• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.5
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• pp.332-339
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• 2000

Experimental and theoretical works were performed for the separation of large polyelectrolytes such as DNA in the column packed with gel particles under an electric field. This paper shows how intraparticle convection effects the separation of DNAs in the column because DNAs quickly oriented through the pores in the field direction. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria is theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified uing two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe(sub)f/Pe(sub)g of individual DNA. Governing equations are simultaneously solved by operator theoretic and characteristic methods to yield the column response.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.2
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• pp.117-125
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• 2003

Experimental and theoretical works were performed for the separation of large polyelectrolyte, such as DNA, in a column packed with gel particles under the influence of an electric field. Since DNA quickly orient in the field direction through the pores, this paper presents how intraparticle convection affects the residence time distribution of DNAs in the column. The concept is further illustrated with examples from solid -liquid systems, for example, from chromatography Showing how the column efficiency is improved by the use of a n electric field. Dimensionless transient mass balance equations were derived, taking into consideration both diffusion and electrophoretic convection. The separation criteria are theoretically studied using two different Peclet numbers in the fluid and solid phases. These criteria were experimentally verified using two different DNAs via electrophoretic mobility measurements. which showed how the separation position of the DNAs varies in the column in relation to the Peg/Pef values of an individual DNA. The residence time distribution was solved by an operator theory and the characteristic method to yield the column response.

• Journal of the Korean Electrochemical Society
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• v.1 no.1
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• pp.18-23
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• 1998

Theoretical model has been derived in the electrophoretic separation system where an electric potential is applied to the system in the axial direction. The effect of electrophoretic convection in the polymeric media is significantly contributed to separate large ionic-molecules because the conformation of large ionic-molecule quickly orients in the field direction. The dependence of the transport in the polymeric media upon field intensity and molecular size aids in understanding the transport of large ionic-molecule in the system, since the convective velocity of large ionic-molecule is accelerated inside a porous material. The separation of two different large ionic-molecules is predicted with a value of $(Pe_t/Pe_g)$ of individual large ionic-molecule using an operator and the reptation theories.

• Journal of the Korean Applied Science and Technology
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• v.18 no.1
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• pp.1-6
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• 2001

Theoretical model has been studied for the transport phenomena of molecules in the system where an electric potential is applied to the system in the axial direction. The effect of electrophoretic convection in the polymeric media is significantly contributed to separate large ionic-molecules because the conformation of large ionic-molecule quickly orients in the field direction. The dependence of the transport in the polymeric media upon field intensity and molecular size aids in understanding the transport of large ionic-molecule in the system, since the convective velocity of large ionic-molecule is accelerated inside a porous material. The transport distance of individual large ionic-molecule can be predicted using the reptation theories.