• 한국정보처리학회논문지
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• 제7권5S호
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• pp.1666-1675
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• 2000

본 논문에서는 전자상거래 시스템에서 요구하는 상호운용성과 실시간 요구사항들을 지원할 수 있는 실시간 웹 미들웨이(RTWM Real-Time Web Middleware) 프레임워크를 제안한다. RTWM시스템은 전자상거래 시스템의 실시간 요구사항들을 지원하기 위해CORBA 객체 서비스 구조를 확장하여 이벤트 모니터, 실시간 스케줄러, 실시간 이벤트 서비스 등의 객체 컴포넌트를 포함하는 구조를 가진다. 특히, 본 논문에서는 전자상거래 사용자의 실시간 요구사항을 지원할 수 있는 실시간 이벤트 필터링을 제공하는 것에 중점을 두었다. 사용자로부터 시간적 제약조건과 원하는 이벤트 정보를 입력받아 QoS저장소에 저장하고, 실시간 이벤트가 발생하며RTFA(Real-Time Fitering Agent)를 통해 소비자가 원하는 이벤트를 필터링하여 제공하게 된다. 이로써 사용자는 보다 높은 서비스를 제공받게 되고 이 과정을 통해 전체적인 네트워크 트래픽이 감소되는 효과를 얻을 수 있다.

• Structural Engineering and Mechanics
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• 제25권4호
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• pp.481-500
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• 2007

The dynamic instability of doubly curved panels, subjected to non-uniform tensile in-plane harmonic edge loading $P(t)=P_s+P_d\;{\cos}{\Omega}t$ is investigated. The present work deals with the problem of the occurrence of combination resonances in contrast to simple resonances in parametrically excited doubly curved panels. Analytical expressions for the instability regions are obtained at ${\Omega}={\omega}_m+{\omega}_n$, (${\Omega}$ is the excitation frequency and ${\omega}_m$ and ${\omega}_n$ are the natural frequencies of the system) by using the method of multiple scales. It is shown that, besides the principal instability region at ${\Omega}=2{\omega}_1$, where ${\omega}_1$ is the fundamental frequency, other cases of ${\Omega}={\omega}_m+{\omega}_n$, related to other modes, can be of major importance and yield a significantly enlarged instability region. The effects of edge loading, curvature, damping and the static load factor on dynamic instability behavior of simply supported doubly curved panels are studied. The results show that under localized edge loading, combination resonance zones are as important as simple resonance zones. The effects of damping show that there is a finite critical value of the dynamic load factor for each instability region below which the curved panels cannot become dynamically unstable. This example of simultaneous excitation of two modes, each oscillating steadily at its own natural frequency, may be of considerable interest in vibration testing of actual structures.

• 동아시아식생활학회지
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• 제13권2호
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• pp.73-82
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• 2003

In the early years of the Koryo dynasty(877~1392), the grain production was encouraged and the consumption of meat was abstained because of the Buddhism. Therefore, desserts including rice cos and cookies and teas were prevalent. Specially, the cooking skill of the desserts was highly developed because the desserts were the requisite of offered in Buddhist service and national ceremonies. Also, the king took the lead in abstaining to eat meat. According to $\boxDr$Koryodokyung$\boxUl$ , People in the early Years of the Koryo dynasty were unskilled to slaughter for serving meat to the envoy from China. Most ceremonies in Koryo dynasty were held fur retainers by king and the ceremonies held to celebrate the coronation, birth of the royal grandchildren, and royal birthday, and to treat the envoys and merchants from China(Song dynasty) and Tamra kingdom. The ceremonies were continuously held from the early year to the later year of Koryo dynasty. The aristocracy of the Koryo dynasty often held the extravagant ceremonies and drank liquor a lot in the ceremony and offered the extravagant foods such as oil-and-honey pastry and milk, which caused the national problem later. The royal religious ceremonies held often in the Koryo dynasty were ancestor worship ceremony, tea ceremony, lotus lantern ceremony, Palgwanhoe, etc. In Koryo dynasty, there were several government offices that took charge of royal dietary culture as follows: 1. Yomulgo (料物庫) - government office supplied with provisions 2. Sasunseo(司謄署) - government office that took charge of various kinds of side dishes 3. Saonseo(司酪署) - government office that took charge of wine and liquor 4. Naejangtaek(內莊宅) - government office managed paddy fields and dry fields owned by royal family 5. Sangsikguk(尙食局) - government office same as Sasunseo that took charge of various kinds of side dishes, the name changed to Sasunseo later 6. Sungwanseo (謄官署) - government office that took charge of foods for various religious services and ceremonies 7. Naewonseo (內園署) - government office that took charge of the garden

• Animal cells and systems
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• 제9권3호
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• pp.161-171
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• 2005

Integrin-linked kinase 1 (ILK1) regulates several protein kinases, including PKB/Akt kinase and glycogen synthase kinase ${\beta}$. ILK1 is also involved distinctively in the cell morphological and structural functions by interacting with the components of the extracellular matrix or integrin. According to the information of serum- and glucocorticoid-induced kinase 1 (SGK1) substrate specificity (R-X-R-X-X(S/T)-${\phi};{\phi}$ indicates a hydrophobic amino acid), two putative phosphorylation sites, $Thr^{181}\;and\;Ser^{246}$, were found in ILK1. We showed that ILK1 fusion protein and two fluorescein-labeled ILK1 peptides, $FITC-^{174}RTRPRNGTLN^{183}$ and $FITC-^{239}CPRLRIFSHP^{248}$, were phosphorylated by SGK1 in vitro. We also identified that 14-3-3 ${\theta}\;{\varepsilon}\;and\;{\xi}$, among several 143-3 isotypes $({\beta},\;{\gamma},\;{\varepsilon},\;{\eta},\;{\sigma},\;{\theta},\;{\tau}\;and\;{\xi})$ formed protein complex with ILK1 in COS-1 cells. Furthermore, the phosphorylation of $Ser^{246}$ by SGK1 induced the binding with 14-3-3. It was also demonstrated that 14-3-3-bound ILK1 has reduced kinase activity. Thus, these data suggest that SGK1 phosphorylates $Thr^{181}\;and\;Ser^{246}$ of ILK1 and the phosphorylation of its $Ser^{246}$ makes ILK1 bind to 14-3-3, resulting in the inhibition of ILK1 kinase activity.

• Archives of Pharmacal Research
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• 제29권11호
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• pp.1042-1048
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• 2006

Plasmid DNA vaccines encoding the hepatitis B virus (HBV) surface and hepatitis C virus (HCV) envelope antigens, respectively, were constructed, and attempt were made to find the possibility of a divalent vaccine against HBV and HCV. The expression of each plasmid in Cos-1 cells was confirmed using immunocytochemistry. To measure the induced immune response by these plasmids in vivo, female BALB/c mice were immunized intramuscularly with $100\;{\mu}g$ of either both or just one of the plasmids. Anti-HBV and HCV-specific antibodies and related cytokines were evaluated to investigate the generation of both humoral and cellular immune responses. As a result, specific anti-HBV and anti-HCV serum antibodies from mice immunized with these plasmids were observed using immunoblot. The levels of IL-2 and RANTES showing a $Th_{1}$ immune response were significantly increased, but there was no change in the level of IL-4 ($Th_{1}$ immune response) in any of the immunized groups. Compared with each plasmid DNA vaccine, the combined vaccine elicited similar immune responses in both humoral and cell-mediated immunities. These results suggest that the combined DNA vaccine can induce not only comparable immunity experimentally without antigenic interference, but also humoral and $Th_{1}$ dominant cellular immune responses. Therefore, they could serve as candidates for a simultaneous bivalent vaccine against HBV and HCV infections.

• Archives of Pharmacal Research
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• 제21권4호
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• pp.423-428
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• 1998

The ligand binding signals to a wide variety of seven transmembrane cell surface receptors are transduced into intracellular signals through heterotrimeric G-proteins. Recently, there have been reports which show diverse coupling patterns of ligand-activated receptors to the members of Gq family $\alpha$ subunits. In order to shed some light on these complex signal processing networks, interactions between G$\alpha$q family of G protein and neurokinin-2 receptor as well as muscarinic M$_{1}$ receptor, which are considered to be new thearpeutic targets in asthma, were studied. Using washed membranes from Cos-7 cells co-transfected with different G.alpha.q and receptor cDNAs, the receptors were stimulated with various concentrations of carbachol and neurokinin A and the agonist-dependent release of [$^3H$]inositol phosphates through phospholipase C beta-1 activation was measured. Differential coupling of Gaq family of G-protein to muscarinic M$_{1}$ receptor and neurokinin-2 receptor was observed. The neurokinin-2 receptor shows a ligand-mediated response in membranes co-transfected with G$\alpha$q, G$\alpha$11 and G$\alpha$14 but not G$\alpha$16 and the ability of the muscarinic $M_1$ receptor to activate phospholipase C through G$\alpha$/11 but not G$\alpha$14 and G$\alpha$16 was demonstrated. Clearly G$\alpha$/11 can couple $\M_1$ and neurokinin-2 receptor to activate phospholipase C. But, there are differences in the relative coupling of the G$\alpha$14 and G$\alpha$16 subunits to these receptors.

• Molecules and Cells
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• 제38권2호
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• pp.145-150
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• 2015

Continuous intra- and extracellular stresses induce disorder of $Ca^{2+}$ homeostasis and accumulation of unfolded protein in the endoplasmic reticulum (ER), which results in ER stress. Severe long-term ER stress triggers apoptosis signaling pathways, resulting in cell death. Neural epidermal growth factor-like like protein 2 (NELL2) has been reported to be important in protection of cells from cell death-inducing environments. In this study, we investigated the cytoprotective effect of NELL2 in the context of ER stress induced by thapsigargin, a strong ER stress inducer, in Cos7 cells. Overexpression of NELL2 prevented ER stress-mediated apoptosis by decreasing expression of ER stress-induced C/EBP homologous protein (CHOP) and increasing ER chaperones. In this context, expression of anti-apoptotic Bcl-xL was increased by NELL2, whereas NELL2 decreased expression of pro-apoptotic proteins, such as cleaved caspases 3 and 7. This anti-apoptotic effect of NELL2 is likely mediated by extracellular signal-regulated kinase (ERK) signaling, because its inhibitor, U0126, inhibited effects of NELL2 on the expression of anti- and pro-apoptotic proteins and on the protection from ER stress-induced cell death.

• KIEE International Transactions on Power Engineering
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• 제4A권4호
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• pp.171-177
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• 2004

In the current age of the information oriented society in which we live, many people use PCs and are dependant on the databases provided by the network server. However, online data can be missed during the occurrence of a blackout and furthermore, power failure can greatly effect Power Quality. This has resulted in the trend of using interruption-free live-line work when trouble occurs in a power system. However, 83% of the population receives an electric shock experience when a laborer is performing interruption-free live-line work. In the interruption-free method, education and training problems have been pinpointed. However, there are few instructors to implement the necessary training. Furthermore, the trainees undergo only a short training period of just 4 weeks. In this paper, to develop a method with no restrictions on time and place and to ensure a reduction in the misuse of materials, immersion type virtual reality (or environment) technology is used. The users of a 3D immersion type VR training system can interact with the system by performing the equivalent action in a safe environment. Thus, it can be valuable to apply this training system to such dangerous work as 'Interruption-free live-line work exchanging COS (Cut-Out-Switch)'. In this program, the user carries out work according to instructions displayed through the window and speaker and cannot perform other tasks until each part of the task is completed in the proper sequence. The workers using this system can utilize their hands and viewpoint movement since they are in a real environment but the trainee cannot use all parts and senses of a real body with the current VR technology. Despite these weak points, when we consider the trends of improvement in electrical devices and communication technology, we can say that 3D graphic VR application has high potentiality.

• 한국의류학회지
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• 제25권8호
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• pp.1444-1452
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• 2001

Textile materials are frequently in contact with surfactant solutions during their manufacturing or finishing processes as well as cleaning processes in use. Liquid wetting, wicking and absorbency of textile materials, and the liquid properties, surface characteristics and pore geometry of textile materials, and the liquie-solid interactions, In this paper, 10 different nonionic surfactants, including Span 20, Twen 20, 40, 60, 80, 21, 61, 81, 65, 85, were used. The surfactants were characterized by their hydrophile-lipophile-balance (HLB) values, structures, and surface tensions. The 0.1g/dL and 1.0g/dL surfactant solutions, which were both above critical micelle concentration (CMC), were used to see the concentration effects on the wetting and absorbency of cotton fabrics. The wetting behavior and liquid retention properties of hydrophobic cotton fabrics with different nonionic surfactant solutions are reported. The contact angles are greatly decreased and the water retention values are greatly increased by adding most of the surfactants studied into the system. The extents of this effects are influenced by the characteristics of surfactants and its solutions. Hydrophilic surfactants which have low number of carbon atoms or unsaturated hydrophobe structures are more effective in improving the wetting and absorbancy of hydrophobic cotton fabrics. The water retention of hydrophobic cotton fabrics has positive relations with $cos{\theta}$, adhesion tension and work of adhesion. The 1.0g/dL surfactant solutions show similar, but slightly improved wetting and absorbency characteristics of hydrophobic cotton fabrics compared to the 0.1g/dL surfactant solutions.

• Biomolecules & Therapeutics
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• 제8권3호
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• pp.276-280
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• 2000

In order to identify the domains of the G$_{\alpha}$16 subunit G protein that are responsible for its activation by the Interleukin-8 receptor, a serious of chimeras between G$_{\alpha}$16 and G$_{\alpha}$11 were assessed for their abilities to be activated by these receptors. Co-expression of IL-8 receptor and chimeras in which the carboxyl-terminal regions of G$_{\alpha}$11 were replaced from 30 up to 156 amino acid residues with the corresponding regions of G$_{\alpha}$16 demonstrated that C-terminal 156 amino acid residues of the G$_{\alpha}$16 were not sufficient to confer IL-8 receptor interaction specificity. Testing of a reciprocal serious of chimeras composed of G$_{\alpha}$16 sequences at the amino terminus and G$_{\alpha}$11 sequences at the carboxyl terminals revealed that sequences extending from the amino tar- minus to amino acid 209 of G$_{\alpha}$16 were sufficient to 7ndow the chimera with 75-80% of interaction specificity for 7-8-induced activation. These results suggest th,.7t combined interactions of the C-terminal 30 amino acid residues and certain domains extending from the arts.ino terminus to amino acid 209 of Gal 6 protein may be involved in its couplings to IL-8 receptor.tain domains extending from the arts.ino terminus to amino acid 209 of Gal 6 protein may be involved in its couplings to IL-8 receptor.