• Korean Journal of Pharmacognosy
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• v.47 no.1
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• pp.12-17
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• 2016

In this study, the purification of the MeOH extract from the stems and leaves of Pourthiaea villosa var. brunnea using column chromatography furnished a main compound, lyoniside. The structure was elucidated on the basis of $^1H$ and $^{13}C$ NMR spectroscopic data. Quantitative analysis of lyoniside was conducted by HPLC method and the highest content of lyoniside was found in 50% MeOH reflux extraction. To investigate the anti-inflammatory effect of the lyoniside, we measured nitric oxide and pro-inflammatory cytokines such as TNF-${\alpha}$, IL-$1{\beta}$, IL-6 levels in lipopolysaccharide-induced murine macrophage cell line RAW 264.7. As a results, lyoniside decreased the level of nitric oxide and IL-6 in concentration dose dependent manner in RAW 264.7 cells.

• Korean Journal of Pharmacognosy
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• v.47 no.3
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• pp.211-216
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• 2016

In the course of our continuing search for biologically active components from Korean medicinal plants, we isolated the main compound, luteolin 5-glucoside from aqueous fraction of Ajuga spectabilis. The structure was elucidated by the basis of $^1H$ and $^{13}C$ NMR and TOF ESI-MS data. Quantitative analysis of luteolin 5-glucoside was carried out on a XBridge C18 column ($S-5{\mu}m$, $4.6{\times}250mm$) with gradient elution composed of acetonitrile:water. The results exhibit that the average content of main compound in A. spectabilis were 0.048%. Oxidative stress plays a major role Alzheimer's disease (AD) and other neurodogenerative disease. AD is major health problem and there is currently no clinically accepted treatment to cure or stop its progression. Pretreatment with luteolin 5-glucoside markedly attenuated $H_2O_2$-induced cell viability loss in a dose-dependent manner. Luteolin 5-glucoside also inhibited the formation of intracellular reactive oxygen species in SH-SY5Y. The results suggest that luteolin 5-glucoside from A. spectabilis has protective effects against oxidative stress-induced cytotoxicity, which might be a potential therapeutic compound for treating and/or preventing neurodegenerative disease implicated with oxidative stress.

• Journal of Korean Society of Steel Construction
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• v.16 no.5 s.72
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• pp.629-639
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• 2004

A steel frame is one of the most commonly used structural systems due to its resistance to various types of applied loads. Many studies have been conducted to investigate the effects of several parameters, such as connection flexibility, the boundary condition of each support, and beam-to-column stiffness ratio, on the characteristic behavior of a frame. Based on the results of these studies, several design methods have been proposed. This research focused on the number of bolts on the rotational stiffness of a double-angle connection, and its effect on the story drift of a frame. To achieve these purposes, a simplified analytical model was proposed. Several experimental tests were also conducted to obtain the rotational connection stiffness of each double-angle connection.

• 한국해양학회지
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• v.24 no.3
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• pp.121-131
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• 1989

Cold water observed at sea surface near the southeastern coast of Korea in summers 1982 and 1983 was studied by using data of hydrography, sea level, wind and satellite image. In summer season when water column shows 3-layered structure a "full" upwelling occurs by southwesterly transient wind continuing for several days. During upwelling event, surface water of high temperature moved offshore, middle water of low temperature outcropped to the sea surface, and sea level was lowered, however, equilibrium depth of surface layer was not changed. It may be concluded that cold water at the surface originates from middle layer and strong surface front is a result of surfacing of seasonal thermocline. In order to see the relationship between position of surface front and wind input, a model of Csanady (1982) was applied in a rigid lid approximation. The results show that frontal position can be determined by wind input and water structure near the southeastern coast of Korea. Cold water in summer can appear at the sea surface only when there is wind larger than a minimum wind impulse of order $10m^2/sec$.

• Journal of Soil and Groundwater Environment
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• v.20 no.4
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• pp.41-50
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• 2015

Open-loop groundwater heat pump (GWHP) system generally has benefits such as a higher coefficient of performance (COP), lower initial cost, and flexible system size. The hydrogeological conditions in Korea have the potential to facilitate the use of the GWHP system because a large number of monitoring wells show stable groundwater temperatures, shallow water levels, and high well yields. However, few studies have been performed in Korea regarding the GWHP system and the most studies among them dealt with Standing Column Well (SCW). Because the properties of the aquifer have an influence on designing open-loop systems, it is necessary to perform studies on various hydrogeological settings. In this study, the hydrogeological and thermal properties were estimated through various tests in the riverside alluvial layer where a GWHP system was installed. Under different groundwater flow velocities and pumping and injection rates, a sensitivity analysis was performed to evaluate the effect of such properties on the design of open-loop systems. The results showed that hydraulic conductivity and thermal dispersivity of the aquifer are the most sensitive parameters in terms of performance and environmental aspects, and sensitivities of the properties depend on conditions.

• Analytical Science and Technology
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• v.10 no.6
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• pp.408-417
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• 1997

Normal phase or reversed phase liquid chromatographic separation of some structural isomers of functionalized heterocyclic compounds has been carried out by using several different columns and various mobile phases. The optimal experimental conditions for separation of structural isomers were found on a ternary solvent system including alcohol as a modifier. This polar modifier is preferentially adsorbed onto strong adsorption site, leaving a more uniform population of weaker site that then serve to retain the sample. This 'deactivation' of the adsorbent leads to a number of improvements in subsequent separations. The optimal mobile phase system of separation were found on normal phase on structural isomers. Retention mechanism of normal phase system was also studied depending on adsorption strength between solute and stationary phase of column. However, retention factors of reversed phase system were found on hydrophobic interaction with solvophobic effect.

• Applied Chemistry for Engineering
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• v.18 no.6
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• pp.537-544
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• 2007

This study was carried out to investigate adsorption characteristics of residual pesticides on an adsorbent. $Bauxsol^{TM}$ and mackban-stone as adsorbent were used. Analytical method for residual pesticides was established by GC/NPD and $GC/{\mu}ECD$. Pesticides used in this study were ${\alpha}$-endosulfan, ${\beta}$-endosulfan, pendimethalin and chlorpyrifos-methyl, fenitrothion, and recovery rates were more than 97%. Adsorption rate on an adsorbent was decreased in order of endosulfan, chlorpyrifos-methyl, pendimethalin, fenitrothion. Organochlorine pesticides showed higher removal rate than the other pesticides. $Bauxsol^{TM}$ gave both chemical decomposition and physical adsorption. Mackban-stone gave only physical adsorption on the other hand. The high pH and chloric ions structure of eluted solution have greatly affected at chemical resolution. The removal rate of pesticides was increased due to the physical property of adsorbent, i.e. high porosity. The above adsorbent is to be a candidate to remove residual pesticides in water and pond of links.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.1
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• pp.32-39
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• 2018

Repairing timing and the extended service life with repairing are very important for cost estimation during operation. Conventionally used model for repair cost shows a step-shaped cost elevation without consideration of variability of extended service life due to repairing. In the work, RC(Reinforced Concrete) Column is considered for probabilistic evaluation of repairing number and cost. Two mix proportions are prepared and chloride behavior is evaluated with quantitative exterior conditions. The repairing frequency and cost are investigated with varying service life and the extended service life with repairing which were derived from the chloride behavior analysis. The effect of COV(Coefficient of Variation) on repairing frequency is small but the 1st repairing timing is shown to be major parameter. The probabilistic model for repairing cost is capable of reducing the number of repairing with changing the intended service life unlike deterministic model of repairing cost since it can provide continuous repair cost with time.

• Journal of Microbiology and Biotechnology
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• v.15 no.2
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• pp.330-336
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• 2005

Lactococcus lactis A164 is a nisin Z-producing strain isolated from kimchi. Its antimicrobial spectrum has been found to be active against most Gram-positive bacteria tested, yet inactive against Gram-negative bacteria [3]. Accordingly, to overcome this drawback, the current study attempted to express human $\beta$-defensin-l (hBD-l), which kills both Gram-positive and Gram-negative bacteria in L. lactis AI64. When the hBD-l cDNA was introduced using a nisin Z-controlled expression cassette, the L. lactis A164 transformants grew very poorly, due to the bactericidal effect of the expressed hBD-l against the transformants. Therefore, a gene fusion system was designed to reduce the toxicity of the expressed heterologous protein against the host cells. As such, the hBD-l gene was fused to the DsbC- Tag of pET -40b(+), then introduced to L. lactis A 164. The transformants expressed an intracellular 35.6-kDa DsbC-hBD-l fusion protein that exhibited slight activity against the host cells, yet not enough to strongly inhibit the cell growth. To obtain the recombinant hBD-l, the DsbC-hBD-l fusion protein was purified by nickel-affinity column chromatography, and the DsbC-Tag removed by cleaving with enterokinase. The cleaved mature hBD-l exhibited strong bactericidal activity against E. coli JM109, indicating that the recombinant L. lactis A 164 produced a biologically active hBD-I. In addition, the recombinant L. lactis A 164 was also found to produce the same level of nisin Z as the wild-type.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.22 no.1
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• pp.34-46
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• 2010

We observed tidal currents throughout all four seasons in 2007 at a single station, located 1.6km off Haeundae Beach and compared these current data with wind data. The direction of seasonal wind represented a similarity between the winds at sea and on land but the speed of wind at sea was almost three times stronger than the wind on land. In addition, the wind at sea turned out to considerably affect on tidal currents, particularly from late summer to autumn. On the other hand, the thickness of Ekman Layer, indicating a limitation of wind influence, was estimated to be 31.8 m on average, suggesting that the entire water column is under the influence of wind. Therefore, we are required to consider the wind stress into the analysis of tidal currents for the prevention of the loss of sand from Haeundae Beach.