• 한국환경생태학회지
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• 제17권2호
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• pp.133-143
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• 2003

본 연구 결과 울릉도에 자생하는 섬개야광나무의 자생지를 현재 천연기념물로 지정되어 있는 곳 외에 추가로 탐문조사와 현지조사를 통하여 새로운 자생지 두 군데를 찾아내었다. 현지 조사결과 남양 일몰대는 자생지가 일몰대 부근에 위치하여 사람에 의한 체손이 우려되며, 통구미의 자생지는 자생지의 위치가 산 능선부에 위치하기 때문에 사람의 접근이 불가능하고 치수의 생육이 좋은 것으로 밝혀졌다. 각 자생지에 생육하고 있는 개체는 약 25개체에서 50개체이며, 앞으로 기존의 천연기념물지역을 복원하기 위하여 유용한 정보를 제공할 것으로 판단된다. 섬개야광나무의 형태적 분석결과 꽃의 경우 지역별로 화판장과 화판폭의 차이가 없었지만, 잎의 경우에는 엽장. 엽폭. 엽면적 모두 3개지역에서 차이를 나타내었다 이는 섬개야광나무가 다른 식물과 달리 서식처가 분획화되어 생육하는 지역의 환경적 특징에 의해서 잎의 모습이 차이가 나는 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권7호
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• pp.946-951
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• 2003

The Genetic diversity and relationship of native Siri (Bos indicus) cattle populations of Bhutan were evaluated using 20 microsatellite markers. A total of 120 Siri cattle were sampled and were grouped into four populations according to their geographical locations which were named Siri West, Siri South, Siri Central and Siri East cattle. For each, 30 individuals were sampled. In addition, 30 samples each of Indian Jaba (B. indicus), Tibetan Goleng (B. taurus), Nepal Hill cattle (B. indicus), Holstein Friesian (B.taurus) and Mithun (B. frontalis) were typed. The mean number of alleles per loci (MNA) and observed heterozygosity (Ho) were high in the Siri populations ($MNA=7.2{\pm}0.3$ to $8.9{\pm}0.5$ and $Ho=0.67{\pm}0.04$ to $0.73{\pm}0.03$). The smallest coefficient of genetic differentiation and genetic distance ($F_{ST}=0.015$ and $D_A=0.073$) were obtained between Siri West and Siri Central populations. Siri East population is genetically distinct from the other Siri populations being close to the Indian Jaba ($F_{ST}=0.024$ and $D_A=0.084$). A high bootstrap value of 96% supported the close relationship of Siri South, Siri Central and Siri West, while the relationship between Siri East and Jaba was also supported by a high bootstrap value (82%). Data from principal component analysis and individual assignment test were in concordance with the inference from genetic distance and differentiation. In conclusion we identified two separate Siri cattle populations in Bhutan at the genetic level. One population included Siri cattle sampled from the West, Central and South of the country and the other Siri cattle was sampled from the East of the country. We suggest that Siri cattle conservation program in Bhutan should focus on the former population as it has received less genetic influence from other cattle breeds.

• 식물분류학회지
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• 제47권1호
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• pp.11-22
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• 2017

Prangos fedtschenkoi (Regel et Schmalh.) Korovin (Apiaceae) is an endemic species for mountainous Middle Asia, which is both a rare and useful plant. Organic extractions from this species are being used in pharmaceutics and cosmetology. In recent years, P. fedtschenkoi distribution area has considerably decreased, presumably, resulting from human activities such as agriculture, construction works, overgrazing and collection from wild for pharmaceutic purposes. Six populations were found in Uzbekistan and their genetic divergence and differentiation were studied with 10 inter-simple sequence repeat (ISSR) markers, selected out of 101. Totally 166 amplified ISSR fragments (loci) were revealed, of which 164 were polymorphic. Relatively moderate level of polymorphism was found at population level with polymorphic bands ranging from 27.71% to 47.59%. Mean P = 39.05%, $N_a=1.40$, $N_e=1.25$, S.I. = 0.21, and $H_e=0.14$ were revealed for all loci across six populations. AMOVA showed higher variation among populations (62%) than within them (38%). The Bayesian model determined 5 clusters, or genetic groups. The posteriori distribution of the Theta II estimator detected full model identifying high inbreeding, intensified by low gene flow (Nm = 0.3954). Mantel test confined population 6 as distinct cluster corresponding to geographic remoteness (R = 0.5137, $p{\leq}0.005$). Results were used as the bases for developing conserve measures to restore populations.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.49-57
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• 2019

Objective: The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII], Barred Rock [BARI, BARII], Columbian Rock [COL], and line 54 [L-54]) with microsatellite markers. Furthermore, the study aims to employ its findings to discuss the possibilities for the conservation and sustainable use of these lines that have been bred as closed populations for a long time. Methods: In the present study, a total number of 180 samples belonging to RIRI (n = 30), RIRII (n = 30), BARI (n = 30), BARII (n = 30), L-54 (n = 30), and COL (n = 30) lines were genotyped using 22 microsatellite loci. Microsatellite markers are extremely useful tools in the identification of genetic diversity since they are distributed throughout the eukaryotic genome in multitudes, demonstrate co-dominant inheritance and they feature a high rate of polymorphism and repeatability. Results: In this study, we found all loci to be polymorphic and identified the average number of alleles per locus to be in the range between 4.41 (BARI) and 5.45 (RIRI); the observed heterozygosity to be in the range between 0.31 (RIRII) and 0.50 (BARII); and $F_{IS}$ (inbreeding coefficient) values in the range between 0.16 (L-54) and 0.46 (RIRII). The $F_{IS}$ values obtained in this context points out to a deviation from Hardy-Weinberg equilibrium due to heterozygote deficiency in six different populations. The Neighbour-Joining tree, Factorial Correspondence Analysis and STRUCTURE clustering analyzes showed that six brown layer lines were separated according to their genetic origins. Conclusion: The results obtained from the study indicate a medium level of genetic diversity, high level inbreeding in chicken lines and high level genetic differentiation between chicken lines.

• 한국산림과학회지
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• 제102권3호
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• pp.428-436
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• 2013

우리나라 희귀식물인 꼬리겨우살이(꼬리겨우살이과) 자생 집단의 효율적인 보전대책을 수립하기 위하여 자생 집단의 분포와 자생지 특성을 구명하고, 이를 토대로 보전지위 평가를 수행하였다. 한반도에서 꼬리겨우살이는 지리산에서부터 백두대간을 따라 설악산까지 분포하였으며, 전체 개체 중 97.8%가 강원도 지역에 집중분포 하였다. 그리고 꼬리겨우살이의 1,385개체가 480개의 기주식물에 기생하는 것으로 확인되었으며, 해발 353~1,250 m의 범위에서 광이 충분한 능선부위에 분포하는 것으로 관찰되었다. 꼬리겨우살이의 기주식물 범위는 5과 6속 10분류군(9종 1변종)이었으며, 그중 신갈나무에서 81.5%가 확인되어 가장 선호하는 결과를 보였다. 꼬리겨우살이의 분포와 자생지 특성조사 결과를 토대로 IUCN 적색목록 기준에 의한 보전지위를 평가한 결과 지역수준에서 VU등급으로 분류되었다. 그리고 꼬리겨우살이의 최대 분포지역인 설악산과 태기산, 오대산 지역에 대해 현지 내 보전구역으로 설정하여 집중보호를 해야 할 것으로 판단되었다. 이와 같이 꼬리겨우살이의 자생 집단의 지속가능한 보존을 위한 보전전략과 이를 위한 전략수립을 도출하였다.

• 한국환경생태학회지
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• 제35권2호
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• pp.193-203
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• 2021

인간의 개발로 점차 멸종위기 생물종이 증가하고 있어, 충분한 보호지역의 선제적 확보가 중요한 상황이다. 따라서 본 연구에서는 인간의 개발영향을 고려했을 때 앞으로 보호지역 선정 시 고려해야 할 보전 우선순위지역을 확인하였다. 보전 우선순위 도출은 보전계획 의사결정 지원 소프트웨어인 MARXAN을 이용하여 기존 보호지역 포함 여부와 개발영향 반영여부를 기준으로 총 네 가지의 시나리오로 분석하였다. 개발영향은 개발면적 비율, 인구밀도, 도로망 체계, 교통량을 이용하여 도출하였으며, 생물종 보전 대상 지역은 제 3차 전국자연환경조사 자료의 조류, 포유류, 양서파충류의 출현자료를 이용하여 도출한 서식적합지역을 이용하였다. 이 두 가지 요인을 입력 자료로 기계학습 기반 최적화방법론을 이용하여 보전 우선순위 지역을 도출하였다. 연구결과, 멸종위기 생물종을 보전하는데 중요한 역할을 할 것으로 기대되는 지역이 기존 보호지역과 떨어진 지역에서 다수 나타났으며, 개발영향을 고려했을 때는 보전우선순위 지역이 파편화되어 나타남을 알 수 있었다. 개발영향과 기존보호지역을 모두 고려했을 때에도 기존 보호지역 주변으로 이미 도로개발이 많이 이뤄져 기존 보호지역과는 떨어진 지역에서 우선순위가 높게 나타났다. 따라서 개발영향을 고려하여 멸종위기종 보호하기 위해서는 기존 보호지역 주변 이외의 지역도 검토해볼 필요가 있으며, 파편화되어 나타나는 보전 우선순위지역에 대한 대응방안 모색이 필요함을 알 수 있었다.

• Animal Bioscience
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• 제36권5호
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• pp.692-703
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• 2023

Objective: The main goals of this investigation were to i) assess the population structure and genetic diversity and ii) determine the efficiency of the ongoing breeding program in a closed flock of Angora rabbits through pedigree analysis. Methods: The pedigree records of 6,145 animals, born between 1996 to 2020 at NTRS, ICAR-CSWRI, Garsa were analyzed using ENDOG version 4.8 software package. The genealogical information, genetic conservation index and parameters based on gene origin probabilities were estimated. Results: Analysis revealed that, 99.09% of the kits had both parents recorded in the whole dataset. The completeness levels for the whole pedigree were 99.12%, 97.12%, 90.66%, 82.49%, and 74.11% for the 1st, 2nd, 3rd, 4th, and 5th generations, respectively, reflecting well-maintained pedigree records. The maximum inbreeding, average inbreeding and relatedness were 36.96%, 8.07%, and 15.82%, respectively. The mean maximum, mean equivalent and mean completed generations were 10.28, 7.91, and 5.51 with 0.85%, 1.19%, and 1.85% increase in inbreeding, respectively. The effective population size estimated from maximum, equivalent and complete generations were 58.50, 27.05, and 42.08, respectively. Only 1.51% of total mating was highly inbred. The effective population size computed via the individual increase in inbreeding was 42.83. The effective numbers of founders (f_e), ancestors (f_a), founder genomes (f_g) and non-founder genomes (f_ng) were 18, 16, 6.22, and 9.50, respectively. The fe/fa ratio was 1.12, indicating occasional bottlenecks had occurred in the population. The six most influential ancestors explained 50% of genes contributed to the gene pool. The average generation interval was 1.51 years and was longer for the sire-offspring pathway. The population lost 8% genetic diversity over time, however, considerable genetic variability still existed in the closed Angora population. Conclusion: This study provides important and practical insights to manage and maintain the genetic variability within the individual flock and the entire population.

• Journal of Forest and Environmental Science
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• 제28권3호
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• pp.158-169
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• 2012

Sabah has experienced a rapid decline in the extent of forest cover. The precise impact of habitat loss on the conservation status of the plants of Sabah is uncertain. In this study we use the niche modelling algorithm MAXENT to construct preliminary, revised and final ecological niche models for Dipterocarpus lamellatus and Dipterocarpus ochraceus and combined these models with data on current land-use to derive conservation assessments for each species. Preliminary models were based on herbarium data alone. Ground surveys were conducted to evaluate the performance of these preliminary models, and a revised niche model was generated from the combined herbarium and ground survey data. The final model was obtained by constraining the predictions of the revised models by filters. The range overlap between the preliminary and revised models was 0.47 for D. lamellatus and 0.39 for D. ochraceus, suggesting poor agreement between them. There was substantial variation in estimates of habitat loss for D. ochraceus, among the preliminary, revised and constrained models, and this has the potential to lead to incorrect threat assessments. From these estimates of habitat loss, the historic distribution and estimates of population size we determine that both species should be classified as Critically Endangered under IUCN Red List guidelines. Our results suggest that ground-truthing of ecological niche models is essential, especially if the models are being used for conservation decision making.

• 환경영향평가
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• 제17권2호
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• pp.133-141
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• 2008

The loss, alteration, and fragmentation of habitat have led to a reduction of biodiversity. The growing awareness of the negative effects of habitat fragmentation on natural systems has resulted in conservation strategy that is concerned with not only population and habitat level but also ecosystem and landscape level. Especially, ecological network to link core areas or major habitat patches is one of the most important issues. Recently, landscape connectivity is increasingly used in decision making for fragmented landscape management in order to conserve the biodiversity in the regional scale. The objective of this study was to find potential forest as a ecological corridor in Go-yang city, Gyung-gi province using cost-distance modelling method that can measure connectivity based on animal movement. 'Least cost-distance' modelling based on functional connectivity can be useful to establish ecological network and biodiversity conservation plan. This method calculates the distance modified with the cost to move between habitat patches based on detailed geographical information on the landscape as well as behavioural aspects of the animal movement. The least cost-distance models are based on two biologically assumptions: (1) dispersers have complete knowledge of their surroundings, and (2) they do select the least cost route from this information. As a result of this study, we can find wildlife moving route for biodiversity conservation. The result is very useful for long-term aspect of biodiversity conservation plan in regional scale, because this is reflection of geographical information and behavioural aspects of the animal movement.

• Asian-Australasian Journal of Animal Sciences
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• 제31권10호
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• pp.1558-1564
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• 2018

Objective: We report monitoring conservation effect for a Chinese indigenous chicken (Langshan) breed using major histocompatibility complex (MHC) and DNA barcords. Methods: The full length of MHC B-G gene and mitochondrial cytochrome oxidase I (COI) gene in generations 0, 5, 10, 15, 16, and 17 was measured using re-sequencing and sequencing procedures, respectively. Results: There were 292 single nucleotide polymorphisms of MHC B-G gene identified in six generations. Heterozygosity (He) and polymorphic information content (PIC) of MHC B-G gene in generations 10, 15, 16, and 17 remained stable. He and PIC of MHC B-G gene were different in six generations, with G10, G15, G16, G17 >G5>G0 (p<0.05). For the COI gene, there were five haplotypes in generations 0, 5, 10, 15, 16, and 17. Where Hap2 and Hap4 were the shared haplotypes, 164 individuals shared Hap2 haplotypes, while Hap1 and Hap3 were the shared haplotypes in generations 0 and 5 and Hap5 was a shared haplotype in generations 10, 15, 16, and 17. The sequence of COI gene in 6 generations was tested by Tajima's and D value, and the results were not significant, which were consistent with neutral mutation. There were no differences in generations 10, 15, 16, and 17for measured phenotypic traits. In other generations, for annual egg production, with G5, G10, G15, G16, G17>G0 (p<0.05). For age at the first egg and age at sexual maturity, with G10, G15, G16, G17>G5>G0 (p<0.05). Conclusion: Combined with the results of COI gene DNA barcodes, MHC B-G gene, and phenotypic traits we can see that genetic diversity remained stable from generations 10 to 17 and the equimultiple random matching pedigrees conservation population conservation effect of Langshan chicken was effective as measured by these criteria.