• Preventive Nutrition and Food Science
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• 제13권3호
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• pp.196-203
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• 2008

Hwangtae, dried Alaska pollack, is a major storage product in the fish processing industry. Hwangtae is prepared by removing the internal organs and drying outdoors during the cold witner months by allowing it to thaw during the daytime and re-freeze at night under sub-zero ($-10^{\circ}C$) conditions and gradually dry from December until the next April for around 5 months from Myungtae. In this study, ground Hwangtae was hydrolyzed using two proteolytic enzymes (pepsin and alcalase) which produced five soluble active peptides from Hwangtae (yellowish dried Pollack, Theragra chalcogramma) protein. Two different peptides with strong antioxidative activity were isolated from the hydrolysate using consecutive chromatographic methods of Sephadex G-25 gel, ion-exchange chromatography on a Sepharose-Sephadex C-25 gel, and high-performance liquid chromatography. The isolated peptides, APO1 and APO2, were composed of 16 and 13 amino acid residues, respectively. Both peptides contained a Gly residue at the C-terminus and the repeating motif Gly-Pro-Hyp. The peptide with a molecular weight less than 1,000 Daltons (APACE) obtained from enzymatic hydrolysates of Hwangtae exhibited the highest ACE inhibitory activity. The APACE peptides was composed of 4 amino acid residues (Gly-Leu-Leu-Pro). These results suggest that Hwangtae hydrolysates could be a good source of peptides with ACE inhibitory activity. Biochemical analysis indicated that two 70 kDa peptides (APG1 and APG2) isolated from the hydrolysate had gelatinoytic activity, which was shown to be a calcium dependent protease type as showed by gelatin SDS PAGE.

• 미생물학회지
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• 제50권2호
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• pp.119-127
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• 2014

남극 로스해의 퇴적물로부터 배양을 통해 분리한 균주의 분류 및 생리학적 특성 분석을 수행하였다. 분리 세균 63균주의 16S rRNA 유전자 염기서열을 이용한 계통분류학적 분석 결과, 이들은 Actinobacteria, Bacteroidetes, Alphaproteobacteria 및 Gammaproteobacteria 내의 21개의 파일로타입(phylotypes)에 속하였다. 98.65% 염기서열 유사도를 기준으로, 약 49%의 균주가 잠재적으로 신종 또는 신속 후보인 것으로 나타났다. 분리된 균주 중, 각각 46%, 25% 및 32%의 균주가 세포외 단백질분해효소, 지질분해효소 및 외부다당체 생성에 대한 활성을 나타냈다. 43개의 균주는 최소 1개의 세포외 분비 물질을 생산하였고, 이들 중 21개 균주는 최소 2개의 세포외 단백질분해효소, 지질분해효소 또는/및 세포외다당체를 생성하였다. 이러한 결과는 남극 로스해 퇴적물 내의 배양된 세균 군집이 해당 환경에서 탄소와 질소와 관련된 유기물질의 가수분해에 영향을 미치고 있다는 것을 시사한다.