• 제목/요약/키워드: control programme

검색결과 133건 처리시간 0.027초

Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities

  • Fu, Bo;Ren, Liang;Liu, Di;Ma, Jian-Zhang;An, Tie-Zhu;Yang, Xiu-Qin;Ma, Hong;Zhang, Dong-Jie;Guo, Zhen-Hua;Guo, Yun-Yun;Zhu, Meng;Bai, Jing
    • Asian-Australasian Journal of Animal Sciences
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    • 제28권12호
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    • pp.1703-1712
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    • 2015
  • The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogenase (G6PDH) activity have shown superior quality than BCB negative (-) oocytes with high G6PDH activity, the use of a BCB staining test before IVM is still controversial. This study aimed to shed more light on the subcellular characteristics of porcine oocytes after selection using BCB staining. We assessed germinal vesicle chromatin configuration, cortical granule (CG) migration, mitochondrial distribution, the levels of acetylated lysine 9 of histone H3 (AcH3K9) and nuclear apoptosis features to investigate the correlation between G6PDH activity and these developmentally related features. A pattern of chromatin surrounding the nucleoli was seen in 53.0% of BCB+ oocytes and 77.6% of BCB+ oocytes showed peripherally distributed CGs. After IVM, 48.7% of BCB+ oocytes had a diffused mitochondrial distribution pattern. However, there were no significant differences in the levels of AcH3K9 in the nuclei of blastocysts derived from BCB+ and BCB- oocytes; at the same time, we observed a similar incidence of apoptosis in the BCB+ and control groups. Although this study indicated that G6PDH activity in porcine oocytes was correlated with several subcellular characteristics such as germinal vesicle chromatin configuration, CG migration and mitochondrial distribution, other features such as AcH3K9 level and nuclear apoptotic features were not associated with G6PDH activity and did not validate the BCB staining test. In using this test for selecting porcine oocytes, subcellular characteristics such as the AcH3K9 level and apoptotic nuclear features should also be considered. Adding histone deacetylase inhibitors or apoptosis inhibitors into the culture medium used might improve the efficiency of IVM of BCB+ oocytes.

초등학교 비만아동을 위한 비만관리프로그램이 혈중지질농도 변화에 미치는 효과 (The Effects of Weight Reduction Programme for Obese Primary School Students on Serum Lipid Level)

  • 정미희;한상숙;임소희
    • 한국산학기술학회논문지
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    • 제16권12호
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    • pp.8437-8446
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    • 2015
  • 본 연구는 초등학교 5~6학년 비만아동에게 식이요법, 운동요법 및 행동수정요법을 포함한 비만관리프로그램을 주 1회씩 12회에 걸쳐 시행한 실험군과 줄넘기 운동만을 적용한 대조군을 비교 확인하는 비동등성 대조군 전후시차 설계 연구이다. 효과를 확인하기 위해 혈중지질농도를 측정하였으며, 수집된 자료는 SPSS Windows 18.0 Program을 이용하여 ${\chi}^2$-test, t-test, ANOVA, Pairwise Comparisons으로 분석하였다. 비만관리프로그램은 총콜레스테롤은 대조군(m=40.7)이 실험군(m=16.95)에 비해 더 큰폭으로 증가하였으며(F=4.21, p=.049), 중성지방은 실험군이 $21.55mg/d{\ell}$가 감소하였으나, 대조군은 $37.45mg/d{\ell}$가 증가하였다(F=4.25, p=.049). 본 프로그램을 기초로 수정 보완하여 활용한다면 학교건강증진사업의 일환으로서 실효성을 거둘 수 있을 것으로 기대한다.

Analysis of Kinetic Data of Pectinases with Substrate Inhibition

  • Gummadi, Sathyanarayana-N.;Panda, T.
    • Journal of Microbiology and Biotechnology
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    • 제13권3호
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    • pp.332-337
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    • 2003
  • Enzyme kinetics data play a vital role in the design of reactors and control of processes. In the present study, kinetic studies on pectinases were carried out. Partially purified polymethylgalacturonase (PMG) and polygalacturonase (PG) were the two pectinases studied. The plot of initial rate vs. initial substrate concentration did not follow the conventional Michaelis-Menten kinetics, but substrate inhibition was observed. For PMG, maximum rate was attained at an initial pectin concentration of 3 g/l, whereas maximum rate was attained when the initial substrate concentration of 2.5 g/l of polygalacturonic acid for PG I and PG II. The kinetic data were fitted to five different kinetic models to explain the substrate inhibition effect. Among the five models tested, the combined mechanism of protective diffusion limitation of both high and inhibitory substrate concentrations (semi-empirical model) explained the inhibition data with 96-99% confidence interval.

한국 담배규제 정책의 평가: 담배규제정책 전문가 의견 조사를 토대로 (Evaluation of Tobacco Control Policy in Korea: Development and Application of the Korean Tobacco Index for Policy Monitoring and Evaluation)

  • 황지은;오유미
    • 보건행정학회지
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    • 제24권4호
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    • pp.342-348
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    • 2014
  • Background: Due to lack of regular and systematic evaluation tool, Korea's tobacco control policy has not been examined its overall process of implementation including efficiency and adequacy of the policies. This study developed policy monitoring and evaluation model to assess policy implementation and effectiveness of tobacco control in Korea. Methods: Based on World Health Organization operational manual for assessment, MPOWER (monitor tobacco use and prevention policies, protect from tobacco smoke, offer help to quit tobacco use, warn about the dangers of tobacco, enforce bans on tobacco advertising promotion and sponsorship, and raise taxes on tobacco) related policies were reviewed by rating policy efforts, programme management, people (human resources and their development), provision of organization, provision of fund and partnerships (range, 0 to 5). Results: As a result of the experts' assessment, overall Korean tobacco control policies scored 2.61 points, which is poor. In relation to each 'MPOWER' policies, 'W' scored the highest points (2.93), followed by 'O' (2.91), 'M' (2.87), 'P' (2.86), and 'E' (2.23). 'R' scored the lowest points of 1.87, meaning government efforts in tobacco price policy is insufficient. Conclusion: This study concludes that Korean tobacco control policy should strengthen tax and price measures, while programme infrastructure, people, and funds for policy enforcement should be secured. Furthermore, rather than focusing on one specific measure, a balanced approach reflecting various aspects of tobacco controls should be considered in order to decrease smoking rates and prevent smoking initiation.

중학교 "환경" 교과의 교수.학습 및 평가 방법 연구 (A Study on Teaching-Learning and Evaluation Methods of Environmental Studies in the Middle School)

  • 남상준
    • 한국환경교육학회지:환경교육
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    • 제7권1호
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    • pp.1-17
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    • 1994
  • This study was performed to determine appropriate teaching-learning and evaluation methods for Environmental Studies. To promote the relevance of our study to the needs of the schools and concerned educational communities of environmental education, we reviewed related literature, conducted questionnaire surveys, interviewed related teachers and administrator, held meetings with experts, and field-tested our findings. For selecting and developing teaching-learning methods of Environmental Studies, findings of educational research in general are considered. moreover, principles of environmental education, general aim of environmental education, orientations of environmental education, and developmental stages of middle school students in educational psychology were attended. In addition, relevance to the purpose of the Environmental Studies curriculum, appropriateness for value inquiry as well as knowledge inquiry, small group centered class organization, social interaction centered teaching-learning process, regional environmental situation, significance of personal environment, evaluation methods of Environmental Studies, multi- and inter-disciplinary contents of the Environmental Studies textbook, suitability to the evaluation methods of Environmental Studies, and emphasis on the social interaction in teaching-learning process were regarded. It was learned the Environmental Studies can be taught most effectively in via of holding discussion sessions, conducting actual investigation, doing experiment-practice, doing games and plate, role-playing and carrying out simulation activities, and doing inquiry. These teaching-learning methods were field-tested and proved appropriate methods for the subject. For selecting and developing evaluation method of Environmental Studies, such principles and characteristics of Environmental Studies as objective domains stated in the Environmental Studies curriculum, diversity of teaching-learning organization, were appreciated. We categorized nine evaluation methods: the teacher may conduct questionnaire surveys, testings, interviews, non-participatory observations; they may evaluate student's experiment-practice performances, reports preparation ability, ability to establish a research project, the teacher may ask the students to conduct a self-evaluation, or reciprocal evaluation. To maximize the effect of these methods, we further developed an application system. It considered three variables, that is, evaluates, evaluation objectives domains, and evaluation agent, and showed how to choose the most appropriate methods and, when necessary, how to combine uses of different methods depending on these variables. A sample evaluation instrument made on the basis of this application system was developed and tested in the classes. The system proved effective. Pilot applications of the teaching-learning methods and evaluation method were made simultaneously; and the results and their implications are as follows. Discussion program was applied in a lesson dealing with the problems of waste disposal, in which students showed active participation and creative thinking. The evaluation method used in this lesson was a multiple-choice written test for knowledge and skills. It was shown that this evaluation method and device are effective in helping students' revision of the lesson and in stimulating their creative interpretations and responces. Pupils showed great interests in the actual investigation program, and this programme was proved to be effective in enhancing students' participation. However, it was also turned out that there must be pre-arranged plans for the objects, contents and procedures of survey if this program is to effective. In this lesson, non-participatory observation methods were used with a focus on the attitudes of students. A scaled reported in general description rather than in grade. Experiment-practice programme was adopted in a lesson for purifying contaminated water and in this lesson, instruction objectives were properly established, the teaching-learning process was clearly specified and students were highly motivated. On the other hand, however, it was difficult to control the class when some groups of students require more times to complete their experiment, and sometimes different results. As regards to evaluation, performance observation test were used for assessing skills and attitudes. If teachers use well-prepared Likert scale, evaluation of all groups within a reasonablely short period of time will be possible. The most effective and successful programme in therms of students' participation and enjoyment, was the 'ah-nah-bah-dah-market' program, which is kind of game of the flea market. For better organized program of this kind, however, are essential, In this program, students appraise their own attitudes and behavior by responding to a written questionnaire. In addition, students were asked to record any anecdotes relating to self-appraisal of changes on one's own attitudes and behaviours. Even after the lesson, students keep recording those changes on letters to herself. Role-playing and simulation game programme was applied to a case of 'NIMBY', in which students should decide where to located a refuse dumping ground. For this kind of programme to e successful, concepts and words used in the script should be appropriate for students' intellectual levels, and students should by adequately introduced into the objective and the procedures of the lessons. Written questionnaire was used to assess individual students' attitudes after the lesson, but in order to acquire information on the changes of students' attitudes and skills, pre-test may have to be made. Doing inquiry programme, in which advantages in which students actually investigated the environmental influence of the areas where school os located, had advantages in developing students' ability to study the environmental problems and to present the results of their studies. For this programme to be more efficient, areas of investigation should be clearly divided and alloted to each group so that repetition or overlap in areas of study and presentation be avoided, and complementary wok between groups bee enhanced. In this programme, teacher assessed students' knowledge and attitudes on the basis of reports prepared by each group. However, there were found some difficults in assessing students' attitudes and behaviours solely on the grounds of written report. Perhaps, using a scaled checklist assessing students' attitudes while their presentation could help to relieve the difficulties.

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출산력 억제정책의 영향과 변천에 관한 고찰 (Change in the Korean Fertility Control Policy and its Effect)

  • 홍문식
    • 한국인구학
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    • 제21권2호
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    • pp.182-227
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    • 1998
  • 정부는 1960년대 초기의 높은 인구증가율이 경제개발의 저해 요인임을 인식하여 1962년부터 시작되는 5개년 단우의 경제개발 장기계획의 일환으로 가족계획사업을 출산조절정책 수단으로 수용하였다. 초창기부터 가족계획사업은 정부의 보건조직망을 통하여 가족계획요원에 의한 피임보급 활동과 지정시술의사에 의한 자궁내장치 및 불임시술 서비스 등이 무료로 제공되었고 특히 피임방법별 목표량 제도와 규제 및 보상 등 사회제도적 지원시책으로 1980년대까지 지속적으로 사업이 강화되었다. 민간단체의 지원활동으로는 대한가족계획협회에 의한 계몽교육사업과 한국 보건사회연구원(초창기에는 가족계획연구원)에 의한 사업평가 및 조사연구사업이 활발히 이루어 졌다. 결과적으로 1960년에 6명 수준이던 합계출산율이 1980년대 중반에 대치수준(2.1)으로 저하되어 30년도 못되는 단기간에 인구전환을 이룩하는 획기적인 성과를 갖게 되었다. 그후 합계출산율은 1.6에서 1.7 범위의 저출산을 유지하고 있으며 이러한 수준이 지속된다면 현 1% 미만인 인구성장률은 2028년에 총인구가 5,060만 수준에서 그 성장을 멈추고 그 후로는 인구의 감소가 초래될 것으로 예상된다. 이에 정부는 1996년 6월에 기존의 인구억제정책을 전면적으로 폐지하고 인구자질향상에 역점을 두는 방향으로 공식적인 정책전환을 이룩하였다. 한편 남아선호사상 등 영향으로 태아의 성감별에 의한 성선별적 인공임신중절로 인하여 출생성비의 불균형이 심화되고 유배우 부인의 인공임신중절도 아직 상당수준으로 높게 지속되고 있어 삶의질 차원에서의 새로운 인구자질향상 정책이 특히 모자보건과 노인보건을 포함하는 전반적인 국민건강증진 프로그램과 함께 더욱 효과적으로 추진되어 복지사회 구현에 기여할 수 있는 사업으로 발전되는 것이 바람직할 것이다.

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Association of CYP2E1, STK15 and XRCC1 Polymorphisms with Risk of Breast Cancer in Malaysian Women

  • Chong, Eric Tzyy Jiann;Goh, Lucky Poh Wah;See, Edwin Un Hean;Chuah, Jitt Aun;Chua, Kek Heng;Lee, Ping-Chin
    • Asian Pacific Journal of Cancer Prevention
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    • 제17권2호
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    • pp.647-653
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    • 2016
  • Background: Breast cancer is the most common type of cancer affecting Malaysian women. Recent statistics revealed that the cumulative probability of breast cancer and related deaths in Malaysia is higher than in most of the countries of Southeast Asia. Single nucleotide polymorphisms (SNPs) in CYP2E1 (rs6413432 and rs3813867), STK15 (rs2273535 and rs1047972) and XRCC1 (rs1799782 and rs25487) have been associated with breast cancer risk in a meta-analysis but any link in Southeast Asia, including Malaysia, remained to be determined. Hence, we investigated the relationship between these SNPs and breast cancer risk among Malaysian women in the present case-control study. Materials and Methods: Genomic DNA was isolated from peripheral blood of 71 breast cancer patients and 260 healthy controls and subjected to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Results: Our study showed that the c1/c2 genotype or subjects with at least one c2 allele in CYP2E1 rs3813867 SNP had significantly increased almost 1.8-fold higher breast cancer risk in Malaysian women overall. In addition, the variant Phe allele in STK15 rs2273535 SNP appeared to protect against breast cancer in Malaysian Chinese. No significance association was found between XRCC1 SNPs and breast cancer risk in the population. Conclusions: This study provides additional knowledge on CYP2E1, STK15 and XRCC1 SNP impact of risk of breast cancer, particularly in the Malaysian population. From our findings, we also recommend Malaysian women to perform breast cancer screening before 50 years of age.

Modeling and fast output sampling feedback control of a smart Timoshenko cantilever beam

  • Manjunath, T. C.;Bandyopadhyay, B.
    • Smart Structures and Systems
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    • 제1권3호
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    • pp.283-308
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    • 2005
  • This paper features about the modeling and design of a fast output sampling feedback controller for a smart Timoshenko beam system for a SISO case by considering the first 3 vibratory modes. The beam structure is modeled in state space form using FEM technique and the Timoshenko beam theory by dividing the beam into 4 finite elements and placing the piezoelectric sensor/actuator at one location as a collocated pair, i.e., as surface mounted sensor/actuator, say, at FE position 2. State space models are developed for various aspect ratios by considering the shear effects and the axial displacements. The effects of changing the aspect ratio on the master structure is observed and the performance of the designed FOS controller on the beam system is evaluated for vibration control.

비례압력제어밸브의 부하압력 피드백방법 개선에 관한 연구 (A Study on the Improvement of the Load Pressure Feedback Mechanism of the Proportional Pressure Control Valve)

  • 오인호;장지성;이일영;정대종;조성현
    • 대한기계학회논문집A
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    • 제21권5호
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    • pp.719-726
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    • 1997
  • The proportional pressure control valve having versatile functions and higher performance is an essential conponent in the open loop controlled rear wheel steering gear of the four wheel steering system in a passenger car. In this study, the authors suggest a new type of load pressure feedback mechanism which can make it easy to change the range of controlled pressure without changing the capacity of solenoid. The concept of suggested mechanism, composed of the pressure chamber with throttles in series, was described. The mathematical model was derived from the rear wheel steering gear consisting of a valve and a cylinder for the purpose of analyzing the valve characteristics. And the programme for computing the characteristic of the valve was developed. Experiments were performed to confirm the performance of the valve and computations were carried out to ascertain the usefulness of the developed programme. The results from computations fairly coincide with those from experiments. And the results from experiments and computations show that the performance of new valve was as good as that of the already developed one and the new valve has advantages such as the easiness of changing the range of controlled pressure and the decrease of power loss at neutral position without the decline of performance.

탄력저항을 이용한 하지근력 강화 운동이 여성노인의 정적 및 동적 균형능력에 미치는 영향 (Effects of Lower Extremity Muscle Strengthening Exercise Using Elastic Resistance on Balance on Elderly Women)

  • 이한주;한상완
    • 지역사회간호학회지
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    • 제20권1호
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    • pp.59-66
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    • 2009
  • Purpose: The purpose of study was to test the effect of lower extremity muscle strengthening exercise using elastic resistance on balance on elderly women. Methods: Twenty four women who were over 65 years old were randomly assigned to either the experimental group that received the exercise programme (n=12) or to the control group (n=12) that continued normal activities except programme. Exercises included the following 7 different activities; chair squat, knee flection, extension, hip flection, extension, calf muscle raise, ankle dorsiflection. All the participants were subject to 3 tests, including One-Leg Standing Test, Functional Reaching Test, and Up & Go Test. The measurements were made before the excercise, 4 and 8 weeks after the exercise. The data were analyzed by using the two way repeated ANOVA. Results: The results were as follows; As compared with change of balance capacity between groups, a significant difference was shown in the up and go test (p<.05), but not in balance performance clinical test of OLST (p>.05) and FRT (p>.05). Conclusion: The lower extremity muscle strengthening exercise using elastic resistance for the elderly women had some positive effects on the balance. Therefore this intervention can be broadly applied to other elders for preventing falls.

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