• 한국양식학회지
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• 제16권1호
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• pp.8-14
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• 2003

To generate expressed sequence tags for genomics research involving genetic linkage analysis, to examine gene expression profiles in muscles of channel catfish in a non-normalized muscle cDNA library, to compare gene expression in young and mature channel catfish muscles using the EST reagents and gene filters to demonstrate the feasibility of functional genomics research in small laboratories. 102 randomly picked cDNA clones were analyzed from the catfish muscle cDNA library. Of the sequences generated, 90.2% of ESTs was identified as known genes by identity comparisons. These 92 clones of known gene products represent transcriptional products of 24 genes. The 10 clones of unknown gene products represent 8 genes. The major transcripts (70.1% of the analyzed ESTs) in the catfish muscle are from many major genes involved in muscle contraction, relaxation, energy metabolism and calcium binding such as alpha actin, creatine kinase, parvalbumin, myosin, troponins, and tropomyosins. Gene expression of the unique ESTs was comparatively studied in the young and adult catfish muscles. Significant differences were observed for aldolase, myostatin, myosin light chain, parvalbumin, and an unknown gene. While myosin light chain and an unknown gene (CM 192) are down-regulated in the mature fish muscle, the aldolase, myostatin, and parvalbumin are significantly up-regulated in the mature fish muscle. Although the physiological significance of the changes in expression levels needs to be further addressed, this research demonstrates the feasibility and power of functional genomics in channel catfish. Channel catfish muscle gene expression profiles provide a valuable molecular muscle physiology blueprint for functional comparative genomics.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.955-958
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• 2003

Nepal is a country of mountains The higher order geodetic points were mostly established on the top of mountains and these points were used for the geodetic network extension. Lower order geodetic control networks were established at different times and used for the surveying and mapping activities of the country.. It has been found that the rate of convergence between north and south borders of Nepal to be 21${\pm}$2 mm each year and the rate translation of Kathmandu to 55${\pm}$3 mm/year to the plates. The most intense deformation in Nepal occurs along the belt of high mountains along its northern border res ulting in a strain contraction rate normal to the Himalayan Arc. This belt is approximately 40 km wide and extends into southern Tibet.( 13). Recently Survey Department of Nepal has lunched a program of strengthening the existing geodetic network of Nepal and re-observed the position of higher order geodetic points by using geodetic GPS receivers to evaluate their position and thus to define the precision of the control points once again. This paper describes the observation procedure and the adjustment results of the existing higher order control network of Nepal established in different time using different types of equipment and techniques; and highlights the observation procedure and the result obtained after the post processing of the GPS observation results. Attempt has been made to give the procedure and identify the methodology for the re observation of existing higher order geodetic points by using GPS receiver and post processing the observed data so that the existing higher order geodetic points are within the given accuracy standard.