• Microbiology and Biotechnology Letters
• /
• v.15 no.2
• /
• pp.84-88
• /
• 1987

Poly(ethylene glycol)-PPB two phase system was used tot the purification of $\beta$-galactosidase from Lactobacillus sporogenes. The smaller the molecular weight of concentration of PEG phase in-creased, proteins as well as $\beta$-galactosidase was partitioned into the top phase. All cell debris were confined to the potassium phosphate phase (bottom phase), approached to the binodial line. The purification ratio increased by changing the polymer-salt composition of the tie line towards higher salt concentrations. It was also possible to obtain higher purification of the enzyme after two-step extraction using PEG 1000 and PEG 300. The top phase contained 74% of the total $\beta$-galactosidase with a purification factor of 2.1.

• Journal of the Korea Concrete Institute
• /
• v.20 no.1
• /
• pp.59-66
• /
• 2008

This study is about manufacturing and producing girder, which is an essential component of bridge structure, in a composite of FRP + concrete. This has a higher competitive power in price than steel girder. The girder used in this study is made of glass fiber which has a lower elastic modulus than steel and thus has some technical limitations such as excessive deflection compared to steel girder and lack of production facilities in FRP production companies to make a large-section component material. Thus, this study suggested a section of a new module that will allow for applying a large section in order to solve the technical difficulties mentioned above and to secure low stiffness of FRP, developed a new FRP+concrete composite girder that is filled with the appropriate amount of concrete. To identify the structural behavior of this FRP+concrete composite girder, experiments were conducted to measure its flexural strength according to the difference in the strength of confined concrete and the existence of stud. The results of the flexural strength test confirmed the composite effect from confining concrete and the effect of increase in strength proportional to the strength of concrete. In developing FRP+concrete composite girder, NDT study was also conducted to analyze the interface characteristics of concrete and FRP.

• Journal of the Korea Concrete Institute
• /
• v.19 no.1
• /
• pp.121-130
• /
• 2007

To investigate the enhancement in strength and deformation capacities of concrete confined by FRP composites, tests under axial loads were carried out on three groups of thirty six short columns in circular section with diverse GFRP confining reinforcement. The major test variables considered include fiber content or orientation, wrap or tube type by varying the end loading condition, and continuous or discontinuous confinement depending on the presence of vortical spices between its two halves. The circumferential FRP strains at failure for different types of confinements were also investigated with emphasis. Various analytical models capable of predicting the ultimate strength and strain of the confined concrete were examined by comparing to observed results. Tests results showed that FRP wraps or tubes provide the substantial increase in strength and deformation, while partial wraps comprising the vertical discontinuities fail in an explosive manner with less increase in strength, particularly in deformation. A bilinear stress-strain response was observed throughout all tests with some variations of strain hardening. The failure hoop strains measured on the FRP surface were less than those obtained from the tensile coupons in all tests with a high degree of variation. In overall, existing predictive equations overestimated ultimate strengths and strains observed in present tests, with a much larger scatter related to the latter. For more accuracy, two simple design- oriented equations correlated with present tests are proposed. The strength equation was derived using the Mohr-Coulomb failure criterion, whereas the strain equation was based on entirely fitting of test data including the unconfined concrete strength as one of governing factors.