• 대한전기학회논문지:전기물성ㆍ응용부문C
• /
• 제54권8호
• /
• pp.378-383
• /
• 2005

We report the development of miniature fluorescence detection systems that employ miniature prism, mirrors and low coat CCD camera to detect the fluorescence emitted from 40 fluorescently-labeled protein patterns without scanner. This kind of miniature fluorescence detection system can be used in point of care. We introduce two systems, one uses prism+mirror block and the other uses prism and two mirrors. A large NA microscope eyepiece and low cost CCD camera are used. We fabricated protein chip containing multi-pattern BSA labeled with Cy5, using MEMS technology and modified the surface chemically to clean and to immobilize proteins. The measurements show that the combination of prism and mirrors can homogenize elliptical excitation light over the sample with higher optical efficiency, and increase the separation between excitation and fluorescence light at the CCD to give higher signal intensity and higher signal to noise ratio. The measurements also show that protein concentrations ranging from 10 ng/ml to 1000 ng/ml can be assayed with very small error. We believe that the proposed fluorescence detection system can be refined to build a commercially valuable hand-held or miniature detection device.

• 한국의상디자인학회지
• /
• 제10권2호
• /
• pp.105-115
• /
• 2008

Men's outer wear, such as jackets and coats appeared since the 16th century. They have been changed from doublets, coats, justaucorps, tail-coats, frock coats and morning coats to contemporary men's suits. Since the early 17th century, sleeve patterns of men's outer jackets had been mostly two-pieces close fitted patterns. The purpose of this article is to compare sleeve patterns of various jackets, which appear in various historic costume books. Armhole measurements, sleeve head measurements, amounts of ease, armhole depths, armhole widths, crown heights, sleeve width and sleeve angle had been compared to find out the differences among patterns of different times and styles. Coming to the present, the difference between top sleeve and under sleeve became more obvious, and the sleeve angle became less curvy. Another interesting point is that there had been considerable amounts of ease, which is the difference between sleeve head measurements and corresponding armhole measurements. It is because the sleeve has been attached to the armhole not by seam but by string. It is believed that the present sleeves have more natural curve and shape compared to the past.

• Molecules and Cells
• /
• 제42권4호
• /
• pp.356-362
• /
• 2019

The binding of MS2 bacteriophage coat protein (MCP) to MS2 binding site (MBS) RNA stem-loop sequences has been widely used to label mRNA for live-cell imaging at single-molecule resolution. However, concerns have been raised recently from studies with budding yeast showing aberrant mRNA metabolism following the MS2-GFP labeling. To investigate the degradation pattern of MS2-GFP-labeled mRNA in mammalian cells and tissues, we used Northern blot analysis of ${\beta}$-actin mRNA extracted from the Actb-MBS knock-in and $MBS{\times}MCP$ hybrid mouse models. In the immortalized mouse embryonic cell lines and various organ tissues derived from the mouse models, we found no noticeable accumulation of decay products of ${\beta}$-actin mRNA compared with the wild-type mice. Our results suggest that accumulation of MBS RNA decay fragments does not always happen depending on the mRNA species and the model organisms used.

• 복식
• /
• 제29권
• /
• pp.5-22
• /
• 1996

the present thesis aims to contribute to the further development of the men's wear indus-try in Korea which now confronts the need to adjust itself more actively to the conditions of the coming age of internationalization and free trade. To achieve this this thesis suggests the trade should provide for the changing domestic needs on theone hand and refine its wares as high value-added products in compe-tition with those from the advanced countries in fashion industry on the other, The history of the 'suit' the standard men's attire dates back to 1850 when the 'Ditto suit' was introduced composed of coat waist coat vest and trousers to remain virtually unchanged till the end of the nineteenth cen-tury and the modern men's wear originated in Britain. In Korea since the introduction of western clothes from 1876 custom-made suits had been dominant until 1944 while western clothes earned popular currency between 1945 and 1964 and ready-made suits began to spread with their advantages recognized in terms of practicality convenience and functionality The next phase from 1965 to 1985 witnessed the establishment of a number of ready-made brands although custom-made suits stayed in their height of prosperity until 1975. The turn-ing point for the men's wear industry came in 1975 when high-calss ready-made brands be-gan to turn up with the arrival of large companies in the market matching the change in consumers' life style. The men's wear market went through further diversification and specialization dur-ing the years between 1985. and 1990. Around the year 1990 however the expansion of the business suit industry came to slow down ac-companied by an oversupplied market. The fashion of pragmatism in the 1990s called for the growth of the casual wear department and as consumers' life style became more and more individualized conscious dressing in accord ance with T.P.O established itself as an important branch of culture, Such casual boom is likely to aid in promoting Korea's men's wear indus-try to peer with that of advanced countries. Consumers' fashion sense is now ahead of the trade's as well as being shortcycled highly individualized stylized and diversified. To meet consumers' demands under such circum-stances each company is required to develop its unique soft Know How based on accurate information and strategically specialized plan-ning. The trade should convert its hithero producer-oriented strategy to a new consumer oriented one and actively lead the needs and purchasin g pattern of consumers by providing an efficient and reasonable price policy with optimum supply of merchandise and also by presenting 'our' trend suited to our circum-stances.

• 식물병연구
• /
• 제7권1호
• /
• pp.1-7
• /
• 2001

1998년 수원 근교에서 채집한 전형적인 모자이크 병징을 나타내는 수국(Hydragea macrophylla for. otaksa)으로부터 CMV를 분리하고, Hm-CMV라 명명하였다. 기주실험, 물리적 실험, 혈청학적 성질, RNA와 coat protein의 성질, RT-PCR 및 RAP-PCR 분석을 통하여 Hm-CMV의 특성을 분석하였다. 12종의 CMV 지표식물에서 실시한 기주반응실험의 결과, 지금까지 보고된 CMV 계통들의 반응과 특징적인 차이는 인정되지 않았다. Hm-CMV의 물리적 성질은 내열성에서 6$0^{\circ}C$를 보여 기존 CMV들 보다 낮았다. 혈청학적으로 Hm-CMV는 Y-CMV와 융합하는 subgroup I CMV로 분석되었다. SDS-PAGE로부터에 Hm-CMV의 외피단백질은 28 kDa의 band가 확인되었으며, 4종의 게놈 RNA는 Y-CMV와 같은 분자량을 나타냈으나, 위성 RNA는 존재하지 않았다. 수국의 이병엽에서 분리한 dsRNA의 분석 결과도 Y-CMV와 같은 패턴을 보였다. Hm-CMV의 외피단백질유전자에 대한 RT-PCR 분석 결과, 예상된 분자크기의 DNA 증폭이 인정되었으며, PCR 산물을 이용한 EcoR I 및 Msp I을 처리한 결과는 subgroup I CMV의 특성을 나타냈다. 그런, RAP-PCR의 결과, Hm-CMV는 subgroup I내의 다른 계통들과 구분되었다.

• 농업과학연구
• /
• 제12권1호
• /
• pp.47-54
• /
• 1985

본(本) 조사연구(調査硏究)는 충남도내(忠南道內) 36개지역(個地域)에서 수집(蒐集)한 1984년도산(年度産) 메밀종자(種子)에 대(對)하여 몇가지 기초적(基礎的)인 특성(特性)을 조사분석(調査分析)하고 그들의 지역적분포(地域的分布)를 밝히고자 실시(實施)하였던 바 그 결과(結果)를 요약(要約)하연 다음과 같다. 1. 종자(種子)의 입형(粒型)은 유시형(有翅型), 중간형(中間型) 및 보통형(普通型)등 3개형(個型)이었고 어느 지성(地城)에서 수집(蒐集)한 종자(種子)이든 이들 3개형(個型)의 종자(種子)가 섞여 있었는데 그 혼합비율(混合比率)은 전체(全體) 평균(平均)으로 볼 때 보통형(普通型)이 50.1%, 중간형(中間型)이 26.8% 그러고 유시형(有翅型)은 23.1%이었으나 이러한 혼합비율(混合比率)은 지역간(地域間)에 큰 차이(差異)가 있었다. 2. 1000 입중(粒重)은 전체평균(全體平均) 24.9gr이었으나 지역간(地域間)에 큰 차이(差異)가 인정(認定)되어서 서산군(瑞山郡)((No.30)에서의 수집종자(蒐集種子)가 17.4gr로 가장 가벼웠고 서천군(舒川郡)(No.16)에서의 수집종자(蒐集種子)는 31.9gr로 가장 무거웠는데 입형별(粒型別) 혼합비율(混合比率)과 1000입중(粒重)과는 일정(一定)한 경향(傾向)이 나타나지 않았다. 3. $5^{\circ}C$로부터 $30^{\circ}C$사이에서는 온도(溫度)가 높아짐에 따라서 일반적(一般的)으로 발아(發芽)가 촉진(促進)되었는데 서천군(舒川郡)(No.16), 홍성군(洪城郡)(No.23), 천원군(天原郡)(No.35) 및 예산군(禮山郡)(No.27)에서의 수집종(蒐集種)은 $15^{\circ}C$ 그리고 서산군(瑞山郡)(No.29), 당진군(唐津郡)(No.32) 및 청양군(靑陽郡)(No.21)에서의 수집종(蒐集種)은 $25^{\circ}C$에서 발아율(發芽率)이 낮았다가 그 이상(以上)의 온도(溫度)에서 다시 상승(上昇)하였으며 $5^{\circ}C$의 저온(低溫)에서는 대부분(大部分)의 수집종자(蒐集種子)들이 10%미만(未滿)의 발아율(發芽率)을 나타내었으나 아산군(牙山郡)(No.33)과 대덕군(大德郡)(No.3)에서의 수집종(蒐集種)은 각각(各各) 20%와 30%의 발아율(發芽率)을 나타내었다. 4. 종피(種皮)의 색(色)은 흑색(黑色)과 암갈색(暗褐色)으로 구분(區分)되었으며 입형(粒型)과 종피색(種皮色)을 조합(組合)한 종자형(種子型)은 유시흑색(有翅黑色), 중간흑색(中間黑色), 보통흑색(普通黑色), 유시암갈색(有翅暗褐色), 중간암갈색(中間暗褐色) 그리고 보통암갈색종(普通暗褐色種) 등 6개형(個型)으로 분류(分類)할 수 있었고 종피색(種皮色)의 차이(差異)에 따른 광(光)Energy의 흡수(吸收) Spectrum의 차이는 190~390nm범위 이었다.

• 한국임상수의학회지
• /
• 제31권3호
• /
• pp.233-236
• /
• 2014

피부염 치료를 위해 스테로이드를 처방받은 경력이 있는 11년령 수컷 시추가 비만, 얇은 피모, 복부 팽만으로 내원하였다. 방사선 사진상 폐야 전반에 기관지-간질패턴과 뚜렷한 흉막음영이 관찰되었으나 신체검사상 이와 관련한 증상은 보이지 않았다. 복부 초음파상 부신은 정상크기이며 간실질의 에코성 상승을 나타내었다. 컴퓨터 단층촬영상 폐 중앙부는 간유리음영을 나타내었으며 변연부는 중앙부에 비해 저감약성을 띄고, 고감약성 결절음영이 폐 실질전반에 산재되어 있었다. 영상진단학적 소견과 부신피질자극 호르몬 검사결과를 바탕으로 의인성 부신피질기능항진증과 속발성 폐 석회화로 진단하였다.

• 패션비즈니스
• /
• 제7권2호
• /
• pp.15-25
• /
• 2003

In this study on the Persiandress called Persiandress or Mediandress, its names, styles, and structures that are still subject to a great deal of controversy were investigated. In addition, the reason why the Persiandress was wrongly named as Kandys by the early researchers was also examined. The documents of reliefs on the Apadanastairs in Persepolis and the European papers concerning this themes were subject of this analysis. Such investigation and examination results are summarized as follows. There is still no agreed name describing the Persiandress, which is called "Persiandress" or "Mediandress". Further, there is no definite conclusion on whether the Persiandress was made in one piece or two pieces. The first person who insisted that the Persiandress was made in one piece is a German archaeologist, Ernst Herzfeld. He said that the Persiandress was made in one long tetragonal piece, had a hole in the middle for head, and had lots of pleats when it was tied with a belt. On the contrary, researchers who insisted that the Persiandress was made in two pieces stressed the sleeve pleats in four folds, which is one of the representative characteristics, and said that such four-fold sleeve pleats would not be formed when the Persiandress was made in one piece and worn by a belt. Consequently, they have conducted studies according to their insistence and the resultant pattern. Kandys, the Median's typical dress, is a coat worn over the jacket and trousers. Although it had sleeves, the Median did not use them. Indeed, Kandys was used as a kind of cloak. However, the wrong term, Kandys is still used in Korea. So, it is necessary to correct use of such wrong term.

• 한국의상디자인학회지
• /
• 제11권1호
• /
• pp.53-62
• /
• 2009

Through the result of the investigation on costumes in Bulgarian regions, the main factors for the formation of Bulgarian folk costume and its kinds and history have been found out so far as follows. Alhtough varying according to the district and climate, Bulgarian folk costumes have some general features determined by the material, the pattern, the application. For women, Bulgarian costume consists of a white shirt, a single or double apron unique depending on each region(Bruchnick), a basic dress called a tunic (Soukman), and an open-tunic typed coat (saya). The main factors for the formation of Bulgarian folk costume have been under the influence of natural and environmental features and historical streams resulting from its geographical location. Bulgaria is agriculture-oriented society based on a continental climate. Accordingly, as for the classification of costumes, body-fit clothes, such as shirts and jackets which developed in Europe and fit the body shape, have featured in Bulgaria. Besides, Bulgaria was under the rule of Turk for a long time at the end of the Middle Age. Having been influenced a lot those days, its folk costume shows Turkish elements now. With geographical features, it was found that the southern area was influenced most by Turkey and Greece, and the types of folk costumes in Europe developed mainly from the northern area. The adaptation of traditional costume forms to the new cultural and progressive principles of appeal nowadays needs knowledge, as well as feeling. Finding the right measure and proportions of using old ethnic elemints in contemporary clothing is the prerequisite of successful design.

• 한국의류산업학회지
• /
• 제12권5호
• /
• pp.564-573
• /
• 2010

The purpose of this study is to investigate decoration techniques applied to the Korean traditional costume of modern style which are shown in special monthly magazines relevant to the traditional magazine. Findings of the study can be summarized as follows. The types of decoration techniques applied to the Korean traditional costume are largely classified into single and composite types. In the traditional costume of the former type, a quite many of gold and silver foils and embroideries are found. While, in that of the latter type, a combination of two different decorations, embroideries and seonchigi or multi-colored stripes and seonchigi is often used. These decoration techniques are usually applied to some parts of the costume like git, kkeutdong or cuffs, somae or sleeves, seop, goreum or coat strings, jacket or jeogori, the lower hem of the skirt and between joined parts in the skirt. Used together with the techniques, patterns are usually applied onto embroideries and gold or silver foils. Most of the patterns are flower in shape. The main material of the Korean traditional costume is fabrics that have no pattern on the surface and are commercially available irrespective of season. Git used for jeogori is largely classified into dangko git and donggrae git in type. Its color is in most cases same as the main color of jeogori. Keutdong for jeogori is usually 4 to 5.5 cm in breadth, classified as type II. Its color is in most cases white. Goreum for jeogori is usually large in length. Its color is most cases same as that of jegori or reddish purple or blackish red purple.