• 제목/요약/키워드: complete genome

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Codon usage and bias in mitochondrial genomes of parasitic platyhelminthes

  • Le, Thanh-Hoa;Mcmanus, Donald-Peter;Blair, David
    • Parasites, Hosts and Diseases
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    • 제42권4호
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    • pp.159-167
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    • 2004
  • Sequences of the complete protein-coding portions of the mitochondrial (mt) genome were analysed for 6 species of cestodes (including hydatid tapeworms and the pork tapeworm) and 5 species of trematodes (blood flukes and liver- and lung-flukes). A near-complete sequence was also available for an additional trematode (the blood fluke Schistosoma malayensis). All of these parasites belong to a large flatworm taxon named the Neodermata. Considerable variation was found in the base composition of the protein-coding genes among these neodermatans. This variation was reflected in statistically-significant differences in numbers of each inferred amino acid between many pairs of species. Both convergence and divergence in nucleotide, and hence amino acid, composition was noted among groups within the Neodermata. Considerable variation in skew (unequal representation of complementary bases on the same strand) was found among the species studied. A pattern is thus emerging of diversity in the mt genome in neodermatans that may cast light on evolution of mt genomes generally.

Complete Mitochondrial Genome of Brown Marmorated Stink Bug Halyomorpha halys (Hemiptera: Pentatomidae), and Phylogenetic Relationships of Hemipteran Suborders

  • Lee, Wonhoon;Kang, Joongnam;Jung, Chansik;Hoelmer, Kim;Lee, Si Hyeock;Lee, Seunghwan
    • Molecules and Cells
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    • 제28권3호
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    • pp.155-165
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    • 2009
  • The newly sequenced complete mitochondrial genome of the brown marmorated stink bug, Halyomorpha halys($St{\aa}l$) (Hemiptera: Pentatomidae), is a circular molecule of 16,518 bp with a total A+T content of 76.4% and two extensive repeat regions in A+T rich region. Nucleotide composition and codon usage of H. halys are about average when compared with values observed in 19 other published hemipteran mitochondrial genomes. Phylogenetic analyses using these 20 hemipteran mitochondrial genomes support the currently accepted hypothesis that suborders Heteroptera and Auchenorrhyncha form a monophyletic group. The mitochondrial gene arrangements of the 20 genomes are also consistent with our results.

고추장에서 분리된 Bacillus subtilis BS16045의 유전체 서열 분석 (Complete genome sequence of Bacillus subtilis BS16045 isolated from Gochujang)

  • 전새봄;허준;엄태붕
    • 미생물학회지
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    • 제53권1호
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    • pp.55-57
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    • 2017
  • 한국 발효식품의 보존성을 높이기 위한 스타터 균주를 얻기 위하여 고추장에서 Bacillus subtilis BS16045를 분리하였다. B. subtilis BS16045에 대한 유전체 분석을 실시하였으며, G+C 비율이 43.6%인 4,165,121 bp 크기의 염기서열을 얻었다. 또한 이 유전체로부터 항진균 및 항균 활성에 연관이 있는 surfactin, kanosamine, bacillaene, plipastatin, subtilosin A, bacilysin 생산 유전자들을 확인하였다. 이러한 결과들을 통해 B. subtilis BS16045는 장류 제조시설에서 유해균의 오염문제를 해결할 수 있는 스타터로 이용될 수 있을 것으로 보인다.

인디고 생산능이 있는 Yangia sp. TSBP01의 유전체 분석 (Complete genome sequence of an indigo producing strain Yangia sp. TSBP01, isolated from oil-contaminated sediment)

  • 김해선;차선호;석호영;박년호;우정희
    • 미생물학회지
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    • 제54권3호
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    • pp.293-294
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    • 2018
  • 유류 누출 사고로 오염된 해양 갯벌에서 분리된 Yangia sp. TSB01은 인돌을 인디고로 생물전환 할 수 있는 특성이 있다. 미생물에서 인디고는 산화효소에 의해 인돌로부터 인독실을 거쳐 생성된다. Yangia sp. TSBP01의 유전체는 2개의 chromosome과 5개의 plasmid로 구성되어 있으며, 총 5,165,974 bp의 크기에 G + C 함량은 66.5%이다. TSBP01는 인디고를 생성하는데 관여하는 indole oxygenase를 보유하고 있다.

수박에 과실썩음병을 일으키는 Acidovorax citrulli strain KACC17005의 유전체 해독 (Complete genome sequence of Acidovorax citrulli strain KACC17005, a causal agent for bacterial fruit blotch on watermelon)

  • 박혜지;성훈제;설우준;오창식;한상욱
    • 미생물학회지
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    • 제53권4호
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    • pp.340-341
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    • 2017
  • Acidovorax citrulli 병원세균은 수박에 과실썩음병을 일으킨다. 이 논문에서는 A. citrulli strain KACC17005 균주의 완전한 게놈 서열을 분석하여 보고한다. 게놈은 총 5,349,924 bp로 구성되어 있으며 G + C 함량이 68.54%이다. 단백질을 coding하는 유전자가 총 4,520개이고, 이들 중 적어도 15개의 유전자들은 감수성 식물에서 병원성을 증가시키거나 저항성 식물에서 면역반응을 유도하는데 중요한 제3형 effector 단백질을 코딩하고 있다.

사람 수술후상악낭종 병소에서 분리한 Parvimonas micra KCOM 1037의 유전체 염기서열 완전 해독 (Complete genome sequence of Parvimonas micra KCOM 1037 isolated from human postoperative maxillary cyst lesion)

  • 박순낭;임윤경;신자영;노한성;임관주;국중기
    • 미생물학회지
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    • 제55권2호
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    • pp.149-151
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    • 2019
  • Parvimonas micra는 그람 양성, 절대 혐기성, 비운동성 및 아포를 생성하지 않는 구균이다. 이 세균 종은 구강의 정상 세균 총 하나이며, 구강 감염성질환 및 전신질환고도 연관이 있다. P. micra KCOM 1037 (= ChDC B276) 균주가 수술후상악낭종 병소에서 분리되었다. 여기에서 P. micra KCOM 1037 균주의 유전체 염기서열을 완전 해독하여 보고한다.

사람 치근단 농양에서 분리된 Prevotella denticola KCOM 1525의 유전체 염기서열 완전 해독 (Complete genome sequence of Prevotella denticola KCOM 1525 isolated from human periapical abscess)

  • 임윤경;박순낭;박세호;신자영;노한성;국중기
    • 미생물학회지
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    • 제55권2호
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    • pp.152-153
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    • 2019
  • Prevotella denticola는 그람 음성, 절대 혐기성, 비운동성이면서 아포를 형성하지 않는 막대 모양의 세균이다. P. denticola는 치주질환과 관련이 있으며, 치주질환의 위험 인자 중 하나이다. P. denticola KCOM 1525 (= ChDC B698) 균주가 사람 치근단 농양에서 분리되었다. P. denticola KCOM 1525 균주의 유전체 염기서열을 완전 해독하여 보고한다.

Complete genome sequence of Limosilactobacillus fermentum JNU532 as a probiotic candidate for the functional food and feed supplements

  • Bogun Kim;Ziayo Meng;Xiaoyue Xu;Seungwoo Baek;Duleepa Pathiraja;In-Geol Choi;Sejong Oh
    • Journal of Animal Science and Technology
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    • 제65권1호
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    • pp.271-274
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    • 2023
  • Lactic acid bacteria (LAB) have been reported to possess various beneficial properties and are commonly used as probiotics. LAB play a crucial role in milk fermentation, industrial lactic acid fermentation, and health and medicine. Limosilactobacillus fermentum isolated from fermented dairy and food products is considered as 'Generally Recognized as Safe' by FDA. Limosilactobacillus fermentum plays an important role in modulation of the intestinal microbiota, enhancing the host immune system and improving feed digestibility. We isolated a probiotic candidate that was identified and named Limosilactobacillus fermentum JNU532. In a previous report, cell-free culture of L. fermentum JNU532 exhibited anti-melanogenic and antioxidant activities. In this study, we present the complete genome assembly of the bacterial strain JNU532. The final genome consists of one circular chromosome (2,077,416 base pairs) with a guanine + cytosine (GC) ratio of 51.5%.

Complete genome sequence of Lactococcus taiwanensis strain K_LL004, encoding hydrolytic enzymes of plant polysaccharides isolated from grasshopper (Oxya chinensis sinuosa)

  • Hyunok Doo;Hyeri Kim;Jin Ho Cho;Minho Song;Eun Sol Kim;Jae Hyoung Cho;Sheena Kim;Gi Beom Keum;Jinok Kwak;Sriniwas Pandey;Hyeun Bum Kim;Ju-Hoon Lee
    • Journal of Animal Science and Technology
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    • 제65권3호
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    • pp.679-682
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    • 2023
  • The Lactococcus taiwanensis strain K_LL004 was isolated from the gut of a grasshopper (Oxya chinensis sinuosa) collected from local farm in Korea. L. taiwanensis strain K_LL004 is the functional probiotic candidate with an ability to hydrolyse plant polysaccharides. The complete genome of the L. taiwanensis strain K_LL004 contains one circular chromosome (1,995,099 bp) with a guanine + cytosine (GC) content of 38.8%. Moreover, 1,929 Protein-coding sequence, 19 rRNA genes, and 62 tRNA genes were identified based on results of annotation. L. taiwanensis strain K_LL004 has a gene, which encodes hydrolytic enzymes such as beta-glucosidase and beta-xylosidase, that hydrolyzes plant polysaccharides.

Complete Genome Sequence and Analysis of Carnation Italian Ringspot Virus from Erigeron annuus (L.) Pers. in Korea

  • Chung Youl Park;Da Hyun Lee;Young Ho Jung;JunHyeok Kim;Mi Hyun Lee;Un Seop Shin;Hee Ho Lee;Cho Hee Park;Chae Sun Na
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 추계국제학술대회
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    • pp.47-47
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    • 2020
  • In this study, we aimed to study carnation italian ringspot virus (CIRV) in Erigeron annuus (L.) Pers. in Bonghwa County, Korea. The collected samples showed mosaic and malformation symptoms. To identify the virus species, we performed high-throughput sequencing, reverse transcription polymerase chain reaction, and cloning. The virus was confirmed to be an unreported species, and therefore we performed genome sequencing of the samples. The complete genome was 4,746 nucleotides in length. The CIRV contained five open reading frames (ORFs), and it showed the typical features of members of the genus Tombusvirus. Phylogenetic analyses revealed that ClRV isolates had the highest nucleotide identities with the CZ isolate (95.89%) from Korea. In recent years, these viruses have sporadically been reported in floral scent and medicinal plants. This research found the first natural host infected with CIRV, and provides baseline information to determine the correlation between weeds and crops.

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