• 농업과학연구
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• 제41권4호
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• pp.335-339
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• 2014

Cowpea chlorotic mottle virus (CCMV)는 Group IV positive sense single strand RNA virus, Bromoviridae과, Bromovirus속으로 분류하는 식물병원성 바이러스로, 강낭콩(Phaseolus vulgaris), 나비완두(Clitoria ternatea), 담배(Nicotiana tabaccum), 대두(Glycine max), 동부(Vigna unguiculata, Vigna siensis) 및 땅콩(Arachis hypogaea)이 국내로 수입될 경우, 검사를 수행하는 관리급 검역바이러스이다. 본 연구에서는, RT-PCR, nested PCR 및 유전자-삽입 양성대조구를 개발하여, CCMV를 현장에서 신속, 정확하게 진단할 수 있는 정밀검정 시스템을 구현하였다. 본 연구에서 개발한 방법은 지속적으로 현장에서 활용되어 식물검역에 기여할 것이라고 기대된다.

• 한국균학회지
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• 제20권3호
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• pp.252-258
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• 1992

Czpaek agar을 이용하여, 동부품종의 콩열매에서, 11속 25종 1품종의 진균과 10% NaCl을 첨가한 배지에서 7속 18종 2품종의 진균을 분리하였다. 이때 분리된 진균의 코로니는 6716/g이였다. Czpaek 배지를 통하여 분리 동정된 균은 대부분이 Aspergillus, Pericillium, Emericella 및 Rhizopus속에 속하는 균들이였다. 위에서 소금에 내성이 강한 균으로 분리 동정된 균들은 A. favus, A. sydowii, A. tomari, A. flavipes, A. niger, A. flavar var. columnaris, A. ochraceus, A. oryzae 및 P. chrysogerism 이였다. 각각의 시료를 사용하여 Aflatoxin $B_1,\;B_2,\;G_1$ 및 $G_2$을 분석한 결과, 잔존된 것을 관찰하였다.

• 한국균학회지
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• 제44권1호
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• pp.61-63
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• 2016

2015년 9월 경상남도농업기술원 시험연구 포장에서 재배 중인 동부에 흰비단병 증상이 발생하였다. 병징은 동부의 줄기 지제부위가 수침상으로 물러지고 부패되어 서서히 시들면서 포기 전체가 말라 죽었다. 줄기의 병반부와 토양표면에 흰색의 곰팡이가 발생하며 갈색의 둥글고 작은 균핵이 많이 형성되었다. 감자한천배지에서 균총은 흰색으로 잘 자라며 배양기간이 경과함에 따라 갈색의 둥글고 작은 균핵을 많이 형성하였다. 균핵의 크기는 1~3 mm이며 균사의 폭은 $4{\sim}9{\mu}m$였다. 균사생육과 균핵형성 적온은 $30^{\circ}C$이었다. 담자균 균사특유의 clamp connection이 관찰되었다. 동부에서 발생한 병징과 병원균의 균학적 특징, internal transcribed spacer rDNA 염기서열을 비교 분석한 결과를 토대로, 이 병을 Sclerotium rolfsii에 의한 동부 흰비단병으로 명명하고자 제안한다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.141.1-141
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• 2003

Forty six isolates of bean common mosaic virus (BCMV) collected from azuki bean, mungbean, kidney bean, cowpea, broad bean and peanut were classified into three groups based on biological, serological, cytopathological, and molecular characteristics. Group I induced vein-banding symptoms in cowpea which was similar to those produced by the BCMV-cowpea strain. Group II caused mosaic symptoms in azuki bean but not in peanut and tobacco. Since this character was different from that of previously described BCMV strain, group II may not belong to BCMV GroupIII induced vein-clearing symptoms in azuki bean, kidney bean and peanut, which are typical symptoms for BCMV-peanut stripe virus strain. Virus inclusion patterns of BCMV groups were similar to those of Potyvirus subdivision III with the scroll, pinwheel and long laminated inclusions. However, the inclusions of laminated aggregates were never observed in mungbean isolates. Multiple alignment as well as cluster dendrograms of 3'noncoding region (3'-NCR) and a part of coat protein gene (CP) suggested that group I belongs to the BCMV-cowpea strain, group II to the BCMV-azuki bean strain, and group III to the BCMV-peanut stripe virus strain. Since molecular phylogenesis of BCMV based on nucleotides of 3'-NCR and coat protein differed from the grouping based on virulence differentiation, and BCMV groups are more closely related to each other with the same host origin, other characteristics of those strains are under investigation.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.193-193
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• 2022

Cowpea(Vigna unguiculata L. Walp) has a low fat and protein content and a relatively high starch content, so it is mainly used in various ways for song-pyeon sediment, rice-cake paste, and porridge. In Yeonggwang-gun, Jeollanam-do, cowpea for seed production is being cultivated for the localization of raw material for Rice cake with ramie leave. A cowpea variety 'Jang-Alchan' was improved from the cross between IT145373 and IT145391 at the JARES in 2019. 'Jang-alchan' has an erect plant with an intermediate plant habit, light purple-colored corolla, and heart-shaped leaflets. 'Jang-alchan' has an orange-yellow seed seed-surface of faint luster, and brown and straight pods when matured. The stem length of 'Jang-alchan' was 48cm, 9cm longer than that of the control cultivar 'Okdang' and the 100-seed weight was 14.1g. The weight ratio of the seed coat for the entire seed was 11.2%, which was 0.2%p lower than that of the control cultivar. Field resistance of 'Jang-alchan' to leaf and systemic diseases was similar to that of the control cultivar. Is field resistance of 'Jang-alchan' to lodging was slightly lower than that of the control cultivar. The average yield of 'Jang-alchan' was 1.85ton per hectare, which was 14% higher than that of the control cultivar 'Okdang', 'Jang-Alchan' does not require the installation of an espalier-net and is taller than the existing supplied 'Okdang', so it can be harvested by combine harvesting. In addition, it is expected to contribute to the localization of raw material grains for regional specialties as it can be cultivated over a large area.

• 한국작물학회지
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• 제59권3호
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• pp.332-340
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• 2014

본 연구는 동부나물 생산에 적합한 재배온도를 설정하고, 유전자원을 선발하고자 수행하였다. 재배온도 설정을 위해 Seowon을 이용하여 $15^{\circ}C$에서 $30^{\circ}C$까지 $3^{\circ}C$ 간격으로 처리하였다. 나물 생산에 적합한 유전자원의 선발을 위해 Seowon, IT154149, IT154153, Tvu7426, Tvu7778 등 12계통 및 품종을 이용하여 $27^{\circ}C$에서 재배하였다. 1. 동부나물 생산수율은 $27^{\circ}C$에서 657%로 가장 높았고, 재배온도가 낮아질수록 떨어졌다. 2. 비타민 C 함량은 $24^{\circ}C$ 재배에서 2.85 mg/g로 가장 많았고, 기타 재배온도에서는 2.15~2.29 mg/g 범위였으며, 재배기간이 길어질수록 증가하였다. 3. 동부나물의 무기성분 함량은 재배온도에 따른 차이가 없었고, 아미노산 함량은 $15^{\circ}C$에서 $24^{\circ}C$까지는 재배온도가 높아질수록 증가한 반면 $24^{\circ}C$에서 $30^{\circ}C$까지는 재배온도가 높아질수록 감소하였다. 4. 동부 자원별 나물 생산수율은 IT154153 647%, Seowon 615%, Tvu7426 608% 순으로 많았다. 동부나물의 생산수율이 높은 자원은 종실이 작고 종피가 얇으며 발아력이 우수한 특징을 나타냈다. 5. 동부나물의 무기성분 함량은 칼륨, 마그네슘, 칼슘, 나트륨, 철, 몰리브덴, 아연 순으로 많았다. 동부나물의 단백질, 칼슘, 아연, 몰리브덴, 철 등의 함량은 원료곡보다 많았고, 알루미늄, 붕소 등의 함량은 원료곡보다 적었다.

• Asian-Australasian Journal of Animal Sciences
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• 제9권4호
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• pp.411-420
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• 1996

Asia Triple HIn this study an attempt was made to determine the cost of producing fodders at Savar Dairy Farm (SDF). For this purpose, all seasonal fodders, such as Maize, Jowar, Cowpea and Oat and all perennial fodders, such as Para and Napier were studied. The highest acreage of land was allocated to high-land Para (33.8%) and the lowest acreage (2.7%) was devoted to Cowpea. Jowar attained the highest yield (74.2 tons) per hectare and Maize attained the lowest yield (25.8 tons/h). The highest cost per hectare was attributed to Jowar (TK. 20944.18) while the lowest cost was attributed to low-land para (TK. 10349.86). The cost of production of fodder per kilogram was the highest (TK. 0.66) for Maize and the lowest for Oat (TK. 0.24). The cost of production of low-land Para was much lower than that of high land Para. The per kilogram cost of silage production was the highest (TK. 0.71) for Maize and the lowest (TK. 0.31) was for Napier. The gross return analysis further showed that the highest net margin and B:C ratio were observed for Napier followed by Low-land Para, Jowar, Oat, Cowpea, High-land Para and Maize. Therefore, those fodders whose B:C ratios and yield/ha were higher should be allocated more area of land to stimulate increased returns to SDF in the future is suggested.

• The Plant Pathology Journal
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• 제19권3호
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• pp.166-170
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• 2003

Ninety samples showing mosaic symptoms on soybean (Glycine max) cv. Sukryangputkong were collected from the Cheongsongkun area, Kyungbuk province in Korea. Initially, DAS-ELISA was conducted far detection of Soybean mosaic virus (SMV). Negative samples were chosen at random and mechanically inoculated on soybean cv. Buffalo, which reported not to produce mosaic symptoms when mechanically inoculated with SMV. An isolate of SMV, designated as B-1, from Buffalo showing mosaic and mottle symptoms was used for identification and biological characterization of the causal vim. The purified B-1 isolate had spherical particles of approximately 24nm. It positively reacted with the antiserum against Cowpea mosaic virus (CPMV) but not with Cucumber mosaic virus (CMV) and SMV antisera. CPMV was newly isolated from soybean and had been characterized by host range and by serological and electron microscopic methods. Results of this study suggest that CPMV is the possible cause of mosaic disease in vegetable soybean and that based on sympto-matology, a difference between the typical mosaic and rugose symptoms caused by SMV and CPMV was observed. This is first report of CPMV from soybean in Korea.

• 한국작물학회지
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• 제46권4호
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• pp.341-343
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• 2001

Molecular markers have become fundamental tools for crop genome study. The objective of this study was to construct a genetic linkage map for cowpea with PCR-based molecular markers. Five hundred and twenty random RAPD primers were screened for parental polymorphism. Ninety RAPD markers from sixty primers was segregated in 75 F2 mapping population derived from the cross of local cultivars GSC01 and GSC02. 70 RAPD markers were found to be genetically linked and formed 11 linkage groups. Linkage map spanned 474.1 cM across all 11 linkage groups. There are six linkage groups of 40 cM or more, and five smaller linkage groups range from 4.9 to 24.8 cM. The average linkage distance between pairs of markers among all linkage groups was 6.87 cM. The number of markers per linkage group ranged from 2 to 32. The longest group 1 spans 190.6 cM, while the length of shortest group 11 is 4.9 cM. This map is further needed to be saturated with the various markers such as RFLP, AFLP, SSR and more various populations and primers. In addition, morphological markers and biochemical markers should be united to construct a comprehensive linkage map.

• 한국약용작물학회지
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• 제20권1호
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• pp.42-46
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• 2012

Soy isoflavones have been suggested to improve bone loss and lipid profile in postmenopausal women and ovariectomized rats. In present study, we investigated the hypothesis that consumption of soybean, mung bean, cowpea and azuki bean has a beneficial effect on lipid profile which associates with bone mass in ovariectomized rats. Forty two female Sprague-Dawley rats were either sham-operated (Sham) or surgically ovariectomized (OVX). Sham and OVX groups were fed a regular AIN-93M diet, but ovariectomized rats with soybean (OS), mung bean (OM), cowpea (OC) or adzuki bean (OA) were fed AIN-93M diet replacing 35% of corn starch with powdered OS, OM, OC or OA for 10 weeks. Total- and LDL-cholesterol concentrations were significantly lower in Sham and OC groups than other OVX groups. There was significant negative association between total cholesterol concentration and bone mineral density (BMD) of tibia in only OC group. In conclusion, total-cholesterol concentration was significantly and negatively correlated with BMD in rats consumed cowpea, suggesting that the reduced cholesterol concentration may have a beneficial effect on bone mass.