• Title/Summary/Keyword: confocal scanning

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Application of Confocal Laser Scanning Microscopy and Fiber Distribution Index to Study Kenaf Handsheet Properties

  • Pang, Myong-Hyeok;Park, Jong-Moon;Cho, Nam-Seok
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.31 no.3
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    • pp.54-59
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    • 1999
  • This study was to quantify fiber distributions in thickness direction of kenaf handsheets as a fiber distribution index (FDI) and to analyze the relationship between FDI and the handsheet properties. The images of fiber distribution in z-direction were obtained by Confocal Laser Scanning Microscope (CLSM) and analyzed by image analysis technique. The proposed FDI had a good correlation with high R2 vlaues with various properties of paper, such as apparent density, scattering coefficient , burst index, tear index, tensile index, and folding endurance. The proposed FDI was shown as a good index to quantify paper properties.

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Indented Surface Configuration and its Volume Calculation (압입 표면형상의 구현과 체적계산법)

  • Yang, Jae-Yong;Yoon, Moon-Chul
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.21 no.5
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    • pp.708-713
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    • 2012
  • The indented geometry for rockwell hardness indenter has been configured by using Confocal Laser Scanning Microscopy (CLSM). For this purpose, the CLSM can be well suited to construct the three-dimensional indented volume from the indented surface by rockwell hardness tester. Furthermore, the height data of HEI(height encoded image) by CLSM must be acquired at first and converted to indented surface later. And the indented surface patterns enable us to predict the indenter shape and volume. This volume can be used to study the rockwell hardness model as a volume parameter. As a result, the technique performed in this study by combining the CLSM with compensation technique is an excellent one to obtain the geometries of indented surfaces over a wide range of surface resolution in a micro scale. And it can be used for micro volume calculation.

기하양상을 고려한 균열 내 투수계수 산정

  • ;Ichikawa Y.
    • Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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    • 2004.09a
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    • pp.159-163
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    • 2004
  • This study was conducted to calculate the permeability coefficient in a single fracture while taking the true fracture geometry into consideration. The fracture geometry was measured using the confocal laser scanning microscope (CLSM). The CLSM geometry data were used to reconstruct a fracture model for numerical analysis using a homogenization analysis (HA) method. The HA is a new type of perturbation theory developed to characterize the behavior of a micro-inhomogeneous material that involves periodic microstructures. The HA permeability was calculated based on the local geometry and local material properties (water viscosity in this case). The results show that the permeability coefficients do not follow the theoretical relationship of the cubic law.

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Reflection-type Optical Waveguide Index Profiling Technique

  • Youk YoungChun;Kim Dug Young
    • Journal of the Optical Society of Korea
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    • v.9 no.2
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    • pp.49-53
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    • 2005
  • We report a new configuration of a reflection-type confocal scanning optical microscope system for measuring the refractive index profile of an optical waveguide. Several improvements on the earlier design are proposed; a light emitting diode (LED) at 650 nm wavelength instead of a laser diode (LD) or He-Ne laser is used as a light source for better index precision, and a simple longitudinal linear scanning and curve fitting techniques are adapted instead of a servo control for maintaining an optical confocal arrangement. We have obtained spatial resolution of 700 nm and an index precision of $2\times10^{-4}$. To verify the system's capability, the refractive index profiles of a conventional multimode fiber and a home-made four-mode fiber were examined with our proposed measurement method.

Research on Reconstruction Technology of Biofilm Surface Based on Image Stacking

  • Zhao, Yuyang;Tao, Xueheng;Lee, Eung-Joo
    • Journal of Korea Multimedia Society
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    • v.24 no.11
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    • pp.1472-1480
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    • 2021
  • Image stacking technique is one of the key techniques for complex surface reconstruction. The process includes sample collection, image processing, algorithm editing, surface reconstruction, and finally reaching reliable conclusions. Since this experiment is based on laser scanning confocal microscope to collect the original contour information of the sample, it is necessary to briefly introduce the relevant principle and operation method of laser scanning confocal microscope. After that, the original image is collected and processed, and the data is expanded by interpolation method. Meanwhile, several methods of surface reconstruction are listed. After comparing the advantages and disadvantages of each method, one-dimensional interpolation and volume rendering are finally used to reconstruct the 3D model. The experimental results show that the final 3d surface modeling is more consistent with the appearance information of the original samples. At the same time, the algorithm is simple and easy to understand, strong operability, and can meet the requirements of surface reconstruction of different types of samples.

The effect of using nanoparticles in bioactive glass on its antimicrobial properties

  • Maram Farouk Obeid;Kareim Moustafa El-Batouty;Mohammed Aslam
    • Restorative Dentistry and Endodontics
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    • v.46 no.4
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    • pp.58.1-58.8
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    • 2021
  • Objectives: This study addresses the effect of using nanoparticles (np) on the antimicrobial properties of bioactive glass (BAG) when used in intracanal medicaments against Enterococcus faecalis (E. faecalis) biofilms. Materials and Methods: E. faecalis biofilms, grown inside 90 root canals for 21 days, were randomly divided into 4 groups according to the antimicrobial regimen followed (n = 20; BAG-np, BAG, calcium hydroxide [CaOH], and saline). After 1 week, residual live bacteria were quantified in terms of colony-forming units (CFU), while dead bacteria were assessed with a confocal laser scanning microscope. Results: Although there was a statistically significant decrease in the mean CFU value among all groups, the nano-group performed the best. The highest percentage of dead bacteria was detected in the BAG-np group, with a significant difference from the BAG group. Conclusions: The reduction of particle size and use of a nano-form of BAG improved the antimicrobial properties of the intracanal treatment of E. faecalis biofilms