• Title/Summary/Keyword: cold stress

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Effects of Taste and Quality of Drugs on the Plasma Corticosterone Level in Mice exposed to Heat and Cold Stress (한약(韓藥)의 기미(氣味)가 한열자극(寒熱刺戟)을 받은 생쥐의 혈중(血中) corticosterone에 미치는 효과(效果))

  • Nam Yeo-Jeong;Lee Tae-Hee
    • Herbal Formula Science
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    • v.10 no.1
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    • pp.157-167
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    • 2002
  • This study was performed to investigate what effect the taste and quality of drugs would have on the cold and heat mechanisms of human body. We administered Hwangryeunhaedok-tang composed of bitter-tasted, cold-qualified drugs and Gungangbuza-tang composed of spice-tasted. hot-qualified drugs, respectively to the mice 1 hr before exposure to heat stress or cold stress. Plasma corticosterone level of mice was measured. The results were as follows: 1. The elevated corticosterone level in the mice exposed to heat stress was significantly decreased after administration of Gungangbuza-tang but there was no decrease after administration of Hwangryeunhaedok-tang. 2. The elevated corticosterone level in the mice exposed to cold stress was significantly decreased after administration of Hwangryeunhaedok-tang but there was mild decrease after administration of Gungangbuza-tang. 3. When the doses, 3g/kg and 1g/kg were administerd to mice exposed to heat stress. both dose showed significant decrease of corticosterone level and the dose. 3g/kg was more effective. However, in the mice exposed to cold stress, the dose, 3g/kg showed mild decrease and 1g/kg showed significant decrease. These data suggested that HW decreased the plasma corticosterone level in the mice exposed to cold stress and GB also decreased the plasma corticosterone level in the mice exposed to heat stress. In conclusion, our study revealed that the taste and quality of drugs controled the cold and heat mechanism of human body.

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THe Effect of Chronic Ehronic Treatment and Cold stress on Catecholaminergic Enzyme activity and mRNA in Rat Brain and Adrenals

  • Lee, Yong-Kyu;Park, Dong-H
    • Archives of Pharmacal Research
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    • v.19 no.5
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    • pp.374-380
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    • 1996
  • Sprague-Dawley male rats (150 g) were chronically treated with 5 v/v % ethanol admixed with nutritionally complete liquid diet and fed ad libitum for 3 weeks. One half of each group was exposed to cold stress at 4 ^{\circ}C either for 24 h (for determination of mRNA by in situ hybridization) or for 48 h (for determination of enzyme activity). Chronic ethanol treatment (ethanol) did not affect tyrosine hydroxylase(TH) mRNA level in locus coeruleus(LC) of brain and adrenal medulla(AM) compared to controls. Cold stress showed strong increase of TH mRNA level in LC and AM compared to controls. Pretreated ethanol reduced the increased TH mRNA level by cold stress in LC and AM. Ethanol did not affect TH activity in LC and adenal glands(adrenals). Cold stress increased TH activity in LC but not in adrenals. Pretreated ethanol did not reduce the increased TH activity by cold stress in LC but this result was not shown in adrenals. Phenylethanolamine-N-methyltransferase(PNMT) activity in $C_{1}$$C_{2}$ and adrenals increased only in ethanol treated group. THese results suggest that ethanol does not affect TH mRNA level and activity in LC and adrenals, but increases PNMT activity in $C_{1}$$C_{2}$ and adrenals in normal rat. It is also suggested that pretreated ethanol reduces the magnitude of cold stress response, that is induction of TH mRNA in LC and AM, and does not reduce the protein activation of TH that is also cold stress response in LC.

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Effect of Each Constituent-Herb of Sagoonja-Tang on the Change of Corticosterone Level induced by Cold Stress in Mice (Cold Stress로 유발된 생쥐의 혈중 corticosterone 농도변화에 대한 사군자탕(四君子湯) 구성약물의 효능)

  • Kwon Yong-Uk;Lee Tae-Hee
    • Herbal Formula Science
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    • v.11 no.2
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    • pp.83-94
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    • 2003
  • This study aimed to investigate the effects of each constituent-herb of Sagoonja- Tang on the plasma corticosterone level in mice exposed to cold stress. Sagoonja-Tang is composed of Ginseng Radix, Atractylodis Radix, Poria, and Glycyrrhizae Radix. Each constisuent-herb(0.5g/kg, 1.0g/kg) was administered intragastrically to mice 1hr prior to expose to forced cold water swimming($4{\circ}C$, 3min) before measuring the change of plasma corticosterone level of mice. The results were as follows: 1. At 30 minutes after the cold stress for 3 minutes the most significant increase of corticosterone level was shown. 2. Ginseng group(PGR group) didn't show any significant changes. 3. Atractylodis group(AR group) showed significant decrease at 0.5g/kg dose (P<0.01). But it didn't show significant changes at 1.0g/kg dose. 4. Poria group(P group) didn't show significant changes. But it showed slightly increase at 1.0g/kg dose. 5. Glycyrrhizae group(GR group) didn't show significant changes. These data revealed that Atractylodis macrocephalae Rhizoma might have the anti-stress effect through the control of plasma corticosterone level and the dose of herbs had a different effect on the plasma corticosterone level in mice exposed to cold stress.

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A Study on Fatigue Characteristics under Residual Stress by Cold Expansion (홀 확장 잔류응력하의 피로특성에 관한 연구)

  • Heo, Seong Pil;Kim, Cheol;Jeong, Gi Hyeon;Go, Myeong Hun;Yang, Won Ho
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.31 no.2
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    • pp.35-40
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    • 2003
  • Cold expansion method is an effective process to retard the crack initiation at a hole and to improve fatigue life by developing the compressive residual stress around the hole. Several researches have been treated for the crack propagating toward a hole around which the residual stress by cold expansion exists. The objective of this paper is to evaluate the fatigue characteristics under the residual stress by cold expansion. Fatigue tests are performed for the plate with a central hole to which cold expansion has been applied, and the effects of the residual stress on the fatigue life are evaluated. Also the fatigue crack growth tests are conducted for the CT specimen in which the residual stress by cold expansion has been generated. The change of fatigue crack growth characteristics by the residual stress is investigated and the effects of cold expansion ratio are evaluated.

The Finite Element Analysis for Prediction of Residual Stresses Induced by Cold Expansion (홀확장 잔류응력 예측을 위한 유한요소해석)

  • Kim, Cheol;Yang, Won-Ho;Ko, Myung-Hoon;Heo, Sung-Pil;Hyun, Cheol-Seung
    • Proceedings of the KSME Conference
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    • 2000.11a
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    • pp.470-474
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    • 2000
  • Cold expansion of fastener holes is a mechanical process widely used in the aerospace industry. This treatment leads to an improvement of fatigue behavior due to the developed compressive residual stresses on the hole surface. The residual stress profile depends on the parameters of cold expansion, which are, expanding rate, inserting direction of mandrel, material properties etc. and the method to confirm this profile is only measurement by X-ray diffractometer. Despite its importance to aerospace industries, little attention has been devoted to the accurate modelling of the process. In this paper, Two-dimensional axisymmetric finite element simulations have been conducted for the cold expansion in an aluminium plate in order to predict the magnitude and distribution of the residual stress and plastic deformation. Maximum compressive residual stress could be increase about 7 percent using the 2-step cold expansion method.

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Residual stress of cold-formed thick-walled steel rectangular hollow sections

  • Zhang, Xingzhao;Liu, Su;Zhao, Mingshan;Chiew, Sing-Ping
    • Steel and Composite Structures
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    • v.22 no.4
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    • pp.837-853
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    • 2016
  • This paper presents the experimental and numerical study on the distribution of transverse and longitudinal residual stresses in cold-formed thick-walled structural steel rectangular hollow sections manufactured by indirect technique. Hole-drilling method is employed to measure the magnitude of the transverse and longitudinal surface residual stress distribution, and the effects of the residual stresses are evaluated qualitatively by sectioning method. It is shown that compared to normal cold-formed thin-walled structural hollow sections (SHS), the cold-formed thick-walled SHS has similar level of residual stress in the flat area but higher residual stresses in the corner and welding areas. Both the transverse and longitudinal residual stresses tend to open the section. In order to predict the surface residual stresses in the corners of the cold-formed thick-walled SHS, an analytical model is developed. 2D finite element simulation of the cold bending process is conducted to validate the analytical approach. It is shown that in analyzing bending for thick-walled sections, shifting of neutral axis must be considered, since it would lead to nonlinear and non-symmetrical distribution of stresses through the thickness. This phenomenon leads to the fact that cold-formed thick-walled SHSs has different distribution and magnitude of the residual stresses from the cold-formed thin-walled SHSs.

Immunopotentiating Effect of Protein Extract from Dioscorea quinqueloba in Stressed Mice (단풍마 단백질 추출물의 스트레스로 인한 면역력 저하 개선 효과)

  • Kim, Ju Hwan;Lee, Sun-Mee;Lee, Dong-Cheol
    • The Korean Journal of Food And Nutrition
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    • v.31 no.2
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    • pp.252-257
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    • 2018
  • It is noted that Dioscorea quinqueloba is a medicinal herb that is widely used to treat cardiovascular disease and is assessed as useful to treat other various medical conditions. The immunopotentiating effects of the protein extract (DQP-1) from Dioscorea quinqueloba were thus formally investigated in vivo under incident of cold stress. In this case study, the spleen and thymus weight in mice was shown to have decreased after a measured exposure to cold stress, while the adrenal gland weight in the mice was shown to have increased. The systematic oral administration of DQP-1 significantly recovered the weight loss of the spleen and suppressed the adrenal gland hypertrophy during the association with cold stress. Additionally, the DQP-1 also restored the ascorbic acid level in the adrenal gland reduced after cold stress. The cold stress exposure lowered the percentage of $CD4^+$ and $CD8^+$ cells in the mouse thymus as determined by the flow cytometric analysis, as well as the levels of some serum immunological cytokines(interleukin-2, interleukin-12, and interferon-${\gamma}$) in the studied mice. The resulting identified weakened immunity caused by cold stress was also recovered by a treatment with DQP-1. The DQP-1 significantly suppressed the formation of serum enzymes of alanine aminotransferase, aspartate aminotransferase, and lactate dehydrogenase, which were systematically elevated during the cold stress episode. These results indicate that DQP-1 can improve immunity in mice that are characteristically weakened under stress.

Identification of the genes which related cold (low temperature) stress in Bombyx mori

  • Kang, Min-Uk;Choi, Kwang-Ho;Park, Kwan-Ho;Nho, Si-Kab
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.2
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    • pp.102-107
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    • 2016
  • Stress may be defined as any modification of environmental parameters that leads to a response by biological organisms. Stresses that affect biolpgical structures may be nonthermal, such as ultraviolet radiation, pH, or salinity, or thermal. Temperture is one of the major stresses that all living organism face. The major effects of cold(low emperature) are decrease of membrane fluidity and the stabilization of secondary structures of RNA and DNA in the cells, which may effect the efficiency of translation, transcription, and DNA replication. In this study, we focus on discovering the genes that are expressed by the cold(low temperature) stress in the silkworm. In cold(low temperature) stress test, we found 100% survive from cold stress at $0^{\circ}C$ up to 12h and $-5^{\circ}C$ up to 2h, and then, survive rate was rapidly decrease in silkworm. Thereafter two whole genes have selected by SSH(Suppression subtractive hybridization). (GenBank accession : GQ149511, GQ338156)

CaPUB1, a Hot Pepper U-box E3 Ubiquitin Ligase, Confers Enhanced Cold Stress Tolerance and Decreased Drought Stress Tolerance in Transgenic Rice (Oryza sativa L.)

  • Min, Hye Jo;Jung, Ye Jin;Kang, Bin Goo;Kim, Woo Taek
    • Molecules and Cells
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    • v.39 no.3
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    • pp.250-257
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    • 2016
  • Abiotic stresses such as drought and low temperature critically restrict plant growth, reproduction, and productivity. Higher plants have developed various defense strategies against these unfavorable conditions. CaPUB1 (Capsicum annuum Putative U-box protein 1) is a hot pepper U-box E3 Ub ligase. Transgenic Arabidopsis plants that constitutively expressed CaPUB1 exhibited drought-sensitive phenotypes, suggesting that it functions as a negative regulator of the drought stress response. In this study, CaPUB1 was over-expressed in rice (Oryza sativa L.), and the phenotypic properties of transgenic rice plants were examined in terms of their drought and cold stress tolerance. Ubi:CaPUB1 T3 transgenic rice plants displayed phenotypes hypersensitive to dehydration, suggesting that its role in the negative regulation of drought stress response is conserved in dicot Arabidopsis and monocot rice plants. In contrast, Ubi:CaPUB1 progeny exhibited phenotypes markedly tolerant to prolonged low temperature ($4^{\circ}C$) treatment, compared to those of wild-type plants, as determined by survival rates, electrolyte leakage, and total chlorophyll content. Cold stress-induced marker genes, including DREB1A, DREB1B, DREB1C, and Cytochrome P450, were more up-regulated by cold treatment in Ubi:CaPUB1 plants than in wild-type plants. These results suggest that CaPUB1 serves as both a negative regulator of the drought stress response and a positive regulator of the cold stress response in transgenic rice plants. This raises the possibility that CaPUB1 participates in the cross-talk between drought and low-temperature signaling pathways.

Effect of Dietary Lipids and Stress on Neurotransmitters in Rats (식이지방과 스트레스가 신경전달물질의 수준에 미치는 영향)

  • 한효나
    • Journal of Nutrition and Health
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    • v.29 no.5
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    • pp.472-479
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    • 1996
  • To investigate the effect of dietary lipids and stress on brain catecholamine and serotonin concentration, sixty three weanling male Sprague-Dawley rats(mean body weight$\pm$SD : 171$\pm$3g) were fed a diet containing fish oil, soybean oil or beef tallow and than, each was exposed to three different types of stress, isolated, grouped or cold, respectively. Cold stress seemed to be most severe and living together in a large cage with some playing equipments is more stressful than living alone in a classical small cage evidenced by plasma corticosterone level. Average food intake and body weight gain were not significantly different among exprimental groups. In adrenal catecholamines, norepinephrine was significantly affected by diet and stress and dopamine was by stress. Norepinephrine concentration of the fish oil group was lowest among diet groups. Adrenal epinephrine, however, was not. It was also shown than the cold stress significantly increased the brain norepinephrine concentration. The cold stress significantly induced higher content of brain serotonin than the grouped stress. However, the concentratin of 5-hydroxyindoleacetic acid(5-HIAA), the metabolite of serotonin, was not significantly different among groups. Therefore, this results suggest that stress affects sympathetic neuronal activity, and fish oil might lighten the burden of stress.

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