• The Journal of Internal Korean Medicine
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• v.23 no.1
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• pp.41-46
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• 2002

Background and Objectives ; This experimental study was conducted to evaluate an anti-stress effect of Gamisachi-tang(加味四七湯) on mice immobilized by stress. Methods : The experimental animals were immobilized by stress for 15 minutes, and administered 9mg/20g or 18mg/20g of Gamisachi-tang(加味四七湯) extract for seven days before they got stressed. The serotonin contents in the frontal cortex, hypothalamus, corpus striatum, hippocampus were measured by HPLC method in rat brain. Results : In frontal cortex, serotonin contents significantly decreased in both sample A and B group compared to the controlled group. In the hypothalamus, serotonin contents increased in both sample A and B group compared to the controlled group. In the corpus striatum, serotonin contents significantly increased in both sample A and B group compared to the controlled group. In the hippocamous, serotonin contents significantly increased in both sample A and B group compared to the controlled group. Conclusions : According to the above results, Gamisachi-tang(加味四七湯) had a significant impact on the changes in serotonin contents, which occurred in a separated part of mouse brain caused by stress.

• Korean Journal of Veterinary Research
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• v.40 no.1
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• pp.1-16
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• 2000

Nowadays, mongolian gerbil is notably utilized for the research of brain and water deprivation because of a congenital incomplete willis circle structure in the brain, audiogenic seizure in low noise, and special cholesterol metabolism without water absorption for a long time. In this study, we are intend to identify the morphological changes of the catecholaminergic neuron of brain according to the time lapse in the condition of long term water deprivation. 55 mongolian gerbil were divided 10 groups(control, 1, 2, 3, 4, 5, 10, 15, 20, 42th day water deprivation group), of which each group include 5 mongolian gerbils and 5 normal mongolian gerbils in control group were also used for brain atlas as a control. The brains were observed by the immunohistochemical stain using the TH, DBH and PMNT antibody. The results were as followings; 1. The nerve fibers of the TH-immunoreactive neuron were observed only in the and corpus striatum of the telencephalon. 2. Intensity of the immunostain of the nerve fiber in the cerebral cortex and corpus striatum was decreased gradually day by day after water deprivation. 3. The TH-immunoreactive nerve cells were observed in the paraventricular and periventricular nucleus of the 3rd ventricular in the hypothalamus of mongolian gerbil but the number of nerve cells were decreased from the first day of the water deprivation to the 10th day and increased until the 20th day, after than redecreased from the 20th day by the continuous water deprivation. The number of nerve fibers in this area were increased in the first day, but decreased from the 2nd day of water deprivation. The shape and density of the dopamine secreting cells in the brain of mongolian gerbil by the immunoreactive stain were changed in the continuous water deprivation. In this results, we can conclude that dopamine concerned in the water metabolism of mongolian gerbil, and mongolian gerbil could be used as an animal model for the research of water deprivation.

• Journal of radiological science and technology
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• v.40 no.4
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• pp.595-603
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• 2017

The study is to produced a brain phantom simulating corpus striatum, which can evaluate the progression of parkinson's disease, to investigate possibility of reducing the brain exposure dose to CT while maintaining optimal image quality during PET-CT examinations. CT scans were performed by varying tube voltage (100, 120 kVp) and tube current (80, 140, 200 mAs) with $^{18}F$ FP-CIT injected into the phantom's hot sphere and background (radioactivity ratio 3:1)(reference condition; 120 kVp, 140 mAs). Estimated effective dose was calculated by using conversion factor according to each condition, and image quality was evaluated by setting SNR and CRChot image evaluation factors. Experimental results showed that the predicted effective dose below the CT imaging reference condition was reduced by at least 10% and by up to 60%, and the predicted effective dose beyond the reference condition was increased by 40%. In addition, there was no significant difference between SNR and CRChot of PET images, and it was confirmed that brain dose decreased with decrease of tube voltage and tube current. At the same time, there was no significant change in the quality of the image in terms of SNR and CRChot despite the change in scan conditions. This fact suggests that the quality of the images acquired under the existing dose conditions can be obtained even at low dose conditions and it is expected that it will be possible to use the brain PET-CT scan as a basic data for the research on reduction of dose and improvement of image quality.

• Journal of Oriental Neuropsychiatry
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• v.9 no.2
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• pp.87-95
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• 1998

This study was aimed to evaluate the anti-stress effect of Soeuminsohabhyangwon on the mice in Cold and Swimming stress.In order to investigate the anti-stress effect of Soeuminsohabhyangwon in Cold and Swimming stressed mice, the serotonin contents were measured by HPLC method in various part of mouse brain The following results were observed. 1. In Cerebral Cortex of Frontal Lobe, the serotonin content was decreased in the Control group as compared with Normal group and the serotonin content was increased in the SHW group as compared with Control group. 2. In hypothalamus, the serotonin content was decreased in the Control group as compared with Normal group and the serotonin content was increased with statistical significance in the SHW group as compared with Control group. 3. In corpus striatum, the serotonin content was decreased in the Control group as compared with Normal group and the serotonin content was increased with statistical significance in the SHW group as compared with Control group. 4. In hippocampus, the serotonin content was decreased in the Control group as compared with Normal group and the serotonin content was increased with statistical significance in the SHW group as compared with control group.Base on the above results, it may be concluded that Soeuminsohabhyangwon are effective to reduce stress.

• Journal of Korean Neurosurgical Society
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• v.29 no.2
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• pp.155-166
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• 2000

This study was designed to investigate the underlying mechanisms for the temporal changes of the striatal dopamine D2 receptors in the rat model of parkinsonism. After injection of the 6-hydroxydopamine into the substantia nigra of adult rats, we measured the receptor binding capacity(Bmax), mRNA and protein of the D2 receptor at 2, 4 and 8 weeks. Following the lesion, mRNA and protein were elevated simultaneously on both sides of the striata. They showed more increase on the normal side at 2 and 4 weeks, and then they were almost equally abundant on both sides at 8 weeks. We also observed their increased production in the diffuse cortical and subcortical regions. The Bmax value also increased bilaterally in both striata, and was higher on the normal side at 2 weeks and then on the lesioned side at 4 and 8 weeks. These findings suggest that production of the striatal D2 receptor is regulated at the transcriptional level in this animal model. They also imply that this control may be mediated through a pathway which can have influence on the whole brain, rather than the local control of the dopamine content alone. The measured functional activity(Bmax) of the D2 receptor was not proportional to the amount of the receptor mRNA and proteins produced. This difference may be explained by the post-translational modification of the receptor proteins, which may be controlled by such factor as the local concentration of dopamine.

• Journal of International Academy of Physical Therapy Research
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• v.6 no.2
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• pp.846-851
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• 2015

This neurological damage accelerates the infection reaction of cells and apoptosis at the time of reperfusion after ischemia occurs. BCL-2/BCL-2 allogeneic begeminum has a function of suppressing the apoptosis of cells, and thus it is inferred that the susceptibility of cells to apoptosis is determined by the amount of allogeneic begeminum present which is determined based on the amount of BAX. Ischemia was induced in SD mice by occluding the common carotid artery for 5 minutes, after which blood was re-perfused. NEES was applied to acupuncture points, at 12, 24, and 48 hours post-ischemia on the joksamri, Hapgok. Protein expression was investigated through BAX antibody immuno-reactive cells in the cerebral nerve cells and Western blotting. The results were as follows: In the present study as well, as a result of observation of the change in the number of the BAX reaction cells after the inducement of GI, there was the aspect of most of the BAX reaction cells being observed in the corpus striatum area of the GI group 24 hours after the inducement of ischemia. This revealed the same results as those of previous studies in which the change in the number of BAX reaction cells occurred in all areas while ischemia was in progress. The change in the expression of BAX protein after 24 hours showed that there was a very significant reduction in the NEES group compared to the GI group (p<.01). As a result, a greatest amount of change in the number of BAX immunoreactive cells related to apoptosis 24 hours after ischemia appeared in the NEES group. This study that ischemia increases the expression of BAX that induces apoptosis. Thus, it is determined that ischemia is the main cause of the apoptosis of neurons, and this study reveals that low frequency needle electrode electrical stimulation has the effect of blocking the apoptosis of neurons by reducing protein related to the apoptosis of cells that has increased after ischemia has occurred.

• Journal of Oriental Neuropsychiatry
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• v.8 no.1
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• pp.49-68
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• 1997

This study aimed to evaluate the anti-stress effect of Yangsimtang and Yangsimtang+Siyup on the rats in immobilization stress.The experimental animals were immobilized in the stress box(5${\times}$5${\times}$20cm) for 12 hours in a day suring 3 days, and administered 1ｇ/100ｇ of Yangsimtang and Yangsimtang+Siyup and Siyup extract for 12 days before stress. The norepinephrine, epinephrine, dopamine and serotonin contents were measured by HPLC method in various part of rat brain.The following results were observed.1. In frontal cortex the norepinephrine content of control group was 561.${\pm}$24.46 ng/g brain tissue, that of saple 1 group was 430.8$\pm$41.2 ng/g brain tissue, and that of sample 2 group was 417.2$\pm$38.5 ng/g brain tissue. The differences was statistically significant.2. In corpus striatum, the norepinephrine content of control group was 561.3$\pm$27.3 ng/g brain tissue, and that of sample 1 group was 422.1$\pm$21.2 ng/g brain tissue, the dopamine content of control group was 1205.1$\pm$75.9 ng/g brain tissue, that of sample 2 group was 685.6$\pm$41.5 ng/g brain tissue. The differences was statistically significant.3. In hypothalamus, the norepinephrine content of control group was 1165.1$\pm$162.6 ng/g brain tissue, that of sample 2 group was 947.2$\pm$35.7 ng/g brain tissue. The differences was statistically significant.4. In hippocampus, the norepinephrine content of control group was 931.6$\pm$82.2 ng/g brain tissue, that of sample 1 group was 652.1$\pm$47.5 ng/g brain tissue, and that of sample 2 group was 627.4$\pm$31.2 ng/g brain tissue, the dopamine contrnt of control group was 315.4$\pm$28.4 ng/g brain tissue, that of sample 2 group was 208.5$\pm$23.7 ng/g brain tissue. The differences were statistically significant.Base on the above results, it may be concluded that Yangsimtang and Yangsimtang+Siyup are effective to reduce stress.

• Korean Journal of Exercise Nutrition
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• v.13 no.3
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• pp.203-209
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• 2009

Periventricular leukomalacia (PVL) is a common white matter lesion affecting the neonatal brains. PVL is closely associated with cerebral palsy (CP). It has been suggested that maternal or placental infection can induce damage to the neonatal brains. In the present study, we investigated the effects of treadmill running and swimming of rat pups on the GFAP and MBP expressions in the brains of rat pups with maternal lipopolysaccharide (LPS)-induced CP. The rats were divided into the six groups for experiment 1: the control group, the control with mild exercise group, the control with moderate exercise group, the LPS-treated group, the LPS-treated with mild exercise group, and the LPS-treated with moderate exercise group (n=6 in each group). The rats in the running groups were forced to run on a motorized treadmill for 30 min 5 times a week for 4 weeks. For experiment 2, the rats were divided into four groups: the control group, the LPS-treated group, the LPS-treated with swimming group, and the LPS-treated with treadmill running group (n = 5 in each group). The rats in the swimming group were made to swim for 30 min once a day for 5 times per week during 2 weeks. The rats in the treadmill running group were made to run for 30 min once a day for 5 times per week during 2 weeks. The present results showed that intracervical maternal LPS injection during pregnancy significantly increased GFAP expression in the striatum and significantly decreased MBP expression in the corpus callosum of rat pups. The present results also showed that treadmill running and swimming significantly suppressed GFAP expression and significantly enhanced MBP expression in the brains of rat pups with maternal LPS-induced CP. This effect of treadmill running was shown as equally both in the mild-intensity exercise and in the moderate-intensity exercise. The present study revealed that exercise, both the treadmill running and swimming, is effective for the treatment of astrogliosis and hypomyelination associated with CP. Here in this study, we showed that treadmill running and swimming are effective for alleviating the detrimental effects of CP.