• Title/Summary/Keyword: contrast resolution

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Utilization of Light Microscopy and FFT for MFA Measurement from Unstained Sections of Red Pine (Pinus Densiflora)

  • Kwon, Ohkyung;Lee, Mi-Rim;Eom, Chang-Deuk
    • Journal of the Korean Wood Science and Technology
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    • v.41 no.5
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    • pp.399-405
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    • 2013
  • This study demonstrates the utilization of light microscopy and Fast Fourier Transform-Peak Finding (FPF) method for microfibril angle (MFA) measurement from unstained sections of red pine (Pinus densiflora). To obtain an image with optimal contrast and resolution for MFA measurement, effects of numerical aperture (NA) of condenser lens and color filters were investigated. About 60% of NA of the maximum condenser NA produced an image with optimal contrast, but a color filter with short wavelength range (DAPI) created images with improved resolution. Manual angle measurement and the FPF method were applied to the image with optimal contrast for MFA measurement. The experimental results from the FPF method were considered to be more repeatable and less subjective than those from the manual angle measurement.

Crystallographic Characterization of the (Bi, La)4Ti3O12 Film by High-Resolution Electron Microscopy (고분해능 전자현미경법을 이용한 (Bi, La)4Ti3O12 박막의 결정학적 특성 평가)

  • Lee, Doek-Won;Yang, Jun-Mo;Park, Tae-Su;Kim, Nam-Kyung;Yeom, Seung-Jin;Park, Ju-Chul;Lee, Soun-Young;Park, Sung-Wook
    • Korean Journal of Materials Research
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    • v.13 no.7
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    • pp.478-483
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    • 2003
  • The crystallographic characteristics of the $(Bi, La)_4$$Ti_3$$O_{12}$ thin film, which is considered as an applicable dielectrics in the ferroelectric RAM device due to a low crystallization temperature and a good fatigue property, were investigated at the atomic scale by high resolution transmission electron microscopy and the high resolution Z-contrast technique. The analysis showed that a (00c) preferred orientation and a crystallization of the film were enhanced with the diffraction intensity increase of the (006) and (008) plane as the annealing temperature increased. It indicated a change of the atomic arrangement in the (00c) plane. Stacking faults on the (00c) plane were also observed. Through the comparison of the high-resolution Z-contrast image and the $Bi_4$$Ti_3$$O_{12}$ atomic model, it was evaluated that the intensity of the Bi atom was different according to the atomic plane, and it was attributed to a substitution of La atom for Bi at the specific atom position.

Dose and Image Assessment according to Radiologic Factors Variation at Digital Humerus X-ray Examination (디지털 환경에서 Humerus 검사 시 촬영인자 변화에 따른 선량 및 화질 평가)

  • Kim, Seong Min;Hong, Seon Sook;Lee, Kwan Sup;Ha, Dong Yun
    • Korean Journal of Digital Imaging in Medicine
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    • v.14 no.2
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    • pp.1-8
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    • 2012
  • Purpose : We aim at presenting the optimum radiologic factor through the evaluation of dose variation and of image quality through the use of a grid in Humerus examination and the change of dose because of the change of radiologic factor. Materials and Methods : We divided it in 3 cases: when using a grid or not and when using IP(Image Plate) in a digital system. Also, as fixing kVp to 70kVp it changed mAs, and fixing mAs to 10 it changed kVp, we put up resolution chart and Burger rose phantom on the acrylic phantom of 7cm (the same level of Humerus) to evaluate the dose and image. We used Image J program to evaluate the quantitative resolution of the obtained image, and made the qualitative evaluation and statistical analysis of the image saved in PACS for 20 radiologic technologist with more than 10 years of experience in order of evaluate its contrast. We used SPSS10(SPSS Inc. Chicago, Illinois) for statistical analysis. Results : We observed the analytic result of resolution by the change of kVp that it was $4.539dGycm^2$ in 60kVp and $757.472dGycm^2$ in 75kVp, which increased about 64.6% of dose, while for the resolution it had the pixel value 30.7% better with 851 in 60kVp than 651 in 75kVp. Also, we analyzed the result of resolution by the change of mAs that it was $3.106dGycm^2$ in 5mAs, and $12.470dGycm^2$ in 20mAs, which increased about 400% of dose, while for the resolution DR had 678 in 5mAs, and 724 in 20mAs that increased about 6.8% of resolution. We made the qualitative evaluation of contrast by the change of kVp that DR showed the higher quality than CR, but the contrast by the change of kVp had no special different at the moment of visual evaluation, nor statistically significant difference(P>0.05). We observed the qualitative evaluation of contraste by the change of mAs that the contrast increased as DR increased mAs, and had statistically significant difference(P<0.05). On the other hand, CR had no significant difference for more than 10mAs nor statistically significant difference(P>0.05). Conclusion : In case of some patients with radiographic exposure by the repeated examination such as emergent patient or Follow up patient, they are considered to try to limit the use of a grid, to set kVp under 65kVp in fixed mode, to select less than 10mAs and to reduce the possibility of patient being bombed.

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Biological Applications of Helium Ion Microscopy

  • Kim, Ki Woo
    • Applied Microscopy
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    • v.43 no.1
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    • pp.9-13
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    • 2013
  • The helium ion microscope (HIM) has recently emerged as a novel tool for imaging and analysis. Based on a bright ion source and small probe, the HIM offers advantages over the conventional field emission scanning electron microscope. The key features of the HIM include (1) high resolution (ca. 0.25 nm), (2) great surface sensitivity, (3) great contrast, (4) large depth-of-field, (5) efficient charge control, (6) reduced specimen damage, and (7) nanomachining capability. Due to the charge neutralization by flood electron beam, there is no need for conductive metal coating for the observation of insulating biological specimens by HIM. There is growing evidence that the HIM has substantial potential for high-resolution imaging of uncoated insulating biological specimens at the nanoscale.

A Study on the Optimal Design for the reconstruction Filter in Single Photon Emission Computed Tomography (SPECT) (단일광자방출 전산화 단층촬영상에서 재구성 필터의 최적설계에 관한 연구)

  • 김정희;김광익
    • Journal of Biomedical Engineering Research
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    • v.18 no.2
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    • pp.113-120
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    • 1997
  • This paper presents an optimal design for the SPECT reconstruction filter, based on a physical limit of SPECT lesion detection capability. To increase the performance of the filter on lesion detectability, the filter design was focused on increasing the local SyW ratio of a threshold lesion, that was determined by minimum detectable lesion size (MDU) from SPECT lesion detectabllity contrast-detail curve. The proposed filter showed flexible window characteristics of resolution recovery and noise smoothing for MDLSs in the resolution-limited and photon-limited regions, respectively, compennting for the relative impact of the main limitation factors on threshold detectability. The simulated results showed good adaptability of the proposed filter to the changes in physical parameters of photon counts, object contrast, and detector system resolution.

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Evaluation of Contrast and Resolution on the SPECT of Pre and Post Scatter Correction (산란보정 전, 후의 SPECT 대조도 및 분해능 평가)

  • Seo, Myeong-Deok;Kim, Yeong-Seon;Jeong, Yo-Cheon;Lee, Wan-Kyu;Song, Jae-Beom
    • The Korean Journal of Nuclear Medicine Technology
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    • v.14 no.1
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    • pp.127-132
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    • 2010
  • Purpose: Because of limitation of image acquisition method and acquisition time, scatter correction cannot perform easily in SPECT study. But in our hospital, could provide to clinic doctor of scatter corrected images, through introduction of new generation gamma camera has function of simple scatter correction. Taking this opportunity, we will compare scatter corrected and non-scatter corrected image from image quality of point of view. Materials and Methods: We acquisite the 'Hoffman brain phantom' SPECT image and '1mm line phantom' SPECT image, each 18 times, with GE Infinia Hawkeye 4, SPECT-CT gamma camera. At first, we calculated each contrast from axial slice of scatter corrected and non-scatter corrected SPECT image of 'Hoffman brain phantom'. and next, calculated each FWHM of horizontal and vertical from axial slice of scatter corrected and non-scatter corrected SPECT image of '1mm line phantom'. After then, we attempted T test analysis with SAS program on data, contrast and resolution value of scatter corrected and non-scatter corrected image. Results: The contrast of scatter corrected image, elevated from 0.3979 to 0.3509. And the resolution of scatter corrected image, elevated from 3.4822 to 3.6375. p value were 0.0097 in contrast and <0.0001 in resolution. We knew the fact that do improve of contrast and resolution through scatter correction. Conclusion: We got the improved SPECT image through simple and easy way, scatter correct. We will expect to provide improved images, from contrast and resolution point of view. to our clinic doctor.

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The Design and Implementation of Real Time Contrast Enhancer System for High Resolution FPD (고해상도 FPD를 위한 실시간 Contrast Enhancer System의 설계 및 구현)

  • Seo, Bum-Suk;Choi, Chul-Ho;Kwon, Byeong-Heon
    • Journal of Digital Contents Society
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    • v.5 no.1
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    • pp.79-86
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    • 2004
  • In this paper we implemented the Real Time Contrast Enhancer for image quality enhancement of moving picture. Also we proposed adaptive contrast method that use mean and variance of input video signal. The Designed the contrast Enhancer is measured in comparison with conventional picture and interfaced to 30inch TFT LCD TV of the LG Electronics.

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The Comparison of the SIFT Image Descriptor by Contrast Enhancement Algorithms with Various Types of High-resolution Satellite Imagery

  • Choi, Jaw-Wan;Kim, Dae-Sung;Kim, Yong-Min;Han, Dong-Yeob;Kim, Yong-Il
    • Korean Journal of Remote Sensing
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    • v.26 no.3
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    • pp.325-333
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    • 2010
  • Image registration involves overlapping images of an identical region and assigning the data into one coordinate system. Image registration has proved important in remote sensing, enabling registered satellite imagery to be used in various applications such as image fusion, change detection and the generation of digital maps. The image descriptor, which extracts matching points from each image, is necessary for automatic registration of remotely sensed data. Using contrast enhancement algorithms such as histogram equalization and image stretching, the normalized data are applied to the image descriptor. Drawing on the different spectral characteristics of high resolution satellite imagery based on sensor type and acquisition date, the applied normalization method can be used to change the results of matching interest point descriptors. In this paper, the matching points by scale invariant feature transformation (SIFT) are extracted using various contrast enhancement algorithms and injection of Gaussian noise. The results of the extracted matching points are compared with the number of correct matching points and matching rates for each point.

Evaluation of PET Image for Fluorine-18 and Gallium-68 using Phantom in PET/CT (PET/CT에서 Phantom을 이용한 Fluorine-18, Gallium-68 방사성 핵종의 PET 영상 평가)

  • Yoon, Seok-Hwan
    • Journal of radiological science and technology
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    • v.41 no.4
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    • pp.321-327
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    • 2018
  • The purpose of this study is to compare PET imaging performance with Fluorine-18 ($^{18}F$) and Gallium-68 ($^{68}Ga$) for influence of physical properties of PET tracer. Measurement were performed on a Siemens Biograph mCT64 PET/CT scanner using NEMA IEC body phantom and Flangeless Esser PET phantom containing filled with $^{18}F$ and $^{68}Ga$. Emission scan duration(ESD) was set to 1, 2, 3, 4 and 5min/bed for $^{68}Ga$ and 1min/bed for $^{18}F$. The PET image were evaluated in terms of contrast, spatial resolution. Under same condition, The percentage of contrast recovery measured in the phantom ranged from 16.88% to 72.56% for $^{68}Ga$ and from 27.51% to 74.43% for $^{18}F$ and The FWHM value to evaluate spatial resolution was 10.96 mm for $^{68}Ga$ and 9.19 mm for $^{18}F$. For this study, $^{18}F$ produces better image contrast and spatial resolution than $^{68}Ga$ due to higher positron yield and lower positron energy ($^{18}F$: 96.86%, 633.5 keV, $^{68}Ga$: 88.9%, 1899 keV), The physical properties of PET tracer effect on the PET image. $^{68}Ga$ image applying ESD of 3, 4, 5min/bed were showed similar to $^{18}F$ image with ESD of 1min/bed. This study suggests that increasing ESD for acquiring $^{68}Ga$ PET image seem to be similar to $^{18}F$ image.

Application of a newly developed software program for image quality assessment in cone-beam computed tomography

  • de Oliveira, Marcus Vinicius Linhares;Santos, Antonio Carvalho;Paulo, Graciano;Campos, Paulo Sergio Flores;Santos, Joana
    • Imaging Science in Dentistry
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    • v.47 no.2
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    • pp.75-86
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    • 2017
  • Purpose: The purpose of this study was to apply a newly developed free software program, at low cost and with minimal time, to evaluate the quality of dental and maxillofacial cone-beam computed tomography (CBCT) images. Materials and Methods: A polymethyl methacrylate (PMMA) phantom, CQP-IFBA, was scanned in 3 CBCT units with 7 protocols. A macro program was developed, using the free software ImageJ, to automatically evaluate the image quality parameters. The image quality evaluation was based on 8 parameters: uniformity, the signal-to-noise ratio (SNR), noise, the contrast-to-noise ratio (CNR), spatial resolution, the artifact index, geometric accuracy, and low-contrast resolution. Results: The image uniformity and noise depended on the protocol that was applied. Regarding the CNR, high-density structures were more sensitive to the effect of scanning parameters. There were no significant differences between SNR and CNR in centered and peripheral objects. The geometric accuracy assessment showed that all the distance measurements were lower than the real values. Low-contrast resolution was influenced by the scanning parameters, and the 1-mm rod present in the phantom was not depicted in any of the 3 CBCT units. Smaller voxel sizes presented higher spatial resolution. There were no significant differences among the protocols regarding artifact presence. Conclusion: This software package provided a fast, low-cost, and feasible method for the evaluation of image quality parameters in CBCT.