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Development of Lateral Flow Immunoassay for Antigen Detection in Human Angiostrongylus cantonensis Infection

  • Chen, Mu-Xin;Chen, Jia-Xu;Chen, Shao-Hong;Huang, Da-Na;Ai, Lin;Zhang, Ren-Li
    • Parasites, Hosts and Diseases
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    • 제54권3호
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    • pp.375-380
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    • 2016
  • Angiostrongyliasis is difficult to be diagnosed for the reason that no ideal method can be used. Serologic tests require specific equipment and are not always available in poverty-stricken zone and are time-consuming. A lateral flow immunoassay (LFIA) may be useful for angiostrongyliasis control. We established a LFIA for the diagnosis of angiostrongyliasis based on 2 monoclonal antibodies (mAbs) against antigens of Angiostrongylus cantonensis adults. The sensitivity and specificity were 91.1% and 100% in LFIA, while those of commercial ELISA kit was 97.8% and 86.3%, respectively. Youden index was 0.91 in LFIA and 0.84 in commercial ELISA kit. LFIA showed detection limit of 1 ng/ml of A. cantonensis ES antigens. This LFIA was simple, rapid, highly sensitive and specific, which opened an alternative approach for the diagnosis of human angiostrongyliasis.

노동심판제도의 효율성 평가 (The Efficiency of the Korean System of Lobor Adjudication)

  • 김태기
    • 노동경제논집
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    • 제28권1호
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    • pp.83-104
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    • 2005
  • 노동위원회의 심판제도는 부당해고사건과 부당노동행위사건을 신속하게 해결한다는 점에서 분쟁해결의 중요한 장점을 가지고 있지만 여러 가지 비판을 받고 있다. 본 논문은 노동위원회 심판기능의 효율성을 실증적으로 분석하고 노사분쟁을 보다 효과적으로 해결할 수 있는 방안을 제시한다. 분석결과는 노동심판제도에 대한 불만이 분쟁해결의 결과보다는 분쟁해결의 과정에 기인하고 있다는 점을 보여준다. 또한 노동심판제도의 문제점도 노사 당사자의 의식이나 태도보다는 노동위원회의 위원이나 심사관의 자세나 자질 등에 기인하는 바가 크다는 점을 보여준다. 이것은 사건에 대한 노동위원회의 조사활동이 보다 충실하게 이루어져야 하고, 노사 당사자가 자신의 주장을 충분히 뒷받침할 수 있도록 심문회의의 진행방식과 위원들의 심문태도가 개선되어야 하며, 판정을 하는 경우 그 내용이 실효성을 가질 수 있도록 적절해야 하며, 판정이 존중받을 수 있도록 법적 판단에 대한 권위도 확보해야 한다는 점을 시사한다.

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볼락 방류사업의 현황 및 과제 - 경제효과분석을 중심으로 - (The Current Status and Tasks of Rockfish Restocking Project based on Economic Performance Evaluation)

  • 송정헌;홍재범
    • 수산경영론집
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    • 제40권3호
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    • pp.69-88
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    • 2009
  • This research was as part of restocking project conducted until 1986. The necessity of its economic and scientific verification on restocking project increased. The economic analysis of this project was intended to measure its effectiveness of rockfish restocking throughout certain water areas. The rockfish restocking in 2008 was carried two regional governments of Gyeongnam and Busan. 3-year average annualized performance is 3.76 million rockfish restocked and 940 million won invested. As Gyeongnam restocked 3.62 million rockfish and invested 890 million won, most of rockfish stocking accomplished in Gyeongnam. The public shipment was the 3-year average amount was about 400 tons. Each Suhyup shipment as follows; TongYong 66.6 tons, Namhae 23.6 tons, Samcheonpo 17.2 tons; Yeosu 13.3 tons, Geoje 4.7 tons, Goseong 3.1 tons, Hadong 1.0 tons. The private shipment was assessed by the interview of relevant market participations. The high percent areas of public ship were Nemaha, Goseong 90% and Geoje 80% or more. Hadong and Samcheonpo were about 50%. TongYong was 10% and the lowest. The private shipment was calculated using the percentage of private shipment surveyed. The total amount of private shipment was 4.8 billion, where 4.5 billion was TongYong, where most of private shipment is being made. The positive economic benefit occurred when the percent of restocked is over 15% in the overall catch. The percentage of restocked investigated with genetic test was 58.2%. With 58.2% ratio of restocked, there was 3.8 times economic benefit according to approximately 3.6 billion won. Economic effects were highly significant. As catch increased according restocking, joint market commission increased. Joint market commission was calculated as total restocked shipment multiplied by the ratio of commission, the calculated commissions were as follows; Hadong 5.0%, Geoje 4.8%, Goseong 4.8%, Namhae 4.8%, Samcheonpo 4.3%, Yeosu 4.3%, TongYong 4.0%. According to calculation results, annual commission fee of ₩60,000,000 was raised during 2006~2008. The fishing catch bulk for entertainment significantly increased in sea area carried with mass restock.

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Electrochemical Investigation of Tryptophan at a Poly(p-aminobenzene sulfonic acid) Film Modified Glassy Carbon Electrode

  • Ya, Yu;Luo, Dengbai;Zhan, Guoqin;Li, Chunya
    • Bulletin of the Korean Chemical Society
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    • 제29권5호
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    • pp.928-932
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    • 2008
  • A glassy carbon electrode (GCE) modified with poly(p-aminobenzene sulfonic acid) [Poly(p-ABSA)] film is fabricated by voltammetric technique in phosphate buffer solution (pH 8.0) containing $5.0\;{\times}\;10^{-3}\;mol\;L^{-1}$p- ABSA. Electrochemical behaviors of tryptophan at the Poly(p-ABSA) film electrode are investigated with voltammetry. The results indicate that the electrochemical response of tryptophan is improved significantly in the presence of poly(p-ABSA) film. Compared with the bare glassy carbon electrode, the Poly(p-ABSA) film electrode remarkably enhances the irreversible oxidation peak current of tryptophan. Some parameters such as voltammetric sweeping segments for the electrochemical polymerization, pH, accumulation potential and accumulation time are optimized. Under the optimal conditions, the oxidation peak current is proportional to tryptophan concentration in the range of $1.0\;{\times}\;10^{-7}$ to $1.0\;{\times}\;10^{-6}\;mol\;L^{-1}$, and $2.0\;{\times}\;10^{-6}$ to $1.0\;{\times}\;10^{-5}\;mol\;L^{-1}$ with a detection limit of $7.0\;{\times}\;10^{-8}\;mol\;L^{-1}$. The proposed procedure is successfully applied to the determination of tryptophan in a commercial amino acid oral solution.

민사조정의 활성화와 사적자치 (A study on the private autonomies of the disputants in the process of conciliation)

  • 주인
    • 한국중재학회지:중재연구
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    • 제13권2호
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    • pp.613-630
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    • 2004
  • Conciliation is one of the most effective ADR(alternative dispute resolution) which takes the place of civil procedure. It is achieved with disputants' independent will. The disputants negotiate each other, and make peaceful settlement. If a compromise is effected between the two, it regards the compromise as a judgement of the Supreme Court. This effect on the conciliation is afford a basis for the private autonomies. But nowadays, the practical use of the private autonomies is not thoroughgoing enough in our country. It is a matter of no uncommon occurrence for the member of a conciliation commission to form a conclusion about the dispute and to persuade the disputants to accept the conclusion. Even the judges have a tendency to conduct a conciliation like civil procedure. Under these circumstances, it's harsh to the disputants that a compromise in the conciliation has an effect like the judgement of the Supreme Court. So you should reconsider carefully the role or service of a conciliation commission. The role of a conciliation commission must be to guarantee an atmosphere of freedom, and for disputants to negotiate without restraint. So the members of a conciliation commission should make an offer the disputants the information on the members and proceedings of the conciliation. It will make the disputants have a firm belief that the members are fair and conciliation will be progressed in a fair. Moreover they have to notify the disputants of the estimated norms which is concerned in the dispute, too. It will facilitate the negotiation and compromise, and will justify claim preclusion(res judicata) which is based on Korean Civil Conciliation Law(Article 29) says that conciliation has the full force and effect of a civil judgement of the Supreme Court.

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방송통신위원회 설립 과정의 특성 분석 : 옹호연합의 전략적 행동을 중심으로 (Analysis of the Korea Communications Commission establishment process : Focusing on Strategic behavior of Advocacy coalition)

  • 김상운
    • 디지털융복합연구
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    • 제15권7호
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    • pp.81-90
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    • 2017
  • 본 연구는 2008년 방송통신위원회 설립 과정에서 방송 옹호연합과 정보통신 옹호연합의 전략적 행동을 분석하여 정부조직개편 과정에 대한 시사점을 제시하고자 하였다. 이를 위해 먼저 방송통신융합기구 논의가 왜 장기적인 경쟁과 대립을 겪었는지를 파악하기 위해 방송통신융합정책의 특성을 분석하였다. 방송통신융합정책은 방송 영역과 정보통신 영역간 공익성과 산업성이라는 상충된 이념과 이해관계가 존재하고 있어 장기간 대립 상황에 놓일 수밖에 없었다. 두 영역은 기구개편 논의 과정에서 옹호연합을 형성하여 유리한 결과를 얻기 위하여 전략적 행동을 취했고, 개편 논의는 두 옹호연합간 전략적 행동이 어우러지며 진전되고 있었다. 그러나 정권 교체와 함께 기존의 논의는 무색해지고 개편안은 정치적 협상에 의해 마무리되었다. 정치 논리에 의해 설립된 방송통신위원회는 이후 정치권에 의해 좌지우지되는 모습을 보이며 정책 실패 사례를 양산하며 조직 개편의 부작용을 드러냈다. 이러한 부작용을 최소화하기 위해 조직개편 과정에서 정치성을 최소화하는 제도적 장치의 마련이 필요하며 무엇보다 조직개편을 정치 도구로 사용하는 관행을 극복하는 노력이 필요하다.

The Magas1 Gene is Involved in Pathogenesis by Affecting Penetration in Metarhizium acridum

  • Cao, Yueqing;Zhu, Xiangxian;Jiao, Run;Xia, Yuxian
    • Journal of Microbiology and Biotechnology
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    • 제22권7호
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    • pp.889-893
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    • 2012
  • Appressorium is a specialized infection structure of filamentous pathogenic fungi and plays an important role in establishing a pathogenic relationship with the host. The Egh16/Egh16H family members are involved in appressorium formation and pathogenesis in pathogenic filamentous fungi. In this study, a homolog of Egh16H, Magas1, was identified from an entomopathogenic fungus, Metarhizium acridum. The Magas1 protein shared a number of conserved motifs with other Egh16/Egh16H family members and specifically expressed during the appressorium development period. Magas1-EGFP fusion expression showed that Magas1 protein was not localized inside the cell. Deletion of the Magas1 gene had no impact on vegetative growth, conidiation and appressorium formation, but resulted in a decreased mortality of host insect when topically inoculated. However, the mortality was not significant between the Magas1 deletion mutant and wild-type treatment when the cuticle was bypassed by injecting conidia directly into the hemocoel. Our results suggested that Magas1 may influence virulence by affecting the penetration of the insects' cuticle.