• Steel and Composite Structures
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• 제20권5호
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• pp.1133-1153
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• 2016

In this article, an additive performance ratio method using structural analysis of both 2D and 3D is introduced to mitigate the complexity of work evaluating structural performances of numerous steel outrigger alternatives in multi-story buildings, especially high-rise buildings. The combined structural analysis process enables to be the design of economic, safe, and as constructional demanding structures by exploiting the advantages of steel, namely: excellent energy dissipation and ductility. First the approach decides the alternative of numerous steel outriggers by a simple 2D analysis module and then the alternative is evaluated by 3D analysis module. Initial structural analyses of outrigger types are carried out through MIDAS Gen 2D modeling, approximately, and then the results appeal structural performance and lead to decide some alternative of outrigger types. ETABS 3D modeling is used with respect to realization and evaluation of exact structural behaviors. The approach reduces computational burden in compared to existing concepts such as full 3D analysis methods. The combined 2D and 3D tools are verified by cycle and displacement tests including comprehensive nonlinear dynamic simulations. The advantages and limitations of the Additive Performance Ratio Approach are highlighted in a case study on a high rise steel-composite building, which targets at designing the optimized alternative to the existing original outrigger for lateral load resisting system.

• Structural Engineering and Mechanics
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• 제85권6호
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• pp.765-780
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• 2023

In this work, a novel combined logarithmic, secant and tangential 2D and quasi-3D refined higher order shear deformation theory is proposed to examine the buckling analysis of simply supported uniform functionally graded plates under uniaxial and biaxial loading. The proposed formulations contain a reduced number of variables compared to others similar solutions. The combined function employed in this study ensures automatically the zero-transverse shear stresses at the free surfaces of the structure. Various models of the material distributions are considered (linear, quadratic, cubic inverse quadratic and power-law). The differentials stability equations are derived via virtual work principle with including the stretching effect. The Navier's approach is applied to solve the governing equations which satisfying the boundary conditions. Several comparative and parametric studies are performed to illustrates the validity and efficacity of the proposed model and the various factors influencing the critical buckling load of thick FG plate.

• KIEE International Transaction on Electrical Machinery and Energy Conversion Systems
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• 제5B권3호
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• pp.224-228
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• 2005

In this paper, we investigate the main components of stray load loss of induction motors for ammonia compressors. The variations of the losses at each part of the motor due to load are calculated by the combined 3-D-2D finite element method formulated by the mixed moving coordinate systems. The stray load loss is calculated from these results due the definition of IEEE standard-112. It is clarified that the core loss and the eddy current loss of the can increase due to load, which can be considered as the main part of the stray load loss.

• BMB Reports
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• 제37권3호
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• pp.298-303
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• 2004

In general, a SYPRO Ruby dye is well known as a sensitive fluorescence-based method for detecting proteins by one-or two-dimensional SDS-PAGE (1-DE or 2-DE). Based on the SYPRO Ruby dye system, the combined two-dimensional fibrin zymography (2-D FZ) with SYPRO Ruby staining was newly developed to identify the Bacillus sp. proteases. Namely, complex protein mixtures from Bacillus sp. DJ-4, which were screened from Doen-Jang (Korean traditional fermented food), showed activity on the zymogram gel. The gel spots on the SYPRO Ruby gel, which corresponded to the active spots showing on the 2-D FZ gel, were analyzed by a matrix-assisted laser desorption ionization time of flight (MALDI-TOF) mass spectrometric analysis. Five intracellular fibrinolytic enzymes of Bacillus sp. DJ-4 were detected through 2-D FZ. The gel spots on the SYPRO Ruby dye stained 2-D gel corresponding to 2-D FZ were then analyzed by MALID TOF MS. Three of the five gel spots proved to be quite similar to the ATP-dependent protease, extracellular neutral metalloprotease, and protease of Bacillus subtilis. Also, the extracellular proteases of Bacillus sp. DJ-4 employing this combined system were identified on three gels (e.g., casein, fibrin, and gelatin) and the proteolytic maps were established. This combined system of 2-D zymography and SYPRO Ruby dye should be useful for searching the specific protease from complex protein mixtures of many other sources (e.g., yeast and cancer cell lines).

• Applied Microscopy
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• 제46권2호
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• pp.71-75
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• 2016

The scanning electron microscope (SEM) offers two-dimensional (2D) micrographs of three-dimensional (3D) objects due to its inherent operating mechanisms. To overcome this limitation, other devices have been used for quantitative morphological analysis. Many efforts have been made on the applications of software-based approaches to 3D reconstruction and measurements by SEM. Based on the acquisition of two stereo images, a multi-view technique consists of two parts: (i) geometric calibration and (ii) image matching. Quantitative morphological parameters such as height and depth could be nondestructively measured by SEM combined with special software programs. It is also possible to obtain conventional surface parameters such as roughness and volume of biomedical specimens through 3D SEM surface reconstruction. There is growing evidence that conventional 2D SEM without special electron detectors can be transformed to 3D SEM for quantitative measurements in biomedical research.

• Structural Engineering and Mechanics
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• 제12권5호
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• pp.475-490
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• 2001

Remeshing strategies are formulated for r-adaptive and h/r-adaptive analysis of crack propagation. The relocation of the nodes, which typifies r-adaptivity, is a very cheap method to optimise a given discretisation since the element connectivity remains unaltered. However, the applicability is limited. To further improve the finite element mesh, a combined h/r-adaptive method is proposed in which h-adaptivity is applied whenever r-adaptivity is not capable of further improving the discretisation. Two and three-dimensional examples are presented. It is shown that the r-adaptive approach can optimise a discretisation at minimal computational costs. Further, the combined h/r-adaptive approach improves the performance of a fully r-adaptive technique while the number of h-remeshings is reduced compared to a fully h-adaptive technique.

• Journal of Dental Anesthesia and Pain Medicine
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• 제22권2호
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• pp.87-96
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• 2022

This study aimed to assess the combined use of extraoral vibratory stimulation and extraoral cooling in reducing the pain (subjective and objective) of dental local anesthesia administration in children. PubMed, Cochrane Central Register of Controlled Trials, and Ovid SP databases were searched up to July 2021. Article titles were screened and full-text evaluations of the selected articles were performed. Finally, seven studies (391 children, aged 4 - 12 years) were included in this qualitative and quantitative analysis. The pooled data determined the combined effect of extraoral vibration and extraoral cooling as a single measure. Extraoral vibration or cooling alone were not compared. The measured primary and secondary outcomes were pain perception and subjective and objective pain, respectively. When compared with the control, extraoral vibration and cooling resulted in significant differences in the mean combined data for the variables, pain perception, and pain reaction. Children's subjective pain as measured by pain scores were reduced when extraoral vibration and cooling was used during local anesthesia administration (mean difference -3.52; 95% confidence interval [-5.06 - 1.98]) and objective pain (mean difference -1.46; 95% confidence interval [-2.95 - 0.02] ; mean difference -1.93; 95% confidence interval [-3.72 - 0.14]). Within the confines of this systematic review, there is low-quality evidence to support the use of combined extraoral vibration and cooling for reducing pain (subjective and objective) during intraoral local anesthesia administration in children.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7857-7861
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• 2014

Natural killer (NK) cells play an important role in anti-tumor immunity. Interleukin (IL)-18 is an immunoregulatory cytokine that induces potent NK cell-dependent anti-tumor responses when administrated with other cytokines. In this study, we explored the effects of combining IL-18 and IL-2 on NK cytotoxicity as well as expression levels of the NK cell receptor NKG2D in vitro. Freshly isolated PBMCs were incubated for 48 h with IL-18 and IL-2, then CD107a expression on $CD3^-CD56^+$ NK cells was determined by three-colour flow cytometry to evaluate the cytotoxicity of NK cells against human erythroleukemia K562 cells and human colon carcinoma HT29 cells. Flow cytometric analysis was also employed to determine NKG2D expression on NK cells. The combined use of IL-18 and IL-2 significantly increased CD107a expression on NK cells compared with using IL-18 or IL-2 alone, suggesting that the combination of these two cytokines exerted synergistic enhancement of NK cytotoxicity. IL-18 also enhanced NKG2D expression on NK cells when administered with IL-2. In addition, blockade of NKG2D signaling with NKG2D-blocking antibody attenuated the up-regulatory effect of combining IL-18 and IL-2 on NK cytolysis. Our data revealed that IL-18 synergized with IL-2 to dramatically enhance the cytolytic activity of human NK cells in a NKG2D-dependent manner. The results appear encouraging for the use of combined IL-18 and IL-2 in tumor immunotherapy.

• Structural Engineering and Mechanics
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• 제5권6호
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• pp.743-754
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• 1997

A three-dimensional constitutive modeling for reinforced concrete is presented for finite element nonlinear analysis of reinforced concrete. The targets of interest to the authors are columns confined by lateral steel hoops, RC thin shells subjected to combined in-plane and out-of-plane actions and massive structures of three-dimensional (3D) extent in shear. The elasto-plastic and continuum fracture law is applied to pre-cracked solid concrete. For post cracking formulation, fixed multi-directional smeared crack model is adopted for RC domains of 3D geometry subjected to monotonic and reversed cyclic actions. The authors propose a new scheme of decomposing stress strain fields into sub-planes on which 2D constitutive laws can be applied. The proposed model for 3D reinforced concrete is experimentally verified in both member and structural levels under cyclic actions.

• Journal of Plant Biology
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• 제39권4호
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• pp.301-307
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• 1996

The disassembly of Chl-protein complexes during dark-induced senescence (DIS) was investigated using detached third and fourthleaves of 21$\pm$1 day-old Arabidopsis thaliana. Although Chl content decreased linearly after 1 d, a significant decrease of photochemical effeciency (Fv/Fm) was observed after 2 d. In experiments using native green gel electrophoresis of Chl-protein complexes combined with additional two-dimensional SDS-PAGE analysis, we could observe the degradation of both photosystems after 2 d. Although light-harvesting complex(LHC) for PSI (LHCI) was degraded first in PSI complex, small PSII apoproteins including CP47/CP43 and D1/D2 apoproteins were degraded first in PSII complexes. LHC for PSII (LHCII) trimers were stable until 4 d. The level of LHCII monomers was increased until 3 and decreased thereafter, resulting in the increase of free pigments. These results suggest that the disassembly process of PSI is different from that of PSII.