• 한국동물위생학회지
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• 제31권1호
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• pp.1-16
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• 2008

Salmonella infections cause the disease in pigs but also some zoonotic Salmonella serotypes can be transmitted to human through swine products, resulting in food poisoning. The objective of this study was to investigate the bacteriological prevalence and detection of invA gene using Salmonella specific polymerase chain reaction (PCR), the epidemiological characteristics related to Salmonella strains cultured from pig samples in Gangwon areas using serotyping, random amplified polymorphic DNA (RAPD) and pulsed field gel electrophoresis (PFGE) methods. During the period of November 2001 through April 2002, 1,174 ileocecal lymph node were collected from the slaughtered pigs raised in 38 farms located in Gangwon province. The samples were submerged in boiling water and macerated in saline and lymph node homogenates were inoculated into Tetrathionate broth with iodine (TTB, Difco, 0.5% iodine was added) for enrichment growth. Then additional tests were performed using several mediums, and suspects were identified by API 20E kit (BioMerieux) and PCR. Of total 1,174 samples from 38 farms, 44 (3.7%) were isolated as Salmonella spp from 13 farms (34.2%). Of 44 isolates, 31 were in Yangyang region, followed by 9 in Goseong, 2 in both Gangneung and Sokcho. However, there was no difference in regional isolation frequency. All isolates have a 521bp amplified product in Salmonella specific PCR with primer invA which encodes in proteins for invasion of epithelial cells. Of 44 recovered serotypes, 23 (52.3%) were S Eingedi, 10 (22.7%) S Schwarzengrund, 9 (20.5%) S Typhimurium, and 2 (4.5%) S Mbandaka. In RAPD analysis, there appeared to be unique bands distinguishing each serotype, although similarities exist between the different serotypes. Four serotypes of 44 Salmonella isolates appeared to fall into 14 different RAPD types. In PFGE analysis, 9 S Typhimurium were tested with XbaI enzyme and SpeI enzyme. The combination of results obtained with two enzymes subdivided the 9 S Typhimurium into 4 PFGE types.

• 한국기계가공학회지
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• 제10권6호
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• pp.50-55
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• 2011

The fluid power products are widely used in current industrial area such as automation of products and equipment assembly, high-tech machine tool, aircraft, train, and etc. As the development of industry is in progress, the development of the fluid power products is demanding and it is required in every industrial area. This research proposed a pneumatic system to evaluate displacement accuracy of the pneumatic actuator without external load and to analyze capability of integration of the valve system. The pneumatic system consisted of a combination of pneumatic actuator, four two-port valves, two three-port valves, two pressure valve, a check valve, two proximity sensors, and a program logic controller (PLC). The position controller is based on the PLC connected with the proximity sensors. The maximum air pressure applied for tests was $49.05N/cm^2$ and the displacement accuracy of a stroke was measured using a dial gauge. The supply- and discharge-side of air pressure and the length of the stroke of the pneumatic cylinder were varied The test of the position control of the pneumatic cylinder was carried out 50 times at each supply- and discharge-side air pressure of 24.53/34.34, 29.43/39.24, 34.34/44.15, and $39.24/49.05N/cm^2$ and replicated three times. The accuracy of the displacement of the pneumatic cylinder stroke increased as the supply- and discharge-side of air pressure increased with the stroke length of 133mm. Also the displacement accuracy increased as the stroke length increased with the fixed supply- and discharge-side of air pressure of the pneumatic cylinder as 34.34 and $44.15N/cm^2$, respectively. The most accurate displacement of the pneumatic cylinder was obtained at the supplyand discharge-side of air pressure of 39.24 and $49.05N/cm^2$, respectively, and strokes of 170 and 190mm.

• 자동차안전학회지
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• 제7권3호
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• pp.7-13
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• 2015

Occupant protection performance in frontal crashes has been developed and assessed for mainly front seat occupants over many years, and in recent years protection of rear seat occupants has also been extensively discussed. Unlike the front seats, the rear seats are often occupied by children seated in rear-facing or forward - facing child restraint systems, or booster seats. In the ENCAP, child occupant protection assessments using 18-month-old(P1.5) and 3-year-old(P3) test dummies in the rear seat have already been changed to new type of 18-month-old (Q1.5)and 3-year-old(Q3) test dummies. In addition, ENCAP are scheduled with the development and introduction of test dummies of 6-year-old (Q6) and 10.5-year-old children(Q10) starting 2016. In KNCAP, Q6 and Q10 child dummies will be introduced in 2017 as well. Automobile manufacturers need to develop safety performance for new child dummies closely. In this paper, we focused on Q6 and Q10 child dummies sitting in child restraint system. Offset frontal crash tests were conducted using two types of test dummies, Q6 and Q10 child dummies, positioned in the rear seat. Q6 and Q10 were used to compare dummy kinematics in rear seating positions between Q6 behind the driver's seat and Q10 behind the front passenger's seat. The full vehicle sled test results of both dummies were conducted with different restraint systems. It showed that several injury and image data was collected as the result of the full vehicle sled test. Based on the results of these investigations, this paper describes which factor is most important and combination is the best performance when evaluating rear seat occupant protection for Q6 and Q10 child dummies.

• Journal of Preventive Medicine and Public Health
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• 제40권4호
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• pp.285-290
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• 2007

Objectives : Cases of human brucellosis in Korea have recently increased due to the increasing incidence of bovine brucellosis. The authors conducted this study to elucidate the status of brucellosis through seroepidemiologic study. Methods : We selected our study population from a high risk group. We conducted a questionnaire survey and obtained blood samples to determine the seroprevalence of brucellosis antibodies for 10 days in February, 2005. The titers of brucellosis were measured by the combination of standard tube agglutination test (STA) and enzyme-linked immunosorbent assay (ELISA) test. Results : Our study subjects comprised 1,075 cases: 971 livestock workers, 51 veterinarians, and 53 artificial inseminators. In the STA test, 27 cases (2.5%) had titers of greater than or equal to 1:20. Of 1,068 cases (7 cases were excluded due to previous brucellosis), 7 cases of brucellosis were diagnosed with titers of 1:160, giving a seroprevalence of brucellosis of 0.66%. The seroprevalence in the male group was 0.95%, and that of livestock workers, veterinarians, and artificial inseminators was 0.52%, 4.17%, and 0.00%, respectively. The Spearman's correlation coefficient between the positive rate of bovine brucellosis per capita and household and human brucellosis was 0.806 and 0.744, respectively. The concordance rate between the Korea National Institute of Health and the Gyeongsangbuk-do Institute of Health and Environment by the STA and ELISA tests was 94.7% and 100.0%, respectively. Conclusions : The study results indicated in higher seroprevalence rate among veterinarians than among livestock workers and artificial inseminators. Because veterinarians may be exposed to this high risk, effective working guidelines for veterinarians to guard against brucellosis must be developed. Moreover, more extensive epidemiologic research for laboratory workers and meat handlers is needed.

• 한국지반공학회논문집
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• 제27권1호
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• pp.17-24
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• 2011

CLSM의 플로우 및 강도특성은 비회, 매립회, 시멘트, 수량 등과 같은 배합비에 크게 의존하므로, 각 구성요소들의 배합비와 플로우 및 강도값에 대한 역학적 관계를 정량적으로 도출하기가 현실적으로 매우 어렵다. 따라서 CLSM의 구성성분 비율에 대한 플로우 및 압축강도값을 도출할 수 있는 산정방법이 필요하다. 이에 본 연구에서는 인공신경망 학습을 통해 플로우 및 일축압축강도를 실험을 통하지 않고 인공신경망을 이용하여 CLSM의 플로우 및 일축압축강도를 예측하고자 한다. 본 연구에 사용한 인공신경망모델에는 BPNN 학습 알고리즘을 적용, 인공신경망 학습효율 및 예측능력에 영향을 미치는 은닉층, 모멘텀상수, 목표시스템 오차값, 은닉층의 노드 수와 학습률을 변화시키면서 학습하여 각각의 변화에 따른 인공신경망 모델의 학습효율 및 예측능력을 평가하고 인공신경망의 유효성 검증을 위해 모델 구축 시에 사용하지 않은 새로운 자료에 대해 예측을 실시하여 실내실험 결과와 비교하여 이를 기준으로 CLSM의 플로우 및 압축강도 산정에 적합한 최적인공신경망 모델을 제안하였다.

• 한국지반공학회논문집
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• 제32권9호
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• pp.17-27
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• 2016

서해안의 인천 및 화성지역에 분포하는 모래 및 실트 함유량이 많은 저소성 지반에 대해 피에조콘관입시험(CPTU) 데이터 및 강제치환 공법을 이용하여 부분배수 특성을 분석하였다. Powell과 Quarterman(1988)에 의한 과압밀비 $OCR={\kappa}(q_t-{\sigma}_{vo})/{\sigma}^{\prime}_{vo})$ 경험식은 모래함유량이 많은 서해안 저소성 실트 지반에서는 상대적으로 투수성이 커서 표준관입속도(2cm/s)하에서 콘관입저항력($q_t$)이 크게 평가되어 과압밀비가 크게 산정되는 경향을 나타냈다. Schnaid et al. (2004)는 간극수압계수($B_q$)-강도증가율($s_u/{\sigma}^{\prime}_{vo}$)-정규화된 콘저항($Q_t=(q_t-{\sigma}_{vo})/{\sigma}^{\prime}_{vo}$)을 함께 도시하여, 부분배수 유무를 판단하도록 제시하였는데, 인천 및 화성 지역의 CPTU 데이터의 50% 이상이 부분배수 상태를 나타내는 $B_q$ < 0.3에 분포하였다. 또한, 강제치환 시공과정 중 부분배수 현상으로 인해 원지반의 강도증가 현상이 발생되어 설계 예상 치환깊이보다 훨씬 작은 실측값이 얻어진다는 관점에서 실측 치환깊이와 동일한 값이 얻어지도록 원지반의 지지력에 대한 역해석을 수행하였다. 그 결과, 소성지수가 감소할수록 내부마찰각이 커지는 경향을 나타내며, 내부마찰각(${\varphi}^{\prime}$)이 $2{\sim}7^{\circ}$의 범위에서 분포하는 것으로 분석되었다.

• 콘크리트학회논문집
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• 제13권2호
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• pp.99-106
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• 2001

이 연구의 목적은 기존의 단순지지로 더블티 슬래브에 발생하는 과도한 모멘트, 슬래브 깊이, 중앙부 처짐, 단부 균열의 발생 원인을 근원적으로 해결할 연속화된 개량조인트를 개발하는데 그 초점을 두었다. 개량조인트는 다음과 같은 장점을 얻을 수 있다. 먼저, 보중앙 정모멘트의 감소로 인하여 적용하중에 대한 설계스팬을 증가시킬 수 있으며, 슬래브의 처짐을 감소시킬 수 있다. 또한, 기존 더블티공법의 문제점인 더블티와 역티사이의 연결부 균열을 감소시킬 수 있으며, 시공시 부가적인 거푸집을 필요로 하지 않는다. 개선된 더블티의 댑단부와 직사각형보를 연결부 시험체를 활용하여, 길이철근 배근량을 변수로한 정적 3점재하 실험에서 전단력과 모멘트에 대한 거동을 평가하였다. 이 연구의 범위 안에서 다음과 같은 결론을 얻을 수 있다. 개량댑에 의한 연결 부의 연속화는 토핑 콘크리트에 길이 방향 철근을 배근함으로써 효과적으로 구할 수 있으며, 더블티 슬래브의 스팬용량을 증 가시키고 처짐을 일반적으로 감소시킨다. 아울러, 개량댑의 연속화는 단순지지 연결부보다 초기균열에 효과적인 것으로 나타났다.

• 한국산학기술학회논문지
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• 제12권4호
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• pp.1786-1795
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• 2011

사람이 생활하는 환경에서 일반적인 휠베이스 이동(Mobility) 방식의 로봇은 장애물에 둘러싸여 로봇의 움직임에 있어 자유로운 주행 제약을 받게 된다. 장애물을 신속하게 회피하려면 회전과정 없이 단순히 좌우 이동만 하면 되는 홀로노믹(Holonomic) 시스템 특성의 이동로봇이 필요하다. 본 논문에서는 세 개의 옴니휠(Omni-Wheels)을 사용한 홀로노믹 이동로봇의 추적제어기를 개발한다. 옴니휠을 이용한 이동로봇은 시스템 파라미터의 불확실성(uncertainty)으로 인하여 선형 제어기로는 추적제어가 매우 어려운 상황이다. 그러므로 강인성이 탁월한 퍼지 제어기를 이용한 퍼지 적응 제어 기법을 설계하여 옴니휠 이동 로봇의 추적제어(tracking control) 성능을 높인다. 본 논문에서 제어 대상 시스템의 매개 변수의 불확실성에 강인한 퍼지 제어기를 병렬로 설계하고 시스템 인식(system identification)을 이용하여 대상 시스템이 특성 변화에 적절히 대처할 수 있는 적합한 퍼지 제어기를 선택한 후 피드백 제어를 실행하는 퍼지 다층 제어기(Fuzzy Multi-Layered Controller) 시스템을 이용한 적응 제어기법을 제시한다. 고전 적응 제어기와 기존 퍼지 적응 제어기의 문제점을 극복한 퍼지 적응 제어기를 도입하여 강인 제어기를 병렬로 설계하고 시스템 인식을 이용하여 대상 시스템의 특성 변화에 적절히 대처할 수 있는 적합한 퍼지 제어기를 선택한 후 피드백 제어를 실행하는 퍼지 다층 제어기(FMLC)를 제시한다.

• 한국전통의학지
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• 제15권1호
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• pp.113-127
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• 2006

Objectives: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased nitric oxide(NO) and inducible NO synthase (iNOS) generation in cellular experiments. Methods: Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. Diluted moxi-tar was single injected in several acupoints or combined with electroacupuncture (l ms, 2 Hz, and 2 mA) into contralateral ST36 acupoint for 30 min to assess the synergic effects. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord were examined on a rat model of carrageenan-induced arthritis. Results : After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Maxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Maxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by maxi-tar. Moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that maxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• Annals of Surgical Treatment and Research
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• 제95권6호
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• pp.303-311
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• 2018

Purpose: OncoHepa test is a multigene expression profile test developed for assessment of hepatocellular carcinoma (HCC) prognosis. Multiplication of ${\alpha}$-FP, des-${\gamma}$-carboxy prothrombin (DCP) and tumor volume (TV) gives the ${\alpha}$-FP-DCP-volume (ADV) score, which is also developed for assessment of HCC prognosis. Methods: The predictive powers of OncoHepa test and ADV score were validated in 35 patients who underwent curative hepatic resection for naïve solitary HCCs ${\leq}5cm$. Results: Median tumor diameter was 3.0 cm. Tumor recurrence and patient survival rates were 28.6% and 100% at 1 year, 48.6% and 82.9% at 3 years, and 54.3% and 71.4% at 5 years, respectively. The site of first tumor recurrence was the remnant liver in 18, lung in 1, and the peritoneum in 1. All patients with HCC recurrence received locoregional treatment. OncoHepa test showed marginal prognostic significance for tumor recurrence and patient survival. ADV score at 4log also showed marginal prognostic difference with respect to tumor recurrence and patient survival. Combination of these 2 tests resulted in greater prognostic significance for both tumor recurrence (P = 0.046) and patient survival (P = 0.048). Conclusion: Both OncoHepa test and ADV score have considerably strong prognostic power, thus individual and combined findings of OncoHepa test and ADV score will be helpful to guide postresection surveillance in patients with solitary HCCs ${\leq}5cm$.