• Journal of Ginseng Research
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• 제17권3호
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• pp.219-223
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• 1993

Assessment of root weight grade system for the numeral definition in Korea ginseng was tried by investigation of 13 boxes of the 15-root-grade of 4-year-old white ginseng as a model. In this case satisfaction to theoretical value was 58% in root weight and 50% in root number. The coefficient of variation (CV) was high (35%) for individual root weight in package. The CV of tap root weight 1 among package is low (7.1%) even though there was no significant correlation between tap root weight : and lateral root weight among roots. The mean percentage of tap root to root weight in each package ranged 61 to 74% with CV range of 16.5 to 33.2%. The CV was not much different between 1st and 2nd lateral root weight and the means was above 60%. Strict practice of numeral definition of root weight grade system will be prerequisite for chemical standardization of Korea ginseng.

• 한국작물학회지
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• 제43권4호
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• pp.273-278
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• 1998

Carbohydrate metabolism and partitioning are dependent on relationships between sources and sinks which can be affected by rates of photosynthesis and respiration. Fructan, the major form of stored carbohydrate in tall fescue (festuca arundineacea Schreb.), changes in concentration during growth and in response to the environment. Objectives of this study were i) to examine the content and the composition of carbohydrates in five tissues (mature leaf blade, immature leaf blade, leaf elongation zone, terminal meristem, and root tips) of two tall fescue genotypes, one with high yield per tiller (HYT) and one with low yield per tiller (LYT), and ii) to compare the reserved and utilized carbohydrates among above five different tissues, particularly between the leaf elongation zone and root tips. The established vegetative tillers of the HYT and LYT genotypes were grown in a controlled-environment growth chamber. Water-soluble carbohydrate (WSC) in the leaf elongation zone was about 22% of dry weight in the HYT and about 19% in the LYT genotype. The root tip also had high WSC, about 12% of dry weight in the HYT and 6% in the LYT genotype. Hexoses and sucrose were the major components of total WSC in all tissues except the leaf elongation zone. The growing tissues (sinks), i.e., the leaf elongation zone and root tip, had a high proportion of low degree of polymerization fructan, i.e., 3 to 8 hexose units.

• Journal of Ginseng Research
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• 제11권1호
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• pp.10-16
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• 1987

인삼제품의 품질관리와 효능연구의 시료사용에 참고자료로 활용하기 위하여 인삼근의 부위별, 연근별 사포닌 함량 및 분포를 조사하였다. 한국인삼 6년근의 부위별 조성은 뇌두(rhizome) 4.1%, 동체(main root) 47.7%, 지근(lateral)의 비는 대략 1:1이었으나 지근부는 2:1로 피층이 많았다. 사포닌분포는 동체와 세근부가 29% 정도로 거의 같고, 지근부 34.6%로 가장 많았고, 뇌두는 7% 수준으로 가장 적었다. 그러나 단위 중량당 함량에서는 세근>퇴두>지근>동체의 순으로 뇌두가 높은 반면 동체는 가장 낮았다. 연근별 사포닌 변화는 2년근 4.89%, 6년근 7.98%로서 본포이식후 4년간 약 3% 증가에 그쳤으나 뿌리의 비대성장을 고려할 때 계속적으로 합성축적 되었으며 연간 증가율은 5년근이 43.8%로 가장 왕성한 성장기임을 나타내고 4년근 19.8%, 6년근 21.1%로 비슷했다. 따라서 5년에 뿌리의 생장과 사포닌 합성축적이 최고 수준에 도달했다가 6년째 부터 쇠퇴기로 됨을 알았다. 개별 사포닌의 함량변화는 전반적으로 증가하는 경향이나 PT계 사포닌보다는 PD계 사포닌의 증가가 보다 큰 경향이었다.

• 원예과학기술지
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• 제18권6호
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• pp.819-822
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• 2000

장미의 양액재배 및 상자재배시 필수적인 우량 삽목묘의 육성을 위하여 장미의 삽목발근에 미치는 삽목용토의 영향을 실험한 결과, 품종에 따라 다소간의 차이는 있었지만 피트모스와 대립 버미큘라이트 1:2의 혼합용토에서 발근이 가장 양호한 경향을 보여 대부분의 품종에서 100%의 발근율을 보였으며 'Little Mable'과 'Suplesse'도 95% 이상의 발근율을 보였다. 또한 뿌리의 생육상태를 조사한 결과, 뿌리의 수, 무게 및 직경 등도 피트모스와 대립 버미큘라이트 1:2혼합토에서 생육이 가장 양호한 경향을 보였으나 뿌리의 길이는 피트모스와 대립 펄라이트 1:2 혼합토에서 가장 긴 경향을 보였다.

• The Korean Journal of Ecology
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• 제26권5호
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• pp.263-266
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• 2003

This research investigated the process of removing cadmium and tested the detoxification mechanism of the cadmium-binding peptide (Cd-BP) from Rumex crispus. Phytochelatin-like cadmium-binding peptide (PC-Cd-BP) of Rumex crispus was purified and identified. Rumex crispus was exposed to 4.3 mg Cd/L for seven days. Heat-treated supernatant fraction taken by root tissues showed traces of PC-Cd-BP An analysis of the material through Gel-filteration chromatography on the Sephadex G-75 column showed two symmetrical Cd-BP peaks. The major peak with the smaller molecular weight was further purified by $C_{18}$ reverse-phase HPLC to produce apparent homogeneity. The amino acid composition of Cd-BP from Rumex crispus included cysteine (22.6%), glutamate and glutamate acid (20%), and glycine (12%). It was similar the amino acid composition of most PC. The molecular weight of the purified peptide was determined at 568-706 Da by MALDI-TOF MS. Therefore, the Cd-BP of Rumex crispus was PC-Cd-BP consisting of isopeptides.

• Plant Resources
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• 제6권3호
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• pp.183-187
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• 2003

Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica

• Journal of Nutrition and Health
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• 제16권2호
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• pp.115-124
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• 1983

The biochemical nature of the protein constituents of six year old fresh Panax ginseng root was studied. Total protein constituents were extracted with phosphate buffer of pH 7.4, ionic strength of 0.1 and fractionated by ultrafiltration using four different membranes which cut down the materials of molecular weight of 500, 1,000, 5,000 and 10,000, respectively. Each fraction was subjected to ion exchange chromatography using DEAE - cellulose to isolate component proteins. The protein fraction larger than molecular weight of 10,000 was refractionated by the method of ammonium sulfate precipitation. The electrophoresis of the refractionated protein constituents was performed. The amino acid composition of the protein constituents was determined by gas- liquid chromatography. From the results, it could be summarized that eleven different protein constituents smaller than molecular weight of 10,000 were isolated from the fresh Panax ginseng root. At least eleven different protein constituents larger than molecular weight of 10,000 were identified from the electrophoretic patterns. These protein costituents seem to be compounded of all or some of five different subunits.

• 한국식품영양학회지
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• 제7권4호
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• pp.267-273
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• 1994

To provide the effective application scheme and basic information of buckwheat (Fagopyrum esculentum Moench), buckwheat was germinated at 10$^{\circ}C$ for 7 days and 100 kernels weight, germination rate, root length, chemical composition and mineral contents were examined at 24 hour Intervals. During the germination period, the 100 kernels weight increased approximately 0.3g per day. The germination rate increased sharply after 2 days and the root length increased greatly after 4 days. The crude protein contents increased with germination time, whereas the carbohydrate contents decreased. The crude ash and fat contents did not differ significantly during the germination period. The Ca contents Increased for the 4th day of gemination, but gradually decreased afterwords. The Na contents increased in the initial stage of germination, but then gradually decreased. However, there were no significant change In the Mg, K. Fe. Mn and Zn contents.

• 대한한방소아과학회지
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• 제25권3호
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• pp.77-84
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• 2011

Objectives: This study was performed to investigate the effect of hot spring therapy on children weight, body composition and heart rate variability. Methods: The study was carried out from 30 children who visited the spa from August 1, 2011 to August 31, 2011. We measured the change of weight, body composition, and heart rate variability after hot spring therapy for an hour per day. Results: The subject was consisted of 13 boys and 17 girls. This study revealed that their weight got lost from a hot spring therapy, which was statistically significant. Their body mass index(BMI) was decreased in from a hot spring therapy, which was not statistically significant. Total body water, muscle, protein, and mineral were decreased from a hot spring therapy, which was statistically significant. We measured HRV(heart rate variability) before and after the hot spring therapy. The mean HRT was increased, which was statistically insignificant. SDNN(standard deviation of the NN interval) and RMSSD(square root of the mean of the sum of the square of differences) were not different before and after the hot spring therapy. ln TP was decreased insignificantly. ln VLF was not affected before and after hot spring therapy. ln LF and ln HF was decreased, LF norm was increased, and HF norm was decreased, which were not statistically significant. Also, LF/HF ratio was increased insignificantly. Conclusions: From these results, we concluded that the weight, total body water, muscle, protein, and mineral got lost from the hot spring therapy. However, the HRV was not affected from the hot spring therapy.

• Journal of Crop Science and Biotechnology
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• 제11권1호
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• pp.57-62
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• 2008

Isoflavone content in various parts of six soybean cultivars and soybean sprout during germination was analyzed by high performance liquid chromatography. The parts analyzed were seed coat, cotyledon, and axis for seeds and whole sprout, root, hypocotyl, and cotyledon for sprout. Two cultivars, Aga3 which is known to have the smallest seed size and the highest isoflavone content among the Korean soybean cultivars and Pungsannamulkong which is the most widely being used as soy-sprout, were selected for sampling from 1 to 10 days after germination. At the same weight, the order of isoflavone content increased from seed coat to cotyledon to axis. The highest total isoflavone(isoflavone$\times$dry weight) content was observed in the cotyledon and the lowest in the seed coat. The cotyledon of the Aga3 variety had the highest total isoflavone content and the lowest was measured in the Pungsannamulkong variety. The highest total isoflavone content, $10,788{\mu}g/g$, was observed in whole sprouts(cotyledon+hypocotyl+root) on day 7 for Aga3. After day 7, there was a decreasing trend in isoflavone content as the germination period increased. Total isoflavone content in the cotyledon of Aga3 significantly increased after seed germination, whereas the isoflavone content in the cotyledon of Pungsannamulkong decreased. However, total isoflavone content in the root of both varieties increased while isoflavone content in the hypocotyls decreased after seed germination.