• 제목/요약/키워드: conventional medicine

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Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2000년도 The 7th International Symposium
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    • pp.114-115
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    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will bel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field frill be presented. And some problems arising for the somatic embryogenesis system will be also discussed.

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직접형광항체법(直接螢光抗體法)에 의한 축산식품중(畜産食品中)의 Salmonella 균(菌) 검출(檢出)에 관한 실험적(實驗的) 연구(硏究) (Experimental Studies on Detection of Salmonellae in Animal-origin Foods by Means of Dirct Fluorescent Antibody Technique)

  • 전무형;차연호;정길택
    • 대한수의학회지
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    • 제14권2호
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    • pp.243-252
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    • 1974
  • The experiment was performed in order to investigate the applicability of the rapid detection of salmonellae in various animal-origin foods by means of the direct fluorescent antibody technique. Egg, sausage and chicken were inoculated with various concentrations of Sal.paratuphi A, Sal. paratyhi B and Sal. thompson, and the fluorescent antibody technique was applied and compared with the conventional cultura method for the sensitivity of detection of the organisms. Two methods were employed in the fluorescent antibody technique; the direct smear method in which the smear being made directly from the specimens, and the enrichment smear method in which the smear being made from the enrichment broth. The effect of various enrichment time (1,5,8,11 and 13 hours) in tetrathionate broth on the detection of salmonellae in the fluoresent antibody technique was also studied. The results obtained were summarized as followings; 1. Of the three methods, the enrichment smear method of fluorescedt antibody technique was highly effective as cultural method for the detection of salmonella organisms. 2. Direct smear method of fluorescent antibody technique was effective as two other methods $5{\times}10^4$ organisms presented in 50 g(ml) of specimens. This method may not be applicable when the specimens contained $5{\times}10^2$ or less organisms. 3. Of the three specimens, the recovery rate of Salmonella organisms from egg was slightly higher than that of sausage and chicken. 4. In fluorescent antibody technique and cultural method, the specimens inoculated with Sal. thompson were found to be higher detection rate than the specimens inoculated with Sal. paratyphi A, 5. The optimum enrichment time of Salmonella organisms in tetrathionate broth on the detection by fluorscent antibody technique was found to be 11 hours or longer when the specimens of egg, sausage and chicken were inoculated with approximately 500 organisms. The longer enrichment time was the higher detection rate up to 11 hours tested.

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근위골절술을 위한 Staple 설계 (A Newly Designed Miniplate Staple for High Tibial Osteotomy)

  • 문무성;배대경
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1995년도 춘계학술대회
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    • pp.19-22
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    • 1995
  • A biomechanical study was made to demonstrate the superior mechanical performance of the newly designed Miniplate staple to the conventional Coventry staple in high tibial osteotomy(HTO). Using twenty fresh porcine tibiae, the fixational strengh of the two different types of staple in HTO was compared. To minimize the error due to the specimen-to-specimen individuality, the bone mineral density of the tibiae was measured with a bone densitometry (Dual photon absorptionometer, Luner, USA) and those with $0.8\;{\sim}\;1.2\;gm/cm^2$ at the proximal tibia was used in the biomechanical test. Testing was performed on a material testing system (Autogram ET-5, Shimatzu, Japan) with aid of a commercial data processor (IBM 80386/ ASYST). Using two differant loading modes, 'pull-out' and 'push-out', the maximum resistant force required to release the staple from the substrate bone was recorded. In the pull-out test, ten non-osteotomized specimens were used and the staple was pullout by subjecting an axial tension on the head of the staple inserted. While in the pull-out test where ten tibiae osteotomized in the usual way of HTO were used, the staple was not directly loaded. In this testing, as a mimic condition of the natural knee, the distal part of the specimen tibia was pushed horizontally in order for the staple to be pulled out while the proximal tibia was fixed. The pull-out strength of Coventry staple and miniplate staple were found to be $27.88\;{\pm}\;5.12\;kgf$ and $182.47\;{\pm}\;32.75\;kgf$, respectively. The push-out strength of Coventry staple and miniplate staple were $18.40\;{\pm}\;4.47\;kgf$ and $119.95\;{\pm}\;19.06\;kgf$, respectively. The result revealed that miniplate staple had the pull-out/ push-out strength at least fivetimes higher than Coventry staple. Based on the measured data, it was believed that the newly designed miniplate staple could provide much better postoperative fixation in HTO. The postoerative application of long leg casting may not be needed after HTO surgery.

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약용 식물을 첨가한 머루주의 이화학적 특성과 발효 품질 평가 (Evaluation of Physicochemical and Fermentation Qualities of Moru Wines Supplemented with Pine Needles or Medicinal Herbs)

  • 이제혁;강태호;엄병헌;손은화;한우철;지설희;장기효
    • 동아시아식생활학회지
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    • 제22권6호
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    • pp.886-894
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    • 2012
  • For the development of Moru wine with enhanced sensory qualities, Moru (Vitis amurensis) wines were fermented with pine needles from Pinus densiflora Siebold et Zuccarini to make Moru-pine wine or medicinal herbs from Astragali Radix and Viscum album to make Moru-herb wine. Moru without pine needles or medicinal herbs was included as a control. Pine needles and Astragali Radix/Viscum album delayed the fermentation of Moru wine, but after 40 days of fermentation and aging, final ethanol contents, pH, acidity, and the sugar/organic acid content in these kinds of Moru wines had no differences. The final ethanol level and acidity of Moru wines were 11.5~12.9% and 1.1~1.2%, respectively, but any sugars (glucose, fructose and sucrose) were not detected in all Moru wines. These results are consistent with the general characteristics of Moru wines, which have a high acidity and low sugar contents. Relatively low browness and antocyanins were detected in Moru-herb wine decreasing its chromaticity in a sensory test. Overall, the supplements of Astragali Radix/Viscum album reduced the color of normal Moru wine, which might be applied toward the development of conventional Moru wines.

Assessment of endodontically treated teeth by using different radiographic methods: an ex vivo comparison between CBCT and other radiographic techniques

  • Demiralp, Kemal Ozgur;Kamburoglu, Kivanc;Gungor, Kahraman;Yuksel, Selcen;Demiralp, Gokcen;Ucok, Ozlem
    • Imaging Science in Dentistry
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    • 제42권3호
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    • pp.129-137
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    • 2012
  • Purpose: To compare different radiographic methods for assessing endodontically treated teeth. Materials and Methods: Root canal treatments were applied in 120 extracted mandibular teeth, which were divided into four groups: (1) ideal root canal treatment (60 teeth), (2) insufficient lateral condensation (20 teeth), (3) root canals filled short of the apex (20 teeth), (4) overfilled root canal treatment (20 teeth). The teeth were imaged using intraoral film, panoramic film, digital intraoral systems (CCD and PSP), CCD obtained with portable X-ray source, digital panoramic, and CBCT images obtained at 0.3 $mm^3$ and 0.2 $mm^3$ voxel size. Images were evaluated separately by three observers, twice. Kappa coefficients were calculated. The percentage of correct readings obtained from each modality was calculated and compared using a t-test (p<0.05). Results: The intra-observer kappa for each observer ranged between 0.327 and 0.849. The inter-observer kappa for each observer for both readings ranged between 0.312 and 0.749. For the ideal root canal treatment group, CBCT with 0.2 $mm^3$ voxel images revealed the best results. For insufficient lateral condensation, the best readings were found with periapical film followed by CCD and PSP. The assessment of teeth with root canals filled short of the apex showed the highest percentage of correct readings by CBCT and CCD. For the overfilled canal treatment group, PSP images and conventional periapical film radiographs had the best scores. Conclusion: CBCT was found to be successful in the assessment of teeth with ideal root canal treatment and teeth with canals filled short of the apex.

내과 중환자실 환자의 혈당조절 프로토콜 적용 및 효과 (Application and Effects of a Blood Glucose Control Protocol for Medical Intensive Care Unit Patients)

  • 김은성;최스미;김영삼
    • 중환자간호학회지
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    • 제7권2호
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    • pp.45-57
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    • 2014
  • Purpose: The purpose of this study was to examine the effect of a blood glucose control protocol for medical intensive care unit (ICU) patients. Methods: The subjects were recruited from medical ICU adult patients whose blood glucose levels exceeded 200 mg/dL in two consecutive tests. The experimental group (n=62) received the modified Yale (MY) insulin protocol, whereas the control group (n=64) was treated with the conventional insulin therapy methods. Results: In the experimental group, the mean blood glucose levels (p<.001) and the time to reach the target range of glucose (p<.001) decreased significantly while the incidence rates of a target range of glucose of 100-140 mg/dL (p<.001) increased significantly as compared to the control group. However, no statistically significant differences were found in the incidence of hypoglycemia(p=.644), or the number of glucose tests (p=.236) between the groups. The length of stays in the ICU (p=.001), ventilator care days (p=.038), and the Sequential Organ Failure Assessment (SOFA) score (p=.029) in the experimental group were significantly lower than those of the control group. Conclusion: Application of the protocol was effective in improving the state of blood glucose control in medical ICU patients. Therefore, this protocol is expected to be used as a part of nursing intervention in critical care nursing.

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Effects of Oketani Breast Massage on Breast Pain, the Breast Milk pH of Mothers, and the Sucking Speed of Neonates

  • Cho, Jeong-Sug;Ahn, Hye-Young;Ahn, Suk-Hee;Lee, Myeong-Soo;Hur, Myung-Haeng
    • 여성건강간호학회지
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    • 제18권2호
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    • pp.149-158
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    • 2012
  • Purpose: This study was done to test the effects of Oketani breast massage on breast pain and breast milk pH of mothers, and sucking speed of neonates. Methods: A nonequivalent control group and a pretest-posttest design was used. Postpartum mothers complaining of breast pain were recruited at a postpartum care center. The application of Oketani breast massage by an Oketani massage therapist was the experimental treatment. The control group received the conventional massage technique from a nurse at the postpartum care centre. The collected data were analysed using a $x^2$-test and a t-test with the SPSS WIN 12.0 program. Results: The participants were homogeneous in age, gestation period, and birth weight. Breast pain (t=8.384, p<.001) was significantly relieved, and breast milk pH (t=4.793, p<.001) was significantly increased in the experimental group compared to the control group. The sucking speed of the neonates in the experimental group was significantly increased compared to the control group (t=9.920, p<.001). Conclusion: These findings indicate that Oketani breast massage is effective in relieving breast pain and increasing breast milk pH as well as the sucking speed of neonates.

Developing a mass propagation technique for Aralia elata via somatic embryogenesis

  • Moon, H.K.;Lee, J.S.;Kim, T.S.
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2000년도 제7차 국제 심포지움(생약자원개발에 관한연구) 및 추계정기 학술발표회 초록집
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    • pp.16-17
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    • 2000
  • Aralia elata is found in mountain areas all over Korean peninsula. Aralia elata is the scientific name for Japanese angelica tree. The tree belongs to the family Araliaceae, commonly known as ginseng family. Bud sprouts from apical shoot tip of the plants are rich in flavor and thus mainly used for both folk medicine and vegetable. The stalks with apical buds are gathered in the early spring and planted in sandy soil or water in the greenhouse. The sprouting buds are then collected and sold as fresh vegetable. Although the plants have been used for food, they have been cultivated in a very small scale. In spring, local farmers just go around mountain areas to search the trees and gather the stalks as much as they get and sell them to the market. No conservation efforts have been made to stop the exploitation or to save the dwindling population. We tried to provide local farmers with the plants that may be used as an alternative to stalks from wild populations. This will hel! p conserve the wild populations. However, it is hard to propagate them either by conventional cuttings or by seed germination in a short period of time. Mass propagation using tissue culture systems have shown a great promise with several woody plants. Recently we developed a mass propagation technique via somatic embryogenesis system using mature and/ or juvenile explants for Aralia elata. Several factors affecting somatic embryogenesis system including SE(somatic embryo) induction, embryogenic callus proliferation, SE germination, plant regeneration and transplanting to field will be presented. And some problems arising for the somatic embryogenesis system will be also discussed.lso discussed.

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Antifungal Activity of Lagenaria breviflora Fruit Extracts Against Wood Rotting Fungi on Vitex doniana Wood

  • Adedeji, Gabriel Adetoye;Eguakun, Funmilayo Sarah;Elufloye, Taiwo Olayemi;Uriel, Tamunobubeleye
    • Journal of Forest and Environmental Science
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    • 제33권4호
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    • pp.322-329
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    • 2017
  • As a result of contemporary environmental concerns, a number of studies from plants' tissues as one of the alternatives to conventional chemicals are increasingly investigated. In tandem with these trends, Lagenaria breviflora (LB) fruit, reputed as antiviral and depilatory agents in the Yoruba folkloric medicine was examined on Vitex doniana wood to ascertain its antifungal activity. Fungicides of 25%, 50%, 75%, and 100% LB fruits formulations (concentrations) were developed through simple one-step mechanical-forming process, including control. In this study, the yield, the chemical compositions, the absorption capacity of the fungicides and wood weight losses (WWL) analysis were evaluated to investigate the antifungal activity of LB fruit on wood. The fruit extract yielded 35.4% of fresh juice weight. LB fruits contained total: alkaloids ($8.78{\pm}0.21mg/mL$), flavonoids ($2.01{\pm}0.02mg/mL$), phenol ($7.42{\pm}0.09mg/mL$), saponins ($11.00{\pm}0.10mg/mL$) and tannins ($5.47{\pm}0.05mg/mL$) contents. All the formulations provided effective protection against the tested wood fungi compared to control. Interestingly, the antifungal activity of 50% and 25% formulations of 6.8% WWL and 9.9% WWL satisfied the excellent fungal resistance class description against white rot fungus (Ganoderma lucidum) and brown rot fungus (Fibroporia vaillantii), respectively according to ASTM D 2017. These results thus, support LB fruit as a strong potential source of natural antifungals for industrial wood production.

자해 증상을 가진 발달장애아의 약물치료 (DRUG THERAPY FOR DEVELOPMENTAL DISORDER PATIENTS WITH SELF-MUTILATION)

  • 김봉석
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제14권1호
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    • pp.53-63
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    • 2003
  • 자폐장애는 Leo Kanner가 처음 기술한 이래, 독립된 임상적 실체로 인식되어 왔다. 자폐장애의 원인은 불분명하며, 여러 가지 뇌구조와 기능 이상을 보인다. 자해는 저기능 자폐증 환자에서 나타나며, 만성화되지 않도록 조기 치료가 필요하다. 본 논문은 자폐증의 여러 증상에 사용되는 약물을 고찰하였으며, 특히 자해와 공격성에 대한 약물을 중점으로 고찰하였다. 세로토닌 재흡수차단제는 일차적 항공격 효과가 불분명하다. 도파민 차단약물은 공격성과 자기 손상을 감소시키는데 상당한 효과를 보이는데 부작용의 측면에서 리스페리돈이 가장 유효하다. 날트렉손은 아직까지 일관된 연구결과가 나오지 않았다. 클로니딘은 공격성 감소의 효과가 있다. 또한 리튬이 공격적 자기손상적 행동의 치료에 효과적이었다. 그리고 카바마제핀 등의 항경련제가 공격적 폭발적 행동에 효과적이었다. 앞으로 단일한 진단과 치료반응 측정을 통해 자해에 대한 약물치료가 보다 진보할 것이다.

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