• Journal of The Korean Society of Grassland and Forage Science
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• v.21 no.1
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• pp.1-6
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• 2001

This experiment was conducted to evaluated the effect of maturity at harvest on the changes in quality of round baled rye silage at forage experimental field of Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon in 1998. The experimental design was a split-plot design with three replications. The main plots were three different harvest stages : boot, heading and flowering stages, and the subplots were days after ensiling : 1, 2, 3, 5, 10, 30, 45, and 60 days. The wilting period of boot, heading and flowering stages were 1, 0.5 and 0.5 days, respectively. The final pH of rye silage was higher in the order of flowering, boot and heading stages. And pH of flowering stage began to change at early fermentation period, but that of boot and heading stages was delayed 1~2 days. Ammonia-N content of boot stage was highest. and that was increased as fermentation progressed. But Ammonia-N of heading stage was decreased to 30 days. then that was increased after 45 days fermentation. Among fermentation periods, inside temperature of deep place was not affected by external temperature. And that of deep place was increased to 3$0^{\circ}C$ at early fermentation. then decreased as fermentation progressed. However surface temperature was affected by external temperature after 10 days. Acetic acid content was not changed with 5 days by harvest stages, but that of boot stage was increased after 10 days. Butyric acid of boot stage was increased after 5 days. but that of heading stage was increased after 10 days. However lactic acid was increased from 1~2% to 6~8%. Lactic acid bacteria (LAB) of heading and flowering stages were highest at 5 days fermentation, and that of boot stage was highest at 10 days fermentation. The results of this study indicate that fermentation of round baled rye silage occur within 5 days. Therefore, any modification should be applied with an 5 days for high quality of round baled rye silage.

• 한국균학회소식:학술대회논문집
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• 2014.10a
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• pp.9-9
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• 2014

Null mutants generated by targeted gene replacement are frequently used to reveal function of the genes in fungi. However, targeted gene deletions may be difficult to obtain or it may not be applicable, such as in the case of redundant or lethal genes. Constitutive expression system could be an alternative to avoid these difficulties and to provide new platform in fungal functional genomics research. Here we developed a novel platform for functional analysis genes in Magnaporthe oryzae by constitutive expression under a strong promoter. Employing a binary vector (pGOF1), carrying $EF1{\beta}$ promoter, we generated a total of 4,432 transformants by Agrobacterium tumefaciens-mediated transformation. We have analyzed a subset of 54 transformants that have the vector inserted in the promoter region of individual genes, at distances ranging from 44 to 1,479 bp. These transformants showed increased transcript levels of the genes that are found immediately adjacent to the vector, compared to those of wild type. Ten transformants showed higher levels of expression relative to the wild type not only in mycelial stage but also during infection-related development. Two transformants that T-DNA was inserted in the promotor regions of putative lethal genes, MoRPT4 and MoDBP5, showed decreased conidiation and pathogenicity, respectively. We also characterized two transformants that T-DNA was inserted in functionally redundant genes encoding alpha-glucosidase and alpha-mannosidase. These transformants also showed decreased mycelial growth and pathogenicity, implying successful application of this platform in functional analysis of the genes. Our data also demonstrated that comparative phenotypic analysis under over-expression and suppression of gene expression could prove a highly efficient system for functional analysis of the genes. Our over-expressed transformants library would be a valuable resource for functional characterization of the redundant or lethal genes in M. oryzae and this system may be applicable in other fungi.

• Journal of Life Science
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• v.13 no.3
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• pp.314-323
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• 2003

The purpose of this study was to examine the effects of xenobiotic nuclear receptors, CAR, SXR, and PPAR${\gamma}$ on the transcriptional activity of estrogen receptor in human breast cancer cell lines and compare with those in human hepatoma cell line. Two different breast cancer cell lines, MCF-7 and MDA-MB-231 were cultured and effects of CAR, SXR, and PPAR${\gamma}$ on the ER-mediated transcriptional activation of synthetic (4ERE)-tk-luciferase reporter gene were analyzed. Consistent with the previous report, CAR significantly inhibited ER-mediated transactivation and SXR repressed modestly whereas the PPAR${\gamma}$ did not repress the ER-mediated transactivation. However, in breast cancer cells neither of the xenobiotic receptors repressed the ER-mediated transactivation. Instead, they tend to increase the transactivation depending on the cell type and xenobiotic nuclear receptors. In MCF-7, SXR but neither CAR nor PPAR${\gamma}$ slightly increased ER-mediated transactivation whereas in MDA-MB-231, CAR and PPAR${\gamma}$ but not SXR tend to increase the transactivation of the reporter gene. These results indicate that the effects of ER cross-talk by the CAR, SXR, and PPAR${\gamma}$ , are different in breast cancer cells from hepatoma cells. In conclusion, the transcriptional regulation by estrogen can involve different cross-talk interaction between estrogen receptor and xenobiotic nuclear receptors depending on the estrogen target cells.

• The KIPS Transactions:PartB
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• v.10B no.3
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• pp.281-286
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• 2003

A large number of features are collected for problem solving in real life, but to utilize ail the features collected would be difficult. It is not so easy to collect of correct data about all features. In case it takes advantage of all collected data to learn, complicated learning model is created and good performance result can't get. Also exist interrelationships or hierarchical relations among the features. We can reduce feature's number analyzing relation among the features using heuristic knowledge or statistical method. Heuristic technique refers to learning through repetitive trial and errors and experience. Experts can approach to relevant problem domain through opinion collection process by experience. These properties can be utilized to reduce the number of feature used in learning. Experts generate a new feature (highly abstract) using raw data. This paper describes machine learning model that reduce the number of features used in learning using heuristic function and use abstracted feature by neural network's input value. We have applied this model to the win/lose prediction in pro-baseball games. The result shows the model mixing two techniques not only reduces the complexity of the neural network model but also significantly improves the classification accuracy than when neural network and heuristic model are used separately.

• Journal of Korean Philosophical Society
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• v.129
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• pp.23-51
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• 2014

Christine M. Korsgaard believes that constructivism can respond to moral skepticism without depending upon moral realism. The purpose of this paper is to examine Korsgaard's kantian constructivism and her positions on moral realism. According to Korsgaard moral realism cannot answer normative questions in that it sees the function of moral concepts as describing the reality and so accepts the model of applied knowledge for action. In contrast Korsgaard insists that constructivism is better at justifying normativity since it regards moral concepts as representing the solutions to practical problems and so shows that moral principles are necessarily involved in the practical problems of agency. Korsgaard's constructivism has antirealistic elements such as pure proceduralism, the constitutive model to exclude ontological, metaphysical meanings, and the account of human beings as the sources of values. In spite of those antirealistic elements it is difficult to jump to a conclusion that Korsgaard's constructivism is antirealism. Korsgaard, in the early book, The Sources of Normativity, says that kantian constructivism has something to do with a form of realism, or procedural moral realism. And in the following books she argues that constructivism is compatible with realism although she pays attention to the practical implications of constructivism and then sets aside its ontological relevance. That is, Korsgaard does not want that her constructivism results in antirealism. Korsgaard's realism, however, is too weak to be called as realism. There is, also, a question why one would rather take a constructivist approach if one holds on to realism.

• Journal of Plant Biotechnology
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• v.5 no.3
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• pp.143-148
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• 2003

Bax, a mammalian pro-apoptotic member of the Bcl-2 family induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorhodamine123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MSP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to playa novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.

• BMB Reports
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• v.40 no.5
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• pp.715-722
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• 2007

A new full-length cDNA encoding hyoscyamine $6\beta$-hydroxylase (designated as aah6h, GenBank Accession No. EF187826), which catalyzes the last committed step in the scopolamine biosynthetic pathway, was isolated from young roots of Anisodus acutangulus by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of aah6h was 1380 bp and contained a 1035 bp open reading frame (ORF) encoding a deduced protein of 344 amino acid residues. The deduced protein had an isoelectric point (pI) of 5.09 and a calculated molecular mass of about 38.7 kDa. Sequence analyses showed that AaH6H had high homology with other H6Hs isolated from some scopolamine-producing plants such as Hyoscyamus niger, Datura metel and Atropa belladonna etc. Bioinformatics analyses results indicated AaH6H belongs to 2-oxoglutarate-dependent dioxygenase superfamily. Phylogenetic tree analysis showed that AaH6H had closest relationship with H6H from A. tanguticus. Southern hybridization analysis of the genomic DNA revealed that aah6h belonged to a multi-copy gene family. Tissue expression pattern analysis firstly founded that aah6h expressed in all the tested tissues including roots, stems and leaves and indicated that aah6h was a constitutive-expression gene, which was the first reported tissue-independent h6h gene compared to other known h6h genes.

• Journal of Soil and Groundwater Environment
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• v.13 no.1
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• pp.67-76
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• 2008

In this study, a combined model of a water-table fluctuation and a soil moisture content model is proposed for the estimation of groundwater recharge rate at a given location. To evaluate the model, groundwater level data from 4 monitoring wells (Pohang Yeonil, Pohang Kibuk, Suncheon Oeseo, Hongcheon Hongcheon) of National Groundwater Monitoring Network from 1996 to 2005 and precipitation data of corresponding years are used. From the proposed methodology, the groundwater recharge rates are estimated to be from 0.5 to 61.4% for Hongcheon Hongcheon, from 1.1 to 27.4% for Pohang Yeonil, from 5.1 to 41.4% for Pohang Kibuk, and from 1.1 to 8.3% for Suncheon Oeseo. The magnitude of variation of the estimated recharge rate depends on the soil type observed near the stations. The groundwater fluctuation model used in this study includes precipitation as a unique source of water-table perturbation and there may exist corollary limitations. To improve the applicability of the proposed method, a capillary-water content constitutive model for unsaturated fractured rock media may be considered. The proposed recharge rate delineation method is physically based and uses minimum numbers of assumptions. The method may be used as a better substitute for the previous tools for delineating recharge rate of a location using water-table fluctuation method and contribute to national groundwater management plan. Further research on the spatial interpolation of the method is under progress.

• Lingua Humanitatis
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• v.2 no.2
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• pp.223-265
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• 2002

In United States black mothers have consistently been treated as national outsiders, as women whose children, although ostensibly entitled to full citizenship, are in practice rarely provided with equal protection within the nation′s borders or under its laws. From the time he began writing in the aftermath of the failures of national Reconstruction, the African American public intellectual and political activist W. E. B. Du Bois realized that a truly effective anti-racist politics would also have to contend with the particular ways in which U.S. racism targeted black mothers. In short, he understood that an effective anti-racism would necessarily have to be a form of anti-sexism. This article examines the myriad ways in which Du Bois attempted to reconstruct the relationship between race and reproduction in the interest of producing anti-racist, anti-nationalist, as well as internationalist thinking. In so doing it treats the various representations of black maternity and child birth that Du Bois created, and elaborates on the rhetorical and political function of these representations in combating the racialization of national belonging on the one hand, and in articulating universal black citizenship, or what this article theorizes as racial globality on the other. The article begins by considering Du Bois′s attempts to transcend ideas about the racialized reproductive body as a source of national belonging within the United States, particularly his efforts to contest the idea of the reconstructing nation as a white nation reproduced exclusively by white women. Through analysis of Du Bois′s depiction of the birth and death of his son in his monumental work The Souls of Black Folk (1903) it demonstrates his reluctance to build an anti-racist politics founded on the idea that belonging within the nation is something that can be bestowed by one′s mother. The article proceeds by turning to Du Bois less well-known romantic novel, Dark Princess (1928) in which, by contrast, he depicts the birth of a "golden chi1d" who belongs not only within the United States, but within the world. This child, the son of an African American man and an Indian Princess, is cast as a messenger and messiah of a utopian alliance between pan-Asia and pan-Africa. In exploring the relationship between these two reproductive portraits, the article moves from a discussion of Du Bois′s critique of the ideological construction of the U.S. as a white nation reproduced by white progenitors, to an examination the literary figuration of a b1aek mother out of whose womb a black diasporic anti-imperialist alliance springs. In contrast to previous scholarship, which has tended to focus on the critique of U.S. racial nationalism that Du Bois expressed in his early work, or on the internationalism that he later embraced, this article pays close attention to how Du Bois′s anti-nationalist and internationalist politics together subtended by subtle, but constitutive, sexual politics.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.28 no.1
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• pp.1-8
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• 2015

In this paper, computational evaluation method for fatigue crack growth rate(FCGR) based on material viscoplastic-damage model is proposed. Viscoplastic-damage model expressing material constitutive behavior of 7% nickel steel is introduced and is implemented into commercial finite element analysis(FEA) code, ABAQUS, as a user defined material subroutine(UMAT) for application in the FEA environments. Verification of developed UMAT and material parameters of material model are carried out by uniaxial tensile test simulations of 7% nickel steel. Moreover, jump-in-cycles procedure and rearrangement of critical damage are employed and also implemented to the ABAQUS UMAT for fatigue damage analysis. Typical FCGR test results such as relationship between crack length and number of cycles and relationship between da/dN and ${\Delta}K$ could be obtained from FCGR test simulation using developed UMAT and these results are compared with experimental results in order to verify of proposed computational method.