• 식물조직배양학회지
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• 제22권5호
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• pp.299-306
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• 1995

본 실험은 강활(O. koreanum)과 지리강활(A.purpuraefolia)의 체세포배 발생을 위한 효율적인 세포배양시스템을 확립하고자 실시하였다. 강활과 지리강활의 개화후 3주된 미숙종자는 0.1 mg/L 2,4-D와 0.1 mg/L BA 혼용처리구에서 배발생캘러스와 체세포배 발생이 가장 양호하였고, 0.1-3.0mg/L NAA가 처리된 배지에서 재분화된 식물체의 자엽과 배축의 가장자리로부터 직접 체세포배가 발생하였다. 미숙종자의 휴면을 고려하여 저온처리한 결과, 5$^{\circ}C$에서 10일동안 저장 후, 25$\pm$2$^{\circ}C$에서 배양하였을 때 반응이 없던 배양체로부터 배발생 캘러스와 체세포배가 발생하였고 이 체세포배는 줄기와 뿌리로 분화되었다. 지리강활에서는 배지내 첨가한 glutamine과 coconut milk의 뚜렷한 효과가 인정되지 않았다. 그러나 강활의 경우에는 대조구에 비하여 glutamine과 10% coconut milk가 포함된 배지에서 체세포배 발생률이 항상되었고 특히 glutamine은 coconut milk에 비해 NH$_4$NO$_3$ 대체효과가 인정되었다. 체세포배의 器內 식물체전환율은 MS 기본배지에서 89.1%로 가장 양호하였고 배발생 캘러스의 증식용 배지로는 0.01 mg/L 2,4-D와 0.01 mg/L BA혼용처리구에서 가장 좋았다.

• 한국식품과학회지
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• 제50권1호
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• pp.92-97
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• 2018

말차 첨가 코코넛밀크 스프레드의 항산화 활성과 품질특성을 측정하였다. 본 실험의 스프레드 제조 시 말차 첨가량은 각각 S1(0 g), S2 (4 g), S3 (8 g), S4 (12 g), S5 (16 g)으로 하였다. 품질특성 결과 수분, L값, a값, pH의 경우 말차 첨가량이 증가할수록 측정값은 감소하였다. 반면 점도, b값의 경우 말차 첨가량이 증가할수록 측정값은 증가하였다. 동량의 설탕을 첨가한 각 군의 당도 범위는 모든 군이 $2.70-3.27^{\circ}Bx$ 사이에 속하였다. 관능검사는 색, 맛(단맛, 쓴맛), 조직감(부착성, 발림성), 전반적인 기호도 항목을 평가하였다. 그 결과, 8 g의 말차를 첨가한 S3 스프레드가 가장 선호도가 높았다. 말차 분말의 항산화 활성에서 총 폴리페놀 함량과 총 플라보노이드 함량은 각각 226 mg GAE/g, 809.42 mg QE/g이였다. 또한 DPPH 라디칼 소거능과 환원력은 $5.76{\mu}g/mL$ ($IC_{50}$), 2.26 O.D.으로 측정되었다. 말차 첨가 코코넛밀크 스프레드의 항산화 활성은 말차 첨가량이 높을수록 증가함을 알 수 있다.

• Asian-Australasian Journal of Animal Sciences
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• 제11권5호
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• pp.586-596
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• 1998

The relevance and importance of buffalo production under coconut plantations in the North Westen Province of Sri Lanka was studied in three districts (Bingiriya, Pannala, Kuliyapitiya). The objective of the study was to collect baseline information on socioeconomic and cultural aspects of buffalo production, with a view to promote and disseminate new technologies. The survey technique used consisted of a formal survey using a structured questionnaire (71 households) and rapid appraisal (55 households). The results indicate the existence of a wide stratification of dariy farmers which ranged from skilled dairy operators with high levels of production and management of efficiency to marginal subsistence farmers with low levels of productivity. The most frequent family size of households ranged from 4-5 members (58%), and the average family size was 4.7. The actual average land ownership accounts to 2.4 ha of upland and 0.5 ha of lowland, but when their accessibility to common property resources are taken into account, the land availability was assessed at 13 ha and 0.7 ha of upland and lowland, respectively. The highest average monthly income (Rs. 13,590) was received by farmers with off-farm employment (primary) who are also engaged in livestock production (secondary), and livestock contributed 43% of the total income. Livestock farmers who practised integrated crop farming as a secondary source of income received a monthly income of Rs. 10,843, and those involved in crop production as the primary source received the lowest average income (Rs. 7,295). The survey revealed a high investment cost on concentrate feeds (47%) for milk production. However some farmers obtained higher milk yields (11 litres/cow/day) at lower ration costs, and this could be attributed to the entrepreneurship skills and management efficiency. The study area had a well developed market infrastructure for fresh milk, principally due to the existence of the Nestle's company and the Coconut Triangle Milk Union. On an average the producer collected Rs. 10 per litre of milk marketed.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.18-26
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• 2004

This study evaluated the impact of an AI heifer calf rearing scheme on dairy stock development, in a coconut grazing and a peri-urban smallholder dairy production system in the Western Province of Sri Lanka. The heifer rearing scheme included free advice on calf rearing, drugs, acaricides, minerals and subsidised concentrates for 30 months. The farmers in the coconut growing area integrate dairying with their plantation, they sell their milk to the main processors. The peri-urban farmers are intensive milk producers, who sell their milk at informal markets. To estimate the effect of the heifer rearing scheme on dairy replacement stock development, scheme farmers were compared with farmers who did not participate in the scheme. Calf mortality was twice as high in non-scheme farms (23-28%) as in scheme farms (12-14%). The scheme had a positive effect on weight development and scheme heifers calved 4.5 months earlier than non-scheme heifers. The calf rearing package is cost effective in both farming systems, however, the required cash inputs are a major constraint. The costs per in-calf heifer under the scheme are much lower than the production of such animals by either multiplication in state farms or importing them. The coconut grazing system showed the highest potential for producing surplus dairy stock.

• Asian-Australasian Journal of Animal Sciences
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• 제13권1호
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• pp.60-67
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• 2000

A survey involving 71 cattle and buffalo farming households under coconut plantations was carried out in three districts (Pannala, Bingiriya and Kuliyapitiya) with the aim of assessing the status of livestock farming. Also, 24 households (eight from each district) were visited monthly for period of one year to collect information on feeding practices. Apart from milk, animals were reared for selling, draught, bio-gas and for manure. Due to difference in system of management of cattle and buffaloes, manure from buffaloes (46%) was more frequently used for coconuts than that from cattle (10%). Majority of cattle were improved breeds (temperate origin) or their crosses, as compared to buffaloes (mainly indigenous). The most predominant management system was tethered grazing during the day, and stall feeding during the night. Coconut land (own or others) and paddy fields were the major grazing areas for the animals. The grass from coconut land was lower in crude protein (8.2%) and digestibility (48%) compared to those from paddy fields (12.1 and 57%, respectively). Of the 288 rations analysed, grass was included in 280 of the daily rations for cattle as compared to 251 for buffaloes. Straw was more commonly included in mixed rations for buffaloes (137 out of 288) than for cattle (53 out of 288). The frequency of use of straw for buffaloes was high in Pannala (75 out of 137 cases). There was wide variation among the improved breeds of cattle and buffaloes in milk production (2 to 9 litres/day), lactation length (6 to 10 months) and calving interval (13-21 months).

• 한국축산식품학회지
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• 제35권2호
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• pp.197-204
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• 2015

This study was performed to investigate the effect of palm or coconut solid lipid nanoparticles (PO-SLNs or CO-SLNs) on growth of Lactobacillus plantarum (L. plantarum) in milk during storage period. The PO or CO (0.1% or 1.0%) was dispersed both in distilled water (DW) and ultra high temperature milk (UHTM), and subsequently emulsified with Tween^® 80 by ultrasonication (30% power, 2 min). Increase in particle size and encapsulation efficiency (EE%) in DW was observed with an increase in oil concentration, whereas a decrease in ζ-potential of SLNs was noted with an increment in oil concentration. Moreover, the CO-SLNs exhibited relatively smaller particle size and higher EE% than PO-SLNs. The CO-SLNs were found to be more stable than PO-SLNs. Higher lipid oxidation of PO or CO-SLNs in UHTM was observed during the storage test, when compared to PO or CO-SLNs in DW. However, there was no remarkable difference in lipid oxidation during storage period (p>0.05). In the growth test, the viability of L. plantarum in control (without PO or CO-SLNs in DW) exhibited a dramatic decrease with increasing storage period. In addition, viability of L. plantarum of PO or COSLNs in UHTM was higher than that of SLNs in DW. Based on the present study, production of SLNs containing PO or CO in UHTM is proposed, which can be used in lactobacilli fortified beverages in food industry.

• 한국미생물·생명공학회지
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• 제30권1호
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• pp.57-62
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• 2002

Acetobacter xylinum GS11 균주를 이용하여 다양한 배양조건에서 미생물 셀룰로오스의 생산성을 검토하였다. 탄소원으로 glucose 이외에 첨가한 기질은 영향이 없었으며, 2% glucose 농도 범위에서 셀룰로오스 생산량이 2.8 g/L으로 가장 양호한 것으로 나타났다. 질소원은 Y.E와 soytone등의 유기질소원 첨가가 효과적이었으나, 무기질소원의 효과는 나타나지 않았다. 무기염류 및 아미노산의 첨가는 대부분 효과적이었으며, 이 중 무기염류는$ MgSO_4$가 1.5배, 아미노산은 phenylalanine이 1.4배의 셀룰로오스 생산성을 나타냈다. 비타민은 $\alpha$-tocopherol의 첨가 시 1.4배의 생산성을 보였다. 또한 기본배지에 Coconut milk와 0.5%의 lignosulfonate의 첨가가 대조구와 비교하여 각각 2.2, 2.1배의 셀룰로오스의 생산성을 나타내어 실험 처리 중 가장 효과적이었다.

• 생약학회지
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• 제29권4호
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• pp.360-364
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• 1998

To develop new elicitors inducing the high productivity of taxane derivatives, plant growth inhibitors, namely, maleic acid hydrazide, N-phosphomethyl glycine and succinic acid 2.2-dimethyl hydrazide, coconut milk and yeast extract were administrated in the cell suspension culture system of Taxus cuspidata, and the production of baccatin III were analysed. The effects of amino acid related with the biosynthesis of baccatin III were also examined in these culture system. As the results, a remarkable enhancement of baccatin III production was observed in the cultivation with coconut water and with maleic acid hydrazide.

• 한국축산식품학회지
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• 제20권2호
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• pp.101-106
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• 2000

유지방구로부터 분리 정제한 lipase의 활성과 안정성에 미치는 pH의 영향을 야자유와 균질유를 기질로 하여 조사하였다. 효소원으로 buttermilk를 사용하였을 경우는 반응온도 $37^{\circ}C$에서 야자유, 균질유 모두 pH 9.5에서 최대 활성을 나타내었으며, 전형적인 종형의 pH의 존곡선을 나타내었다. 그러나 반응온도 $0^{\circ}C$에서는 pH 10.0까지 활성이 증가하였다. 정제 lipase를 사용한 경우는 반응응도 $37^{\circ}C$에서 균질유를 기질로 사용하였을 때 종 모양의 곡선을 나타내었으며, 최대 활성은 pH 9.0에서 나타났다. 반응온도 $0^{\circ}C$에서는 pH 10.0까지 활성이 계속 증가하였다. BSA를 첨가한 야자유를 기질로 사용한 경우는 반응온도 $37^{\circ}C$, $0^{\circ}C$ 모두 최대 활성이 pH 9.5에 나타났으며, pH 10.0에서는 활성이 현저하게 저하하였다. 정제 lipase의 안정성에 미치는 pH의 증가에 따라 현저하게 저하하였다. pH 10.0에서 $37^{\circ}C$로 20분간 효소를 유지하였을 경우 lipase의 활성은 pH 8.5 때의 활성에 비하여 13%로 감소하였다. 그러나, $4^{\circ}C$에서 60분간 효소를 유지하였을 경우는 lipase 활성이 pH 7.5∼10.0의 범위에서 안정하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제5권1호
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• pp.97-100
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• 1992

Locally available milk mix, coconut meal, cassava root meal, cassava leaf meal, maize, meat meal, fish meal, rice bran, rice polishings, wallow, molasses and coral sand in Fiji were analysed for proximate components, gross energy and mineral contents. The results obtained indicated typical and variable amounts of nutrients in these materials. On the basis of their nutrient contents, the potential usefulness and weaknesses of the various feed materials are discussed with specific reference to the formulations of diets for livestock and poultry in Fiji based on these locally available feed materials.