• Asian-Australasian Journal of Animal Sciences
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• v.29 no.12
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• pp.1734-1741
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• 2016

This study was conducted to examine the effects of adding phytoncide oil extracted from Korean pine nut cone by-product to the diet of dairy cows on milk yield and compositions, fatty acid characteristics, complete blood count and stress response. A total of 74 Holstein cows were used for 30 days and divided into two groups. Each group was given a basal diet (C) or an experimental diet containing phytoncide additives at 0.016% (T) in feed. The results showed that phytoncide feeding had no effect on milk yield. In addition, there were no observed effects on milk composition, but the ratio of fatty acid in milk was significantly affected by the phytoncide diet, and it showed a positive effect. Not only were the major functional fatty acids, conjugated linoleic acid and eicosapentaenoic acid increased, but also ${\omega}6:{\omega}3$ fatty acid ratio was reduced in milk of T group (p<0.05). In blood analysis, the complete blood count showed no significant difference between C and T group on all parameters. However, the cortisol concentration was significantly decreased in T group compared to control (p<0.05). Taken together, we suggest that phytoncide oil does not have a great influence on the physiological changes, but can be a potential feed additive that improves the milk fatty acid and stress resilience in dairy cows. In addition, it will contribute to the development of feed resource, a reduction in feed cost and a lessening of environmental pollution.

• The Journal of Korean Medicine
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• v.25 no.3
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• pp.123-136
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• 2004

Objectives : The purpose of this investigation is to evaluate the effects of Woohwangcheongsim-won (WC) on the in vitro neuronal development and alteration in gene expression in a hypoxia model using cultured rat cortical cells. Methods : E/sub 18/ rat cortical cells were grown in a neurobasal medium containing B27 supplement and various concentration of WC. Initial development of growth cone was investigated by phase-contrast microscopy, while dendritic spine formation and synaptogenesis were investigated by immunocytochemistry with SynGAPα(a postsynaptic marker) and synaptophysin (presynaptic marker) antibodies. Alteration in gene expression was analyses by microarray using rat 5K-TwinChips. Results : WC suppressed the development of growth cones and WC increased the number of dendritic spines at 20 and 50㎍/mL concentration but there was no statistical significance. Instead, it significantly decreased the number at 100㎍/mL. The expression of anti-apoptosis gene Bcl2-like 1 (Bcl211) increased (Global M=0.46), while Akt1 decreased. Proapoptosis genes Bad and PDCD2 increased. The expression of hemoglobin alpha 1 (probably neuroglobin) increased (Global M=0.93). The expression of antioxidants such as catalase, heme oxygenase (HO), and PRKAG2 gene increased. The expression PKC gene increased. The expression of retinoic acid receptor alpha (RARα) increased significantly (Global M=1.0). Conclusions : These data suggest that WC trends to suppress cellular activity slightly in normoxia and increases the expression of apoptosis-, antioxidation-, oxygen capture-related genes in hypoxia, but increases Bcl111 that anti-apoptosis gene, on the other hand increases Bad, PDCD2 that pro-apoptosis genes, too..

• Journal of Korean Society of Forest Science
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• v.69 no.1
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• pp.19-27
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• 1985

Introgressive hybridization of Pinus densiflora in Korea was investigated by examining the position of resin ducts in 4,992 trees from 67 populations. Judging that P. densiflora has only external resin ducts, P. thunbergii only medial resin ducts, and any tree which has even one needle with external and medial resin ducts is hybrid, hybrids are found at all populations investigated. As variation in position of resin ducts was found among needles on a tree, the number of needles investigated from a tree affects judgment of the tree's hybridity. Therefore, hybridity seems to be judged by many traits rather than by the single trait, position of resin ducts. Seed production by artificial pollination between P. densiflora and P. thunbergii was very poor, i.e. one full seed from five female flowers or from two mature cones. This poor seed production, in addition to the low hybrid vigor, if any, seems to make the artificial pollination between the two species for mass-production of seeds for planting impractical.

• Journal of Korean Society of Forest Science
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• v.62 no.1
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• pp.82-86
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• 1983

Cone and seed development after self-pollination in 14-year-old Pinus rigida Mill. was investigated in an $F_1$-hybrid seed orchard that consists of Pinus rigida Mill. and Pinus taeda L. in alternative rows. On an average, 83.8% of the self-pollinated receptive female strobili developed into cones. This value appears to be normal compared to cone development after open-pollination in other pine species such as in Pinus sylvestris L. However, there was a great variation in filled seed development after self-pollination among the investigated individual trees (Table 1-1). The 34.5%, a ratio of filled seeds after self-pollination was significantly lower than 91.7%, that by the open-pollination. Some of the investigated trees developed 70.0%-83.5% of filled seeds after self-pollination. Thus, those are not desirable as trees for seed parent in establishing $F_1$-hybrid seed orchards in future. A further study on the rate of filled seed development by various pollination systems consisting of self-pollination, self-and cross-pollination with intraspecific, mixed (tree's own and foreign pollens) pollens and cross-pollination with interspecific, mixed pollens of the two parental tree species is necessary to determine the quality of seed parent trees in establishing $F_1$-hybrid seed orchards.

• Journal of Korean Society of Forest Science
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• v.65 no.1
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• pp.24-30
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• 1984

The average number of empty seeds per cone on the mature tree was $10.03{\pm}0.61$. The ratios of empty seeds to total seeds and to full seeds were $7.38{\pm}0.35%$ and $8.42{\pm}0.49%$, respectively in average. The number of empty seeds per cone and the ratios were varied with the site condition; fruited year, and individual tree. The contribution rates of these sources of variance to the number of empty seeds per cone were respectively 3.50%, 8.06%, and 55.41%. No correlationships were found between the amount of empty seeds in number or ratio and the cone production, and the D.B.H. of mother tree. However, both the number of empty seeds and the ratio of empty seeds to total seeds showed significant positive correlations with the number of 1-year-old cones per tree.

• Economic and Environmental Geology
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• v.19 no.1
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• pp.25-33
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• 1986

The geology of the Jeju island is characterized by the occurrence of thick voluminous basaltic lava flows and minor trachytic lavas. The land form can be divided topographically into the lava plateau, the shield-shaped Halla volcanic edifice and the parasitic cones whose number is more than 360, which is closely related to the cyclic volcanism of the island. Especially, the trachytic lavas seem to be the latest differentiation products of the cyclic activities and largely classified into two groups (Backlokdam group and Sanbangsan group) on the basis of the occurrence and the petrography. The paleomagnetism and the radiometric age on the two groups of trachyte suggest a lower and an upper time limits of the volcanic stratigraphy of the island. An average age of the trachyte of the Sanbangsan group is $0.733{\pm}0.056m.y.$, and is correlated to a horizon a little lower than the boundary (0.69m.y.) between the Brunches normal epoch and Matuyama reversed epoch. An average age of $0.025{\pm}0.008m.y.$ determined from the Backlokdam trachyte may be corrected to anyone horizon of the Laschamp, or the Lake Biwa, or the Lake Mono excursion. The two groups of trachytes are distributed with the latitude difference of 9'($0.15^{\circ}$), and with age difference of about 0.71 m.y. Assuming that the two trachyte groups were erupted from the same stationary hot spot, the lithosphere comprising the Jeju island is considered to have moved southward with a rate of about 2.3 cm/year.

• Journal of the Korean Magnetics Society
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• v.26 no.6
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• pp.213-218
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• 2016

This study reviewed mineral composition on Scoria samples of this area, atomic value state of oxidized steel, and magnetic property in order to look into characteristics of scoria that was distributed in southern area of mountainous areas, Halla Mt. of Jeju Island. By XRD analysis, mineral composition was confirmed, and characteristics of iron compounds existed in samples were investigated through $M{\ddot{o}}ssbauer$ spectroscope. Composing minerals could be learnt as feldspar basalt from XRD analysis because composting minerals were composed of quartz and feldspar anorite mainly, and iron compounds were made up with olivine, pyroxene, ilmenite, hematite, and magnetite. By $M{\ddot{o}}ssbauer$ spectroscope analysis on these iron compounds. it consisted of hematite and magnetite which showed hyperfine magnetic field of sextet mostly, and also doublet by olivine, pyroxene, ilmenite could be seen as appearing together. As a result of comparing with samples of Jeju western area having been announced in previous research, I.S. and Q.S. values of olivine, $Fe^{2+}$, were 122 mm/s and 3.09~3.13 mm/s respectively, and a fact could be known that $Fe^{2+}$ olivine having similar structure each other was contained, and the ratio of $Fe^{3+}/Fe_{tot.}$. was 85.90~92.82 %. From these findings, it was able to be presumed that they belonged to samples having been formed on the land at the same period of time. As a result of investigating area ratio of tetrahedron (A site) and octahedron (B site) regarding magnetite in samples, it was turn out to be 0.22~0.55 less than 2.

• Geophysics and Geophysical Exploration
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• v.12 no.1
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• pp.1-7
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• 2009

Airborne electromagnetics (AEM) is a useful tool for investigating volcanic structures because it can survey large and inaccessible areas. Disadvantages include lower accuracy and limited depth of investigation. The Grounded Electrical Source Airborne Transient Electromagnetic(GREATEM)survey system was developed to increase the depth of investigation possible using AEM. The method was tested in a survey at Mount Bandai in north-eastern Japan. Mount Bandai is an andesitic stratovolcano that rises 1819m above sea level. An eruption in July 1888 left a hoof-shaped collapsed wall in its northern crater and avalanche debris at its base. Previous surveys of Mount Bandai allow for comparisons of data on its structure and collapse mechanism as obtained by GREATEM and other geophysical methods. The results show resistive structures in recent volcanic cones and conductive structures in the collapsed-crater area. Conductive areas around the collapsed wall correspond to an alteration zone resulting from hydrothermal activity, supporting the contention that a major cause of the collapse associated with the 1888 eruption was hydrothermal alteration that structurally weakened the interior of the volcanic edifice.

• Journal of Korean Society of Forest Science
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• v.33 no.1
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• pp.59-67
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• 1977

Seasonal changes in free arginine concentration in Pinus radiata buds were studied in four clones from May, 1974 to June, 1975. The buds were from the tips of first order branches which had flowered the previous year, and the significant findings were as follows: (1) In general, arginine concentration in the buds of the four clones showed the highest peaks from December to March, a period spanning the time of flower initiation, and the lowest levels in Angust and September. (2) The timing of individual arginine peaks during the period of flower initiation matched roughly the timing of initiation of several female cone clusters, as determined from Clone 7 which characteristically initiates three cycles of female cones on its leading shoot. (3) The heavy-flowering clones showed higher arginine concentration than ther poorer flowering ones, especially at the time of flower initiation.

• Journal of Korean Society of Forest Science
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• v.78 no.3
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• pp.323-330
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• 1989

Pinass densiflora natural forests of four local forms in Korea were studies to investigate effective biomass estimation method. Dimension analysis was used and three allometric regression models, such as logWt=A+BlogD, logWt=$A+B1ogD^2H$ and 1ogWt=A+BlogD+ClogH were applied to estimate biomass, The most accurate estimation was made by the regression model of logWt=A+BlogD+ClogH where Wt is dry weight, D is diameter at breast height, and H is tree height. However, dry weights of cones and dead branches were remotely related to tree size factor, such as D and H. In the interest of practical use. generalized allometric regressions for all samples trees of four stands were computed and analysis of covariance was used to compare the allometric regressions among the four stands. Based on the test criteria applied in this study, significant differences were found in terms of error variance and regression intercept, not in terms of regression slope. These trends suggest a generalized biomass regression is not valid for accurate estimation over a range of four local form stands.