• Journal of Microbiology and Biotechnology
• /
• v.19 no.7
• /
• pp.734-741
• /
• 2009

Recently, strains of methicillin-resistant Staphylococcus aureus (MRSA) with reduced susceptibility to vancomycin (VCM) have been clinically isolated. The antibacterial activity of a new drug, linezolid (LZD), in such a strain was evaluated by measuring bacterial metabolic activity. A total of 73 MRSA strains having various susceptibilities to VCM were subjected to a novel and highly sensitive chemiluminescence-based assay. LZD MIC in the tested strains, measured by the microbroth dilution method, was within the range 1-4 mg/l (mostly ${\leq}2$mg/l), except for one LZD-resistant strain (NRS127; MIC=7 mg/l), and showed no correlation with VCM resistance. The chemiluminescence assay demonstrated that bacterial metabolic activity was strongly suppressed with increasing LZD concentration. The chemiluminescence intensity curve had a low baseline activity without tailing in most strains. The present results suggest that LZD has strong antibacterial activity against MRSA strains, and would be effective for treatment of infections that are poorly responsive to VCM. The chemiluminescence assay facilitated sensitive and discriminative susceptibility testing within a relatively short time.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
• /
• v.15 no.1
• /
• pp.19-24
• /
• 2003

The objectives of this paper are to develop a new method of wettability mea-surement and to study the effect of surface roughness on the wettability in a $H_2O$/LiBr falling film absorber. Two absorber tubes with micro-scale roughness and a bare tube are tested in a falling film absorber installed in a test rig. Inlet solution temperature, concentration and mass flow rate are considered as key parameters. A new method is proposed to estimate the wettability of a tube by measuring a minimum mass flow rate to wet the tube completely. The wettability for the structured surfaces was higher than that for the bare tube. The wettability decreased linearly along the vertical location. The wettability increased with increasing the solution temperature and the solution mass flow rate. The experimental correlations of the wettability for the bare and the micro-hatched tubes were developed with error bands of$\pm20%\;and\;\pm10%$, respectively. This work can be used in the design of absorbers with micro-scale roughness.

• Journal of the Korean Society of Safety
• /
• v.21 no.6 s.78
• /
• pp.38-45
• /
• 2006

Polymeric floorings mainly consisted of PVC are easily decomposed by many kinds of hot environmental factors, then generate hazardous asphyxiate gases and/or toxic gases etc. Therefore the mechanism of decomposition and quantitative toxic indices of products are very important for preventing safety and health disasters, especially in case of confined area. So we have investigated decomposition kinetics, numbers of process involved, toxicity indices of product and so on, using DSC, TGA, FT-IR and Pyrolyzer-GC/MS. The thermal decomposition process of polymeric floorings can be mainly divided by dehydrochlorinated reaction and polyene decomposition step, and activation energies of those are approximately $53.93{\sim}62.42kcal/mol$. Especially lethal concentration($LC_{50}$), fractional effective dose (FED) are calculated by measuring the amount of decomposition product. The values on $LC_{50}$ of sample G are ranged $2,003{\sim}2,019(mg/m^{3})$ in case of sample K and H are $1,877,\;1,998(g/m^{3})$ respectively. Even if the results are estimated by calculation method without animal test and/or clinical demonstration, these values could be very useful data for occupational health, hygiene and safety control.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2001.11a
• /
• pp.42-71
• /
• 2001

Leakage detection system can possibly locate leak point without laboratory analysis. Several different types of sensors provide these benefits. But the use of these technologies is not widespread, mainly because of cost. Each of the leakage detection systems available has different advantages and disadvantages. The ideal system would be affordable, durable enough to last through the life of the landfill, automated, and applicable to all types of landfills. The laboratory tests were performed to investigate the relationship between electrical resistivity and the unsaturated subsurface condition and to evaluate the contamination due to leachate based on measuring electrical resistivity. The results of experiment show that the electrical resistivity of soil decreases as moisture density increases. The electrical resistivity of soil decreases as the concentration of leachate in pore fluid increases. These facts indicate that electrical resistivity method can be a promising tool in detecting of leachate. Also, the field model tests were conducted to verify that detection of leachate leak point on detection system using electrical characteristics is accurate. Field model test results of leakage detection system imply that the leakage detection system using electrical characteristics have the great potential of detecting exactly the leak point of leachate.

• Journal of Navigation and Port Research
• /
• v.34 no.1
• /
• pp.71-75
• /
• 2010

This study is experimented to observe an influence of experimental conditions on production characteristics of slurry ice by injection operating water to cooling plate. And at this experiment it used ethylene glycol-water solution and the concentration is 10 to 20wt%. The experimental apparatus was constructed of ethylene glycol-water solution and slurry ice storage tank, brine tank, pumps for ethylene glycol-water solution and brine circulating, a mass flow-meter, data logger for fluid temperature measuring and a vertical circular tube with single copper plate as test section. The experiments were carried out under various conditions, with mean velocity of fluid at the entry ranging from 1.0 to 2.0m/s and the cooling temperature of $-17^{\circ}C$ to $-10^{\circ}C$.

• Journal of Microbiology
• /
• v.38 no.4
• /
• pp.255-259
• /
• 2000

For rapid and sensitive measurement of antiviral activities, application of a recombinant virus containing firefly luciferase gene was attempted. Recombinant human cytomegalovirus (HCMV) containing luciferase gene driven by HCMV late gene pp28 promoter (HCMV/pp28-luc) was used to test the antiviral activities of three known compounds and the result was compared with results from the conventional plaque assay for measuring the production of infectious viruses. When human fibroblast cells were infected with HCMV/pp28-luc, luciferase activity was observed at 2 days after infection and reached maximum at 6 days after infection, whereas the production of infectious virus was maximal at 4 days after infection. The antiviral activities of ganciclovir, acyclovir, and papaverine were measured in HFF cells infected with HCMV/PP28-luc and the luciferase activity was compared with the infectious virus titers. Luciferase activity decreased as the concentration of ganciclovir or papaverine increased, while there was a slight decrease in luciferase activity with acyclovir. The level of the decrease in Luciferase activity was comparable to the level of decrease in the production of infectious virus. Therefore, the antiviral assay using recombinant virus HCMV/pp28-luc resulted in sensitivity similar to the conventional plaque assay with a significant reduction in assay time.

• Journal of the Korean Society of Visualization
• /
• v.21 no.1
• /
• pp.103-109
• /
• 2023

This research developed a measurement technique that can measure the oxygen temperature inside a high temperature furnace. Instead of measuring only changes in frequency components within a small range used in the existing variable laser absorption spectroscopy, laser spectroscopy technology was used to spread out wavelength of the light source passing through the gas Based on a total of 20,000 image data, research was conducted to predict the temperature of a high-temperature furnace using CNN with black and white images in the form of spectral bands by temperature of 25 to 800 degrees. The optimal model was found through Hyper parameter optimization, R2 score is 0.89, and the accuracy of the test data is 88.73%. Based on this research, it is expected that concentration measurement and air-fuel ratio control technology can be applied.

• Safety and Health at Work
• /
• v.13 no.4
• /
• pp.487-492
• /
• 2022

Objective: The exposure levels of disease-causing bacteria and germs were assessed on aircraft cleaning workers on multiple different aircrafts. Method: Five measuring points were selected depending on the aircraft types. Four aircraft cleaning agencies were selected for the test. Aircraft cleaning work was classified as intensive cleaning and general cleaning work. Ventilation in aircraft when sampling during the cleaning operation was categorized into forced ventilation and natural ventilation. The collection of airborne microorganisms was made through inertial impactors which were installed 1.5 meters above the bottom of the aircraft. The airborne bacteria and fungus growth badges were selected by Trytpic Soy Agar and Sabouraud Dextrose Agar. Results: The average concentrations of bacteria in the air were higher in the order of small, medium, and large airplanes. Rainy days had higher concentrations inside and outside the aircraft as compared to those in sunny days. Regarding ventilation, concentrations in natural ventilation were higher than concentrations in forced ventilation. According to the type of work, the concentrations in the intensive cleaning groups (cleaning one plane a day) were lower than those of the ordinary cleaning groups (cleaning several planes per day). Conclusion: The concentration levels of airborne bacteria and fungi in the aircraft surveyed were lower than the indoor environmental standards of Korea (800 cfu/m³ and 500 cfu/m³). The average concentrations of bacteria in the air and fungi in the air were highest in small aircraft owned by Company D.

• Culinary science and hospitality research
• /
• v.22 no.8
• /
• pp.149-156
• /
• 2016

The purpose of this study is to reduce the contaminants (total volatile organic compounds (TVOCs), fine particle, odor and total airborne bacteria) during cooking process in cooking chamber, and to decrease the health damage in indoor space that has bad work environment. In order to solve the shortcomings of existing air purifiers and remove all kinds of pollutants effectively, this study focused on the development of indoor air purifiers which are made of bar type. Bio-ceramics filter which combines activated carbon and loess. The air cleaners developed with 4 measuring items including TVOCs, particulate matter, complex odor and total airborne bacteria were measured comparing their pre-service test to their post-service test after a period of time. The measured results showed higher removal efficiency of 91.02% as the concentration of TVOCs was reduced from $2,500{\mu}g/m^3$ to $223{\mu}g/m^3$. Second, the particulate matter removal ratio was 97.51% efficient with average concentration of $26.68{\mu}g/m^3$. Third, the odor showed 95.20% reduction as air dilution ratio averaged out at 144. Last, total airborne bacteria was eliminated by over 94% showing the changeable concentration from $787{\sim}814CFU/m^3$ to $47{\sim}40CFU/m^3$. In addition, the removal rate of harmful pollutants is excellent, and it is expected that the environment of the existing poor cooking room will be greatly improved by using the developed air purifier in combination with the ventilation device and the stove hood.

• Journal of Korean Society for Atmospheric Environment
• /
• v.33 no.6
• /
• pp.616-625
• /
• 2017

Measurements using five real-time particle samplers were compared to measurements using three NRM (National Reference Method system) filter-based samplers(Gravimetric method) at Incheon, Korea, between May and August, 2014. The purpose of this study was to suggest the quality assurance/quality control (QA/QC) method of each instrument for use in a real-time continuous particle sampler to measure the mass of airborne particles with an aerodynamic diameter less than $2.5{\mu}m$ ($PM_{2.5}$). Five real-time particle samplers of BAM1020, FH62C_14, TEOM, PM-711 and SPM-613 were evaluated by comparing its measured 23 hr average $PM_{2.5}$ concentrations with those measured with NRM filter-based samplers simultaneously. The parameters(e.g. Inlet heating condition, Slope factor, Film response, Intercept, Background, Span value) of the real-time samplers were optimized respectively by conducting test performance evaluation during 7 days in field sampling. For example, inlet heating temperature of TEOM sampler controls $35{\sim}40^{\circ}C$ to minimize the fluctuation of the real-time measurement data and background value of BAM1020 is the key factor affecting the accuracy of $PM_{2.5}$ mass concentration. We classified the $PM_{2.5}$ concentration according to relative humidity (80%) to identify water absorbed in aerosols by measuring the ${\beta}$-ray samplers(BAM1020, FH62C_14) and TEOM. ${\beta}$-ray samplers were not strongly affected by relative humidity that the difference of the average $PM_{2.5}$ concentration was about 5%. On the other hand, The TEOM sampler overestimated $PM_{2.5}$ mass concentration about 15% at low relative humidity (<80%).