• Title/Summary/Keyword: commercial ${\alpha}$-cellulose

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Production and Characteristics of Cellulose from Saprolegnia ferax (미생물 셀룰로오스의 생산 및 특성에 관한 연구)

  • Kang, Jin-Ha;Park, Seong-Cheol
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.39 no.3
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    • pp.77-83
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    • 2007
  • This study was carried out to examine the optimum culture condition for the production of cellulose from Saprolegnia ferax and its physical characteristics. Conclusions obtained from the results of this study were as follows: In producing the cellulose from S. ferex, optimal pH and temperature were 7.0 and $26{\sim}30^{\circ}C$ with a maximum of $26^{\circ}C$, respectively. And, optimal culture period was 11days. WHC and OHC of biocellulose were 3.2(25.04 g/g) times and 3.5(25.75 g/g) times higher than those of commercial ${\alpha}-cellulose$(7.57, 7.25 g/g) respectively. The viscosity of biocellulose is lower than that of commercial ${\alpha}-cellulose$. And the effect of rpm on the viscosity of biocellulose was more than on the that of ${\alpha}-cellulose$.

Physicochemical Properties of Fibrous Material Fraction from By-product of Aloe vera Gel Processing (알로에 베라 겔 가공부산물로서의 섬유질 분획의 성분 및 물리화학적 특성)

  • Baek, Jin-Hong;Lee, Shin-Young
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.118-126
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    • 2010
  • The fibrous material fraction as a by-product from the commercial aloe vera gel processing was obtained and freeze dried. The physicochemical characteristics such as the proximate composition, crystalline/surface structures and several physical functionalities including the water holding capacity (WHC), swelling capacity (SW), oil holding capacity (OHC), emulsion/foam properties and viscosity properties of this powdered sample (100 mesh) were investigated and analyzed by comparison with commercial $\alpha$-cellulose as a reference sample. The total dietary fiber content of powdered sample was very high as much as 87.5%, and the insoluble dietary and soluble dietary fiber content ratios were 77.6 and 22.4%, respectively. The FT-IR spectrum of powdered sample showed a typical polysaccharide property and exhibited a x-ray diffraction pattern for cellulose III and IV like structure. SW (8.24${\pm}$0.15 mL/g), WHC(6.40${\pm}$0.19 g water/g solid) and OHC(10.32${\pm}$0.29 g oil/g solid) of freeze dried aloe cellulose were about 3.3, 1.4 and 2 times higher than those of commercial $\alpha$-cellulose, respectively. Aloe cellulose (~2%, w/v) alone had no foam capacity while improved the foam stability of protein solution (1% albumin+0.5% $CaCl_{2}$) by factor of 300%. Emulsion capacity of 2%(w/v) aloe cellulose was about 70% level of 0.5%(w/v) xanthan gum, but its emulsion stability was about 1.2 times higher than that of xanthan gum. Also, aloe cellulose containing CMC (carboxyl methyl cellulose) of 0.3%(w/v) showed a very good dispersity. Aloe cellulose dispersion of above 1%(w/v) exhibited higher pseudoplasticity and concentration dependence than those of $\alpha$-cellulose dispersion, indicating the viscosity properties for new potential usage such as an excellent thickening agent.

Anaerobic Fermentation and Weight of Hydrated Residue of Dietary Fibers in vitro (In Vitro 법에 의한 식이섬유의 혐기적 발효 및 수화 잔여물의 무게)

  • 이경숙
    • Journal of Nutrition and Health
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    • v.28 no.9
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    • pp.834-845
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    • 1995
  • This study was conducted in order to establish an in vitro method simulating the physiological function of fibers along the large intestine of humans. Commercial fibers including guar gum, apple pectin, citrus pectin, CM-cellulose, alginic acid and $\alpha$-cellulose, and dietary fiber residues obtained from rice bran, barley, soybean, Korea cabbage, apple, tangerine and sea mustard were employed to determine the water-holding capacity, weight of hydrated residue and fiber content after anaerobic fermentation using human fecal inoculum for 24 hours, followed by dialysis under osmotic suction pressure. The weight of hydrated residue in commercial fibers was in the decreasing order of CM-cellulose > alginic acid, $\alpha$-cellulose > apple pectin, citrus pectin > guar gum and that in food fiber residues was in the decreasing order of rice bran, sea mustard > soybean > tangerine, Korean cabbage > barley > apple. It was demonstrated that the larger the weight of hydrated residue was, the more the weight of human stool increased. Consequently this in vitro method can be used as a preceeding test before undertaking animal or human experiment to predict the physiological effects of fiber residues from diverse food samples as well as commercially refined fibers.

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Retarding Effect of Dietary Fibers on the Glucose and Bile Acid Movement Across a Dialysis Membrane in Vitro (In Vitro법에 의한 식이섬유의 포도당 및 담즙산 흡수 지연 효과)

  • 이경숙
    • Journal of Nutrition and Health
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    • v.29 no.7
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    • pp.738-746
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    • 1996
  • In order to anticipate the physiological function of dietary fibers, glucose and bile acid retarding effects were experimented by using in vitro methods based on dialysis for commercial fibers and dietary fiber residue of food samples. The glucose retarding effect in commercial fibers increased in the order of alginic acid, guar gum, CM-cellulose, citrus pectin > apple pectin > $\alpha$-cellulose and the effect in food fiber residues increased in the order of sea mustard > Korean cabbage, apple > rice bran, barley, soybean, and tangerine. The bile acid retarding effect in commercial fibers increased in the order of citrus pectin, guar gum > CM-cellulose, alginic acid > apple pectin > $\alpha$-cellulose and the effect in food fiber residues increased in the order of barley, rice bran > sea mustard > tangerine > Korean cabbage, soybean > apple. The higher the retarding effect of glucose movement through the dialysis membrane, the more effective the control of the human blood glucose level. As the retarding effect of bile acid movement across the dialysis membrane increased, the human serum cholesterol level correspondingly reduced. Consequently these in vitro methods can be used as a preceding test before undertaking animal and human experiments to predict the physioloical effects of fiber residues from diverse food samples as well as commercially refined fibers.

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Reaarding Effect of Dietary Fibers Isolated from Tangerine Peels on Glucose, Bile Acid, Cadmium transport In Vitro (감귤과피로부터 분리한 식이섬유의 포도당, 담즙산, 카드뮴 투과억제에 관한 In Vitro 연구)

  • 김미경
    • Journal of Nutrition and Health
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    • v.30 no.2
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    • pp.210-219
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    • 1997
  • Retarding effects of the dietary fibers from tangerine peels on glucose, bile acid and cadmium transport were evaluated by dialysis method, and were compared with those of commercial dietary fibers(citrus pection, CM-cellulose, guar gum, $\alpha$-cellulose). Yields of total (TDF), insoluble(IDF) and soluble dietary fibers(SDF) from tangerine peels on the fresh matter basis were 2.84%, 1.95% and 0.39% respectively. The amount of insoluble fibers was 5.2 times higher than that of soluble fibers. Soluble fibers(guar gum, CM-cellulose, SDF, pectin) had the retarding effect on glucose transport, while IDF, TDF and $\alpha$-cellulose did not have. Guar gum showed the greatest effect, followed by CM-cellulose, SDF and pectin. Among the extracted fibers, only SDF had the effect on glucose transport retardation. Regarding bile acid dialysis, guar gum had the greatest retarding effect, and all dietary fibers from tangerine peels, especially SDF, showed the effect of bile acid retardation. On cadmium transport retardation, CM-cellulose had the greatest effect, followed by SDF, TDF, IDF, guar gum and pectin. Among the extracted fibers, SDF had the greatest effect on Cd trasport transport retardation. The extracted dietary fibers showed higher retarding effect on Cd transport than glucose and bile acid transport, and the effect of SDF was higher than IDF.

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Preparation of Insoluble Dietary Fiber from Forest Waste and Its Physiological Function in Rat Fed High Cholesterol Diets

  • Chai, Young-Mi;Lim, Bu-Kug;Lee, Jong-Yoon;Rhee, Soon-Jae
    • Preventive Nutrition and Food Science
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    • v.7 no.1
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    • pp.78-87
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    • 2002
  • This study investigated the production of insoluble dietary fiber using forest waste and the dietary effect of manufactured insoluble fiber on physiological function in rat fed high cholesterol duets. Insoluble dietary fiber was prepared from the wood chips of oak (Quercus mongolica). The best condition for steam-explosion treatment for the preparation of insonuble dietary fiber was 25 kg/cm$^3$pressure for 6 minutes. In the chemical analysis of insoluble dietary fiber pretreated by 1% sodium hydroxide solution with steam-exploded wood, $\alpha$-cellulose content was 61.7% in the insoluble dietary fiber which contained 7.6% residual lignin. In order to compare insoluble dietary fiber with commercial $\alpha$-cellulose of physiological function, Sprague-Dawley male rats weighing 100$\pm$10 g were randomly assigned to one normal diet and five high cholesterol diet containing 1% cholesterol. The high cholesterol diet groups were classified as fiber free diet (FF group), 5% commercial $\alpha$-cellulose diet (5C group), 10% commercial $\alpha$-cellulose (l0C group), 5% insoluble dietary fiber dict (5M group), and 10% insoluble dietary fiber (10M group). The rats were fed ad libidum for 4 weeks. Food intake, weights gain, and food efficiency ratio in high cholesterol groups were higher than those of normal group, but there were no significant differences between the experimental groups. There were not any significant differences in the weights of livers, kidneys and small intestine of insoluble dietary fiber supplemented groups, but weight of cecum in all insolube dietary fiber group were significantly higher than those of FF group. A gstrointestinal transit time was decreased by supplementation of insoluble dietary fiber. Weight and water contents of feces in the insoluble dietary fiber supplemented groups were significantly higher than those of the FF group. There were not any significant differences in the activities of the glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT) between the experimental groups. In conclusion, the manufactured insoluble dietary fiber and commercial insoluble fiber have the same physiological effects. The preparation method of the insoluble dietary fiber from the oak chips suited its purpose.

In Vitro and In Vivo Physiological Characteristics of Dietary Fiber from By-product of Aloe vera Gel Processing (알로에 베라 유래 식이섬유의 In Vitro 및 In Vivo 생리기능 특성)

  • Baek, Jin-Hong;Cha, Tae-Yang;Heo, Jin-Chul;Lee, Sang-Han;Lee, Shin-Young
    • Food Engineering Progress
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    • v.14 no.2
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    • pp.173-182
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    • 2010
  • A fiber fraction (Aloe cellulose), the by-product obtained from Aloe vera gel processing was freeze dried and investigated for in vitro glucose/ bile acid retarding effects of powdered sample (100 mesh) comparing with commercial $\alpha$-cellulose as a reference sample. We also examined the effectiveness of physiological functionality such as the antiobesity and anti-constipation on Sprague-Dawley (SD) rat. The Aloe cellulose powders during in vitro dialysis experiment for 2 hours exhibited the glucose and bile acid retarding index of 20.32-35.2% and 53.13-28.30%, respectively. Especially, freeze dried aloe cellulose showed the 2.5 and 1.2-6 times higher effect on in vitro glucose and bile acid retardation than those of $\alpha$-cellulose. These relatively good retarding effects on glucose and bile acid diffusion suggest a potential of preventing from diabetes and arteriosclerosis of some extent. Also, the results from animal experiments on SD rats fed a high-fat diet for 4 weeks suggested that Aloe cellulose might be used as a novel dietary fiber showing an effective anti-obesity and anti-constipation effect.

The Comparison of Absorption Characteristics between High Absorbent Polymers and Cellulose (고흡수성(高吸水性) Polymer와 Cellulose의 흡수특성(吸水特性) 비교(比較))

  • Yang, In;Ahn, Won-Yung
    • Journal of the Korean Wood Science and Technology
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    • v.18 no.2
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    • pp.36-48
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    • 1990
  • This experiment was carried out to improve the quality of high absorbent polymer which has excellent absorptivity and water retention compared to pulp and absorption sheet, through absorption characteristics-absorptive power, water retention, absorption rate, gel strength, pH, particle size, and moisture content. - of six polymers, namely, anionic polyacrylamide, cationic polyacrylamide, polyacrylic, acid polyvinyle alcohol 500, and 1500, and a-cellulose. and to examine the possibility of substitution of amide groups for carboxyl group and/or hydroxyl group which were commercial high absorbent polymer by comparing the absorption characteristics of the polymers. Polyacrylamide has high absorptive power and water retention, but has low gel strength and poor absorption rate. The rest of polymers were similiar to ${\alpha}$-cellulose in every respect. Thus, polyacrylamides could be replaced with polyacrylic acid and polyvinyl alcohol which are presently a high absorbent polymers. In comparing the absorption characteristics and the absorptive power of the polymers-anionic polyacrylamide, cationic polyacrylamide, polyacrylic acid, polyvinyle alcohol. a-cellulose-the absorptive power was in inverse proportion to the gel strength and absorption rates, affected by the particle size and pH change.

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Purification and Characterization of Acid-stable ${\alpha}-Amylase$ of Aspergillus niger K-25 (Aspergillus niger 균주가 생산하는 내산성 아밀라제의 특성)

  • Cho, Myung-Hwan
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.145-148
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    • 1989
  • An acid-stable ${\alpha}-amylase$ produced by Aspergillus niger K-25 strain was purified by fractional precipitation with ammonium sulfate, ethacridine and acetone. The final preparation was homogeneous in cellulose acetate electrophoresis. The enzyme retained 91 % of its oringinal activity at pH 3.0, 8.7% at pH 2.4. The optimum pH of the enzyme was around pH 4. The purified-enzyme with optimum temperature of $40^{\circ}C$ was more heat-stable than the commercial product. The enzyme retained 80% of its original activity when heated to $60^{\circ}C$ for 30 minutes while the commercial amylase lost its acitivity completely within 30 minutes at $50^{\circ}C$.

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Screening Study for the Functionality of Psyllium Husk as a Dietary Fiber Material (Psyllium Husk의 식이섬유 소재로서의 기능성 탐색)

  • Lee, Shin-Young;Back, Jin-Hong
    • Journal of Industrial Technology
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    • v.25 no.B
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    • pp.229-239
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    • 2005
  • Dietary powder from Plantaginis ovatae testa was prepared by mechnical milling/grinding of the outer layer of the seed. The crystalline/surface structures of its powder (100 mesh) were examined, and several physical functionalities including, water capacity, oil holding capacity, emulsion/foam properties and physiological functionality such as in-vitro glucose and bile acid retarding effects were also investigated. Water holding capacity(WHC) of psyllium powder was $33.71{\pm}0.10g$ water retained/g solid at room temperature, whileas oil holding capacity(OHC) for soybean or rice bran oil were about 1.80g oil retained/g solid. These values of WHC and OHC were about 5.6 times higher and 2.8 times lower than those of commercial ${\alpha}$-cellulose, respectively. Changes of pH showed a small effect on WHC, but WHC increased with temperature. Emulsion capacity of 2%(w/v) psyllium was about 60% level of 0.5%(w/v) xanthan gum but emulsion stability after incubation of 24 hours showed about 1.4 times improvement of xanthan gum(0.5%,w/v). Also, psylliume(above 2%, w/v) alone had higher foam capacity than that of xanthan(1.1 times) and especially, 1 or 2% addition of psyllium improved the foam stability of protein solution(1% albumin+0.5% $CaCl_2$) by factor of 3.3 and 6.0 times, respectively. The glucose and bile acid retarding effects of psyllium powder were relatively very excellent suggesting the prevention from diabetes and arteriosclerosis. Especially, psyllium showed the 3.7 and 3.3 times higher effect on in-vitro glucose and bile acid retardation than those of commercial ${\alpha}$-cellulose, respectively.

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