), cervicitis (1.7%), pyometra (1.7%), and free-martin (1.7%). 2. Among the data of blood values of 57 infertile cows, values of total serum protein and serum calcium were remarkably lower (p 0.01) than those of normal cows. Meanwhile the unbalanced Ca/P ratio (1.1) was observed.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.523-527
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• 1995

Two on farm feeding trials were conducted at the Pabna milkshed area during July to October 1993, to evaluate the response of feeding urea molassesed straw (UMS) on milk production of Pabna milking cows and its economics. A feeding trial of 60 days with 50 Pabna milking cows showed that the cows fed UMS as a replacer of dry straw increased daily milk yield by 1.37 litre. Moreover, it reduced the daily requirement of concentrate by 1.0 kg/head. In the second trial, a herd of 52 animals including 14 milking cows were fed UMS in place of traditionally used dry straw. Introduction of UMS increased the milk production by 1.0 litre/cow/day and saved concentrate by 1.5 kg/cow daily. Urea molassesed straw can safely and economically be fed to lactating cows.

• Journal of Veterinary Clinics
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• v.2 no.1
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• pp.133-141
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• 1985

In order to establish a method predicting susceptible cows to the parturient syndrome, various serum chemical parameters (calcium, phosphorus, Ca/P, magnesium, cholesterol, total protein, albumin, globulin, A/G, total lipid, non-esterified fatty acid(NEFA) and aspartate aminotransferase(AST)) were measured during late pregnancy and their relationships with periparturient diseases were investigated during puerpural period. The results obtained were as follows : 1. The factors affecting the prediction of susceptible cows to parturient syndrome were calcium, magnesium, total protein, globulin, A/G ratio and total lipid at 30 day antepartum and the diagnosability was 70.7%. 2. In the experimental cows producing more than 21kg of milk per day, the factors affecting the prediction of susceptible cows to parturient syndrome were calcium, NEFA and A/G ratio at 30 day antepartum and the diagnosability was 66.7%. 3. In the experimental cows calved more than 3 times, the factors affecting the perdiction of susceptible cows to parturient syndrome were calcium, total protein, albumin and NEFA at 30 day antepartum and the diagnosability was 83.3%.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.10
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• pp.1417-1429
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• 2004

The objective of the paper was to study the drinking and other related behavior of dairy cows (Bos taurus). There were 142 Holstein dairy cows observed and compared in this study. The experiment was designed on the basis of two different housing systems (wet pad with forced ventilation cooling house and open house); two different seasons (winter and summer); four different stages (high milk yielding cows, low milk yielding cows, dry cows, and heifers); and grouping (home and visitor animals). All cows had free access to water. Dairy cows spent 13.8 min/day drinking in wet-pad house and 11.7 min/day in open house. owever, there was no significant difference in the duration of water drinking between these two housing systems (p>0.05). The water consumption was significantly higher in wet-pad housed animals (68 L/day) than open-housed animals (31.5 L/day) (p<0.05). A significant interaction between housing and grouping (p<0.05) was found. Home and visitor animals spent more time drinking in open house, wet-pad house, respectively. A highly significant interaction was found between housing and drinking time during the day (p<0.001). Animals in open house drank more during the morning (6:00 to 10:00 h), whereas wet-pad housed animals drank in the afternoon (14:00 to 15:00 h) and evening (18:00 to 20:00 h). The average time a cow spent in drinking in summer was not ignificantly different from that of drinking in winter. However, the water intake was significantly higher in summer (61.9 L/day) than in winter (38.6 L/day) (p<0.05). Drinking activity showed a highly significant interaction between season and physiological stage (p<0.01). High milk yield cows spent more time drinking in summer than in winter, whereas cows in all other stages followed the opposite drinking pattern. Grouping exchange did not influence the drinking behavior of dairy cows in either season (p>0.05); both home and visitor animals spent almost the same time in drinking water. A strong significant interaction between season and time during the day was found(p<0.01), suggesting that animal's high drinking frequency occurred during the daytime for both seasons, with a peak midday in winter and two peaks at 10:00 h in the morning and 19:00 h in summer. Thus, drinking behavior was associated with the cooler time of day in summer and with the warmer hours of day in winter. High and low milk yielding cows and heifers spent 15.3 min/day, 14.3 min/day, and 12.8 min/day, respectively, in water drinking activity, but there was no significant difference among them (p>0.05). There was, however, a significant difference in water drinking activity found in dry cows, which spent less time in drinking at 8.2 min/day (p<0.05).

• Journal of Embryo Transfer
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• v.25 no.3
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• pp.133-139
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• 2010

The purpose of this study was to determine the relationship between conception rate and other parameters (body condition score; BCS, progesterone concentrations and follicle size) before estrus induction with $PGF_2{\alpha}$. The conception rate in cows with (2.75, 2.75 to 3.25 and 3.25), BCS regardless of AI (artificial insemination) time was 47.5, 67.5% and 48.5% at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of BCS was 59.0% in cows inseminated based on detected estrus, and 46.2% in cows inseminated at 72 to 80 hours (timed artificial insemination, TAI) after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 43.0% in cows with low progesterone concentrations (less than 1.0 ng/ml), and 67.5% in cows with high progesterone concentrations (more than 1.0 ng/ml) at $PGF_2{\alpha}$ injection. The conception rate regardless of progesterone concentrations was 59.9% in cows inseminated based on detected estrus, and 48.1% in cows of TAI after $PGF_2{\alpha}$ injection. The conception rate regardless of AI time was 36.0% in cows with small dominant follicles (less than 5 mm), 56.0% in cows between 5 mm to 10 mm of follicle size, and 65.5% in cows with large dominant follicles (more than 10 mm) at $PGF_2{\alpha}$ injection, respectively. The conception rate regardless of follicle size was 57.3% in cows inseminated based on detected estrus, and 47.6% in cows of TAI after $PGF_2{\alpha}$ injection. These results indicated that if the cows with BCS 2.75 to 3.25, active corpus luteum, and/or large dominant follicle (more than 10 mm) are used for estrus induction, the conception rate will be greater.

• Korean Journal of Veterinary Research
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• v.38 no.3
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• pp.642-651
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• 1998

The accuracy of ultrasonography for determining the presence of a functional corpus luteum in subestrous dairy cows was investigated, using a radioimmunoassay for progesterone in plasma. Luteal status (high or low progesterone concentrations) was diagnosed in 534 cows, using B-mode transrectal ultrasonography. Accuracy of ultrasonography was 96.3% and 88.8% in the cows with and without functional corpus luteum, respectively. In 362 cows diagnosed with functional corpus luteum by ultrasonographic examination, 20 cows were diagnosed with the non-functional corpus luteum by analysis of plasma progesterone concentrations (false positive). In 172 cows with non-functional corpus luteum by ultrasonographic examination, 13 cows were diagnosed with the functional corpus luteum based on plasma progesterone assay (false negative). Most of the corpus luteum with well-defined border and homogeneous echotexture were diagnosed with functional corpus luteum. All cows that were not detected a corpus luteum by ultrasonographic examination were diagnosed as non-functional corpus luteum. The corpus luteum of cows that were diagnosed with false positive appeared homogeneous echotexture and above 15 mm in diameter, but the corpus luteum was the non-functional corpus luteum within Day 5 (Day 0 is ovulation day) or after Day 19. The corpus luteum of cows that were diagnosed with false negative appeared heterogeneous echogenicity and below 15 mm in diameter, but the corpus luteum was the functional corpus luteum after Day 5 or around Day 17. It was concluded that accuracy of ultrasonography was excellent for determining the presence of a functional corpus luteum in subestrous dairy cows. The corpus luteum that was diagnosed with false positive or false negative was the developing or regressing states. Thus, ultrasonography was required a serial examination of two or three times accurately diagnosing these corpus luteum.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.9
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• pp.1267-1272
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• 2002

The present research was undertaken to monitor the effects of restricted and ad. libitum feeding during last trimester of pregnancy on the performance of crossbred dairy cows and their calves. For this purpose two groups of crossbred cows having eight animals in each group were put into two plane of nutrition during their last three months of pregnancy. Dietary energy level in one group was calculated as per Ministry of Agriculture, Fisheries and Food, (MAFF, UK) and the cows on other group received ad. libitum feeding (concentrate level similar to MAFF, recommendation+ad. libitum roughage). After calving, the cows of both groups were given same diet (ad. libitum) to study the effect of feeding during pregnancy on subsequent lactation performance of the cows. It was observed that during pre-calving period cows on ad. libitum feeding gained significantly (p<0.05) more body weight than that of cows on restricted feeding ($38.50{\pm}6.04$ vs $21.37{\pm}8.04kg/cow$). There was no significant differences between the birth weight of calves of restricted ($21.31{\pm}2.18kg$) and ad. libitum ($20.31{\pm}3.27kg$) groups. Dietary energy intake of ad. libitum group ($50.22{\pm}4.24$ MJ ME/d/cow) was significantly higher (p<0.01) than that of restricted group ($39.76{\pm}1.03$ MJ ME/d/cow). During lactation period cows that were on restricted feeding produced little more milk (3.06 lit/cow/day), than that of the cows on ad. libitum feeding (2.84 lit/cow/day). Although growth rate of the calves of restricted group upto one month ($0.23{\pm}0.09kg/calf/d$) was significantly (p<0.01) higher than that of ad. libitum group ($0.17{\pm}0.08kg/calf/d$) but after four months the growth rate of the calves of two groups become nearly similar ($0.35{\pm}0.02$ vs $0.37{\pm}0.03kg/calf/d$) and difference between them was non-significant. During lactation period cows of restricted group gained little weight but cows of ad. libitum group lost significantly (p<0.05) more weight. Per day metabolizable energy intake in both group during lactation period was nearly similar (67.54 vs 69.58 MJ ME). It is concluded that ad. libitum feeding during last trimester of pregnancy is neither economic for getting maximum milk yield nor for calf growth rate and MAFF, recommended level of dietary energy during that period could be applied on our pregnant crossbred dairy cows.