• Title/Summary/Keyword: constitutive relationship

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Isolation and Characterization of High Viscosity Polysaccharide Producing Endophytic Bacteria from Pueraria Root (고점도 다당류를 생산하는 갈근 내생균의 분리 및 특성)

  • Whang, Kyung-Sook;Choi, Seung-Hyun;Han, Song-Ih
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.341-345
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    • 2007
  • Fifty endophytic bacteria, which produced slime around the colonies, were isolated from Pueraria roots. In particular, HDN-14, TDG-3, and TNB-3 strains, which appeared to be high viscosity producers, were selected. These strains produced high levels of polysaccharides in Puerara root medium extract. The purified polysaccharide was digested with 1N HCI and analyzed by HPLC, with glucose ($45.6{\sim}63.1%$), maltose ($14.6{\sim}23.7%$), and fructose ($17.4{\sim}23.7%$) detected as constitutive sugars. When determined by the homology relationship of the 16S rDNA sequence with the relative taxa, the HDN-14 and TNB-3 strains were closely ($99.06{\sim}99.32%$) related to the Pseudomonas $koreensis^T$ and Pseudomonas $jessenii^T$, while TDG-3 were closely ($99.48{\sim}99.74%$) related to Pseudomonas $plecoglossicida^T$, Pseudomonas $mosselii^T$, and Pseudomonas $monteilii^T$. The major cellular Pseudomonas acids are $3OH-C_{10:0}$, $2OH-C_{12:0}$, $3OH-C_{12:0}$, and $3OH-C_{12:1}$, with these strains being further differentiated in species belonging to the genus Pseudomonas.

Analysis of Tree-rings for Inference of Periods in which Slow-moving Landslides Occur (나이테 분석을 통한 땅밀림 발생 시기 추정)

  • Park, Jae-Hyeon;Park, Seonggyun
    • Journal of Korean Society of Forest Science
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    • v.109 no.1
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    • pp.62-71
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    • 2020
  • With the aim of restoring slow-moving landslide areas, this study collected fundamental data from tree-ring analysis of curved trees in these areas. We collected both upper and lower stem disks to measure the azimuth angles of six trees with growth curvature caused by tension cracks. Additionally, we analyzed various factors in the slow moving landslide area. The geological strata and main constitutive rocks in the study area were anorthosite-formed in the Precambrian period; moreover, there were no intrusive rocks, other geological strata, geological folds, or faults. The talus with weathered rocks was distributed in the upper zone of the slow-moving landslide area. According to annual-ring analysis of curved trees and terrain analysis by satellite imagery, slow-moving landslide occurred from the top to the bottom end of the slope between 1999 and 2011. There was a significant relationship (P < 0.01) between the azimuth angle of cracks caused by the slow-moving landslide and the angle of the curved trees. These results suggest that the occurrence of slow-moving landslides could be confirmed through analysis of annual-rings of curved trees, underground water levels, and terrain (by satellite imagery).

Abandoning Imagination: The Genealogical Aberration in Magical/Realism and Karen Tei Yamashita's Tropic of Orange (상상력의 폐기: 마술적/리얼리즘의 계보와 캐런 테이 야마시타의 『오렌지 북회귀선』)

  • Park, Seonjoo
    • Cross-Cultural Studies
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    • v.42
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    • pp.285-311
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    • 2016
  • This paper examines the constitutive relationship between realism and magical realism using a genealogical approach. Georg $Luk{\acute{a}}cs^{\prime}s$ The Theory of Novel and Gabriel García $M{\acute{a}}rquez^{\prime}s$ One Hundred Years of Solitude, as two founding texts of each genealogy, meet each other obliquely, sharing the most essential features. Even if realism and magical realism appear in opposition to each other in their political, cultural, epistemological outlooks, they in fact constitute the same truth regime in two different guises. Karen Tei Yamashita's Tropic of Orange interrogates this discursive regime of magical/realism, refusing to be contained within it. Her novel de-emphasizes the current idea of solidarity based on identity politics because it cannot resist effectively against the all-reifying power of globalization. Instead, she abandons the idea of imagination itself, and thus, tries to cease the dominant operative of magical/realism. On the temporary vacuum caused from such a conscious act of abandoning imagination, Tropic of Orange posits the urgent need to rethink 'solitude' and 'community', which already have been hopelessly compromised in the history of literary imagination as a global governmentality.

Hsp70 and IKKγ Synergistically Suppress the Activation of NF-κB (Hsp70와 IKKγ에 의한 NF-κB 활성억제의 상승효과)

  • Kim, Mi Jeong;Kim, Ka Hye;Kim, Moon Jeong;Kim, Jin Ik;Choi, Hye Jung;Moon, Ja Young;Joo, Woo Hong;Kim, Dong Wan
    • Journal of Life Science
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    • v.26 no.9
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    • pp.991-998
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    • 2016
  • NF-κB acts as a critical transcription factor for the survival of cells via the induction of antiapoptotic genes. Constitutive activation of NF-κB in many types of solid tumors suggests that the inhibition of NF-κB might prevent or inhibit tumorigenesis. Although a number of studies demonstrated that Hsp70 regulated NF-κB activity, the exact mechanism is not clear. This study investigated the functional relationship of Hsp70 and IKKγ in the regulation of NF-κB activation using expression plasmids of components of the IKK complex. Wild-type and deletion mutants of IKKγ were expressed together with Hsp70, and the combined regulatory effect of Hsp70 and IKKγ on NF-κB activation was assayed. Hsp70 suppressed the activation of NF-κB in a reporter plasmid assay. Hsp70 also suppressed the phosphorylation and degradation of IκBα. The suppressive effect of Hsp70 on NF-κB activation was synergistically elevated by IKKγ. The N-terminal IKKβ binding site, C-terminal leucine zipper, and zinc finger domains of IKKγ were not necessary for the suppressive effect. Furthermore, Hsp70 and IKKγ synergistically suppressed the induction of COX-2 expression by lipopolysaccharides in RAW264.7 cells. These results suggest that overexpression of Hsp70 and IKKγ may be a strategic method for inhibition of NF-κB and related diseases.