• Applied Biological Chemistry
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• 제41권1호
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• pp.110-117
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• 1998

Ghana산 cacao bean으로부터 acetone으로 추출하고 Sephadex LH-20, MCI-gel CHP-20, Bondapak $C_{18}$ 및 Fuji gel ODS $G_3$, chromatography 등을 이용하여 7종의 polyphenol 화합물(compound 1 - compound 7)을 분리 정제하였고, 이들 화합물의 화학구조를 $^1H-NMR$, $^{13}C-NMR$, IR 및 MS를 사용하여 검색하였고 아울러 angiotensin converting enzyme(ACE) 저해효과를 조사 검토하였다. 분리정제한 7종의 polyphenol 화합물의 구조를 분석한 결과, compound 1: [(+)-catechin], compound 2: [(-)-epicatechin], compound 3: [procyanidin B-1, (-)-epicatechin-$(4{\beta}{\rightarrow}8)$-(+)-catechin], compound 4 : [procyanidin B-2, (-)-epicatechin-$(4{\beta}{\rightarrow}8)$-(-)-epicatechin], compound 5: [procyanidin B-7, (-)-epicatechin-$(4{\beta}{\rightarrow}6)$-(+)-catechin], compound 6 : (procyanidin B-2,3,3'-O-digallate) 및 compound 7: [cinnamtannin A-2, (-)-epicatechin-$(4{\beta}{\rightarrow}8)$-(-)-epicatechin-$(4{\beta}{\rightarrow}8)$-(-)-epicatechin-$(4{\beta}{\rightarrow}8)$-(-)-epicatechin]임을 동정하였다. ACE의 저해효과는 procyanidin B-2,3,3'-O-digallate (compound 6)가 $100\;{\um}M$에서 94.6%로 매우 우수하였으며, (+)-catechin (compound 1), (-)-epicate -chin (compound 2), procyanidin B류 (compound 3, 4, 5) 및 cinnamtannin A-2 (compound 7)도 각각 67.9%, 61.9%, 88.6%, 82.5%, 72.2% 및 82.3%의 비교적 우수한 저해효과가 있었다. 아울러 결합방식에서는 $4{\beta}{\rightarrow}6$보다 $4{\beta}{\rightarrow}8$의 결합방식이, 그리고 procyanidin 류에서는 gallate를 갖는 물질이 이를 함유하지 많은 물질보다 더욱 높은 ACE의 저해효과를 나타내었으며, 또한 hydroxyl 기가 많을수록 효소 저해효과도 증가하는 것으로 나타났다. 이와 같은 결과는 chocolate의 주원료인 cacao bean의 polyphenol 성분 또한 녹차 등에서 볼 수 있는 생리활성효과에 손색없는 것으로 판단되고, 이러한 기능성에 기초하여 chocolate, 음료 등의 식품이나 의약품의 기능성 소재로서의 산업적 응용 가능성이 높은 것으로 사료된다.

• 대한기계학회논문집B
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• 제27권1호
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• pp.54-60
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• 2003

Compound choking frequently occurs at a minimum area of the flow passage, where two or more streams which have different stagnation properties are merged. This phenomenon is especially important in that the flow choking may not be given by Mach number, M=1 at the nozzle throat. In order to obtain a detailed understanding of the flow characteristics involved in the compound flow choking, the two-dimensional, compressible, Wavier-Stokes equations are solved using a fully implicit finite volume method and the predicted solutions are compared with the results of the one-dimensional theoretical analysis. Stagnation pressure and temperature of each stream are changed to investigate the effects on the compound choking. The results show that stagnation pressures of each stream affect Mach number and static pressure distributions downstream of the exit of the convergent nozzle. However, the flow characteristics of the compound choking are not significantly dependent on the total temperature ratio.

• Journal of Microbiology and Biotechnology
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• 제27권6호
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• pp.1065-1070
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• 2017

This study aimed to examine the anti-candidal efficacy of a novel ketone derivative isolated from Diaporthe sp. ED2, an endophytic fungus residing in medicinal herb Orthosiphon stamieus Benth. The ethyl acetate extract of the fungal culture was separated by open column and reverse phase high-performance liquid chromatography (HPLC). The eluent at retention time 5.64 min in the HPLC system was the only compound that exhibited anti-candidal activity on Kirby-Bauer assay. The structure of the compound was also elucidated by nuclear magnetic resonance and spectroscopy techniques. The purified anti-candidal compound was obtained as a colorless solid and characterized as 3-hydroxy-5-methoxyhex-5-ene-2,4-dione. On broth microdilution assay, the compound also exhibited fungicidal activity on a clinical strain of Candida albicans at a minimal inhibitory concentration of $3.1{\mu}g/ml$. The killing kinetic analysis also revealed that the compound was fungicidal against C. albicans in a concentration- and time-dependent manner. The compound was heat-stable up to $70^{\circ}C$, but its anti-candidal activity was affected at pH 2.

• 한국의류산업학회지
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• 제11권1호
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• pp.6-13
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• 2009

The purpose of this study is to analyze the sensibility of the compound fashion image of contemporary fashion. The specific objectives were to analyze 1)the structure of meaning about fashion sensibility on 2)the difference of fashion sensibility by categories on 3)the difference of fashion sensibility by demographical aspects on 4)the consumer sensibility on the compound fashion image. For collecting data 55 stimulus and questionnaire which consisted of bi-polar 21 pairs adjective scale were contributed 675. The results were summarized as following : 1. Factors of fashion sensibility consist of estheticism, activeness, personality and feminity. And the meaning of fashion sensibility is young and personalty. 2. The man/woman and the past/present are highly estimated personality, the main/subcultural is activeness and the local/global is feminity of fashion sensibilities. 3. gender and age have high difference of the compound fashion image so they are important variance for fashion sensibilities. 4. Preference, purchase and quality are highly influenced by estheticism, pleasure is influenced by personality. The results of this study will be utilized in the clothing design for accepting customers' specific fashion sensibility about compound fashion image.

• 생약학회지
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• 제31권4호
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• pp.449-454
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• 2000

In search for plant-derived cytotoxic compounds, it was found that the $CHCl_3$ and EtOAC extracts obtained from the flowers of Paulownia coreana Uyeki (Scrophulariaceae) exhibited significant cytotoxic activity against human tumor cell lines, A549, SK-OV-3, SK-MEL-2, XF498, and HCT15. Activity-guided fractionation on the basis of the inhibitory activity against the growth of human tumor cell lines, in vitro, and repeated column chromatography afforded several cytotoxic compounds from P. coreana. The structures and stereochemistry of these compounds were established, on the basis of analysis of spectra including IR, UV, EI-MS, $^{1}H-NMR,\;^{13}C-NMR$ and some chemical transformations, as Compound PCCl $(2-hydroxy-4(15),11(13)-eudesmadien-8{\beta},12-olide)$, Compound $PCC2(2,3-dihydro-4-hydroxy-1(15),11(13)-xanthadien-8{\beta},12-olide)$, Compound PCE1 (chrysophanol), Compound PCE2 (emodin), Compound PCE3 (physcion). Cytotoxic activity of compounds obtained from P. coreana. on five tumor cells lines was evaluated by procedure of SRB methods.

• Archives of Pharmacal Research
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• 제8권3호
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• pp.149-157
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• 1985

The HCL hydrolyzate of the non-histone protein fractionated from the rat liver nuclei which have been incubated inthe presence of S-adenosyl-L-[methyl-$^{14}C$ ]-methionine shows at least four unidentified radioactive peaks on a basic amino acid analysis chromatogram. One of these unknown compounds (designated as compound 3) is also formed by the rat liver homogenated with the exogenous addition of an appropriate protein substrate. Since boiled rat liver homogenate or fresh homogenate in the absence of an exogenous protein substrate failed to form compound 3, its formation can be considered to be enzyme-catalyzed. The enzyme which yields compound 3 shows a preference of protein substrate in the order of reductively methylated hemoglobin > native > histone type II-A. The rat enzyme is nuclear in location associated with chromatin, and exhibits the highest activity in the liver among various rat organs. A compound 3-forming enzyme is also present in Neurospora crassa, since endogenous formation of the compound 3 can be demonstrated with the crude extract of this mold. The chemical identity of compound 3 is not yet known. However, it resisted to the following treatments; 6 N HCL and 0.1 N Na NaOH hydrolysis at $110^{\circ}C$, OR L-amino acid oxidase.

• 대한한의학방제학회지
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• 제29권4호
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• pp.267-275
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• 2021

Objectives : Previously, we reported that compound K isolated from fermented ginseng by Aspillus oryzae has a wide biochemical and pharmacological effect, including anti-cancer activity in prostate cancer PC-3 cells. Despite these findings, its signaling pathway and gene expression pattern are not clearly understood. Methods : To confirm the gene expression study of treated with compound K in PC-3 cells, a cDNA microarray chip composed of 44K human cDNA probes was used. MTT assay, western blot analysis, propidium iodide staining, and annexin V/propidium iodide staining were analyzed. Results : We confirmed the differences of gene expression profiles. Then, we analyzed with the cell cycle arrest, cell death and cell proliferation related genes using DAVID database. Conclusions : Our finding should be useful for understanding genome-wide expression patterns of compound K-mediated cell cycle arrest toward induction of cell death and be helpful for finding future cancer therapeutic targets for prostate cancer cells.

• Earthquakes and Structures
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• 제23권4호
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• pp.363-371
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• 2022

Traditional metal dampers have disadvantages such as a higher yield point and inadequate adjustability. The experimental results show that the low yield point steel has superior energy dissipation hysteretic capacity and can be applied to seismic structures. To overcome these deficiencies, a novel compound metal damper comprising both low yield point steel plates and common steel plates is presented. The optimization objectives, including "maximum rigidity" and "full stress state", are proposed to obtain the optimal edge shape of a compound metal damper. The numerical results show that the optimized composite metal damper has the advantages such as full hysteresis curve, uniform stress distribution, more sufficient energy consumption, and it can adjust the yield strength of the damper according to the engineering requirements. In view of the mechanical characteristics of the compound metal damper, the equivalent model of eccentric cross bracing is established, and the approximate analytical solution of the yield strength and the yield displacement is proposed. A nonlinear simulation analysis is carried out for the overall aseismic capacity of three-layer-frame structures with a compound metal damper. It is verified that a compound metal damper has better energy dissipation capacity and superior seismic performance, especially for a damper with double-objective optimized shape.

• 대한기계학회논문집A
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• 제37권1호
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• pp.55-60
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• 2013

컴파운더 보우는 리커브 보우와 비교하여 케이블 및 캠 풀리로 구성되는 지레시스템을 사용하여, 궁사가 보우를 최대 위치로 당기기 위해 필요로 하는 힘을 줄이는 동시에 더 많은 에너지를 축적할 수 있도록 해준다. 지금까지 컴파운더 보우의 효율 및 성능을 향상시키기 위해 경험적인 방법에 기초하여 많은 특허가 제안되었지만, 해석적인 방법에 대한 연구는 별로 없었다. 그러므로 본 연구에서는 컴파운더 보우 지레 시스템의 기구학적인 해석을 위한 방법을 제안하여 지레시스템의 캠 형상 및 보우 날개의 재질변화에 따른 효과를 설계자가 쉽게 예측할 수 있도록 하고자 한다.

• Preventive Nutrition and Food Science
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• 제14권3호
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• pp.173-178
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• 2009

This study was conducted to determine if supplementation with a compound composed of caffeine (50 mg), capsaicin (75 mg), sesamine (30 mg), L-carnitine (300 mg), banaba (50 mg) and lotus (10 mg) enhanced human autonomic nervous activities (ANS) associated with thermogenic sympathetic activity and fat utilization. Ten healthy college males (21.2$\pm$1.0 yr) volunteered for this experiment. Autonomic nervous activities associated with energy metabolism were examined at 30 min intervals for a total of 120-min while at rest and every 5-min during exercise at 50% of the ventilation threshold before and after intake of the compound or placebo with 100 ml of water for 10 days. In addition, heart rate variability power spectral analysis was used to assess human autonomic nervous activities. The results indicated that there were no significant differences in heart rate during rest and exercise among trials. Furthermore, the autonomic nervous activity tended to increase after 10-days of consumption of the test compounds during the experimental period, but the differences did not reach statistical significance. However, before and after the compound test trial there was a significantly higher respiratory gas exchange ratio (rest 0: 0.83$\pm$0.01 vs. rest 3: 0.89$\pm$0.02, p<0.05), carbohydrate oxidation (CHO) rate (rest 0: 44.57$\pm$5.83 vs. rest 2: 63.86$\pm$5.91%, p<0.05) and a lower fat oxidation rate (rest 0: 55.43$\pm$5.83 vs. rest 2: 36.14$\pm$5.91%, p<0.05. In conclusion, the results of the present study suggested that the compound composed of caffeine, capsaicin, sesamine, L-carnitine, banaba and lotus components that was evaluated in this study did not induce a significant increase in human autonomic nervous activities or lipolysis, even though the individual components have been reported to induce increased fat oxidation.