• Korean Journal Plant Pathology
• /
• v.12 no.3
• /
• pp.297-301
• /
• 1996

옥수수 깜부기병균에 대한 살균제 활성검정시 실내 검정과 온실 유묘검정과의 상관정도를 조사하기 위하여 5가지 살균제를 공시하여 실내와 온실에서 깜부기병균에 대한 억제효과를 비교하였다. 실내검정방법으로 사용한 최소생육억제농도법, 저지원법, 소생자발아검정법의 결과는 모두 온실유묘검정에서 얻어진 결과와 상관이 높았으며 특히 저지원법은 유묘검정결과와 매우 높은 상관관계를 보였다. 따라서 저지원법과 같은 실내검정방법은 대량의 활성검정시 간이검정방법으로 유용하게 이용될 수 있으리라 생각된다.

• Korean journal of applied entomology
• /
• v.16 no.2 s.31
• /
• pp.87-92
• /
• 1977

A barley virus disease has been severe in central Korea since 1963. To investigate the causal virus, examination of host ranges, transmission by insect vectors and · electron microscopy were conducted. In electron microscopy, particles identical with northern cereal mosaic virus were observed. The size of baciliform particles ranged from 300nm to 370nm in length and 57-60nm in diameter. The virus was transmitted by the small brown planthopper Laodelphax striatellus (Fallen). The latent period in the vector was seven to nineteen days, with 10 days the most prevalent. Barley, corn, wheat, rye, and oats were susceptible to the virus when inoculated by the insect vectors. It was concluded that the disease agent of the barley disease in Korea is northern cereal mosaic virus. This is the first known report of this disease in Korea.

• The Korean Journal of Cytopathology
• /
• v.2 no.1
• /
• pp.1-7
• /
• 1991

Cytologic findings from five cases with variable types of Hodgkln's disease were reviewed with special emphasis on the Reed-Sternberg (R-S) cells and their variants. Typical R-S and Hodgkin's cells were mono- or binucleated, and nuclei had rounded smooth contour, Acidophilic prominent nucleoli with perinucleolar halo were conspicuous in comparison to typical Reed-Sternberg cells, L & H (lymphocytic and histiocytic) cells in the lymphocyte predominant type tended to show pop-corn like irregular nuclear contour and to lack the prominent nucleoli. Lacunar cells in the nodular sclerosis type had multilobated nuclei with prominent acidophilic nucleoli. There was no prominent perinucleolar halo in L & H and lacunar cells. In conjuction with the number of Reed-Sternberg cells and back ground findings observed on the smears, the characteristic features of R-S cells and their variants allowed to make typing of Hodgkin's disease.

• Research in Plant Disease
• /
• v.17 no.3
• /
• pp.369-375
• /
• 2011

Fusarium verticillioides (teleomorph: Gibberella moniliformis), a member of the Gibberellea fujikuroi species complex, causes rots of corn stalks and ears, and produces a group of mycotoxins known as fumonisins that are harmful to animals and humans. Here, we focus on the development of a species-specific PCR primer set for differentiating F. verticillioides from other fumonisin-producing Fusarium species belonging to the species complex, such as F. proliferatum, F. fujikuroi, and F. subglutinans that are frequently associated with corn. The specific primers (RVERT1 and RVERT2) derived from the nucleotide sequences of RNA polymerase II beta subunit (RPB2) gene amplified a 208 bp-DNA fragment from only F. verticillioides isolates among the potential fumonisin-producing species examined; all of these isolates were shown to carry FUM1 required for fumonisin biosynthesis. The PCR detection limit using this specific primer set was approximately 0.125 pg/${\mu}l$ genomic DNA of F. verticillioides. In addition, the F. verticillioides-specfic fragment was successfully amplified from genomic DNAs of corn samples contaminated with Fusarium spp. This primer set would provide a useful tool for the detection and differentiation of potential fumonisin-producing F. verticillioides strains in cereal samples.

• Research in Plant Disease
• /
• v.23 no.3
• /
• pp.256-261
• /
• 2017

The effects of insecticide and fungicide treatment were investigated to reduce mycotoxin contamination of corn (Zea mays L.) seeds. Deoxynivalenol and zearalenone contents were reduced in the treated seeds, but aflatoxin, ochratoxin A, fumonisin, and T-2 toxin were not effective by chemical treatments. The chemical treatment did not affect the growth of saprophyte, but inhibited the pathogenic fungi such as Fusarium verticillioides, F. graminearum and F. equiseti. Myotoxin contents at different harvesting time were compared. As the harvest time was delayed, both levels of deoxynivalenol and zearalenone and frequency of Fusarium spp. increased. However, the major nutrient contents of corn seeds were not affected by harvesting period. These results show that chemical treatments are necessary to reduce the fungal contamination of corn and harvest without delay is important as well.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.29 no.4
• /
• pp.329-336
• /
• 2009

This experiment was carried out to know adaptability and forage production and quality of corn hybrid for silage at paddy field from 2007 to 2008 at Chungnam province. Growth, forage production and quality of silage corn showed more well drained condition than poorly drained condition at paddy field. Among growth characteristics, 'Kwangpyongok' and 'DK697' hybrids were somewhat strong for waterlogging, then and good at stay green, lodging, disease and insect resistance. Fresh yield of 'DK697' hybrid at poorly drained paddy field was the highest as 32,610 kg per ha among corn hybrids. The dry yield of 'P32P75' hybrid at poorly drained paddy field was the highest as 14,910 kg per ha The result of this study showed that 'P32P75', 'DK697', 'Kangdaok' and 'Kwangpyongok' hybrids had good growth characters and forage productivity at poorly drained paddy field and dry matter yield at poorly drained paddy field was 65.6% level compared with well drained paddy field.

• Research in Plant Disease
• /
• v.7 no.1
• /
• pp.42-48
• /
• 2001

The effect of cultural, physical and chemical control methods on the population density of Meloidogyne arenaria second-stage juveniles (J2) and on fruit yields of oriental melon was investigated at Seongju Fruit Vegetable Experiments Station, Kyungpook province, Korea, for two years from 1999 to 2000. Crops used in a rotation prior to Oriental melon were rice, corn, sesame, and green onion. The physical methods used were either solarization, soil addition or soil drying, and a nematicide, fosthiazate of granular formula, was used as the chemical method, applying at a rate of 300g a. i./10 a. Growing rice in the rotation, solarization, and soil addition controlled the nematode disease most effectively, reducing the number of J2 by 90% and increasing fruit yields two times. However, the effects of these control methods on the J2 population were limited to the early growing season; the J2 population increased later, suggesting that additional control practices may be needed in the following season. The next effective control methods were use of corn in the rotation, the nematicide application, and soil drying. The nematicide application was effective only for the early fruit yield, but neither for the late nor for the total yields. Use of sesame or green onion in the rotation was not effective in controlling the nematode.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.6
• /
• pp.884-891
• /
• 2006

The effect of dietary vitamin E supplementation on immune responses was studied in breeder chickens during the maturing period. In experiment 1, 17-week old female birds were fed corn-soybean meal based diets supplemented with either 0, 40, 80, 120, or 160 mg vitamin E (all-rac-${\alpha}$-tocopherol acetate)/kg diet for 19 weeks. In experiment 2, 23-week old male birds were fed the corn-soybean meal based diet supplemented with either 0, 20, 40, 80 or 160 mg vitamin E/kg diet for 8 weeks. The chickens were evaluated for growth performance, antibody titer to sheep red blood cell (SRBC), Newcastle disease virus (NDV), infectious bursal disease virus (IBDV) and infectious bronchitis virus (IBV), and skin response to phytohemagglutinin-P (PHA-P). The results showed that supplemental vitamin E improved body weigh gain of laying pullets during peak-laying period but had no significant effect on growth performance of cockerels. For cockerels, addition of 20 mg vitamin E/kg diet significantly enhanced (p<0.05) immune response to SRBC compared to those added with 0, 80 and 160 mg vitamin E/kg diet; addition of 20 mg vitamin E/kg diet had higher (p<0.01) antibody titer to IBDV than those added with 40-160 mg vitamin E/kg diet. No significant effects on immune response were observed in laying pullets fed supplemental vitamin E. The findings suggest that moderate supplementation of vitamin E may enhance immune responses to selective antigens in cockerels but excessive vitamin E may depress specific immune response.

• Journal of Nutrition and Health
• /
• v.24 no.3
• /
• pp.179-188
• /
• 1991

Twenty college women were led experimental diet which composed ot basal diet plus different kinds of dietary rats at 27% Cal. Equal amount of 13.5g of corn oil, perilla oil or fish oil was supplied for 2 weeks as a source of n6 linoleic acid(LA). n3 $\alpha$-linolenic acid (LL). or n3 EPA + DHA. respectively. Plasma total Chol level was reduced by perilla and fish oils, significantly only by fish oil. Plasma Chol level was rather increased by corn oil(P<0.05), but was decreased by double amount of corn oil supplement. Therefore, hypocholesterolemic effect of fatty acids was in the order of n3 EPA+DHA>n3 LL>n6 LA and influenced by the degree of fat unsaturation. Plasma TG level was also significantly decreased by n3 EPA+ DHA and increased by n6 LA. Hypotriglyceridemic effect of fatty acids was also in the order of n3 EPA + DHA> n3 LL>n6 LA and influenced by the unsaturation. However, the reduction of plasma TG was more influenced by the fatty acid structure rather than the fat unsaturation. There were no significant effects on lipoprotein pattern 3nd chemical compositions of lipoprotein by different dietary PUFAs. but fish oil diet significantly increased the relative proportion of HDL-Chol. In conclusion. cholesterol- lowering effect of dietary PUFAS seemed to be a function of total fat unsaturation but hypotriglyceridemic effect seemed to be more linked to the ratty acid structure rather than the degree of unsaturation. The hypolipidemic effect of n3 PUFAs was significant so that fish oil or perilla oil may have important nutritional applications in the prevention and treatment of atherosclerotic disease.

• Journal of Nutrition and Health
• /
• v.31 no.9
• /
• pp.1394-1403
• /
• 1998

The study was designed to observe the effect of dietary calcium and fats on plasma cholesterol level, hepatic microsomal fluidity and HMG-CoA reductase activity as well as the excretion of fecal bile acids and neutral sterols in 1, 2-dimethylhydrazine(DMH)-treated rats. Male Sprague Dawley rats, at 7 weeks of age, were divided into 2 groups, 0.3% and 1.0% Ca levels and each group again subdivided into 2 groups of corn oil and perilla oil. Each rat was intramuscularly infused with DMH for 6 weeks to give total dose of 180mg/kg body weight and also fed experimental diet containing 15%(w/w) different fit and Ca(0.3% or 1.0%) for 20 weeks. High dietary calcium(1.0%) did not significantly influence on plasma cholesterol as well as hepatic microsomal fluidity and HMG CoA reductase activity, but significantly reduced the excretion of total bile acid per gram of faces and increased the excretion of total neutral sterol. However, high dietary Ca reduced the excretion of secondary bile acid(deoxycholic and lithocholic acids) which was known as promoter for colon cancer. Perilla oil rich in n-3 ${\alpha}$-linolenic acid significantly decreased plasma cholesterol by increasing hepatic microsomal fluidity compared with corn oil, but did not influence on HMG CoA reductase activity. Perilla oil did not influence on fecal excretion of total and primary bile acids, but reduced the excretion of secondary bile acids. Therefore, it could be recommended to consume more fish product and food rich in calcium and use more perilla oil in meal preparation to prevent from coronary hear disease and colon cancer especially when high fit diet has been practiced. (Korean Nutrition 31(9) : 1394-1403, 1998)