• Journal of the Korean Society of Environmental Restoration Technology
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• v.20 no.5
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• pp.43-58
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• 2017

This study aims to provide basic understanding for evaluating the quantitative and qualitative progress of national protected areas, through the theoretical review of Aichi Biodiversity Target 11 in order to comply with recommendations of international community and to conserve biodiversity. As a result of the study, Aichi Biodiversity Target 11 sets out the specific contents that the Parties should achieve for protected areas by identifying them as temporal and spatial goals. The temporal goal, the time schedule for achieving the goal, is 2020, and the spatial goal is divided into quantitative expansion goals and qualitative improvement goals. The quantitative expansion goals present the target coverage of protected areas separately terrestrial and marine. The qualitative improvement goals include the target areas for conservation and five conservation considerations. The conservation targets focus on the important areas with regard to biodiversity and ecosystem services. The five conservation considerations mean effective management, equitable management, ecological representativeness, connectivity, and integration into the landscape and seascape for protected areas. Finally, it suggests that two tracks of protected areas and other effective area-based conservation measures(OECMs) should be used as conservation measures to build an integrated system. The results of this study can be applied to quantitative and qualitative evaluation methods for protected areas and it can contribute to achieve quantitative expansion goals and qualitative improvement goals for them.

• Journal of Environmental Policy
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• v.6 no.2
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• pp.27-45
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• 2007

This paper presents a method for developing the performance assessment of energy conservation and efficiency based on baseline and target adjustment approach. Energy consumption is related with output level and production structure. This paper suggests improvement for target establishment and performance assessment with regard to real output level in target year. Numerical illustrations of baseline and target adjustment method are shown different compared with the general assessment results. This study will contribute to applying the conformity assessment and Greenhouse Gas Emissions verification system.

• Asian-Australasian Journal of Animal Sciences
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• v.33 no.2
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• pp.219-229
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• 2020

Objective: Considering the physiological and clinical importance of leptin receptor (LEPR) in regulating obesity and the fact that porcine LEPR expression is not known to be controlled by lncRNAs and miRNAs, we aim to characterize this gene as a potential target of SSC-miR-323 and the lncRNA TCONS_00010987. Methods: Bioinformatics analyses revealed that lncRNA TCONS_00010987 and LEPR have SSC-miR-323-binding sites and that LEPR might be a target of lncRNA TCONS_00010987 based on cis prediction. Wild-type and mutant TCONS_00010987-target sequence fragments and wild-type and mutant LEPR 3'-UTR fragments were generated and cloned into pmiRRB-REPORT^TM-Control vectors to construct respective recombinant plasmids. HEK293T cells were co-transfected with the SSC-miR-323 mimics or a negative control with constructs harboring the corresponding binding sites and relative luciferase activities were determined. Tissue expression patterns of lncRNA TCONS_00010987, SSC-miR-323, and LEPR in Anqing six-end-white (AQ, the obese breed) and Large White (LW, the lean breed) pigs were detected by real-time quantitative polymerase chain reaction; backfat expression of LEPR protein was detected by western blotting. Results: Target gene fragments were successfully cloned, and the four recombinant vectors were constructed. Compared to the negative control, SSC-miR-323 mimics significantly inhibited luciferase activity from the wild-type TCONS_00010987-target sequence and wild-type LEPR-3'-UTR (p<0.01 for both) but not from the mutant TCONS_00010987-target sequence and mutant LEPR-3'-UTR (p>0.05 for both). Backfat expression levels of TCONS_00010987 and LEPR in AQ pigs were significantly higher than those in LW pigs (p<0.01), whereas levels of SSC-miR-323 in AQ pigs were significantly lower than those in LW pigs (p<0.05). LEPR protein levels in the backfat tissues of AQ pigs were markedly higher than those in LW pigs (p<0.01). Conclusion: LEPR is a potential target of SSC-miR-323, and TCONS_00010987 might act as a sponge for SSC-miR-323 to regulate LEPR expression.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.7
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• pp.922-929
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• 2019

Objective: Mutations in low-density lipoprotein receptor (LDLR), which encodes a critical protein for cholesterol homeostasis and lipid metabolism in mammals, are involved in cardiometabolic diseases, such as familial hypercholesterolemia in pigs. Whereas microRNAs (miRNAs) can control LDLR regulation, their involvement in circulating cholesterol and lipid levels with respect to cardiometabolic diseases in pigs is unclear. We aimed to identify and analyze LDLR as a potential target gene of SSC-miR-20a. Methods: Bioinformatic analysis predicted that porcine LDLR is a target of SSC-miR-20a. Wild-type and mutant LDLR 3'-untranslated region (UTR) fragments were generated by polymerase chain reaction (PCR) and cloned into the pGL3-Control vector to construct pGL3 Control LDLR wild-3'-UTR and pGL3 Control LDLR mutant-3'-UTR recombinant plasmids, respectively. An miR-20a expression plasmid was constructed by inserting the porcine premiR-20a-coding sequence between the HindIII and BamHI sites in pMR-mCherry, and constructs were confirmed by sequencing. HEK293T cells were co-transfected with the miR-20a expression or pMR-mCherry control plasmids and constructs harboring the corresponding 3'-UTR, and relative luciferase activity was determined. The relative expression levels of miR-20a and LDLR mRNA and their correlation in terms of expression levels in porcine liver tissue were analyzed using reverse-transcription quantitative PCR. Results: Gel electrophoresis and sequencing showed that target gene fragments were successfully cloned, and the three recombinant vectors were successfully constructed. Compared to pMR-mCherry, the miR-20a expression vector significantly inhibited wild-type LDLR3'-UTR-driven (p<0.01), but not mutant LDLR-3'-UTR-driven (p>0.05), luciferase reporter activity. Further, miR-20a and LDLR were expressed at relatively high levels in porcine liver tissues. Pearson correlation analysis revealed that porcine liver miR-20a and LDLR levels were significantly negatively correlated (r = -0.656, p<0.05). Conclusion: LDLR is a potential target of miR-20a, which might directly bind the LDLR 3'-UTR to post-transcriptionally inhibit expression. These results have implications in understanding the pathogenesis and progression of porcine cardiovascular diseases.

• Journal of architectural history
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• v.20 no.6
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• pp.147-168
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• 2011

The objective of this study is to establish the method of conservation for traditional Korean settlements, based on the field surveys on the representative traditional settlements in Korea and China. The method is approached from grasping the conservation mechanism in which non-physical aspects deeply influence physical ones. The study has analyzed the characteristics and problems in the current methods of conservation for the traditional settlements, through comparing conservation practices at the World Heritage-class settlements such as Yangdong, Hahoi, and Oeam villages in Korea and Xidi-cun, Hong-cun, and Dangjia-cun in China. As a consequence of the study, the conservation method for the traditional Korean settlements is established as follows; Firstly, the conservation targets are classified into 4 types in terms of the relations of elements; individual, correspondent, sequential, and network elements. Secondly, the conservation strategies include the structuralizations of targets, by means of sequencing and networking. Thirdly, the conservation programs are made in consideration of the sustenance, transformation, replacement, and extinction of traditional use patterns.

• Genomics & Informatics
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• v.3 no.3
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• pp.53-62
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• 2005

MicroRNAs play an important role in regulating gene expression, but their target identification is a difficult task due to their short length and imperfect complementarity. Burge and coworkers developed a program called TargetScan that allowed imperfect complementarity and established a procedure favoring targets with multiple binding sites conserved in multiple organisms. We improved their algorithm in two major aspects - (i) using well-defined UTR (untranslated region) database, (ii) examining the extent of conservation inside the 3' UTR specifically. Average length in our UTR database, based on the ECgene annotation, is more than twice longer than the Ensembl. Then, TargetScan was used to identify putative binding sites. The extent of conservation varies significantly inside the 3' UTR. We used the 'tight' tracks in the UCSC genome browser to select the conserved binding sites in multiple species. By combining the longer 3' UTR data, TargetScan, and tightly conserved blocks of genomic DNA, we identified 107 putative target genes with multiple binding sites conserved in multiple species, of which 85 putative targets are novel.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.57-65
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• 2018

The study is conducted to establish the National Protected Areas Integrated System(NPAIS) which includes National Protected Areas(NPAs) and other conservation measures in terms of effective ways for biodiversity conservation. Additionally, it is carried out to evaluate progress toward quantitative expansion goals in Aichi biodiversity Target 11. The NPAIS consists of NPAs and other effective area-based conservation measures(OECMs). There are two different types of OECMs. OECMs type I, including water-source protection Areas(WPA), riparian zones(RZ), fishery-resource protection zones (FPZ), and urban natural park zones(UNPZ), is a potential protected area which is recommended to be incorporated into the NPAs for effective management. OECMs type II means development restriction zones(DRZ), natural recreation forests(NRF), and buffer zones for Korea national arboretum(BKNA). As a result of evaluating the quantitative expansion goals of the NPAIS, terrestrial and inland water protected areas exceed 17% of the quantitative goal in Aichi biodiversity Target 11. The larger the area of individual OECMs and the lower the degree of overlap with NPAs, the higher the contribution of them to the terrestrial and inland water protected areas. DRZ contributes to enlarge more than 3% of quantitative expansion. And RZ and NRF contribute more than 1%. The marine protected areas are expanded by $1,425km^2$ through FPZ, but the expanded area is very small as comparing with the total marine area. It adds only 0.321% to the quantitative expansion. The rest of OECMs also has very poor quantitative expansion contributions in the marine area. Consequently, the NPAIS is effective for quantitative expansion of land areas, but not for marine areas.

• 보존과학연구
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• s.26
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• pp.203-211
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• 2005

The National Research Institute of Cultural Heritage(NRICH) conducted the Asia Cooperation Programme in Conservation Science(ACPCS) to facilitate and promote the regional cooperation in the conservation of cultural heritage among Asian countries. This course was promoted to provide an opportunity to study and work together, also to share a knowledge and experience in conservation field. The target countries were from 17 Asian countries, and this year our selection committee selected 2 specialist in cultural heritage conservation field and selected persons had participated in the ACPCS course during three months. NRICH provided the round-trip airline tickets, overseas travel accident insurance and a living allowance to the course participants. And we requested programme announcement to Korean National Commission for UNESCO and Ministry of Foreign Affairs and Trade to progress favorably. This course period was three months from 29 August to 25 November, and the fields were conservation and restoration of tangible cultural heritage such as metal conservation, stone conservation, material analysis, DNA analysis, dating, biological control management of cultural heritage, environmental monitoring and so on.

• Journal of the Korean Institute of Landscape Architecture
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• v.25 no.4
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• pp.158-170
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• 1998

In this study, we divide greenspace in Suwon city into three parts, that is, the conservation part, the maintenance part and the creation part by considering the quantitative aspect of greenspace and the present targets. To do it, two steps are taken. Firstly, assuming that the target year is 2007, the final target of greenspace area is established by combining targets presented in present state survey, the evaluation of resident perception and the future population estimates. The target of greenspace area in present state survey is 7,887,062$m^2$+$\alpha$ and that of greenspace area demanded in the evaluation of resident perception is 8,242,419$m^2$. Demand of greenspace area based on population estimates is 4,227,513$m^2$. When the greenspace needed in the city is added, the target may be more than 8,242,419$m^2$ which is demanded in the evaluation of resident perception. Outcome of the present state survey, 7,887,062$m^2$ + $\alpha$, is considered to be enough to accomodate the greenspace area demanded in the evaluation of resident perception and the population estimates. Therefore, targets of greenspace in Suwon City was established based on the area of greenspace analyzed in the present state survey. Secondly, the target of greenspace is divided into three parts, that is, the conservation part, the maintenance part and creation part. And the methods to attain the target are presented. In order to set desirable targets of urban greenspace, it is necessary to combine the outcome of present state survey, the resident perception, the population estimates and the various urban conditions. If the results of this study are applied to the urban planning or other related planning, the urban ecosystem will be protected and the biotope will be created.

• Journal of Korean Society on Water Environment
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• v.29 no.1
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• pp.36-44
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• 2013

HSPF model based on BASINS was applied to analyze effects of watershed management measures for water quality conservation in the Hwaseong Reservoir watershed. The model was calibrated against the field measurements of meteorological data, streamflow and water qualities ($BOD_5$, T-N, T-P) at each observatory for 4 years (2007-2010). The water quality characteristics of inflow streams were evaluated. The 4 scenarios for the water quality improvement were applied to inflow streams and critical area from water pollution based on previous researches. The reduction efficiency of point and non-point sources in inflow streams was evaluated with each scenario. The results demonstrate that the expansion of advanced treatment system within wastewater treatment plants (WWTPs) and construction of pond-wetlands would be great effective management measures. In order to satisfactory the target water quality of reservoir, the measures which can control both point source and non-point source pollutants should be implemented in the watershed.