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Functional characterization of ABA signaling components using transient gene expression in rice protoplasts

  • Song, In-Sik;Moon, Seok-Jun;Kim, Jin-Ae;Yoon, Insun;Kwon, Taek-Ryoun;Kim, Beom-Gi
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.109-109
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    • 2017
  • The core components of ABA-dependent gene expression signaling have been identified in Arabidopsis and rice. This signaling pathway consists of four major components; group A OsbZIPs, SAPKs, subclass A OsPP2Cs and OsPYL/RCARs in rice. These might be able to make thousands of combinations through interaction networks resulting in diverse signaling responses. We tried to characterize those gene functions using transient gene expression for rice protoplasts (TGERP) because it is instantaneous and convenient system. Firstly, in order to monitor the ABA signaling output, we developed reporter system named pRab16A-fLUC which consists of Rab16A promoter of rice and luciferase gene. It responses more rapidly and sensitively to ABA than pABRC3-fLUC that consists of ABRC3 of HVA1 promoter in TGERP. We screened the reporter responses for over-expression of each signaling components from group A OsbZIPs to OsPYL/RCARs with or without ABA in TGERP. OsbZIP46 induced reporter most strongly among OsbZIPs tested in the presence of ABA. SAPKs could activate the OsbZIP46 even in the ABA independence. Subclass A OsPP2C6 and -8 almost completely inhibited the OsbZIP46 activity in the different degree through the SAPK9. Lastly, OsPYL/RCAR2 and -5 rescued the OsbZIP46 activity in the presence of SAPK9 and OsPP2C6 dependent on ABA concentration and expression level. By using TGERP, we could characterize successfully the effects of ABA dependent gene expression signaling components in rice. In conclusion, TGERP represents very useful technology to study systemic functional genomics in rice or other monocots.

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Chuna Manual Therapy for Stroke: A Systematic Review and Meta-analysis (뇌졸중의 추나요법에 대한 체계적 문헌 고찰)

  • Kim, Mi-Kyung;Han, Chang-Ho
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • v.14 no.2
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    • pp.15-28
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    • 2019
  • Objectives : This study aimed to systematically and comprehensively review controlled clinical trials on the effectiveness and safety of Chuna Manual Therapy for stroke. Methods : By October 7, 2019, three core databases and three domestic databases were searched. Seven major academic journals on the related field were also hand-searched. Methodological quality of the included studies was evaluated using the Cochrane risk of bias tool. Meta-analysis was conducted and the quality of its evidence was assessed using the GRADE methodology. Results : Five articles met the eligibility criteria. The results of most of included studies were in favor of Chuna Manual Therapy. No study described any adverse events during or after the clinical trials. Meta-analysis of three eligible studies showed that the pre- and post-treatment scores on the Modified Barthel Index (mean difference 8.00, 95% confidence interval 0.26 to 15.74) and Berg Balance Scale (mean difference 3.57, 95% confidence interval 0.64 to 6.51) of the Chuna-treated group were significantly different, but only marginally higher than those of the non-treatment group. Thus, the level of evidence gathered from these studies was assessed to be low. Conclusions : Based on current available evidence, any confirmative conclusions cannot be made on the effectiveness and safety of Chuna Manual Therapy for stroke because of the small sample size, low methodological quality, presence of statistical heterogeneity, and missing safety information. More rigorously designed large-scale multi-center studies are needed to establish more specific and credible evidence to support or oppose the use of Chuna Manual Therapy for stroke.

An experimental study on swirling spray flame structure by air-blast nozzle (기류분사 노즐에 의한 선회 분무 화염의 구조에 관한 실험적 연구)

  • O, Sang-Heon;Baek, Min-Su;Kim, Dong-Il
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.21 no.4
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    • pp.473-485
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    • 1997
  • Detailed experimental study has been made of air blast kerosene spray flames with and without swirl in combustion air flow. Phase-Doppler detect technique is used to measure Sauter mean diameter, axial component mean and rms velocity, size-velocity correlation, and number density. These measurements are obtained for both nonreacting and reacting cases under several stable flame conditions. The results show that the introduction of swirl to the combustion air modifies the spatial distribution of droplet size, velocity, and number density, and thus alters the flame structure. However, due to the weak swirl intensity, the overall structure of swirling flames are essentially same as that of nonswirling flames. Physical model of structure of air blast atomized spray flames is projected to show that spray flames are composed of three distinct regions: the two-phase mixture region, the main reaction and the intermittent combustion region. Near the atomizer, two phase mixture of droplet and air is formed in the core region. This dense spray region is characterized by high droplet number density and the strong convective effect. There follows the main combustion region where the main flame penetrates within the spray boundary. Main reaction region of these flames are governed by internal group combustion mode. Finally there exists the intermittent combustion region where local group burning or isolated droplet burning occurs.

Focus Group Study on Psychosocial Distress of Cancer Patients (치료 중인 암 환자들의 심리사회적 어려움에 관한 포커스 그룹 연구)

  • Yi, Myung-Sun;Kim, Jong-Heun;Park, Eun-Young;Kim, Jong-Nam;Yu, Eun-Seung
    • Korean Journal of Adult Nursing
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    • v.22 no.1
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    • pp.19-30
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    • 2010
  • Purpose: The purpose of the study was to describe psychosocial difficulties experienced by cancer patients. Methods: Three focus group interviews were conducted to collect the data from 19 outpatients with cancer undergoing treatments during 2009. All interviews were audio-recorded and transcribed verbatim. The data were analyzed using constant comparative analysis of grounded theory. Results: As a result of constant comparative analysis, 'psychosocial distress' was identified as a core category, and seven subcategories were identified. 'Plunged into negative emotions' and 'damaged self-identity' were identified as major categories in the individual dimension. 'Difficulty in dealing with diagnosis' and 'hasty expectations and concerns' were identified in the dimension of family. 'Inefficient communication' and 'lack of necessary information' were identified in the dimension of health care setting. 'Lonely journey into a strange territory' was identified in the dimension of society. Conclusion: The results of the study indicate that cancer patients experience a wide range of psychosocial problems. Thus, in assessing and relieving psychosocial distress of cancer patients, it is necessary to focus not only on the level of the individual but also on the levels of family, health care setting, and society in general is necessary.

A Seamless Multicast Scheme to Prevent Packet Loss in Proxy Mobile IPv6 Networks (Proxy Mobile IPv6 네트워크에서 패킷 유실을 방지하는 끊김 없는 멀티캐스트 기법)

  • Kim, Jong-Min;Kim, Hwa-Sung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.1B
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    • pp.7-20
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    • 2011
  • Recently, Proxy Mobile IPv6(PMIPv6) networks have been studied as the mobility management protocol to effectively use limited wireless resources. And the multicasting, which is core technology of the Internet broadcast system such as mobile IPTV, has been discussed mainly based on PMIPv6 network. However, multicasting based on PMIPv6 network causes disconnection of services because it does not solve problems of packet loss during binding and group joining procedure. Hence, we propose a seamless multicast scheme which prevents packet loss in PMIPv6 networks. The proposed scheme achieves lower latency than the existing scheme because it combines binding and group joining procedure, a1so it does not cause about packet losses due to performing buffering. We proved the performance using the simulations.

A Seamless Multicast Scheme Supporting Global Mobility in Proxy Mobile IPv6 Networks (프록시 모바일 IPv6 네트워크에서 전역 이동을 지원하는 끊김 없는 멀티캐스트 기법)

  • Kim, Hwan-Gi;Kim, Jong-Min;Kim, Hwa-Sung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.38A no.3
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    • pp.258-267
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    • 2013
  • Recently, Proxy Mobile IPv6(PMIPv6) networks have been drawing attention as the mobility management protocol that uses limited wireless resources effectively. And the multicast, which is a core technology of the Internet broadcast system such as mobile IPTV, has been widely discussed mainly based on PMIPv6 network. However, PMIPv6-based multicast cannot support the global mobility directly between different PMIPv6 domains because PMIPv6 is basically designed for local mobility in single PMIPv6 domain. Moreover, PMIPv6-based multicast causes the disconnection of services because it does not solve the packet loss problem during binding and group joining procedure. In this paper, we propose a global mobility scheme that supports the seamless multicast service in PMIPv6 networks. The proposed scheme supports the global mobility due to the addition of extra signalling messages between LMAs. Also, it achieves low latency because it performs fast binding and group joining procedure. We present the simulation results which show that the proposed scheme achieves the global mobility with low latency through the NS-2 simulation.

Characteristics of Acupuncture at LU10 on Abdominal Thermography of Health Subject (어제(魚際)(LU10) 자침(刺鍼)이 복부(腹部) 한열변화(寒熱變化)에 미치는 영향(影響))

  • Jeon Moon-Kee;Kim Jae-Hyo;Byun Kee-Won;Do Jin-Woo;Kim Kyung-Sik;Sohn In-Cheul
    • Korean Journal of Acupuncture
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    • v.20 no.2
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    • pp.1-20
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    • 2003
  • This study was examined for characteristics of acupuncture of LU10 on the abdominal thermography of health subject. The volunteers who participating in this study had taken rest for 20 - 30 mins in room temperature $(23-25^{\circ}C)$ before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of abdomen including a below part of the chest was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co., Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to both LU10 for 30 mins. The results showed that acupuncture of LU10 for 30 mins had more potencies of changes on all the ROIs of abdominal thermography than those of control group. Also, those responses were significantly clear at the A1, A3, A6, A7 and A9 areas. It was observed that the quantities of thermal changes following acupuncture of LU10 been increased significantly comparing that of control group at the all ROIs (region of interest). These effects have more potencies at the upper (A1 and A2) and below (A7, A8, A9) abdominal areas than midline ones (A4, A5, and A6). These results suggest that acupuncture of LU10 having the characteristics of controls of chill and fever (寒熱) may modulate thermal distributions and changes of abdominal areas including the below of chest.

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Chemotaxonomic and Phylogenetic Study on the Oligotrophic Bacteria Isolated from Forest Soil

  • Whang, Kyung-Sook
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 2000.04a
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    • pp.150-156
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    • 2000
  • Oligotrophic bacteria isolated from forest soil showed a specific community consisting of various taxonomic groups compared with those in other soil or aquatic habitats. Based on the cell shape, the isolates were divided into four groups: regular rod, curved/spiral rod, irregular rod, and prosthecate bacteria. The cellular fatty acids 60 oligotrophic isolates were analyzed. The 30 fatty acids which were identified or characterized are classified. At the dendrogram based on cellular fatty acid composition, four clusters(I-IV) were separated at a euclidian distance of about 50. Cluster 3 and 4-a strains were containing Q-8, these strains are accommodated in the Proteobacteria gamma and beta subdivision. The chemotaxonomic profiles of the cluster 4-a strains showed good agreement with those of the genus Burkholderia. Cluster 3 was characterized by the presence of branched-chain fatty acids, iso-C15:0, iso-C17:1, and iso-C17:0 as the major components. These chemotaxonomy suggested the close relationship of the isolates with Xathomonas/Sterotrophomonas group. Based on the 16S rDNA sequence analysis, the two representative strains(MH256 and MA828) of cluster 3 showed the close relation to genera, Xathomonas/Sterotrophomonas, but were not included in these genera. These strains were even further away from core Xanthomonas, and clearly were seen to branch outside the cluster formed by the Sterotrophomonas maltophilia. MH256 and MA828 16S rDNA sequence was different enough to put new genus on a separate branch. The isolates with Q-10 were also studied. They are corresponded to the two large groups in Proteobacteria alpha subdivision. One was incorporated in the genus Bradyrhizobium cluster, which also includes Agromonas, a genus for oligotrophic bacteria. The strains of the other group showed high similarity to the genus Agrobacterium.

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Characteristics of Acupuncture at ST36 on Facial Thermography of Health Subject (족삼리(足三里) (ST36) 자침(刺鍼)이 안면부(顔面部) 한열변화(寒熱變化)에 미치는 영향(影響))

  • Kim Yong-Tae;Kim Jae-Hyo;Hwang Jae-Ho;Kim Kyung-Sik;Sohn In-Cheul
    • Korean Journal of Acupuncture
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    • v.19 no.2
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    • pp.13-33
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    • 2002
  • This study was examined for effects of acupuncture of Zusanli (ST36) on the facial thermography in health subjects. The volunteers who participating in this study had taken rest for 20 - 30 mins in room temperature (23-$25^{\circ}C$) before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of face was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co., Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to the left ST36 for 30 mins. The results showed that acupuncture of ST36 significantly decreased the temperature of all the areas of facial surface comparing to those of control group. Also, it was observed that the quantities of thermal changes following acupuncture of ST36 been increased significantly at the A1, A4, A6, A7 and A9 ROIs (region of interest) comparing that of control group. Observed the thermography classified by ROI, it was clear the fact that acupuncture of ST36 could modulate the specific areas concerning to the facial pathway of Stomach Meridian, because the thermal responses following acupuncture of ST36 were specific at the A1, A2, A5 and A9 ROIs, relatively. These results suggest that acupuncture of ST36 may modulate thermal distributions and changes of facial areas concerned with Stomach Meridian.

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Effects of Zusanli (ST36) Acupuncture on Abdominal Thermography of Health Subject (족삼리(足三里) (ST36) 자침(刺鍼)이 복부(腹部) 한열변화(寒熱變化)에 미치는 영향(影響))

  • Sim Won-Bo;Kim Jae-Hyo;Kim Jong-Sung;Song Jae-Soo;Kim Kyung-Sik;Sohn In-Cheul
    • Korean Journal of Acupuncture
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    • v.20 no.1
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    • pp.1-20
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    • 2003
  • This study was examined for effects of acupuncture of Zusanli (ST36) on the abdominal thermography of health subject. The volunteers who participating in this study had taken rest for 20-30 mins in room temperature (23-$25^{\circ}C$) before the examination and informed them what to prohibit smoking, drinking and administration of drug for the previous day. The thermography of abdomen including a below part of the chest was taken using Infra-Red Imaging System (IR 2000, MEDI-CORE Co, Korea) by time interval of 15 minutes at 15 min before, just before and 15 min after, 30 min after and 45 min after acupuncture stimulation. Acupuncture was applied to the left ST36 acupoint for 30 mins. The results showed that acupuncture of ST36 significantly had more potencies of changes on all the of abdominal thermography than those of control group. Also, it was observed that the quantities of thermal changes following acupuncture of ST36 been increased significantly comparing that of control group at all the ROIs (region of interest). Observed the thermography classified by ROI, however, it was failed that acupuncture of ST36 could modulate the specific areas concerning to the abdominal pathway of Stomach Meridian. These results suggest that acupuncture of ST36 may modulate thermal distributions and changes of abdominal areas including the below of chest.

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