• Korean Journal of Veterinary Research
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• v.45 no.2
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• pp.185-189
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• 2005

Actinobacillus pleuropneumoniae is the causative agent of a porcine contagious pleuropneumonia. Among several virulence factors including exotoxin (Apx toxins), LPS, transferrin-binding proteins, OMPs, and some proteases, Apx toxins have been major targets for the protection study. In this study, cloning and expression of A. pleuropneumoniae Apx I and Apx II toxin, which are produced by all highly virulent strains, were performed by Escherichia coli expression system. Genes coding Apx I and II toxin were amplified from the A. pleuropneumoniae serotype 5 genomic DNA using polymerase chain reaction and cloned to a prokaryotic expression vector, pRSET. Expression of the Apx I and Apx II coding sequences in E. coli resulted in the formation of insoluble inclusion bodies purified according to a denaturing purification protocol, which employs the use of guanidium. Recombinant proteins were purified using $Ni^{2+}$-charged resin affinity purification. This expression and purification system made it possible to produce Apx I and Apx II in large amounts for further immunologic studies.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.43 no.4 s.310
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• pp.96-107
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• 2006

In this paper, a low bitrate video coding method based on new panoramic modeling is proposed for panning cameras. An input video frame from a panning camera is decomposed into a background image, rectangular moving object regions, and a residual image. In coding the background, we employ a panoramic model that can account for several image formation processes, such as perspective projection, lens distortion, vignetting and illumination effects. Moving objects aredetected, and their minimum bounding rectangular regions are coded with a JPEG-2000 coder. We have evaluated the effectiveness of the proposed algorithm with several indoor and outdoor sequences and found that the PSNR is improved by $1.3{\sim}4.4dB$ compared to that of JPEG-2000.

• Journal of Aquaculture
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• v.22 no.1
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• pp.83-91
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• 2009

The Korean doty barbel Hemibarbus mylodon (Cypriniformes; Cyprinidae) is a critically endangered freshwater fish species mainly because of its natural habitat degradation. Three full-length complementary DNA (cDNA) clones representing different muscle actin isoforms were isolated and characterized. The three muscle actin isoforms were 1,294-1,601 bp long with the identical open reading frames of 1,134 bp with the deduced amino acid residues of 377. They showed 83.9-87.2% identities in the coding nucleotide level and 96.8-98.1% identities in the amino acid level. Phylogenetic analysis with the coding nucleotide sequences revealed that three muscle actin isoforms of H. mylodon formed strongly supported monophyletic groups with one of cypriniform skeletal $\alpha$-actin (acta1), cypriniform aortic $\alpha$-actins (acta2), and uncharacterized Danio rerio muscle actin isoform/Salmo trutta slow muscle actin (a novel muscle actin type). Our phylogenetic tree further suggested that cypriniform acta2 only showed the orthologous relationship to tetrapod acta2. Other multiple actin isoforms from diverse teleostean taxa were however clustered to no tetrapod orthologs, i.e., acta1, cardiac $\alpha$-actins (aetc1), acta2, and enteric $\gamma$-actin (actg2). This result strongly suggested that teleostean muscle actins have experienced different and complicated evolutionary history in comparison to mammalian counterparts.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.2
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• pp.157.2-157.2
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• 2012

Direct-conversion receivers often suffer from a DC-offset that is a by-product of the direct conversion process to baseband. In general, a basic approach to reduce the DC-offset is to do simple average of the baseband signal and remove the DC by subtracting the average. However, this gives rise to a residual DC offset which degrades the performance when the receiver adopts the coding schemes with high coding rates such as 8-PSK. Therefore, more advanced methods should be additionally required for better performance. While the training sequences are basically designed to have good auto-correlation properties to facilitate the channel estimation, they may be not good for the simultaneous estimation of the channel response and the DC-offset. Also the DC offset compensation under a bad condition does not give good results due to the estimation error. Correspondingly, the proposed scheme employs the two important points. First, the training sequence codes are divided into two groups by MSE(Mean Squared Errors) for estimating the channel taps and then SNR calculated from each group is compared to predefined threshold to do fine DC-offset estimation. Next, ON/OFF module is applied for preventing performance degradation by large estimation error under severe channel conditions. The simulation results of the proposed scheme shows good performances compared to the existing algorithm. As a result, this scheme is surely applicable to the receiver design in many communications systems.

• Archives of Pharmacal Research
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• v.19 no.6
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• pp.497-501
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• 1996

A gene coding for triosephosphate isomerase (TPI) from a rat skeletal muscle cDNA library was cloned and its nucleotide sequence was determined. The 1, 348-bp cDNA clone contains 24 bp $5^I$ noncoding region, the entire 750 bp coding region corresponding to a protein of 249 amino acids, $547bp 3^I$ noncoding region and part of a poly(A) tail. It also contains a polyadenylation signal, AATAAA, starting from 17 bp upstream of the poly(A) tail. The calculated molecular weight of rat TPI is 27.8 kDa and the net charge is +4. The deduced amino acid sequence from rat TPI CDNA sequence has 93% and 94% homology with that of mouse and human clones, respectively. The amino acids at the residue of Asn12, Lys14, His96, Glu 166, His96, His101, Ala177, Tyr165, Glu13O, Tyr2O9, and Ser212 in catalytic site are completely identical, confirming that the functional residues in TPI proteins are highly conserved throughout evolution. The most profound characteristic of rat TPI enzyme, compared with other TPIs, is that there are five cysteine substitutions at the residue of 21, 27, 159, 195 and 204. A Glu123 instead of Gly was found in rabbit, rhesus, mouse and human sequences. Through the method of RT-PCR, the mRNA transcription level of TPI gene was found to be different among various tissues and was highest in muscle.

• Journal of Information Processing Systems
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• v.11 no.3
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• pp.483-494
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• 2015

High Efficiency Video Coding (HEVC) is the most recent video codec standard of the ITU-T Video Coding Experts Group and the ISO/IEC Moving Picture Experts Group. The main goal of this newly introduced standard is for catering to high-resolution video in low bandwidth environments with a higher compression ratio. This paper provides a performance comparison between HEVC and H.264/AVC video compression standards in terms of objective quality, delay, and complexity in the broadcasting environment. The experimental investigation was carried out using six test sequences in the random access configuration of the HEVC test model (HM), the HEVC reference software. This was also carried out in similar configuration settings of the Joint Scalable Video Module (JSVM), the official scalable H.264/AVC reference implementation, running on a single layer mode. According to the results obtained, the HM achieves more than double the compression ratio compared to that of JSVM and delivers the same video quality at half the bitrate. Yet, the HM encodes two times slower (at most) than JSVM. Hence, it can be concluded that the application scenarios of HM and JSVM should be judiciously selected considering the availability of system resources. For instance, HM is not suitable for low delay applications, but it can be used effectively in low bandwidth environments.

• Korean Journal of Microbiology
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• v.41 no.4
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• pp.306-311
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• 2005

In this study, the amino-terminal half truncated lump and the whole lump genes from Photobacterium phosphoreum coding for the lumazine protein were cloned by polymerase chain reaction and expressed in Escherichia coli. To identifiy of the binding site of the ligand or substrate, the amino acid identities from the sequences of the lumazine protein, yellow fluorescent protein, and riboflavin synthase from different organisms were also compared and analyzed.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.9
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• pp.4587-4605
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• 2019

High Efficiency Video Coding (HEVC) suffers from high computational complexity due to its quad-tree structure in motion estimation (ME). This paper exposes an adaptive search range decision algorithm for accelerating HEVC integer-pel ME on GPU which estimates the optimal search range (SR) using a MAP (Maximum A Posteriori) estimator. There are three main contributions; First, we define the motion feature as the standard deviation of motion vector difference values in a CTU. Second, a MAP estimator is proposed, which theoretically estimates the motion feature of the current CTU using the motion feature of a temporally adjacent CTU and its SR without any data dependency. Thus, the SR for the current CTU is parallelly determined. Finally, the values of the prior distribution and the likelihood for each discretized motion feature are computed in advance and stored at a look-up table to further save the computational complexity. Experimental results show in conventional HEVC test sequences that the proposed algorithm can achieves high average time reductions without any subjective quality loss as well as with little BD-bitrate increase.

• Genomics & Informatics
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• v.21 no.4
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• pp.51.1-51.5
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• 2023

The genus Neoarius, known as marine catfish, is a group of the family Ariidae, composed of 10 species found in Oceania. None of the species in this genus have their mitochondrial genome described, which is highly valuable in phylogenetic and molecular evolution studies. For the present work, eight species from the Neoarius genus were selected: Neoarius utarus, Neoarius midgleyi, Neoarius graeffei, Neoarius leptaspis, Neoarius berenyi, Neoarius paucus, Neoarius pectoralis, and Neoarius aff. graeffei. DNA sequences of the eight species were obtained through the NCBI Sequence Read Archive (SRA) database, and the mitochondrial genomes were assembled using the NOVOplasty tool on the Galaxy platform, subsequently annotated with the MitoAnnotator tool. We then utilized the protein-coding genes from the mitogenomes to estimate the phylogenetic relationships within the group, including seven additional mitogenomes available in the NCBI. In all species, the mitochondrial genomes presented 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 D-loop.

• Journal of Broadcast Engineering
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• v.22 no.4
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• pp.484-495
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• 2017

The development of display devices and the increase of network transmission bandwidth bring demands for over 2K high resolution video such as panorama video, 4K ultra-high definition commercial broadcasting, and ultra-wide viewing video. To compress these image sequences with significant amount of data, High Efficiency Video Coding (HEVC) standard with the highest coding efficiency is a promising solution. HEVC, the latest video coding standard, provides high encoding efficiency using various advanced encoding tools, but it also requires significant amounts of computation complexity compared to previous coding standards. In particular, the complexity of HEVC decoding process is a imposing challenges on real-time playback of ultra-high resolution video. To accelerate the HEVC decoding process for ultra high resolution video, this paper introduces a data-level parallel video decoding method using slice and/or tile supported by HEVC. Moreover, deblocking filter process is further parallelized. The proposed method distributes independent decoding operations of each tile and/or each slice to multiple threads as well as deblocking filter operations. The experimental results show that the proposed method facilitates executions up to 2.0 times faster than the HEVC reference software for 4K videos.