• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.1
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• pp.115-121
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• 2011

This study was performed to investigate the antioxidant activity of extracts of black Panax ginseng (BGE) and its crude saponin (BGECS). The antioxidant activities of BGE and BGECS were evaluated for free radical scavenging activity against stable free radical (1,1-diphenyl-2-picrylhydrazyl) DPPH, nitrite, hydrogen peroxide and superoxide. In addition, the antioxidant activity of BGE and BGECS against peroxyl radicals, hydroxyl radicals and peroxynitrites were determined by the total oxy-radical scavenging capacity (TOSC) assay. As a result, BGE and BGECS were found to have a strong inhibitory activity with >90% against the DPPH radical at $1000{\mu}g/m{\ell}$ concentrations. Also, BGE and BGECS exhibited strong inhibitory activity with >80% against hydrogen peroxide at lower concentration ($125{\mu}g/m{\ell}$). Moreover, specific TOSC values (405 and 473 TOSC/mM) of BGE and BGECS against peroxynitrites were higher than GSH (347 TOSC/mM) used a positive control. These results suggest that BGE and BGECS could be useful to develop functional foods against disease related oxidative stress.

• Journal of Microbiology and Biotechnology
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• v.29 no.10
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• pp.1561-1569
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• 2019

Curcumin, the major bioactive constituent of turmeric, has been reported to have a wide range of pharmacological benefits; however, the low solubility in water has restricted its systemic bioavailability and therapeutic potential. Therefore, in the current study, we aimed to investigate the effect of turmeric fermentation on its curcumin content and anti-inflammatory activity by using several lactic acid bacteria. Fermentation with Lactobacillus fermentum significantly increased the curcumin content by 9.76% while showing no cytotoxicity in RAW 246.7 cells, as compared to the unfermented turmeric, regardless of the concentration of L. fermentum-fermented turmeric. The L. fermentum-fermented turmeric also promoted cell survival; a significantly higher number of viable cells in lipopolysaccharide (LPS)-induced RAW 264.7 cells were observed as compared to those treated with unfermented turmeric. It also displayed promising DPPH scavenging ($7.88{\pm}3.36%$) and anti-inflammatory activities by significantly reducing the nitrite level and suppressing the expression of the pro-apoptotic tumor necrosis factor-alpha and Toll-like receptor-4 in LPS-induced RAW 264.7 cells. Western blot analysis further revealed that the anti-inflammatory activity of the fermented turmeric was exerted through suppression of the c-Jun N-terminal kinase signal pathway, but not in unfermented turmeric. Taken together, the results suggested that fermentation with lactic acid bacteria increases the curcumin content of turmeric without increasing its cytotoxicity, while strengthening the specific pharmacological activity, thus, highlighting its potential application as a functional food ingredient.

• KSBB Journal
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• v.29 no.2
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• pp.92-97
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• 2014

In this study, we investigate the antimicrobial and antioxidant activities of extracts of Enteromorpha intestinalis. To evaluate the antimicrobial and antioxidant activities, three solvents (hexane, chloroform and methanol) were applied to obtain extracts. The extraction yields are hexane (A) 1.11%, chloroform (B) 0.94%, and methanol (C) 8.2%. Also, the contents of total phenolic compounds of extract A, B, C are 4.03%, 8.15%, and 2.33%, respectively. In the results of antimicrobial activity, extract A and B have higher activity than that of extract C. Especially, Vibrio vulificus, Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli are more sensitive than others. In antioxidant activity, extract A and B present the higher DPPH activity than that of control (BHA and ascorbic acid). The DPPH radical scavenging activity and SOD-like activity are ordered as B>C>A. However, extract C show high value in ferric reducing antioxidant power assay. In nitrite scavenging activity, extract A is relatively higher than others. However, the antioxidant activities are lower than that of controls (ascorbic acid and BHA). The antioxidant activities are presented the increasing pattern of increasing by the increasing of extract concentration.

• Textile Coloration and Finishing
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• v.27 no.4
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• pp.229-244
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• 2015

The effects of synthesis conditions on characteristics of the calcium-azo complex pigment, C.I. Pigment Red 57:1, were studied. It was mainly considered that the industrially required synthesis conditions for lowering electrical conductivity of the pigment solution keeping pigment quality such as particle size and color characteristics. Three parameters were chosen as control factors during the synthesis. The first was the amount of hydrochloric acid added to transform sodium nitrite into nitrous acid. The second was the amount of calcium chloride added to insolubilize the synthesized azo dye. The final factor was pH control during the coupling reaction. The electrical conductivity and pigment aggregate particle size were dependent on the amount of hydrochloric acid and calcium chloride. Higher HCl concentration gave brighter yellowish-red color because of smaller particle aggregate size and narrower size distribution. Amount of charged ions in the synthesis process might affect the "lake" formation resulting different particle aggregate size and color shade.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.40 no.4
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• pp.193-200
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• 2007

Ion-exchange chromatography and ultra-filtration permeation were used to extract carnosine from the eel Anguilla japonica. In an investigation of its antioxidant properties, the eel carnosine prevented lipid peroxidation in linoleic acid systems, scavenged free radicals, and exhibited superoxide dismutase-like activity. These activities increased as the carnosine concentration increased. The nitrite scavenging effects (NSEs) of commercial carnosine and the eel carnosine were measured at various acidic pHs (1.2, 3.0, and 4.2). For both types of carnosine, the maximum NSE was observed at pH 1.2. At this pH, the NSE of the eel carnosine was 65.3%. Both types of carnosine were effective at maintaining reasonably good color of ground beef patties over 5 days of storage at $4^{\circ}C$ and inhibited metmyoglobin formation as well as lipid peroxidation. These data suggest that the eel carnosine might be useful as a "natural" antioxidant in commercial production and storage of muscle foodstuffs.

• Journal of Life Science
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• v.10 no.1
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• pp.52-60
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• 2000

Up to now, there have been done much efforts in regard to nitrate reductase activity (NRA) of dicotyledonous herbs and important crop monocotyledons, but few to wild plants having canopy structure such as Carex. The objective of the present study are to determine: a) the optimum in vivo NR assay conditions for leaf samples of Carex species, b) changes of NRA according to section within leaf and leaf ages, c) diurnal variations. Optimized assay media of each Carex species were determined. NRA of C. rostrata adapted to oligotrophic habitats is readily saturated at lower substrate concentration than those of C. distans and C. gracilis, adapted to meso- and eutrophic habitats, respectively. All Carex species investigated have higher NRA in leaves than in roots. NRA of all species showed maximal values at the middle section of each leaf and in the youngest fully expanded leaves. Compared to C. gracilis, NR in leaves of C. distans was adapted readily to the light period. On the whole, Carex showed rather delayed diurnal variation. Even if the in vivo nitrate reductase assay based on nitrite estimation does not give an accurate estimation of total nitrate reduced, it still serves as a useful tool to find out relative differences in varying environmental conditions. Additionally, in vivo RNA measurements are helpful to understand nitrate reduction and basic nitrogen metabolism of Carex species having different canopy structure.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.202.4-203
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• 2003

The present study was carried out to clarify whether isoflavonoids isolated from Belamcandae Rhizoma (Iridaceae) inhibit inflammation and angiogenesis by the experimental methods in vitro and in vivo. Among the isolated isoflavonoids, such as irigenin, irisflorentine, and iristectorene B inhibited nitric oxide (NO) production, as measured by nitrite formation at 3-30 ${\mu}M$. Also these compounds reduced cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) enzyme expression in a concentration dependent manner, when measured by western blotting, at 3-30 ${\mu}M$. Irigenin, irisflorentine and iristectoren B decreased angiogenesis of chick embryos in the chorioallantoic membrane assay. These compounds also reduced the proliferation of calf pulmonary arterial endothelial (CPAE) cells and found to possess relatively weak gelatinase/collagenase inhibitory activity in vitro. These compounds, when administered subcataneously at the dose of 30mg/kg for 20 days to mice implanted with murine Lewis lung carcinoma (LLC), caused a significant inhibition of tumor volume. Therefore, antiangiogenic activities of isoflavonoids from Belamcandae Rhizoma might be due to antiproliferative activities under inhibition the induction of COX-2 and iNOS enzyme.

• Journal of Sensor Science and Technology
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• v.32 no.2
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• pp.67-74
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• 2023

A microfluidic paper-based analytical device (µPAD) is proposed for the selective detection of ammonia in water by using the modified Berthelot reagent and a fluidic channel consisting of hollow paper. The modified Berthelot reagents were uniformly dispersed in cyclohexane and then immobilized in a detection zone of the µPAD. The loading position of the reagents and the type of a sample flow channel were optimized to achieve a sensitive ammonia detection within a short analytical time. The NH₃ µPAD exhibits a linear colorimetric response to the concentration of ammonia dissolved in water in the range of 1-100 mg L^-1, and its limit-of-detection is 1.75 mg L^-1. In addition, the colorimetric response was not influenced by the addition of 100 mg L^-1 nitrogen containing compounds (sodium nitrate, sodium nitrite, uric acid, hydroxylamine, butylamine, diethylamine) or inorganic salts (NaCl, Na₂HPO₄), presenting the enough selectivity in the detection of water-dissolved ammonia against possible interferents.

• Biotechnology and Bioprocess Engineering:BBE
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• v.8 no.6
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• pp.368-372
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• 2003

The growth and amino acid contents of the cyanobacterium, Spirulina platensis strain NIES 46, were investigated using ammonium, nitrate, nitrite, or urea as the sole nitrogen source in a batch culture. Chlorophyll a concentration was highest at 2,096$\mu\textrm{g}$/L in the nitrate group after 10days of cultivation, while the dry weight of S. platensis was highest at 4.5g/L in the ammonium group after 30days of cultivation. The total amino acid content was highest at 174mg/g dry weight of S. platensis in the urea group at the end of the cultivation period, yet the amino acid patterns for S. platensis were similar for all the experimental groups. Therefore, it seemed that the growth and amino acid composition of S. platensis varied depending on the type of nitrogen sources, while the amino acid patterns were not changed. Also, the most efficient harvesting time for S. platensis seemed to be approximately 10 days after cultivation.

• Korean Journal of Microbiology
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• v.11 no.3
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• pp.121-128
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• 1973

In order to clarify the best cultural conditions of Rhizopus niveus the effects of aeration, pH and various nutrients, such as different carbon and nitrogen sources, vitamins, and growth substances, on the mycelial growth were studied through liquid culture, and amylase activities of the fungus at different cultural periods were measured. Soluble starch, xylose and galactose are excellent sources of carbon for growth of the fungus. Sorbose and lactose are utilized slightly for growth. peptone, ammonium sulfate and alanine are excellent nitrogen sources for growth, tyrptophane nad potassium nitrate are utilized slightly for growth and sodium nitrite is not utilized. Thiamine nad gibberellin are excellent growth substances for the fungal growth, and biotin, nicotinamide and indole acetic acid (IAA) are also effective. Rhizopus niveus grows better at rotatory culture than at stationary culture and earlier growth of the fungus increases remarkably at rotatory culture. Optimum pH than at pH3. Growth increases linerly with an increase of soluble starch content up to 100g per liter medium, but 5 grams of ammonium sulfate per liter is the optimum nitrogen concentration for growth, if Pfeffer's medium is employed. Amylase activities of Rhizopus at different cultural periods showed that the maximum amylase production takes place after the cell population has reached its peak in the culture. Dextrinogenic amylase production has reached maximum at stationary phase, and maximum saccharogenic maylase production takes place in the pahse of negative gorwth acceleration.