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Genotypic Identification in Commercial Strains of Pleurotus ostreatus based on AFLP and VCGs (AFLP 분석 및 체세포 불화합성에 의한 느타리 유사품종의 확인)

  • Seo, Kyoung-In;Yoo, Young-Bok;Jang, Kab-Yeul;Shin, Pyung-Gyun;Oh, YounLee;Kim, Kwang-Ho;Kong, Won-Sik
    • The Korean Journal of Mycology
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    • v.41 no.1
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    • pp.14-20
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    • 2013
  • We already reported four groups which contains some similar strains based on URP-PCR in the previous paper. The objective of this study was to confirm those strains by the amplified fragment length polymorphism (AFLP) and vegetative compatibility group (VCG). AFLP analysis showed no difference among these strains except ASI 2595 and 2183 in Weonhyeong group and ASI 2829 in Suhan group. They showed specific DNA bands only in the result of P + AG/M + AAG and P + GT/M + ATG primer combinations out of eight different combinations. The AFLP primers produced a total of 330 fragments between 80 and 1000 bp in length for 31 Pleurotus ostreatus strains. At a genetic similarity of 0.96, the UPGMA analysis separated the isolates into four distinct clusters. Each group was classified by similar strains. Confrontation test by vegetative compatibility groups (VCGs) also showed distinct line between strains from different groups, but no line between similar strains within the cluster. Our results indicate that most of similar strains was not distinctness. Thus, similar strains are considered to be very close on the genealogy of their parent or same strain with different name.

A Historical Survey on the Background of Establishment of British P & I Club (영국계 P&I 클럽의 설립배경에 관한 사적 고찰)

  • Shin, Gun-Hoon
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.34
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    • pp.77-108
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    • 2007
  • The traditional name given to the insurance of third party liabilities and certain contractual liabilities which arise in connection with the operation of ships is protection and indemnity(P & I) insurance. P & I insurance is very different from traditional hull and machinery insurance in that shipowners' hull and machinery insurance is designed primarily to protect the assured against losses to his vessel, whereas P & I insurance seeks to indemnify an shipowner in respect of the discharge of legal liabilities he has incurred in operating his own vessels. This study is to examine the background of establishment of British P & I clubs md, therefore, the identity of P & I insurance. The present British P & I clubs are the remote descendants of the many small and local hull mutual insurance clubs that were formed by British shipowners in the end of 18th century. At that time, British shipowners were dissatified with the state of marine insurance market and, therefore, established clubs together in mutual hull insurance clubs. After the removal of the company monopoly in 1824, greater competition had a good effect on the rates, terms of cover and service offered by the commercial marine insurance market and by Lloyd's underwriters, and the hull clubs became less necessary and went into decline. The burden of British shipowners on liabilities to third parties was steadily increased after the middle of the 19th century, but the amount insured under hull policy was limited in the insured value of the ship. Eventually, the first protection club, that is, the Shipowners' Mutual Protection Society was formed in 1855. It was designed to like past mutual hull clubs, but to cover liabilities for loss of life and personal injury and also the collision risks excluded from the current marine policies, particularly the excess above the limits in hull policies. In 1870, the risks of liability for loss of or damage to cargo carried on board the insured ship was first awarded by the British shipowners. After 1874, many protection clubs formed indemnity club to cover the risk of liability for loss or damage to cargo. As mentioned above, British P & I clubs have been steadily changed according to the response of shipowners under the rapidly changing law of British shipowners' liability, and so on in the future.

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The amount of dentifrice used by opening diameter size and current status of commercial dentifrices for children in Korea (치약 튜브 입구 직경에 따른 치약사용량 및 국내 시판 어린이치약의 현황)

  • Chun, Ju-Yeon;Lee, Hyun-Ok;Kang, Young-Hee
    • Journal of Korean society of Dental Hygiene
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    • v.10 no.3
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    • pp.541-553
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    • 2010
  • Objectives : The purpose of this study was to examine the use of dentifrice for children, the state of dentifrice for children on the market and the relationship between the bore of dentifrice and the amount of dentifrice used in an effort to provide parents, main consumers of dentifrice for children, with the right information on dentifrice. Methods : Data on the use of children's dentifrice were gathered by investigating parents in Seoul and Gyeonggi Province in July and August, 2009. The selected parents had children aged one to five in Western age. Results : 1. As to the purchase of dentifrice for children, the largest group (75.9%) were aware of the name of the toothbrush used by their children, and the greatest group (97.3%) bought the toothbrush on behalf of them. Advertising was the most dominant source of information on the choice of dentifrice (51.8%), and non-professionals were the most common providers of information on that (55.9%). 2. The amount of dentifrice used at a time was a mean of $0.467{\pm}0.270g$. There was a wide disparity in that aspect from a low of 0.100 to 1.900g. As to the influence of the size of the bore of dentifrice, the greatest amount of dentifrice was used ($0.670{\pm}0.306g$) when the bore of dentifrice was 9 mm in size, and the bore size 9 mm was significantly different from the size 3 mm and 6 mm in that aspect (p<0.001). 3. There was one kind of imported dentifrice for children on the market. Concerning the fluoride ingredient of dentifrice, the biggest number of dentifrice contained MFP only. As to the size of the bore of dentifrice, there was a difference of 3 to 9 mm. Most of dentifrice for children included the pictures of particular characters. Conclusions : The above-mentioned findings illustrated that there is a serious concern for the intake of fluoride among infants and preschoolers, it's needed to pay careful attention to the use of dentifrice. Dentifrice for children should be manufactured in consideration of the relationship between the size of the bore and the amount of dentifrice used.

Comparison Study on the Material Characteristics of Oil Paints (I) (유화물감의 재질적 특성 비교 연구 (I))

  • Kim, Jung Heum;Park, Hye Sun;Lim, Sung Jin
    • Journal of Conservation Science
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    • v.33 no.2
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    • pp.85-95
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    • 2017
  • Oil paints are mixtures of pigments, drying oils and additives. In the past, oil paints were mainly composed of inorganic pigments. However, recently color matching techniques vary depending on manufacturers due to the development of various kinds of synthetic pigments. Despite this, most studies of oil paints in South Korea are about durability tests, and there is no comparative study on the characteristics of commercial oil paint components. This study aims to compare the properties of four different kinds of oil paints from four manufacturers, which are the most popularity used. Extender pigments in oil paint from C brand differed from that of other manufacturers and various kinds of coloring pigments were differently used depending on the oil paints and the manufacturers. The mixing ratios and the pigment types differed even for oil paints having the same product name. It is assumed that these differences could affect the colors. The result of this study is expected to contribute to the analysis of artworks through the accumulation of scientific data of oil paints. In addition, it can be utilized as a scientific basis for art history studies, including the characteristics of artists or production year.

A Study on the Alienation and Inheritance of the Right of Publicity (퍼블리시티권의 양도성과 상속성에 관한 연구)

  • Kwon, Sang-Ro
    • Proceedings of the Korea Contents Association Conference
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    • 2009.05a
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    • pp.532-536
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    • 2009
  • Recently the entertainment and sports/advertisement industry have developed rapidly, and the name or identities of celebrities such as entertainers or athletics for the advertisement so that following troubles are occurred continually. Some celebrities actually started a suit for there right of publicity was violated, and won the case. Meanwhile, the right of publicity has become a growing issue in the society. In the States, the right of publicity which controls using one's identity for commercial use, and it is recognized as a right of property separated from the right of privacy. But in Germany, the right of publicity is protected as a human right. On the other hand, in Korea, there is an argument that the mental human right and the right of publicity which has property-characteristic are not separated clearly, and also the attitude of precedent toward the right of publicity is not unified. Especially in Korea, where it is taking the written laws principally, it is not easy to recognize the right of publicity which is a monopoly and exclusion without basis such as any actual laws such as regulation of agreement or any conventional laws. Therefore, as the right protection of celebrities is becoming the social state and there is an agreement with the constituents of the society, now Korea shall progress legislation about the right of publicity, and prepare a legal basis which regulates the remedies for the realization condition, alienation, inheritance, objectives, duration and violation of the right of publicity.

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The Preliminary Study on the Quantitative Analysis of Quarts by Fourier Transform Infrared Spectrophotometric Direct on Filter(FTIR-DOF) Method -Effects of filter materials, inhomogeneity of deposition, and humidity- (Fourier Transform Infrared Spectrophotometric Direct on Filter 방법을 이용한 석영 분석의 기초 연구 - 필터재질, 비균일 침착 및 습도의 영향 -)

  • Phee, Young Gyu;Kim, Hyunwook
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.15 no.1
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    • pp.1-7
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    • 2005
  • Although the Fourier Transform Infra-Red spectrophotometric Direct on Filter(FTIR-DOF) method is a useful analytical technique for quantifying quartz content in respirable dust samples, a number of analytical problems must be taken into consideration such as, to name only a few, inhomogeneous deposition of particles, level of environmental humidity, uneven surface of the filter, and interfering minerals in the sample. This study was designed to select the most suitable wavelength and proper filter material for the method, and to investigate effects of humidity and inhomogeneous deposition of particles on the filter. Samples of respirable dust, created in a dust chamber containing standard material of quartz, were collected using a cyclone equipped with a 25mm filter as a collection medium. The results were as follows; 1. Among seven (7) commercially available filters tested for the FTIR-DOF method, the DM 800 filter showed the best analytical performance having the lowest background absorbance bands and no overlapping peaks at 799, 779, and $695cm^{-1}$. 2. The variations of absorbance due to humidity ranged from 1.0% to 3.3% for $799cm^{-1}$, 1.0% to 3.3% for $779cm^{-1}$, and 8.9%~20.9% for $695cm^{-1}$ peaks, respectively. The $699cm^{-1}$ peak was proved to be most vulnerble to environmental humidity for quantitative analysis of quartz. 3. As for effects of inhomogeneous deposition of samples, the highest variation of absorbance of 10.9% ($13.5{\mu}g$) was observed when using the 695cm-1. The variations of absorbance from the other two peaks, 799 and $779cm^{-1}$, ranged from 1.2 to 3.2%, and 1.4 to 4.1%, respectively. Therefore, the $799cm^{-1}$ peak was considered to be most reliable for quantitative analysis of quartz. The results of this study suggest that, for quantitative analysis of quartz in the respirable dust samples, use of the $799cm^{-1}$ peak can minimize the influence of environmental humidity and inhomogeneous deposition of particles on the filter. The FTIR-DOF method, if adopted for routine analysis of quartz in the respirable dust samples, could save sample preparation time and efforts substantially and also could increase analytical throughputs. Since use of the $799cm^{-1}$ peak is prone to be affected by interferences in the sample, further research on minimizing the effects is needed.

A Design and Implementation of HTML5 Vector Map for Individual Purpose Service (개인화 지도 서비스를 위한 HTML5 벡터지도 설계 및 구현)

  • Kwon, Jin-Young;Choi, Se-Hyu
    • Spatial Information Research
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    • v.23 no.4
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    • pp.57-66
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    • 2015
  • At these day, owing to functional limitations and cost issues, used image map in web service have a problem which can not make exactly meet the user needs. This study aims to create an individual map for user suitable purposes using HTML5 technology that implement the vector map creation and its functions with services. The results of this study, the invisible problems of the tilting and rotation functions in image-based map utilizing the existing web environment were solved in HTML5 vector map. And to access the map information, by implementing the function of expressing the background and name data to selectively derive, various results were expressed in the map. Also, as a result of a comparison of performance the time required was measured at 0.88sec which comes in the range of the first loading time between 0.78sec and 7.56sec in a commercial map service, which showed the possibility of its commercialization. compared to 180GB of image maps for the production of a national map index system, the volume to produce a vector map was 2.5GB, a decrease by over 90%, which solved the issue of costs for a storage space. As a result, this study of HTML5 vector map design and implementation presented a plan for providing information suitable for the requirements of the users who use spatial information through utilizing a variety of information and expanding functions.

Monitoring of On-Line Nutrition Information-Analysis of Meta Data (인터넷 영양정보의 모니터링-메타데이터의 분석)

  • 강혜경;강명희;유경혜;이선영
    • Journal of Nutrition and Health
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    • v.37 no.8
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    • pp.688-700
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    • 2004
  • This study was conducted to analyze how appropriate the on-line nutrition information was externally as a web information. Four-hundred-ninety-seven web sites from 5 internet search engines (Yahoo, Empas, Nate, Hanmir, Naver) were selected on the basis of April 25th, 2004. The skillful personnels monitored them about 8 evaluating categories: clarity, purpose, authority, durability, advertisement, privacy and/or security, responsibility, and contents. Forty percent of the selected web sites were operated by the companies which had commercial purpose like internet shopping malls and 5.6% by academies, societies, research institutions, schools/colleges and public institutions. Most of web sites (76.1 %) were managed for advertisements and sales of companies' commodities, and 32.6% had the food and nutrition information as first purpose. Ninety-three percent of web sites were targeted to healthy individuals through whole life cycle. Specifically, there were lots of web sites for the obesity which were offered by diet related companies. Of the 497 web sites, 193 mentioned the name providing the nutrition information, but only 1/3 had reliability on their specialty. As a source of nutrition information, 52.7% of web sites were using 'books of the major field' and 42.0% 'newspapers' and 23.7% 'broadcasting', respectively. Most web sites mentioned 'setting-up date' but not 'renewal date'. Thirty-six percent of web sites took '2 - 3 days' for the operators to answer the questions through the bulletin. Forty-seven percent of web sites answered' 1 - 10 questions' per 1 week, but 40.1 % of them didn't answer for a week at all. There were 118 web sites (23.7%) to record the connected frequencies and 36.0% of them put the advertisements. Around 96% of web sites mentioned feedback addresses. Among the menus of web sites, 68.0% were about self-advertisement and 64.0% about nutrition information. Each web site was scored to judge its external quality according to the operators by selecting 13 items. Web sites managed by public institution had highest scores (9.5), and lowest in private vendors', food companies' and individual web pages. Among search engines, Naver got the highest score of 7.0 and Nate the lowest one of 6.1. As it was only the pilot study, there were several limits in evaluating tools, time and monitored quantity. To make monitoring of on-line nutrition informatiions actively, standardized monitoring forms might be developed under the integrated studies.

Allelic Gene Interaction and Anthocyanin Biosynthesis of Purple Pericarp Trait for Yield Improvement in Black Rice (흑미의 자색종자과피 형질을 결정하는 대립유전자와 안토시아닌 생성의 상호관계)

  • Rahman, Md Mominur;Lee, Kyung Eun;Kang, Sang Gu
    • Journal of Life Science
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    • v.26 no.6
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    • pp.727-736
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    • 2016
  • Rice (Oryza sativa L.) is one of the major cereal crops for consumption by the world’s population. Recently, various colored rice, such as white, red, brown, green, and black rice, have caught the attention of world consumers. The commercial name ‘black rice’ contains a high amount of anthocyanins in pericarp, which increases nutritional value. Moreover, anthocyanin in black rice possesses biomedical properties, including anti-oxidant, anti-cancer, and anti-inflammatory effects in humans. In genetics, black rice has a dominant PURPLE PERICARP (Prp) trait governed by two genes, Pb and Pp, which are involved in the synthesis of cyanidin-3-O-glucoside (C3G). Since the publication of a report by Nagai at 1921, the genetics and physiological studies of black rice driven by Prp traits are still unable to understand the relevant genes and their roles. However, with the increased demand for anthocyanin-rich black rice as a functional food for human health, it has become urgent to develop highyielding anthocyanin-rich varieties of rice. We explored many years in the genetics of purple pericarp trait, anthocyanin biosynthesis in pericarp during seed development, and, consequently, their products in relation to different physiological and agronomic traits. In this review, we summarized the anthocyanin biosynthesis in pericarp, emphasizing the inheritance pattern of the trait and functions of their products on different physiological and agronomic traits, including the yield of black rice.

Superconducting Thick Film by Lateral Field Assisted EPD (측면보조전계 인가 전기영동전착 초전도후막)

  • 전용우;소대화;조용준
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.8 no.3
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    • pp.679-685
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    • 2004
  • Although the electrophoretic deposition method has the advantage of simple processing procedure, less fabrication facilities, and easier control for deposition thickness and wire length, providing economical and technical merits, it also has the disadvantages of cracking and porosity phenomena, requiring an improved processing method for higher particle density and constant particle orientation. we have developed an optimization method to increase the particle density and to unify its orientation, and have performed a study to overcome the cracking and porosity problems in the fabricated superconductor. In order to improve the surface uniformity and the conduction properties of the fabricated YBCO thick films, a system that applies alternate voltage vertically has been developed for the first time and applied to the electrophoretic deposition process. The applied alternate electric field caused a force to be exerted on each YBCO particle and resulted in a rotation of the particle in the direction of applied electric field, accomplishing a uniform particle orientation. We name this process as the shaky-aligned electrophoretic deposition method. For commercial utilization and efficiency, in this dissertation, alternating voltage of 60 Hz and 25 ∼ 120 V/cm was proposed to apply it as a subsidiary source for shaky-flow deposition so that the fabricated thin film showed uniform surface morphology with less voids and cracks and Tc,zero of 90 K and the critical current density of 3419 A/$cm^2$.