• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.1
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• pp.112-122
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• 2020

This study investigated some enzymatic properties and bitterness improvement of an aminopeptidase retentate fraction (ARF) from common squid Todarodes pacificus hepatopancreas extract (HPE), obtained by ultrafiltration with a 10 kDa molecular weight cut off membrane. Endoprotease and aminopeptidase (AP) activity, and the purity of the ARF (>10 kDa) increased by 6.69-18.11 U/mg and 1.5-2.6 fold, respectively, compared to HPE (2.63-9.37 U/mg). The AP activity toward LeuPNA was stable at 20-55℃ and pH 5-9, but decreased slightly with increasing concentration of NaCl in the reaction mixture. The ARF was the most active MetPNA and preferentially hydrolyzed Glu, Leu and AlaPNA. The bitterness tryptic casein hydrolysates (BTCHs) were treated with ARF, and the bitterness of ARF-BTCHs significantly decreased with increasing amounts of released amino acids Ala, Val, Met, Ile and Leu, which show strong correlations with bitterness. Therefore, the ARF of T. pacificus HPE obtained by ultrafiltration may have a considerable potential for application in protein hydrolysis and appears to be ideally suited to the purpose of lowing bitterness in protein hydrolysates.

• Ocean and Polar Research
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• v.34 no.2
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• pp.253-264
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• 2012

In the northwestern Pacific, spawning of the common squid, Todarodes pacificus, occurs at continental shelf and slope areas of 100-500 m, and the optimum temperature for the spawning and survival of paralarvae is assumed to be $18-23^{\circ}C$. To predict the spawning ground of Todarodes pacificus under future climate conditions, we simulated the present and future ocean circulations, using an East Asia regional ocean model (Modular Ocean Model, MOM version3), projected by two different global climate models (MPI_echam5, MIROC_hires), under an IPCC SRES A1B emission scenario. Mean climate states for 1990-1999 and 2030-2039 from 20th and 21th Century Climate Change model simulation (from the IPCC 4th Assessment Report) were used as surface conditions for simulations, and we examined changes in spawning ground between the 1990s and 2030s. The results revealed that the distribution of spawning ground in the 2030s in both climate models shifted northward in the East China Sea and East Sea, for both autumn and winter populations, compared to that of the 1990s. Also, the spawning area (with $1/6^{\circ}{\times}1/6^{\circ}$ grid) in the 2030s of the autumn and winter populations will decline by 11.6% (MPI_echam5) to 30.8% (MIROC_hires) and 3.0% (MPI_echam5) to 18.2% (MIROC_hires), respectively, from those of the 1990s.

• Journal of the Korean Society for Marine Environment & Energy
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• v.13 no.1
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• pp.68-74
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• 2010

Octopus(Octopus minor), benthic cephalopod, were collected from intertidal zone in Seosan, Choongnam and analyzed for polybrominted diphenyl ethers(PBDEs). PBDEs concentrations ranged from 29 to 109 ng/g lipid wt(mean; 54 ng/g) in mantle and from 12 to 89 ng/g lipid wt(mean; 48 ng/g) in internal organ. PBDEs concentrations in octopus internal organ were lower about two times than that in common squid collected in Yellow Sea, indicating Seosan is relatively less contaminated with PBDEs. Major congener was BDE 206, occupying 72% and 49% of total PBDE concentration in mantle and in internal organ, respectively, which is very different from PBDE composition in common squid with major congeners of BDE 47 and 99. From PBDE composition, octopus seemed to concentrate higher brominated BDE rather than lower brominated BDE. This PBDE composition in octopus may be affected by sediment with extremely high contribution of deca-BDE to total PBDE concentration.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.30 no.5
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• pp.903-905
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• 1997

Morphological differences were studied using the analysis of covariance between various partial length and mantle length of the common Todarodes pacificus by cohorts in the Korean waters. Analysis of seven morphological characters proved that each cohort has no sexual differences, except significant sexual differences only in the Summer cohort in term of relative growth between mantle length and body weight. The three cohorts represent significant differences in morphological characters.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.54 no.6
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• pp.849-860
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• 2021

This study investigated the preparation of seasoned anchovy sauce (SAS) and its functional characteristics by using aminopeptidase active fractions (AAFs) derived from squid Todarodes pacificus hepatopancreas as a bitter taste improver. As the base of the SAS, a hydrolysate (AAAH) prepared by continuously treating raw anchovies with Alcalase-AAF was used. The high-performance liquid chromatography profile of the AAAH suggested that the action of AAFs decreased the hydrophobicity of the N-terminal peptide related to bitterness in the protein hydrolysates. SAS was prepared by blending with the AAAH and other ingredients. The crude protein (2.5%), carbohydrates (18.4%), amino acid-nitrogen (1,325.1 mg/100 mL), and total free and released amino acids (FRAAs, 700.2 mg/100 mL) of SAS were higher than those of commercial anchovy sauce (CAS). Sensory evaluation revealed that SAS was superior to CAS in flavor, color, and taste. The main FRAAs of SAS were glycine (16.8%), alanine (13.2%), glutamic acid (7.8%), and leucine (7.3%). The amino acids that had a major influence on the taste according to the SAS taste values were glutamic acid, aspartic acid, alanine, and histidine. The angiotensin-converting enzyme inhibitory (2.21 mg/mL) and antioxidant activities (3.58 mg/mL) of SAS were superior to those of CAS.

• Journal of Aquaculture
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• v.12 no.2
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• pp.107-114
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• 1999

This study was conducted to evaluate the possible utilization and the replacing range of fish meal analogue (FMA) as a dietary animal protein source for fish meal replacer in fingerling common carp, Cyprinus carpio. Leather meal, meat and bone meal, feather meal, squid liver powder, poultry by product meal, blood meal and amino acids were selected as ingredients for FMA. fish averaging 12.5 g were fed one of five isonitrogenous and isocaloric diets containing fish meal and/or FMA as the dietary animal protein sources. Fish meal protein (0, 20, 40, 60 or 100%) was replaced by the graded level of FMA protein. The feeding trial was conducted for 12 weeks after one week of conditioning period. Percent weight gain of fish fed diets containing 20%, 40% and 60% FMA were not significantly different from that of the fish fed the control diet (P>0.05). Feed conversion ratio of fish fed diets containing 20%, 40%, 60% and 100% FMA were not significantly different from that of fish fed control diet. These findings suggest that replacement of fish meal protein by FMA could be possible up to 60% of fish meal protein in fingerling Israeli carp diets.

• Journal of the Korean Society of Food Science and Nutrition
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• v.19 no.2
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• pp.168-174
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• 1990

유럽화살오징어를 빙장된 상태로 실온(2$0^{\circ}C$)에 저장하여 두고 가공수율 및 저장중 선도변화를 관능적 및 화학적 방법으로 조사한 결과 시료동체에 대한 가식부 수율은 체장이 보통(25-27cm)일 때는 60% 정도였으며 대형(27cm이상) 일때는 64%정도였고 시료전체에 대한 가식부수율은 75-80%였다 실온에서 저장하는 동안 저장 10-12일까지는 식용가능하였고 이때의 관능적 현상으로는 지느러미는 분홍색 동체는 황색반점 흡반은 적색, 육질 견고도는 약한 상태였다 또한 이때의 panel score는 5-6사이였으며 VBN함량은 30-40mg/100g TMA함량은 12-13mg/100g이었고 K값은 관능검사 결과와 잘일치하지 않았다 따라서 유럽화살오징어의 가공원료로서의 저장한계기간은 10일이 적당하다고 판단되었다.

• Journal of Marine Life Science
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• v.4 no.1
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• pp.14-21
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• 2019

This review paper discussed the decadal fluctuations in the catch of the common squid, Todarodes pacificus (T. pacificus) by focusing on migration and distribution patterns. Since 1980s, changes in T. pacificus catches were due to climate regime shift in Korean waters. Fluctuation patterns of catches were different between the East Sea and the Yellow Sea. Generally PDO (Pacific Decadal Oscillation) phase shows a negative correlation with strength of warm current to the East Sea. In 1980s when PDO was positive phase (+), T. pacificus catch was higher in the Yellow, but it was lower in the East Sea. In 1990s when PDO was negative phase (-), T. pacificus catch showed opposite trend compared with 1980s. Such spatial and decadal fluctuations of T. pacificus catch were due to its northward migration along with the warm current or southward movement against the current. In the East Sea, strong (weak) warm current period, the current path has been shifted toward the East Sea coast of Korea (central East Sea or the coast of Japan). It has a correlation with PDO. In the positive PDO phase (1980s), the fishing ground was located on the eastern side of Ulleungdo, whereas during negative PDO phase (1990s), they were situated near the southeastern coast of the Korean peninsula. In the 1980s, volume transport passing into the Yellow Sea increased, whereas volume transport in the East Sea decreased. This is one of major reason increasing T. pacificus larvae in the Yellow Sea.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.3
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• pp.380-385
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• 1998

This study evaluated the possible utilization and the replacing range of animal by-product mixture (ABPM) as a dietary fish meal replacer in growing common carp (Cyprinus carpio). ABPM is a mixture of leather meal, meat and bone meal, blood meal and squid liver powder at a specific weight based ratio. Five different diets were formulated on isonitrogenous and isocaloric basis of $40\%$ crude protein and 15.3 KJ/g diet in which white fish meal (WFM) protein was substituted with the ABPM Protein as follows: diet 1, $100\%$ WFM ($0\%$ ABPM, control); diet 2, $75\%$ WFM+$25\%$ ABPM ($25\%$ ABPM); diet $3,\;50\%$ WFM+$50\%$ ABPM ($50\%$ ABPM); diet 4 $25\%$ WFM+$75\%$ ABPM ($75\%$ ABPM); diet $5.0\%$ WFM+$100\%$ ABPM ($100\%$ ABPM). As the dietary protein sources, each diet contained $34.7\%$ of animal protein supplied by white fish meal and/or ABPM and $65.3\%$ of plant protein. After one week of conditioning period, fish averaging 10 g were randomly assigned to each diet treatment as triplicate groups and fed one of the experimental diets for 12 weeks. Weight gain of fish fed diet 1 (control) and 3 were significantly higher than those of fish fed diet 2, 4 and 5 during the first 4 weeks (P< 0.05), while there were no significant differences among all diet groups during the third 4 weeks (P>0.05). Feed conversion ratio of fish fed diet 1 was significantly higher than those fed diet 2, 3, 4 and 5 during the second 4 weeks (P<0.05), while there were no significant differences among all diet groups during the first and the third 4 weeks (P<0.05). There were no significant differences in proximate analysis among fish fed the experimental diets either for the second 4 weeks or the third 4 weeks (P>0.05). These results indicated that ABPM could be used as a fish meal replacer up to $100\%$ in growing common carp.

• Journal of the Korean Society of Marine Environment & Safety
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• v.17 no.4
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• pp.367-373
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• 2011

We investigated the biological characteristics of the squid, Todarodes pacificus Steenstrup through the samples caught monthly in the Yellow Sea($32^{\circ}$ 30'N, $122^{\circ}E-37^{\circ}$ 30'N, $127^{\circ}E$) from 1985 to 1987. Monthly mantle length frequency were separated into Autumn spawning group and winter spawning group with Tanaka's method(1956) to determine the main group of the squids caught in the Yellow Sea. The ratio of winter spawning group was higher than that of autumn spawning group. Monthly gonadosomatic index of females increased on July in 1985, on October in 1986, and October in 1987 and decreased later in each year. That of males increased on June in 1985, on July in 1986, and June in 1987. The length of 50% group maturity of female was 20.7 cm mantle length(ML) in 1985, 17.9 cm ML in 1986, and 17.2 cm ML in 1987. The ratio of female was higher than that of male in the group over 24 cm ML, and the ratio of male was higher than that of female in the group under 24 cm ML. We concluded that the length of 50% group maturity of squids was affected by the density of the population status.