• 한국작물학회지
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• 제49권3호
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• pp.160-166
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• 2004

벼 M/G RIL 162 계통에 대한 초기급속등숙율에 대한 이화학적특성 및 색차색도특성 변이에 대한 주성분분석 및 유전력분석의 결과를 요약하면 다음과 같다. 1. 초기급속등숙율이 41％ 이하인 계통군은 164계통중 74계통이었으며, 41∼60％의 계통군에는 32계통이 있었다. 또한, 초기급속등숙율이 빠른 계통군에 속하는 61∼80％사이에는 24계통이 있었으며, 초기급속등숙율이 매우 빠른 계통군으로 분류되는 81％ 이상인 계통은 32계통이 있었다. 2. 벼 M/G RIL 164계통에 대한 품종군별 이화학적특성을 보면, 초기급속등숙을이 40％ 이하인 계통군이 단백질 함량이 9.1％로 가장 높았으며, 지방산함량은 초기급속등숙율이 61∼80％의 계통군에서 26.5KOHmg/100g으로 가장 높게 나타났다. 또한, 곡립형태에 있어서 현미길이는 41∼60％사이의 초기급속등숙율을 보인 계통군이 5.40mm로 가장 짧았으며, 81％ 이상인 계통군이 1.80mm로 현미두께가 가장 두꺼웠으며, 현미의 형태를 나타내는 현미 길이/넓이 비에 있어서는 41∼60％사이의 계통군이 2.18로 나타났다. 3. 호화온도와 깊은 관련이 있는 알카리붕괴도에 있어서는 초기급속등숙율이 61∼80％사이가 3.40으로 가장 붕괴가 되지않는 계통군으로 나봤으며, 81％이상인 계통군은 4.31로서 붕괴가 가장 잘 되는 것으로 나타나 81％의 계통군이 호화온도가 낮은 것으로 나타났다. 4. 화학적특성, 색차색도특성 및 알카리붕괴도를 이용한 벼M/G RIL 164 계통에 대한 주성분분석의 결과 제 1주성분은 약 3.6개의 형질을 포함하고 있었으며, 제 2주성분은 약 2.1개의 형질을 포함하고 있는 것으로 밝혀졌다. 또한, 제 1주성분은 전체 변동에 대한 기여율은 약 36％이었으며, 제 4주성분까지의 전체 변동에 대한 누적 기여율은 86.4％로서 설명이 가능함을 알 수 있었다. 5. 벼 M/G RIL 164 계통의 품질평가치, 단백질함량, 아밀로오스함량, 지방산함량, 명도, a-value, b-value, 채도, 색상 및 알카리붕괴도 등의 형질에 대한 유전력 검정의 결과, 품질평가치의 유전력은 87.89％로 가장 높은 유전력을 나타내었으며, 단백질함량 ＞ 알카리붕괴도 ＞ 지방산함량 ＞ 아밀로오스함량 ＞ 색상 ＞ 명도 ＞ a-value ＞ b-value의 순으로 나타났으며, 채도의 유전력은 30.62％로 가장 낮음을 알 수 있었다.

• IMMUNE NETWORK
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• 제4권1호
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• pp.23-30
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• 2004

Background: A human orthologue of mouse S100A6-binding protein (CacyBP), Siah-1-interacting protein (SIP) had been shown to be a component of novel ubiquitinylation pathway regulating $\beta$-catenin degradation. The role of the protein seems to be important in cell proliferation and cancer evolution but the expression pattern of SIP in actively dividing cancer tissues has not been known. For the elucidation of the role of SIP protein in carcinogenesis, it is essential to produce monoclonal antibodies specific to the protein. Methods: cDNA sequence coding for ORF region of human SIP gene was amplified and cloned into an expression vector to produce His-tag fusion protein. Recombinant SIP protein and monoclonal antibody to the protein were produced. The N-terminal specificity of anti-SIP monoclonal antibody was conformed by immunoblot analysis and enzyme linked immunosorbent assay (ELISA). To study the relation between SIP and colon carcinogenesis, the presence of SIP protein in colon carcinoma tissues was visualized by immunostaining using the monoclonal antibody produced in this study. Results: His-tag-SIP (NSIP) recombinant protein was produced and purified. A monoclonal antibody (Korea patent pending; #2003-45296) to the protein was produced and employed to analyze the expression pattern of SIP in colon carcinoma tissues. Conclusion: The data suggested that anti-SIP monoclonal antibody produced here was valuable for the diagnosis of colon carcinoma and elucidation of the mechanism of colon carcinogenesis.

• Asian-Australasian Journal of Animal Sciences
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• 제16권10호
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• pp.1475-1481
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• 2003

The aim of this paper was to use the Cornell Net Carbohydrate and Protein System (CNCPS), that reports diet energy and protein value and animal requirements, as net energy for lactation ($NE_1$) and metabolizable protein (MP) respectively, to evaluate some rations for lactating Italian Mediterranean buffaloes. The investigation was carried out on six farms in the province of Caserta (southern Italy), where the milk production was controlled four times monthly on 10 animals (changing every time) chosen at different lactation days (5 categories): <2 months (A), 2-4 months (B), 4-6 months (C), 6-8 months (D), >8 months (E). Milk fat and protein were determined. Diet $NE_1$ and MP were estimated with the CPM-Dairy program (1998) using diet component chemical characteristics; then energy and protein intakes were estimated. $NE_1$ and MP requirements were estimated with two methods: 1) using CPM-Dairy that considers produced milk, fat and protein content, lactation phase and body condition score as main factors; 2) by applying the theory that to produce 1 kg of energy corrected milk, the buffalo needs 3.56 MJ of $NE_1$ and the efficiency to convert the absorbed aminoacids into milk protein is lower than cow (CNCPS). As regards energy, with method 1 the requirements were satisfactory starting from category A (4 out of 6 farms) and category B (5/6 farms); however, a surplus resulted for category E (5/6 farms). With method 2 a deficit in category A (5/6 farms) and B (3/6 farms) was observed, while the energy requirements were satisfied for all categories except E, where on only one buffalo farm had a surplus of energy intake. As regards protein, with method 1 the requirements were substantially satisfied for all the categories except E (3/6 farms); with method 2 the MP trend was much less favourable than with method 1. Indeed, a protein deficit was observed for all animals in categories A and B (5/6 farms). Moreover, on one farm the protein intake never satisfied animal requirements. In our experimental conditions, the use of the CNCPS to characterise diets for lactating buffalo and to calculate their requirements led to satisfactory results. By contrast, we cannot say the same for method 2, which applies a lower use efficiency of NE and MP for lactation in buffalo compared to cow.

• 한국수산과학회지
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• 제16권3호
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• pp.190-196
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• 1983

1년생과 3년생의 이스라엘잉어를 시료로 택하여 단백질의 조성과 육의 구성 및 유리아미노산조성의 차이를 비교 검토하였으며, 분획한 근형질단백질과 근원섬유단백질 및 세포내잔사단백질은 SDS-polya-crylamide gel 전기영동분석에 의하여 그 구성 subunit의 차이에 관하여도 검토하였다. 근육단백질의 조성을 분석한 결과, 1년생은 5년생에 비하여 근원섬유단백질의 양이 $6\%$정도 많았고, 근형질단백질과 세포내잔사단백질 및 기질단백질은 3년생이 1년생보다 조금 많았다. 그리고 전기영동상을 해석한 결과, 1년생과 3년생의 근형질단백질은 각각 11개와 10개의 subunit로 구성되어 있었고, 1년생의 분자량 210,000에 해당하는 subunit가 3년생에서는 검출되지 않는 점을 보였다. 근원섬유단백질에 있어서는 1년생과 3년생이 모두 $7.5\%$와 $10\%$ gel 전기영동가교를 사용했을 때의 분리된 subunit를 합하면 23개의 subunit로 구성되고 있었으며, 연령별에 따른 subunit 조성차이는 거의 없었다. 세포내잔사단백질에 있어서도 1년생과 3년생간에는 차이가 없음을 알았다. 한편, 육의 단백질구성아미노산을 분석한 결과, 두 연령별 시료간에 구성아미노산의 양적인 비율은 비슷하였다. 그리고 두 연령별의 시료가 모두 글루탐산, 아스팔트산 및 라이신을 어느 정도 많이 함유하고 있었다. 유리아미노산의 조성에 있어서도 3년생은 아스팔트산, 이소류신 및 페닐알라닌 등이 조금 많았고, 라이신, 히스티닌, 알기닌, 글루탐산등의 양은 조금 적었으며, 유리아미노산의 총양은 1년생이 3년생보다 많았다.

• BMB Reports
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• 제42권2호
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• pp.96-100
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• 2009

Aromatic (ar)-turmerone from turmeric oil displays anti-tumorigenesis activity that includes inhibited cell proliferation. This study investigated ar-turmerone-mediated apoptotic protein activation in human lymphoma U937 cells. Ar-turmerone treatment inhibited U937 cell viability in a concentration-dependent fashion, with inhibition exceeding 84%. Moreover, the treatment produced nucleosomal DNA fragmentation and the percentage of sub-diploid cells increased in a concentration-dependent manner; both are hallmarks of apoptosis. The apoptotic effect of ar-turmerone was associated with the induction of Bax and p53 proteins, rather than Bcl-2 and p21. Activation of mitochondrial cytochrome c and caspase-3 demonstrated that the activation of caspases accompanied the apoptotic effect of ar-turmerone, which mediated cell death. These results suggest that the apoptotic effect of ar-turmerone on U937 cells may involve caspase-3 activation through the induction of Bax and p53, rather than Bcl-2 and p21.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.186-189
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• 2003

Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a potent immunogen as well as major virulence factor. In order to express the recombinant urease in tobacco plants, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a plant expression vector. The recombinant vector was transformed to tobacco plants. The integration of the recombinant plasmids into tobacco chromosomal genome was verified by genomic PCR. Expression to mRNA was confirmed by Northern blot analysis, and expression to recombinant urease protein was observed by Western blot analysis. These results showed that the recombinant urease can be produced in tobacco plants and will be tested for immune response to use as an edible vaccine.

• 한국잠사학회:학술대회논문집
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• 한국잠사학회 2003년도 International Symposium of Silkworm/Insect Biotechnology and Annual Meeting of Korea Society of Sericultural Science
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• pp.153-154
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• 2003

Trichoptera, or caddisflies, comprise one of the major aquatic insect orders. Like Lepidoptera, caddisflies are capable of spinning silk from specially modified salivary glands, and the diversity of ways this silk is used probably accounts for the success of the order as a whole. These utilize silk to construct both larval and pupal shelters, often incorporating materials from the environmental among the silk thread. In this study, we try to find and characterize novel type genes that should be translated to major component protein of aquatic silk. (omitted)

• 한국수정란이식학회지
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• 제14권1호
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• pp.47-57
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• 1999

Among proteins separated from methanol extract of follicular fluid with superose column, the components inducing sperm swim-up separation through sucrose layer were analysed with superose column in Smart system and SDS-PAGE. And the results obtained were as follows; The fractions of retention volume (RV) 0.83ml and RV 1.36ml separated with superose column should stimulate sperm migration and movement. However, RV 0.83 fraction was consisted of complex materials containing RV 1.36 component. RV 1.36 fraction contained a BSA analogue of 67 kilodaltons (Kd) and showed identical peak pattern with BSA fraction V. In conclusion, the protein of 67 Kd in follicular fluid should stimulate sperm migration and movement.

• 한국자기공명학회논문지
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• 제20권1호
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• pp.17-21
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• 2016

Periostin, a component of extracellular matrix (ECM) protein, is produced and secreted by the fibroblasts that are involved in chronic allergic inflammation diseases and various types of human cancers. Periostin protein is composed of multiple domains including four FAS1 domains which play important roles in cell adhesion and tumor metastasis by interacting with integrins. In spite of their important biological role, the structural information of periosin FAS1 domains was not revealed yet. Recently we systemically prepared various constructs of the FAS1 domains and tried to express them in E. coli. Of them, only single FAS1-II and -IV domains were highly soluble. Circular dichroism (CD) and nuclear magnetic resonance (NMR) studies revealed that the FAS1-IV domain might be suitable for three-dimensional structure determination using NMR spectroscopy.

• 대한바이러스학회지
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• 제28권4호
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• pp.295-302
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• 1998

The RNA genome of poliovirus encodes a long polyprotein precursor and this polyprotein is cleaved proteolytically by viral protease to yield mature proteins. The mature proteins derived from the P1 polyprotein precursor are the component of capsids. To further delineate the process of capsid assembly and encapsidation, in a first attempt, a cell line which expresses the authentic P1 polyprotein was established. CV-1 cells were transfected with the pRCRSVS1P1 plasmid DNA which contains 5'ncr sequences, whole authentic capsid gene of poliovirus and neomycin resistance gene. These cells were treated with G418 for 3 months, and eventually G418 resistant cells were selected and formed colonies. Each colony was picked and grown in the media containing G418. DNA analysis indicated that 1 of 13 neomycin resistant cell lines (R2-18) contains whole poliovirus P1 capsid gene segment which was incorporated into the genome. Immuneprecipitation of cell lysates with sera from rabbit immunized with inactivateded Sabin type 1 particles demonstrated the constitutive expression of the poliovirus P1 capsid protein from R2-18.