• Title/Summary/Keyword: connective tissue

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Effect of Acid Treatment Process on the Physicochemical Properties of Gelatin Extracted from Pork Skin (산처리 공정에 따라 추출한 돈피 젤라틴의 이화학적 특성에 관한 연구)

  • Yeom Geun-Woong;J Andrieu;Min Sang-Gi
    • Food Science of Animal Resources
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    • v.24 no.3
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    • pp.266-272
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    • 2004
  • The objective of this study was to investigate the physicochemical characteristics of gelatin extracted from pork skin under soaking in various acid solutions (lactic acid, acetic acid, and citric acid). Gelatin sol was extracted at 8$0^{\circ}C$, frozen at -2$0^{\circ}C$ and lyophilized it for 3 days to be completely dried in freeze drying unit. In the evaluation of gelatin quality, gelatin soaked in citric acid showed higher L- and a-values than those of any other gelatin (p<0.05). Gelatin treated by acetic acid showed the highest gel strength, cohesiveness, and brittleness. The content of hydroxyproline amino acid in gelatin treated by acetic acid was larger than one of gelatin treated in lactic and citric acid in order. From the experimental results, the highest quality of gelatin in all of period, which was soaked in acetic acid and lactic acid, has a more good quality than gelatin soaked in citric acid.

Evaluation of Meat Color and Physiochemical Characteristics in Forequarter Muscles of Holstein Steers

  • Moon, Sung Sil;Seong, Pil-Nam;Jeong, Jin Young
    • Food Science of Animal Resources
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    • v.35 no.5
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    • pp.646-652
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    • 2015
  • The beef forequarter muscle comprises approximately 52% of carcass weight. The objective of this study was to evaluate the physiochemical characteristics and meat color from forequarter muscle of Holstein steers. Fifteen forequarter muscles were trimmed of external connective tissue and fat. An experimental group of eight Holstein steers was assessed using meat color, water-holding capacity, drip loss, and Warner-Bratzler shear force value at the same quality grade. The M. omotransversarius (0.45 kg) had the highest (p<0.05) lightness (L*) value, whereas the M. teres major (0.4 kg) and M. triceps brachii (caput laterale) (0.52 kg) had the lowest (p<0.05) values. The M. semispanitus capitus (1.48 kg), which is a neck muscle, had the highest values for both redness (a*) and yellowness (b*), whereas the lowest (p<0.05) values were for the M. teres major. The M. omotransversarius, M. latissimus dorsi (1.68 kg), and M. rhomboideus (1.2 kg) were ranked high (p<0.05) in water-holding capacity. The drip loss value was the highest for the M. longissimus dorsi thoracis (p<0.05; 1.86 kg), while the M. infraspinatus (2.28 kg), M. supraspinatus (1.38 kg), M. brachiocephalicus (1.01 kg), and M. pectoralis superficialis (1.18 kg) had the lowest (p<0.05). The Warner-Bratzler shear force value indicated that the M. pectoralis profundus (3.39 kg), M. omotransversarius, and M. brachiocephalicus were the toughest (p<0.05), whereas the M. subscapularis (0.86 kg), M. longissimus dorsi thoracis, M. teres major, and M. infraspinatus were the most tender cuts (p<0.05). Here, muscle type explained most of the variability in the forequarter physiochemical characteristics. Thus, our findings suggest that these muscle profile data will allow for more informed decisions when selecting individual muscles to produce value-added products from Holstein steers.

Effect of Betamethasone on the Pulmonary Pressure-Volume Curve in Unilateral Pneumonectomized Rabbits (Betamethasone이 일측폐장절제 가토폐장의 압력-용적곡선에 미치는 영향)

  • Lee, Young-Man;Lee, Suck-Kang
    • The Korean Journal of Physiology
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    • v.17 no.1
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    • pp.23-28
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    • 1983
  • In order to clarify the effect of steroid on the pulmonary pressure-volume curve in the pneumonectomized rabbit, the right side pneumonectomy was performed under general anesthesia with pentobarbital sodium and the remaining lung was excised on the fifth day after surgery. The intrapulmonic pressure in the excised left lung was measured at 20, 25, and 30 ml lung volume during inflation and deflation period. In the steroid treated group, the intrapulmonic pressure at 20, 25, and 30 ml lung volume during inflation was significantly decreased compared with intrapulmonic pressure of the control group. But in the pneumonectomy group the decreasing tendency of the pressure was observed. During deflation, the intrapulmonic pressure at 25 ml and 20 ml was not significantly different from the control group in pneumonectomy and steroid treated pneumonectomy group. And the lung weight was measured in each experimental group and also the lung weightbody weight ratio(L/B) was calculated. The lung weight was significantly increased in both pneumonectomy and steroid treated pneumonectomy group. Calculated L/B was increased significantly in both group. Above results suggest that steroid increases the secretion of pulmonary surfactant or affects the catabolic effect on protein metabolism of connective tissue in lung parenchyma.

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Electron Microscopical Description on the Egg Stalk-like Structure of the Rockfish, Sebastes inermis (Teleostei: Scorpaenidae) (볼락 (Sebastes inermis) 난병 유사구조의 전자현미경적 기재)

  • Lee Jung Sick;Chang Young Jin
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.35 no.2
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    • pp.130-134
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    • 2002
  • The ovary of the rockfish, Sebastes inermis was cystovarian condition and consists of numerous ovarian lobules. Previtellogenic oocytes were embedded in the connective tissue of the ovarian lobules. As the oocytes grows, it protruded into the ovarian cavity and form grapes cluster-like structure with egg stalk-like structure. Yolk granules and zona radiata were not observed in the embedded early oocytes about 20 $\mu$m in diameter. In the oocytes about 80 $\mu$m in diameter, yolk granules with electron dense were abundant in the ooplasm near the egg stalk-like structure. However, pinocytosis was not obsened in the pore canal system of the zona radiata. In this stage, microfilaments, developed endoplasmic reticula, tubular mitochondria, granular materials with low electron density and active multivesicular bodies were observed in the egg stalk-like structure. This results suggest that the egg stalk-like structure of the rockfish are related with the oocyte attachment and exogenous vitellogenesis of the intial vitellogenic oocyte.

A Study on Attitude Toward Oriental Medical Care Treatment Provided by Health Centers in a Designated Rural Areas (군보건소 한방진료실 이용자의 한방진료에 대한 태도분석)

  • Kim, Jin-Soon;Kim, Eun-Joo
    • Journal of agricultural medicine and community health
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    • v.17 no.1
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    • pp.25-33
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    • 1992
  • Most of oriental medical care resources such as doctors and facilities are distributed in urban areas and approximately ten percent of them is in rural areas. However the aged population of over 60 years old in rural areas is higher than that in urban and these aged population prefer more oriental medical care than the other age group. Therefore, the government planned to carry out the oriental medical care demonstration project in a designated rural areas in 1990. The study was carried out to find out the utilization pattern of medical care and consumers attitude toward oriental medical care treatment provided by health centers. The interview survey was applied to collect the data and 187 patients, who visited to health centers to receive care in 1991, were selected by random sampling. The study results obtained were as follows : 1) Among the 187 respondents, male was 31.6% and female, 68.4%. 2) 73.8% of the respondents were the age of over 45 years old. 3) For the motivation of visiting the health center to receive oriental medical care, 37.4% of the respondents visited purposely according to announcement of oriental medical care and 26.2% of them made a decision by themself 20.3% of them was recommended by the neighbors. 4) The most frequent symptoms surveyed were the disease of the musculoskeletal system and connective tissue. 5) By the subjective judgement of the respondents from the result of the oriental medical treatment, recovered or improved cases represent 69.5%. It is considered that the oriental medical care was acceptable, and also the respondents were satisfied with the oriental medical care in terms of kindness of oriental medical doctors, treatment time and expenses of care.

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Case Report of Polyarteritis Nodosa Treated with Oriental Treatments Including Acupotomy (침도침 시술을 가미한 복합한방치료를 시행한 결절성 다발 동맥염의 치험례)

  • Lee, Eun-Sol;Kam, Chul-Woo;Youn, Hyoun-Min;Jang, Kyung-Jeon;Song, Chun-Ho;Kim, Young-Kyun;Kim, Cheol-Hong
    • Journal of Acupuncture Research
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    • v.29 no.3
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    • pp.129-137
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    • 2012
  • Objectives : Polyarteritis nodosa is a progressive disease of connective tissue that is characterized by nodules along arteries; nodules may block the artery and result in inadequate circulation to the particular area. This report is intended to estimate the efficacy using oriental complex treatment on a patient with Polyarteritis nodosa. Materials and Methods : From 19th March, 2012 to 12th May, 2012, One male inpatient diagnosed with polyarteritis nodosa was treated with general oriental medicine therapy : needle-embedding therapy ; acupuncture ; pharmacopuncture ; acupotomy therapy and herbal medication. VAS(visual analogue scale) was used for evaluation of both leg pain. Other subjective symptoms including night sweat, tinnitus, upper heat were evaluated by percentage comparing the symtoms before and after treatment. Results : The patient showed a certain degree of improvement in both leg pain and other subjective symtoms. Conclusions : Oriental treatments such as needle-Embedding therapy, acupuncture and moxibustion therapy, pharmacopuncture therapy, acupotomy therapy and herbal medication can be effective for controlling pain and other accompanied symtoms due to polyarteritis nodosa.

Epidemiological study on prevalence of fascioliasis in slaughtered cattle (도축 소의 간질 감염실태에 관한 역학적 고찰)

  • Kim, Yong-Hwan;Park, Jong-Tae;Kim, Jae-Ik;Kim, Hyun-Joong;Kim, Tae-Soon;Jang, Mi-Sun;Kim, Jeong-Nam;Park, Duk-Woong;Bae, Seong-Yeol;Park, Ja-Yoon;Seo, Eun-Ju
    • Korean Journal of Veterinary Service
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    • v.31 no.2
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    • pp.207-217
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    • 2008
  • This study was carried out to investigate the prevalence of fascioliasis of slaughtered and farmed cattle in Gwangju area from February to November in 2007. A total of 1,000 cattle fecal samples were collected from slaughter houses (n=805) and farms (n=185). Twelve (1.2%) were found as positive cases with excretion of the egg of Fasciola spp in the fecal specimens, and 128 (12.8%) were positive in intestinal parasitism using the flotation and sedimentation procedures. The infection rate of fluke larvae from the slaughtered cattle at abattoirs in Gwangju was 0.75% (6 out of 805 heads). In histopathology, there were several liver lesions such as inflammation with infiltration of eosinophil, polymorphonuclear cells, mononuclear cells and multinucleated giant cells, proliferation of connective tissue, calcification and abscess formation.

The efficacy of dexamethasone injection on postoperative pain in lower third molar surgery

  • Latt, Maung Maung;Kiattavorncharoen, Sirichai;Boonsiriseth, Kiatanant;Pairuchvej, Verasak;Wongsirichat, Natthamet
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.16 no.2
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    • pp.95-102
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    • 2016
  • Background: Surgery on the lower impacted third molar usually involves trauma in the highly vascularized loose connective tissue area, leading to inflammatory sequelae including postoperative pain, swelling, and general oral dysfunction during the immediate post-operative phase. This study aimed to investigate the effectiveness of preoperative injection of a single dose of 8 mg dexamethasone for postoperative pain control in lower third molar surgery. Methods: A controlled, randomized, split-mouth, prospective study involving lower third molar surgery was performed in 31 patients. The randomized sampling group was preoperatively injected, after local anesthesia, with a single dose of dexamethasone (8 mg in 2 ml) through the pterygomandibular space; 2 ml of normal saline (with no dexamethasone) was injected as a placebo. Results: The pain VAS score was significantly different on the day of the operation compared to the first post-operative day (P = 0.00 and 0.01, respectively), but it was not significantly different on the third and seventh postoperative day between the control and study groups. There was a significant reduction in swelling on the second postoperative day, and a difference between the second postoperative day and baseline value in the study group (P < 0.05). Trismus was highly significantly different on the second postoperative day and between baseline and second postoperative day between the groups (P = 0.04 and 0.02, respectively). Descriptive statistics and independent-samples t- test were used to assess the significance of differences. Conclusions: Injection of 8 mg dexamethasone into the pterygomandibular space effectively reduced the postoperative pain and other postoperative sequalae.

Identification of immunological parameters associated with the alveolar bone level in periodontal patients

  • Park, Chang-Seo;Lee, Ju-Yeon;Kim, Sung-Jo;Choi, Jeom-Il
    • Journal of Periodontal and Implant Science
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    • v.40 no.2
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    • pp.61-68
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    • 2010
  • Purpose: The present study was performed to clarify the relationship between periodontal disease severity and selected immunological parameters consisting of serum IgG titer against periodontopathogenic bacteria, the expression of the helper T-cell cytokine by gingival mononuclear cells, and patients' immunoreactivity to cross-reactive heat shock protein (HSP) epitope peptide from P. gingivalis HSP60. Methods: Twenty-five patients with moderate periodontitis had their gingival connective tissue harvested of gingival mononuclear cells during an open flap debridement procedure and peripheral blood was drawn by venipuncture to collect serum. The mean level of interproximal alveolar bone was calculated to be used as an index for periodontal disease severity for a given patient. Each of selected immunologic parameters was subject to statistical management to seek their correlations with the severity of periodontal disease. Results: A significant correlation could not be identified between serum IgG titers against specific bacteria (Porphyromonas gingivalis, Prevotella intermedia, Fusobacterium nucleatum, Actinobacillus actinomycetemcomitans, and Streptococcus mutans) and the severity of periodontal disease. Expression of interleukin (IL)-10 by gingival mononuclear cells was statistically significant in the group of patients who had higher levels of alveolar bone height. However, a similar correlation could not be demonstrated in cases for IL-4 or interferon-$\gamma$. Patients' serum reactivity to cross-reactive epitope peptide showed a significant correlation with the amount of alveolar bone. Conclusions: It was concluded that expression of IL-10 by gingival mononuclear cells and patients' sero-reactivity to the cross-reactive HSP peptide of P. gingivalis HSP60 were significantly correlated with alveolar bone height.

Histometrical evaluation of biphasic calcium phosphate in surgically created 1-wall periodontal intrabony defects in dogs (성견 일벽성 치주 결손부에 이식한 biphasic calcium phosphate의 조직계측학적 평가)

  • Yon, Je-Young;Kim, Dong-Jin;Hong, Sung-Bae;Hong, Ji-Yeon;Kim, Sung-Tae;Lee, Yong-Ho;Cho, Kyu-Sung;Kim, Chong-Kwan;Choi, Seong-Ho
    • Journal of Periodontal and Implant Science
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    • v.38 no.2
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    • pp.171-178
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    • 2008
  • Purpose: The aim of this study was to evaluated biphasic calcium phosphate applied in surgically created 1-wall periodontal intrabony defects in dogs by histometrical analysis. Material and Method: Critical sized($4\;mm\;{\times}\;4\;mm$), one wall periodontal intrabony defects were surgically produced at the proximal aspect of mandibular premolars in either right and left jaw quadrants in four canines. The control group was treated with debridement alone, and experimental group was treated with debridement and biphasic calcium phosphate application. The healing processes were histologically and histometrically observed after 8 weeks. Results: In biphasic calcium phosphate group, more new bone and cementum formation, less epithelium and connective tissue attachment were observed compared to other groups. But there was no statistical significance. Conclusion: Though the statistically significant difference could not be found, it seemed that there was more new bone and cementum formation with applying biphasic calcium phosphate in 1 wall intrabony defects in dogs by preventing junctional epithelium migration.