• 제목/요약/키워드: complete genome

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사람 수술후상악낭종 병소에서 분리한 Parvimonas micra KCOM 1037의 유전체 염기서열 완전 해독 (Complete genome sequence of Parvimonas micra KCOM 1037 isolated from human postoperative maxillary cyst lesion)

  • 박순낭;임윤경;신자영;노한성;임관주;국중기
    • 미생물학회지
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    • 제55권2호
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    • pp.149-151
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    • 2019
  • Parvimonas micra는 그람 양성, 절대 혐기성, 비운동성 및 아포를 생성하지 않는 구균이다. 이 세균 종은 구강의 정상 세균 총 하나이며, 구강 감염성질환 및 전신질환고도 연관이 있다. P. micra KCOM 1037 (= ChDC B276) 균주가 수술후상악낭종 병소에서 분리되었다. 여기에서 P. micra KCOM 1037 균주의 유전체 염기서열을 완전 해독하여 보고한다.

사람 치근단 농양에서 분리된 Prevotella denticola KCOM 1525의 유전체 염기서열 완전 해독 (Complete genome sequence of Prevotella denticola KCOM 1525 isolated from human periapical abscess)

  • 임윤경;박순낭;박세호;신자영;노한성;국중기
    • 미생물학회지
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    • 제55권2호
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    • pp.152-153
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    • 2019
  • Prevotella denticola는 그람 음성, 절대 혐기성, 비운동성이면서 아포를 형성하지 않는 막대 모양의 세균이다. P. denticola는 치주질환과 관련이 있으며, 치주질환의 위험 인자 중 하나이다. P. denticola KCOM 1525 (= ChDC B698) 균주가 사람 치근단 농양에서 분리되었다. P. denticola KCOM 1525 균주의 유전체 염기서열을 완전 해독하여 보고한다.

Complete genome sequence of Limosilactobacillus fermentum JNU532 as a probiotic candidate for the functional food and feed supplements

  • Bogun Kim;Ziayo Meng;Xiaoyue Xu;Seungwoo Baek;Duleepa Pathiraja;In-Geol Choi;Sejong Oh
    • Journal of Animal Science and Technology
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    • 제65권1호
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    • pp.271-274
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    • 2023
  • Lactic acid bacteria (LAB) have been reported to possess various beneficial properties and are commonly used as probiotics. LAB play a crucial role in milk fermentation, industrial lactic acid fermentation, and health and medicine. Limosilactobacillus fermentum isolated from fermented dairy and food products is considered as 'Generally Recognized as Safe' by FDA. Limosilactobacillus fermentum plays an important role in modulation of the intestinal microbiota, enhancing the host immune system and improving feed digestibility. We isolated a probiotic candidate that was identified and named Limosilactobacillus fermentum JNU532. In a previous report, cell-free culture of L. fermentum JNU532 exhibited anti-melanogenic and antioxidant activities. In this study, we present the complete genome assembly of the bacterial strain JNU532. The final genome consists of one circular chromosome (2,077,416 base pairs) with a guanine + cytosine (GC) ratio of 51.5%.

Complete genome sequence of Lactococcus taiwanensis strain K_LL004, encoding hydrolytic enzymes of plant polysaccharides isolated from grasshopper (Oxya chinensis sinuosa)

  • Hyunok Doo;Hyeri Kim;Jin Ho Cho;Minho Song;Eun Sol Kim;Jae Hyoung Cho;Sheena Kim;Gi Beom Keum;Jinok Kwak;Sriniwas Pandey;Hyeun Bum Kim;Ju-Hoon Lee
    • Journal of Animal Science and Technology
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    • 제65권3호
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    • pp.679-682
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    • 2023
  • The Lactococcus taiwanensis strain K_LL004 was isolated from the gut of a grasshopper (Oxya chinensis sinuosa) collected from local farm in Korea. L. taiwanensis strain K_LL004 is the functional probiotic candidate with an ability to hydrolyse plant polysaccharides. The complete genome of the L. taiwanensis strain K_LL004 contains one circular chromosome (1,995,099 bp) with a guanine + cytosine (GC) content of 38.8%. Moreover, 1,929 Protein-coding sequence, 19 rRNA genes, and 62 tRNA genes were identified based on results of annotation. L. taiwanensis strain K_LL004 has a gene, which encodes hydrolytic enzymes such as beta-glucosidase and beta-xylosidase, that hydrolyzes plant polysaccharides.

Complete Genome Sequence and Analysis of Carnation Italian Ringspot Virus from Erigeron annuus (L.) Pers. in Korea

  • Chung Youl Park;Da Hyun Lee;Young Ho Jung;JunHyeok Kim;Mi Hyun Lee;Un Seop Shin;Hee Ho Lee;Cho Hee Park;Chae Sun Na
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 추계국제학술대회
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    • pp.47-47
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    • 2020
  • In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.

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엽록체 전장유전체 정보를 이용한 Solanum hougasii 특이적 분자마커 개발 (Development of Solanum hougasii-specific markers using the complete chloroplast genome sequences of Solanum species)

  • 김수정;박태호
    • Journal of Plant Biotechnology
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    • 제47권2호
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    • pp.141-149
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    • 2020
  • Solanum hougasii는 감자 야생종 중의 하나로 다양한 종류의 병원균에 대해 저항성을 가지고 있어 감자 육종에서 중요한 재료로 이용되고 있다. S. hougasii는 이질6배체이나 4배체인 감자와 EBN이 4로 같아 직접적인 교배로 육종에 활용될 수 있다. 본 연구에서는 NGS 기술에 의해 완성된 S. hougasii의 엽록체 전장 유전체(cpDNA)와 이를 다른 Solanum종과의 비교를 통해 개발한 분자마커에 대해 보고하였다. S. hougasii의 전체 cpDNA의 크기는 155,549 bp였으며 그 구조는 다른 Solanum 종과 매우 유사하였다. S. hougasii의 cpDNA와 가지과에 속하는 10개 종의 cpDNA 코딩서열을 이용하여 분석한 계통수에서는 S. hougasii와 S. stoloniferum이 거의 동일한 유전체 구성을 보였으며, 다음으로 S. berthaultii 및 S. tuberosum과 유연관계가 가까운 것으로 확인되었다. S. hougasii와 다른 다섯 종의 Solanum과의 전체 cpDNA 다중 정렬을 통해 S. hougasii 특이적인 다섯 개의 InDel과 43개의 SNP 영역을 구명하였으며 이를 기반으로 최종적으로 PCR을 기반으로 한 네 개의 S. hougasii 특이적 마커를 개발하였다. 본 연구의 결과는 Solanum 종들을 대상으로 한 조금 더 세부적인 진화적 그리고 육종적 측면에서의 연구에 기여를 할 수 있을 것이다.

The Complete Chloroplast Genome Sequence and Intra-Species Diversity of Rhus chinensis

  • Kim, Inseo;Park, Jee Young;Lee, Yun Sun;Joh, Ho Jun;Kang, Shin Jae;Murukarthick, Jayakodi;Lee, Hyun Oh;Hur, Young-Jin;Kim, Yong;Kim, Kyung Hoon;Lee, Sang-Choon;Yang, Tae-Jin
    • Plant Breeding and Biotechnology
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    • 제5권3호
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    • pp.243-251
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    • 2017
  • Rhus chinensis is a shrub widely distributed in Asia. It has been used for traditional medicine and ecological restoration. Here, we report the complete chloroplast genome sequence of two R. chinensis genotypes collected from China and Korea. The assembled chloroplast genome of Chinese R. chinensis is 149,094 bp long, consisting of a large single copy (97,246 bp), a small single copy (18,644 bp) and a pair of inverted repeats (16,602 bp). Gene annotation revealed 77 protein coding genes, 30 tRNA genes, and 4 rRNA genes. A phylogenomic analysis of the chloroplast genomes with 11 known complete chloroplast genomes clarified the relationship of R. chinensis with the other plant species in the Sapindales order. A comparative chloroplast genome analysis identified 170 SNPs and 85 InDels at intra-species level of R. chinensis between Chinese and Korean collections. Based on the sequence diversity between Korea and Chinese R. chinensis plants, we developed three DNA markers useful for genetic diversity and authentication system. The chloroplast genome information obtained in this study will contribute to enriching genetic resources and conservation of endemic Rhus species.

Clindamycin 내성 Bacillus licheniformis 14ADL4의 유전체 염기서열 (Complete genome sequence of Bacillus licheniformis 14ADL4 exhibiting resistance to clindamycin)

  • 정도원;이병훈;이종훈
    • 미생물학회지
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    • 제54권2호
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    • pp.169-170
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    • 2018
  • Clindamycin 내성 Bacillus licheniformis 14ADL4 균주는 된장으로부터 분리되었다. 14ADL4 균주의 유전체분석 결과, GC 함량 45.86%, 약 4.3 Mb 크기의 염색체가 확인되었고, 유전체 상의 lmrA와 lmrB 추정 유전자가 clindamycin 내성에 관여할 것으로 예상된다.

Complete Genome Sequences of Two Clonal Complex 398 Methicillin-Resistant Staphylococcus aureus Strains Isolated from Patients in Korea

  • Gi Yong Lee;Ji Heon Park;Ji Hyun Lim;Soo-Jin Yang
    • 한국미생물·생명공학회지
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    • 제51권1호
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    • pp.132-133
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    • 2023
  • Clonal complex (CC) 398 community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) has emerged worldwide in a variety of livestock animals and humans. We report complete genome sequences of Panton-Valentine leucocidin (PVL) and immune evasion cluster (IEC) gene-positive CC398 MRSA strains isolated from patients in Korea.

Complete Genome Sequence of a Methicillin-Resistant Staphylococcus aureus Sequence Type 72 Strain SA520 Isolated from Korean Hospital

  • Ji Hyun Lim;Gi Yong Lee;Ji Heon Park;Soo-Jin Yang
    • 한국미생물·생명공학회지
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    • 제51권2호
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    • pp.212-213
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    • 2023
  • Once known as a major community-genotype, sequence type (ST) 72 methicillin-resistant Staphylococcus aureus (MRSA) strains have been increasingly identified in hospital-associated infections in Korea. Here, we report the complete genome sequence of SA520 isolated from a patient in Korea.