• Biotechnology and Bioprocess Engineering:BBE
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• 제5권5호
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• pp.332-339
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• 2000

Experimental and theoretical works were performed for the separation of large polyelectrolytes such as DNA in the column packed with gel particles under an electric field. This paper shows how intraparticle convection effects the separation of DNAs in the column because DNAs quickly oriented through the pores in the field direction. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria is theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified uing two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe(sub)f/Pe(sub)g of individual DNA. Governing equations are simultaneously solved by operator theoretic and characteristic methods to yield the column response.

• Biotechnology and Bioprocess Engineering:BBE
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• 제8권2호
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• pp.117-125
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• 2003

Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

• 전기화학회지
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• 제1권1호
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• pp.18-23
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• 1998

전기장이 충전층내 거대 이온성분자체의 분리를 위한 이론적 인 모델이 수행되었다 충전층내 여과재에서 전기적인 대류속도의 효과는 분자량이 큰 이온성 물질을 분리하기 위해서는 효과적이다. 이유는 전기장하에 거대이온성 분자체는 전기의 방향으로 빠르게 이온성분자체가 곧게 뻗는 현상때문이다. 이러한 분자체의 거동현상은 여과재내에 대류속도가 전기힘과 분자크기에 따라 대류속도가 다르기 나타나기 때문에 이들에 대한 연구는 충전층내에서 분자체를 분리하는데 도움이 된다 본 논문에서 여과재시스템내에서 이론분리는 확산에 대한 대류속도의 비$(Pe_t/Pe_g)$를 이용하여 예측하였으며 본 논문에서 얻어진 결과는 여과시스템을 설계하고 응용하는데 유효하게 적용될 것이며 이론적인 근거는 연산자 이론과 Reptation이론이 적용되였다.

• 한국응용과학기술학회지
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• 제18권1호
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• pp.1-6
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• 2001

Theoretical model has been studied for the transport phenomena of molecules in the system where an electric potential is applied to the system in the axial direction. The effect of electrophoretic convection in the polymeric media is significantly contributed to separate large ionic-molecules because the conformation of large ionic-molecule quickly orients in the field direction. The dependence of the transport in the polymeric media upon field intensity and molecular size aids in understanding the transport of large ionic-molecule in the system, since the convective velocity of large ionic-molecule is accelerated inside a porous material. The transport distance of individual large ionic-molecule can be predicted using the reptation theories.