• Current Research on Agriculture and Life Sciences
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• 제30권2호
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• pp.68-75
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• 2012

본 연구에서는 항암효과가 뛰어난 차가버섯의 초음파 추출 공정 최적화를 위해 반응표면 분석법으로 모니터링하여 최적 추출 조건을 설정하였다. 중심합성계획법에 따라 추출 시간($X_1$), 에탄올 농도($X_2$), 추출 온도($X_3$)을 요인변수로 하고 추출 수율($Y_1$), 총 페놀함량($Y_2$), 총 플라보노이드함량($Y_3$), 갈색도($Y_4$)를 종속변수로 하여 시행 하였다. 실험 결과 추출 수율은 에탄올 농도와 추출 온도에 영향을 받음을 알 수 있었다. 안장점에서 추출 조건이 추출 시간 20.47min($X_1$), 에탄올 농도 42.85%($X_2$), 추출 온도 $69.57^{\circ}C$($X_3$)일 때, 최대값 18.02%로 나타났다. 총 페놀함량 또한 에탄올 농도와 추출 온도에 영향을 크게 받았다. 최대값은 71.57mg GAE/g으로 나타났으며 이때의 추출 조건은 추출시간 21.60min($X_1$), 에탄올 농도 45.19%($X_2$), 추출 온도 $69.68^{\circ}C$($X_3$)로 나타났다. 총 플라보노이드함량은 추출 온도의 영향을 받은 것으로 나타났고 안장점에서 추출 조건은 추출 시간 22.53min($X_1$), 에탄올 농도 46.37%($X_2$), 추출온도 $69.56^{\circ}C$($X_3$)였고 최대값은 35.98mg RE/g으로 예측되었다. 갈색도는 추출 시간, 에탄올 농도, 추출 온도 이 세가지 조건 모두에 영향을 크게 받았다. 안장점일 때의 추출시간 22.00min($X_1$), 에탄올 농도 46.89%($X_2$), 추출 온도 $69.71^{\circ}C$($X_3$)의 조건에서 최대값이 예측되었다. 앞에서 얻은 결과들을 바탕으로 contour map을 분석한 결과 추출시간 21.50min, 에탄올 농도 44.87%, 추출 온도 $69.64^{\circ}C$의 최적 추출 조건이 예측되었다.

• 산업식품공학
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• 제15권4호
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• pp.388-397
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• 2011

본 연구에서는 추출시간을 단축시키고자 Ultrasound-Assisted Extraction(UAE)를 이용한 전처리 추출공정을 수행 후 기능성 성분 추출 시 시료에 대한 용매비와 추출 온도, 추출 시간의 영향을 알아보기 위하여 용매비는 10-30 mL/g, 추출온도 60-100$^{\circ}C$, 추출시간 2-6시간으로 하여 각 조건에서 추출함량을 측정하였으며 최적 추출 조건을 구하기 위해 반응표면 분석을 실시하였다. 그 결과, 미강의 기능성 성분의 추출 시 추출온도에 가장 영향을 많이 받았으며 추출시간은 크게 영향을 받지 않았다. 시료에 대한 용매비는 추출온도 다음으로 종속변수에 비교적 유의하게 영향을 주었다. 총페놀함량의 추출조건은 시료에 대한 용매비 17.43, 추출온도 82.13$^{\circ}C$, 추출시간 3.76시간일 때 최대값 176.57 mg GAE/100g을 나타내었고, 총플라보노이드 함량은 18.32 mL/g, 77.51$^{\circ}C$, 2.04시간일 때 291.09 mg/ 100 g으로 최대값을 나타내었다. 전자공여능은 14.37 mL/g, 78.46$^{\circ}C$, 2.87시간일 때 94.34%로 최대값을 나타내었다. Ferulic acid의 추출조건은 시료에 대한 용매비 17.71 mL/g, 추출 온도 80.58$^{\circ}C$, 추출시간 3.62시간일 때 최대값 265.18 mg/100 g으로 예측되었고, $\gamma$-oryzanol은 시료에 대한 용매비 16.39 mL/g, 추출온도 76.45$^{\circ}C$, 추출시간 3.41시간 에서 최대값 226.46 mg/100 g을 나타났다. $\alpha$-toopherol은 시료에 대한 용매비 25 mL/g, 추출온도 79.18$^{\circ}C$, 추출 시간 5.73시간일 때 최대값 9.71 mg/100 g을 나타내었다. 흑미 미강의 기능성 성분의 최적 추출 조건을 예측하기 위하여 각 종속변수에 대한 각각의 contour map을 superimposing을 한 결과 시료에 대한 용매비 20.35mL/g, 추출 온도 79.4$^{\circ}C$, 추출 시간 2.88시간으로 나타났다. 이 조건에서 실험한 결과 각각 종속변수들의 예측값과 실제값이 유사하게 나타났다.

• 대한예방한의학회지
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• 제4권2호
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• pp.72-92
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• 2000

This research has conducted studies on an Oriental medicine-based method of diagnosing of occupational musculoskeletal system diseases. This researcher has searched through existing relevant medical literature. Also, this researcher has worked on a moire topography using moire topography. In this course, this researcher has reached the following conclusion in relation to the possibility of using a moire topography as a diagnosing device of musculoskeletal system diseases under Oriental medicine . 1 The Western medicine outlines its criteria of screening occupational musculoskeletal system diseases as follows A. The occupational musculoskeletal diseases must clearly include one or more of the subjective symptoms characterized by pain, hypoesthesia dysaesthesia, anaesthesia. etc . B, There should be clinically admitted objective observations and diagnosis outlining that the disease concerned shows symptoms such as tenderness, induration. and edema that can appear with occupational musculoskeletal system diseases. dyscinesia should be admitted with the disease concerned, or there should be observations and diagnosis outlining that abnormality exists in electric muscular or nervous diagnosis and examination . C. It should be admitted that prior to the occurrence of symptoms or observations and diagnosis on musculoskeletal system-related diseases, a patient has been engaged in works with conditions requiring improper work posture or work movement. That is, this is an approach whereby they see abnormality in the musculoskeletal system come from material and structural defect, and adjust and control abnormality in the musculoskeletal system and secreta . 2. The Oriental medicines sees that a patient develops the pain of occupational musculoskeletal diseases as he cannot properly activate the flow of his life force and blood thus not only causing formation of lumps in the body and blocking the flow of life force and blood in some parts of the body. Hence, The Oriental medicine focuses on resolving the cause of weakening the flow of life force and blood, instead of taking material approach of correcting structural abnormality Furthermore , Oriental medicine sees that when muscle tension builds up, this presses blood vessels and nerves passing by, triggering circulation dyscrasia and neurological reaction and thus leading to lesion. Thus, instead of taking skeletal or neurophysiological approach. it seeks to fundamentally resolve the cause of the flow of the life force and blood in muscles not being activated. As a result Oriental medicine attributes the main cause of musculoskeletal system diseases to muscle tension and its build-up that stem from an individual's long formed chronicle habit and work environment. This approach considers not only the social structure aspect including companies owners and work environment that the existing methods have looked at, but also individual workers' responsibility and their environmental factors. Hence, this is a step forward method. 3 The diagnosis of musculoskeletal diseases under Oriental medicine is characterized by the fact that an Oriental medicine doctor uses not only photos taken by himself, but also various detection devices to gather information and pass comprehensive judgment on it. Thus, it is the core of diagnosis under Oriental medicine to develop diagnosing devices matching the characteristics of information to be induced and to interpret information so induced from the views of Oriental medicine. Diagnosis using diagnosing devices values the whole state of a patient and formal abnormality alike, and the whole balance and muscular state of a patient serves as the basis of diagnosis. Hence, this method, instead of depending on the information gathered from devices under Western medicine, requires devices that provide information on the whole state of a patient in addition to the local abnormality information that X-ray. CT, etc., can offer. This method sees muscle as the central part of the abnormality in the musculoskeletal system and thus requires diagnosing devices enabling the muscular state. 4. The diagnosing device using moire topography under Oriental medicine has advantages below and can be used for diagnosing musculoskeletal system diseases with industrial workers . First, the device can Provide information on the body in an unbalanced state. and thus identify the imbalance and difference of height in the left and right stature that a patient can not notice at normal times. Second, the device shows the twisting of muscles or induration regions in a contour map. This is not possible with existing shooting machines such as X-ray, CT, etc., thus differentiating itself from existing machines. Third, this device makes it possible for Oriental medicine to take its unique approach to the abnormality in the musculoskeletal system. Oriental medicine sees the state and imbalance state in muscles as major factors in determining the lesion of musculoskeletal system, and the device makes it possible to shoot the state of muscles in detail. In this respect, the device is significant. Fourth, the device has an advantage as non-aggression diagnosing device.