• Title/Summary/Keyword: code sequence

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Optimization for Production of Exo-β-1,3-glucanase (Laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae (Saccharomyces cerevisiae에서 Aspergillus oryzae 유래의 exo-β-1,3-glucanase (laminarinase)의 생산 최적화)

  • Kim, Min-Jung;Nam, Soo-Wan;Tamano, Koichi;Machida, Masayuki;Kim, Sung-Koo;Kim, Yeon-Hee
    • KSBB Journal
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    • v.26 no.5
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    • pp.427-432
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    • 2011
  • In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.

A Study on the Implementation of Direct Digital Frequency Synthesizer using the synthesized Clock Counting Method to make the State of randomly Frequency Hopping (주파수 도약용 표본클럭 합성 계수 방식의 직접 디지틀 주파수 합성기 구현에 관한 연구)

  • 장은영;이성수;김원후
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.16 no.10
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    • pp.914-924
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    • 1991
  • It has been generally used for PLL(Phase Locked Loop) to be synthesized randomly chosen frequency state, but the PLL locking time was inevitable element. A direct digital synthesizer. Which makes output frequency directly in sine wave by a phase accumulating method, could be leiminate the defect, although a phase distortion in frequency spectrum. In order to improve this disadvantage, the phase accumulating method is reconsidered in the side of he output wave formula expression. A new mechanism is proposed, and it is constructed by a most suitable logic elements. The spectrum of synthesized sine waveform is simulated and compared with a measured value, and it’s the coherence frequency hoppong state with the PN(Pseudo Noise) code sequence is confirmed. In this results, the power levels of phase distortion harmonics are decreased to 10~25dB and bandwidths are increased to 420kHz.

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Blind MOE Interference Canceller for Multicarrier DS-CDMA Systems (다중 반송파 DS-CDMA 시스템을 위한 블라인드 MOE 간섭 제거기)

  • Woo Dae Ho;Byun Youn Shik
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.10C
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    • pp.1395-1401
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    • 2004
  • In The objective of this paper is to apply blind minimum output energy multiuser detection method to multicarrier direct sequence code division multiple access system. The performance of MC/DS-CDMA is reduced due to multiple access. To increase the performance of system, we need to cancel multiple user components. Blind interference canceller is able to detect the desired's information with the only minimal information of the desired user. We evaluate the performance of the proposed receiver under Gaussian channel. Simulation results show that the proposed receiver has about 6[dB] gain of signal to noise ratio without multiple user interference and has about 3[dB] gain of SNR with multiple user interference. Also, the result of comparing the capacity of the active users shows that the proposed method has about 2 times capacity more than conventional method. As a conclusion, simulation results show that the proposed method has better performs better than conventional method.

Analysis of Linear Span of Non-linear Binary Sequences with Decimation d=2m-2(2m+3) (데시메이션이 d=2m-2(2m+3)인 비선형 이진수열의 선형스팬 분석)

  • Yim, Ji-Mi;Cho, Sung-Jin;Kim, Han-Doo;Kim, Seok-Tae
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.18 no.3
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    • pp.609-616
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    • 2014
  • Large linear span makes difficult to predict, so this study is important to the security and code system. It has been studied about the non-linear binary sequences having low correlation values and large linear span. In this paper we analyze the linear span of $S^r_a(t)=Tr^m_1\{[Tr^n_m(a{\alpha}^t+{\alpha}^{dt})]^r\}$ ($a{\in}GF(2^m)$, $0{\leq}t{\leq}2^m-2$) where n=2m and $d=2^{m-2}(2^m+3)$.

Verification for Multithreaded Java Code using Java Memory Model (자바 메모리 모델을 이용한 멀티 스레드 자바 코드 검증)

  • Lee, Min;Kwon, Gi-Hwon
    • The KIPS Transactions:PartD
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    • v.15D no.1
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    • pp.99-106
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    • 2008
  • Recently developed compilers perform some optimizations in order to speed up the execution time of source program. These optimizations require the reordering of the sequence of program statements. This reordering does not give any problems in a single-threaded program. However, the reordering gives some significant errors in a multi-threaded program. State-of-the-art model checkers such as JavaPathfinder do not consider the reordering resulted in the optimization step in a compiler since they just consider a single memory model. In this paper, we develop a new verification tool to verify Java source program based on Java Memory Model. And our tool is capable of handling the reordering in verifying Java programs. As a result, our tool finds an error in the test program which is not revealed with the traditional model checker JavaPathFinder.

Parametric Study on Long-Term Deflections of Flat Plates Considering Effects of Construction Loads and Cracking (시공하중 및 균열 효과를 고려한 플랫 플레이트의 장기 처짐에 대한 변수 연구)

  • Choi, Seung Min;Eom, Tae Sung;Kim, Jea Yo
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.16 no.1
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    • pp.44-54
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    • 2012
  • The structural designs of RC flat plates that have no flexural stiffness by boundary beams may be governed not by strength conditions but by serviceabilities. Specially, since over-loading and tensile cracking in early-aged slabs significantly increase the immediate and long-term deflections of a flat plate system, a construction sequence and its impact on the slab deflections may be decisive factors in designs of flat plate systems. In this study, the procedure of calculating slab deflections with considering construction sequences, concrete cracking, and long-term effects is proposed. Using the proposed method, the parametric study for deflections of flat plates is performed. With various conditions for slab construction cycle, the number of shored floors, tensile or compressive reinforcement ratio, compressive strength of concrete, construction live load, and slab thickness, the immediate deflection during construction and long-term deflections after completion are analyzed. The calculated results are compared with the serviceability limits offered by the structural design code.

A Study of Acquisition and Analysis on the Bios Firmware Image File in the Digital Forensics (디지털 포렌식 관점에서 BIOS 펌웨어 이미지 파일 수집 및 분석에 관한 연구)

  • Jeong, Seung Hoon;Lee, Yun Ho;Lee, Sang Jin
    • KIPS Transactions on Computer and Communication Systems
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    • v.5 no.12
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    • pp.491-498
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    • 2016
  • Recently leakages of confidential information and internal date have been steadily increasing by using booting technique on portable OS such as Windows PE stored in portable storage devices (USB or CD/DVD etc). This method allows to bypass security software such as USB security or media control solution installed in the target PC, to extract data or insert malicious code by mounting the PC's storage devices after booting up the portable OS. Also this booting method doesn't record a log file such as traces of removable storage devices. Thus it is difficult to identify whether the data are leaked and use trace-back technique. In this paper is to propose method to help facilitate the process of digital forensic investigation or audit of a company by collecting and analyzing BIOS firmware images that record data relating to BIOS settings in flash memory and finding traces of portable storage devices that can be regarded as abnormal events.

Method Discrimination for Product Traceability and Identification of Korean Native Chicken using Microsatellite DNA (초위성체를 이용한 한국 재래닭의 원산지 추적 및 개체 식별 방법에 관한 연구)

  • Park, Mi-Hyun;Oh, Jae-Don;Jeon, Gwang-Joo;Kong, Hong-Sik;Sang, Byong-Don;Choi, Chull-Hwan;Yeon, Sung-Hum;Cho, Byong-Wok;Lee, Hak-Kyu
    • Korean Journal of Organic Agriculture
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    • v.12 no.4
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    • pp.451-461
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    • 2004
  • In an animals, identification system has been widely used by ear tag with dummy code and blood typing for parernity. Also, genotyping methods were using for useful mean of individual identification for live animals. In the case of genotyping estimation of gene in population of korean native chicken. In this study, we tested for development of genetic markers used it possible to determination of individual identification system. The candidate genetic markers were used already bow 10 of microstalite DNA sequence information in chromosome No. 1 and 14. Result of analysis for genotyping, the number of alleles of those microstatelites DNA was shown minimal 3 to 12 and the heterozygote expression frequency range was shown from 0.617 to 0.862. In our result, effective number of allele for each microsatellites DNA was shown 3~7, and the accuracy of individual identification was shown nearly 100%, when used with 6 genetic marker. This study was about genotyping method for identification used specific genetic marker form microsatellite DNA in the brand marketing of korean native chicken. Our results suggest that genotyping method used specific genetic marker from microsatellite DNA might be very useful for determination of individual identification.

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A Study on Phase Error of Orthogonal MC DS-CDMA Using Hybrid SC/MRC-2/4 (하이브리드 SC/MRC-2/4기법을 적용한 직교 MC DS-CDMA 시스템의 위상 에러에 관한 연구)

  • Kim, Won-Sbu
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.11 no.9
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    • pp.1734-1741
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    • 2007
  • In this paper, the Hybrid SC/MRC-2/4 method in which bit synchronization and phase synchronization were not required was applied to the orthogonal MC DS-CDMA system in which each normalized subcarrier interval and processing gain had the same value, respectively, and the direct sequence spread code of each subcarrier was orthogonal. In the broadband wireless system in which multi-carrier transmission was used, a Doppler frequency shift occurred, which was caused by the difference between the highest subcarrier frequency md the lowest subcarrier frequency. In order to complement phase error caused by the shift, the orthogonal MC DS-CDMA system was analyzed so that the receiving signal could be perfectly synchronized by adjusting the PLL gain suitable for the entire system. As a result of simulations, as the PLL gain was increased, the change in the intervals was close to the case of perfect synchronization however, it became less when the PLL gain reached more than a certain value. Therefore, by selecting a proper PLL gain suitable for the system the orthogonal MC DS-CDMA can be designed in which the Hybrid SC/MRC method is applied.

On algorithm for finding primitive polynomials over GF(q) (GF(q)상의 원시다항식 생성에 관한 연구)

  • 최희봉;원동호
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.11 no.1
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    • pp.35-42
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    • 2001
  • The primitive polynomial on GF(q) is used in the area of the scrambler, the error correcting code and decode, the random generator and the cipher, etc. The algorithm that generates efficiently the primitive polynomial on GF(q) was proposed by A.D. Porto. The algorithm is a method that generates the sequence of the primitive polynomial by repeating to find another primitive polynomial with a known primitive polynomial. In this paper, we propose the algorithm that is improved in the A.D. Porto algorithm. The running rime of the A.D. Porto a1gorithm is O($\textrm{km}^2$), the running time of the improved algorithm is 0(m(m+k)). Here, k is gcd(k, $q^m$-1). When we find the primitive polynomial with m odor, it is efficient that we use the improved algorithm in the condition k, m>>1.