• Current Research on Agriculture and Life Sciences
• /
• 제19권
• /
• pp.39-43
• /
• 2001

본 연구는 꿀벌의 월통에 얄맞은 실내 온도인 $2{\sim}9^{\circ}C$를 유지하면서 환기를 계속할 수 있는 저온양봉사에서 꿀벌의 실내월동 방법을 확립코자 수행되었다. 환기 팬의 작동 시간으로 실내 온도가 $2{\sim}9^{\circ}C$로 조절되고 환기가 행하여지는 저온양봉사에서 서양종 봉군을 공시하여 1997년부터 1999년까지 실내월동 시험을 수행한 성적은 다음과 같다. 실내월동 봉군의 폐사율은 1997~1998년 월동기간 중에 6.3~7.1% 그리고 1998~1999년 월동기간 중에 5~10%였다. 실내월동 시 월동 기간 중 봉군의 무게 감량 비율은 강군이 10.6~10.7% 그리고 약군이 10.2~11.7%였다. 월동 후 봉세 증가율은 강군의 경우는 136.1~142.3%였고, 약군의 경우는 128.0~136.5%였다.

• 한국수산과학회지
• /
• 제54권5호
• /
• pp.751-760
• /
• 2021

This study investigated the changes in water quality parameters and microbial colonies when ozone was applied to a semi-recirculating aquaculture system (semi-RAS) for the olive flounder Paralichthys olivaceus (500 g in average weight). Concentrations of ozone-produced oxidants (OPO) in rearing tanks were maintained at 0, 0.014, 0.025 mg/L as Cl₂ for 26 days. Except total ammonia nitrogen, nitrite nitrogen, nitrate nitrogen, phosphate phosphorus, chemical oxygen demand, and total suspended solids decreased significantly with increasing OPO concentration in daily and weekly monitoring (P<0.05). Colony forming unit (CFU) counts of heterotrophic marine bacteria decreased in an OPO concentration-dependent manner. Overall reduction rates of microbial colonies in the treatments were 80% higher than those of the control (P<0.05). During the experiment, the OPO concentration-driven ozonation was reliably practiced without any adverse effects on the animals cultured in semi-RAS. Considering the biohazard, operating cost, and stability of ozonation, an OPO concentration of 0.014 mg/L would be sufficient to control water quality parameters and microbial colonies in a semi-RAS.

• 생체재료학회지
• /
• 제22권4호
• /
• pp.271-278
• /
• 2018

Background: The chondrogenic differentiation of mesenchymal stem cells (MSCs) is regulated by many factors, including oxygen tensions, growth factors, and cytokines. Evidences have suggested that low oxygen tension seems to be an important regulatory factor in the proliferation and chondrogenic differentiation in various MSCs. Recent studies report that synovium-derived mesenchymal stem cells (SDSCs) are a potential source of stem cells for the repair of articular cartilage defects. But, the effect of low oxygen tension on the proliferation and chondrogenic differentiation in SDSCs has not characterized. In this study, we investigated the effects of hypoxia on proliferation and chondrogenesis in SDSCs. Method: SDSCs were isolated from patients with osteoarthritis at total knee replacement. To determine the effect of oxygen tension on proliferation and colony-forming characteristics of SDSCs, A colony-forming unit (CFU) assay and cell counting-based proliferation assay were performed under normoxic (21% oxygen) or hypoxic (5% oxygen). For in vitro chondrogenic differentiation, SDSCs were concentrated to form pellets and subjected to conditions appropriate for chondrogenic differentiation under normoxia and hypoxia, followed by the analysis for the expression of genes and proteins of chondrogenesis. qRT-PCR, histological assay, and glycosoaminoglycan assays were determined to assess chondrogenesis. Results: Low oxygen condition significantly increased proliferation and colony-forming characteristics of SDSCs compared to that of SDSCs under normoxic culture. Similar pellet size and weight were found for chondrogensis period under hypoxia and normoxia condition. The mRNA expression of types II collagen, aggrecan, and the transcription factor SOX9 was increased under hypoxia condition. Histological sections stained with Safranin-O demonstrated that hypoxic conditions had increased proteoglycan synthesis. Immunohistochemistry for types II collagen demonstrated that hypoxic culture of SDSCs increased type II collagen expression. In addition, GAG deposition was significantly higher in hypoxia compared with normoxia at 21 days of differentiation. Conclusion: These findings show that hypoxia condition has an important role in regulating the synthesis ECM matrix by SDSCs as they undergo chondrogenesis. This has important implications for cartilage tissue engineering applications of SDSCs.

• 농업과학연구
• /
• 제49권3호
• /
• pp.389-396
• /
• 2022

The purpose of this study was to investigate the safety of Houttuynia cordata Thunb extract (HCE) in Institute of Cancer Research (ICR) mice, to determine the effects of the extract on the growth performance and colony count, and to establish the optimal concentration of HCE. In total, 60 five-week-old male ICR mice with an average initial body weight (BW) of 27.24 ± 0.44 g were used in a four-week experiment. Mice were randomly allotted to four treatment groups (five replications per group, three mice per cage): 1) a control (CON) group fed with normal distilled water; 2) treatment group 1 (T1) fed with normal distilled water containing 0.05% HCE; 3) treatment group 3 (T3) fed with normal distilled water containing 0.1% HCE; and 4) treatment group 3 (T3) fed with normal distilled water containing 0.2% HCE. BW, feed intake (FI), and water intake were measured on the first, fourteenth, and eighteenth days. T2 showed a significant improvement (p < 0.05) in the feed conversion ratio (FCR) over the experimental period. However, water intake levels did not show significant differences among the groups. In the large intestine and feces, E. coli and Lactobacillus levels were significantly improved (p < 0.05) in the HCE treated group compared to the CON group. Supplying HCE via the drinking water improved the growth performance and colony count in ICR mice. Based on results of this study, utilizing HCE in livestock species is expected to be safe and feasible.

• Journal of Microbiology and Biotechnology
• /
• 제10권3호
• /
• pp.321-326
• /
• 2000

To examine the feasibility of the long-term production of the human granulocyte colony stimulating factor (hG-CSF) using the $_{L}-arabinose$ promoter system of Escherichia coli, flask relay culture and cyclic fed-batch culture were performed. In the flask relay culture, it was found that the pismid was maintained stably up to about 170 generations in an uninduced condition, whereby the cells could also maintain the capability of expressing hG-CSF expression were maintained stably up to at least 100 generations. In contrast, in the cyclid fed-batch culture, segregational plasmid instability was observed within about 4 generations after induction, even though the cell growth and hG-CSF production reached their maximum balues, 78.0 g/l of dry cell weight and 7.0 g/l of hG-CSF, respectively. It would appear that, when compared to the flask relay culture, the high-cell density and high-level expression of hG-CSF in the cyclic fed-batch cultrure led to the segregational plasmid instability; in other words, a severe metabolic burden existe on the cells due to the high-level expression of hG-CSF. Accordingly, based on these long-term cultures, the segregational and structural plasmid instability was observed and a strategy to overcome such problems could be designed.

• 동아시아식생활학회지
• /
• 제11권4호
• /
• pp.305-314
• /
• 2001

The study was conducted to investigate the effect of added green tea and black tea powder on the quality of bread during storage. Tea bread was prepared with 0.1% and 2% concentration of green tea (GT) and black tea(BT) powder. The crude fat contents of GT 2% group and BT 2% group were significantly higher than those of control group. GT l% group and BT l% group. The loaf weight of the control group was the lowest and its loaf volume was the highest. Especially, GT 2% group had the lowest loaf volume. The internal lightness value of control group was the highest. the redness value of BT 2% group and the yellowness value of GT 2% group were the highest. GT 2% group was significantly higher in hardness and gumminess and BT l% group was higher in cohesiveness and springiness. After 5 day storage, the retrogradation of GT 2% group was the highest and that of BT 2% group was the lowest among groups. After 5 day storage. pH. titratable acidity and total colony count of GT 2% group was the lowest and those of control group was the highest. The sensory score of control, GT l% and GT 2% group were evaluated higher than others. The shelf-life of tea breads were prolonged to 1~3 days by adding green tea powder and black tea powder as compared with the control group. but It must be considered the way to increase the quality of bread in loaf volume and retrogradation.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권1호
• /
• pp.12-16
• /
• 2004

Partitioning of human granulocyte-macrophage colony stimulating factor (hGM-CSF) was achieved in the aqueous two-phase systems (ATPSs) using a crude extract of transgenic tobacco cell suspension culture. This study examined the effects of polyethylene glycol (PEG) molecular weight and concentration and the effects of sodium phosphate concentration in different PEG/sodium phosphate systems on the partition coefficient, K. The best ATPS system was 5% PEG 8,000/1.6 M sodium phosphate after 2 h of incubation at room temperature. In this system, hGM-CSF was partitioned in the PEG-rich phase with a yield of 57.99% and K$\_$hGM-CSF/ of 8.12. In another system, 3% PEG 10,000/1.6 M sodium phosphate, hGM-CSF was also partitioned primarily in the top phase with a yield of 45.66% and K$\_$hGM-CSF/ of 7.64 after 2 h of incubation at room temperature.

• Steel and Composite Structures
• /
• 제24권1호
• /
• pp.93-112
• /
• 2017

Metaheuristic algorithms in general make use of uniform random numbers in their search for optimum designs. Levy Flight (LF) is a random walk consisting of a series of consecutive random steps. The use of LF instead of uniform random numbers improves the performance of metaheuristic algorithms. In this study, three discrete optimum design algorithms are developed for steel skeletal structures each of which is based on one of the recent metaheuristic algorithms. These are biogeography-based optimization (BBO), brain storm optimization (BSO), and artificial bee colony optimization (ABC) algorithms. The optimum design problem of steel skeletal structures is formulated considering LRFD-AISC code provisions and W-sections for frames members and pipe sections for truss members are selected from available section lists. The minimum weight of steel structures is taken as the objective function. The number of steel skeletal structures is designed by using the algorithms developed and effect of LF is investigated. It is noticed that use of LF results in up to 14% lighter optimum structures.

• Asian Pacific Journal of Cancer Prevention
• /
• 제14권8호
• /
• pp.4615-4619
• /
• 2013

Background: Artesunate, extracted from Artemisia annua, has been proven to have anti-cancer potential. Allicin, diallyl thiosulfinate, the main biologically active compound derived from garlic, is also of interest in cancer treatment research. This object of this report was to document synergistic effects of artesunate combined with allicin on osteosarcoma cell lines in vitro and in vivo. Methods: After treatment with artesunate and allicin at various concentrations, the viability of osteosarcoma cells was analyzed by MTT method, with assessment of invasion and motility, colony formation and apoptosis. Western Blotting was performed to determine the expression of caspase-3/9, and activity was also detected after drug treatment. Moreover, in a nude mouse model established with orthotopic xenograft tumors, tumor weight and volume were monitored after drug administration via the intraperitoneal (i.p.) route. Results: The viability of osteosarcoma cells in the combination group was significantly decreased in a concentration and time dependent manner; moreover, invasion, motility and colony formation ability were significantly suppressed and the apoptotic rate was significantly increased through caspase-3/9 expression and activity enhancement in the combination group. Furthermore, suppression of tumor growth was evident in vivo. Conclusion: Our results indicated that artesunate and allicin in combination exert synergistic effects on osteosarcoma cell proliferation and apoptosis.

• 한국미생물·생명공학회지
• /
• 제46권2호
• /
• pp.154-161
• /
• 2018

현재 도시의 도시열섬현상을 완화시키는 방안으로 도시 녹화사업 및 연구가 주목 받고 있다. 이 연구에서는 질소고정균을 분리하고, 식물 성장에 미치는 영향을 확인했다. 먼저 질소고정 균을 분리하기 위해, 질소원이 없는 배지에서 enrichment를 실시했고, 질소원이 제한된 배지에서 높은 성장을 보인 colony를 분리하여 순수분리 했다. 순수 분리된 균은 ARA를 통해 acetylene이 90% 이상 감소되고, ethylene 생성을 통해 nitrogenase의 활성을 간접적으로 확인했다. 재현성이 확인된 Cedecea sp. MK7과 Enterobacter sp. Y8을 선별했다. 선별된 질소고정 균을 perennial rye grass의 성장에 적용한 결과 건조중량이 18.65 mg인 대조군에 비해 34.80 mg (186.60%)으로 증가한 것을 확인했다. 식물 성장 후, 질소고정 균이 접종된 토양의 미생물 군집 분석은 대조군과 유사했다. 따라서 본 연구에서는 도시녹화 시스템에 질소고정 균을 이용하여 식물 성장을 촉진한다면 그 효율이 증대될 것이다.