• Title/Summary/Keyword: co-transformation

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A Thermal Study of the Harmful Chemical Species of Charcoal and Their Transformation during Combustion (숯의 유해물질과 이들의 연소 중 상변화에 대한 열분석 연구)

  • Yoon, Hye-On;Kim, Ki-Hyun
    • Journal of the Mineralogical Society of Korea
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    • v.24 no.2
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    • pp.101-110
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    • 2011
  • Charcoal burning in the process of manufacture and ordinary use often release many constituent chemical species. As a result of open burning, the chemical composition as well as the physical properties of original material changes through the modification of surface properties of charcoal. Surface modification could be more responsible toward the outside elements for surface adsorption, it becomes easy to adsorb more toxic elements through surface adsorption. In this study, four kinds of commercially available charcoal were studied against the chemical and thermal stability along with the heavy metals and organic hazardous substances. Thermo gravimetric analysis (TGA) and differential scanning calorimetry, from room temperature to $400^{\circ}C$, were performed to study the weight loss and the changes in the behavior of those substances. According to TGA analysis, about 10% of weight loss was happened before $200^{\circ}C$. It was found that related weight loss of this temperature region may responsible to the gas phase organic matter. Natural charcoal, K1 and C1 show 15% of loss during the reaction heated to $400^{\circ}C$, while the artificial charcoal K2, C2 show the weight loss of about 20% was found. This is consistent with the main organic matter and VOC analysis results shown. Chemical composition based on the x-ray diffraction analysis was carried out. X-ray diffraction analysis reveals the existence of chemical additive in the forms of $Ba(NO_3)_2$, $BaCO_3$, and $NaNO_3$.

Selection of Herbicide Resistant Potatoes Transformed with Phosphinothricin Acetyltransferase Gene (Phosphinothricin Acetyltransferase 유전자 도입에 의한 제초제 저항성 감자의 선발)

  • Han, S.S.;Jeong, J.H.;Bang, K.S.;Yang, D.C.
    • Korean Journal of Weed Science
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    • v.17 no.4
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    • pp.390-399
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    • 1997
  • This experiment was conducted to introduce PAT (phosphinothricin acetyltransferase, non-selective herbicide bialaphos resistant gene) gene into potato (Solanum tuberosum. cv. Desiree). Optimal shoot regeneration from leaf discs and stem segments was obtained in MS medium supplemented with 0.1 mg/L IBA and 0.5 mg/L BA, and the frequency of shoot regeneration was 54% in left discs and 46% in stem segments. In this condition, leaf discs and stem segments of potato were co-cultivated with A. tumefaciens MP90 which contained binary vector with GUS: :NPTII gene and PAT gene. Transgenic shoots were regenerated from leaf and stem-derived calli on selection medium with 100mg/L kanamycin. The 100${\mu}M$ acetosyringone treatment during the co-cultivation highly enhanced(4 times than the control) the shoot regeneration on selection medium. When the putative transgenic plants were transferred to medium with 10mg/L basta, all of them were survived. After PCR. GUS test, and Southern blot analysis of the survived plant, we confirmed that the gene was stably integrated into the potato genome and expressed. After the transgenic plants were transplanted in soil, and the transgenic plants were sprayed with the herbicide basta (300ml/10a), the transgenic plants remained green but control plants were died.

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Characteristics of accumulated soil carbon and soil respiration in temperate deciduous forest and alpine pastureland

  • Jeong, Seok-Hee;Eom, Ji-Young;Park, Ju-Yeon;Lee, Jae-Ho;Lee, Jae-Seok
    • Journal of Ecology and Environment
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    • v.42 no.1
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    • pp.20-29
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    • 2018
  • Background: For various reasons such as agricultural and economical purposes, land-use changes are rapidly increasing not only in Korea but also in the world, leading to shifts in the characteristics of local carbon cycle. Therefore, in order to understand the large-scale ecosystem carbon cycle, it is necessary first to understand vegetation on this local scale. As a result, it is essential to comprehend change of the carbon balance attributed by the land-use changes. In this study, we attempt to understand accumulated soil carbon (ASC) and soil respiration (Rs) related to carbon cycle in two ecosystems, artificially turned forest into pastureland from forest and a native deciduous temperate forest, resulted from different land-use in the same area. Results: Rs were shown typical seasonal changes in the alpine pastureland (AP) and temperate deciduous forest (TDF). The annual average Rs was $160.5mg\;CO_2\;m^{-2}h^{-1}$ in the AP, but it was $405.1mg\;CO_2\;m^{-2}h^{-1}$ in the TDF, indicating that the Rs in the AP was lower about 54% than that in the TDF. Also, ASC in the AP was $124.49Mg\;C\;ha^{-1}$ from litter layer to 30-cm soil depth. The ASC was about $88.9Mg\;C\;ha^{-1}$, and it was 71.5% of that of the AP. The temperature factors in the AP was high about $4^{\circ}C$ on average compared to the TDF. In AP, it was observed high amount of sunlight entering near the soil surface which is related to high soil temperature is due to low canopy structure. This tendency is due to the smaller emission of organic carbon that is accumulated in the soil, which means a higher ASC in the AP compared to the TDF. Conclusions: The artificial transformation of natural ecosystems into different ecosystems is proceeding widely in the world as well as Korea. The change in land-use type is caused to make the different characteristics of carbon cycle and storage in same region. For evaluating and predicting the carbon cycle in the vegetation modified by the human activity, it is necessary to understand the carbon cycle and storage characteristics of natural ecosystems and converted ecosystems. In this study, we studied the characteristics of ecosystem carbon cycle using different forms in the same region. The land-use changes from a TDF to AP leads to changes in dominant vegetation. Removal of canopy increased light and temperature conditions and slightly decreased SMC during the growing season. Also, land-use change led to an increase of ASC and decrease of Rs in AP. In terms of ecosystem carbon sequestration, AP showed a greater amount of carbon stored in the soil due to sustained supply of above-ground liters and lower degradation rate (soil respiration) than TDF in the high mountains. This shows that TDF and AP do not have much difference in terms of storage and circulation of carbon because the amount of carbon in the forest biomass is stored in the soil in the AP.

Comparison of Immunohistochemical Expression of CBP(cAMP-responsive Element Binding Protein) Transcriptional Co-activator between Premalignant Lesions and Squamous Cell Carcinomas in the Lungs (전암성 폐병변 및 편평상피세포폐암 조직에서 CBP(cAMP-responsive Ele-ment Binding Protein) 전사 공동 활성인자의 면역조직화학적 발현양상의 비교)

  • Shin, Jong Wook;Kim, Jin Soo;Kim, Mi Kyung
    • Tuberculosis and Respiratory Diseases
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    • v.63 no.2
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    • pp.165-172
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    • 2007
  • Background: The pathogenesis of lung cancer includes the accumulation of multiple genetic abnormalities. The CREB-binding protein(CBP) is one of several transcriptional co-activators among various sequence-specific DNA-binding transcription factors. CBP is involved in a wide range of cellular activities, such as DNA repair, cell growth, differentiation, and apoptosis that are suspected of contributing to tumorigenesis. The goal of this study was to evaluate CBP expression in a series of human lung tissues containing normal epithelium, premalignant lesions(hyperplasia and dysplasia) and squamous cell carcinomas. Materials and Methods: Immunohistochemical staining was performed on formalin-fixed paraffin-embedded sections by use of a monoclonal anti-CBP antibody. CBP expression was compared in samples from 120 patients with premalignant and malignant histological types including 20 metaplastic specimens, 40 dysplastic specimens, and 60 squamous cell carcinomas in the lung. Results: CBP expression was seen in 35% (7/20) of the metaplastic specimens. 65% (26/40) of the dysplastic specimens, and 70% (42/60) of the squamous cell carcinomas (p<0.05). According to celluar atypism, CBP expression was 50% (10/20) of the low-grade dysplastic specimens and 80% (16/20) of the high-grade dysplastic specimens(p <0.01). By cellular differentiation, CBP expression was seen in 95% (19/20) of the well differentiated squamous cell carcinomas, 85% (17/20) of the moderately differentiated carcinomas and 30% (6/20) of the poorly differentiated lesions (p <0.05). Conclusion: These results suggest that CBP may have an important role in malignant transformation of precancerous lung lesions and may be a marker for malignancy.

Removal of ZnO Nanoparticles in Aqueous Phase and Its Ecotoxicity Reduction (수계 내 ZnO 나노입자의 제거 및 생태독성 저감)

  • Kim, Hyunsang;Kim, Younghun;Kim, Younghee;Lee, Sangku
    • Clean Technology
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    • v.22 no.2
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    • pp.89-95
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    • 2016
  • The nanotoxicity of ZnO nanoparticles used in cosmetics and tire industry is one of emerged issues. Herein, the removal of ZnO nanoparticles dispersed in aqueous phase and its ecotoxicity were investigated. In the short-term exposure for fertilized eggs (O. latipes), the deformity was observed at 5 mg L−1 of ZnO nanoparticles in some individuals and delayed hatching of eggs by retarded growth was observed at 10 mg L−1 of ZnO nanoparticles. This result show that ZnO nanoparticles have cytotoxic effect to the organisms lived in water phase. Therefore, herein, the removal of ZnO nanoparticles in aqueous phase by chemical precipitation was investigated. After addition of Na2S and Na2HPO4, the precipitated ZnO was transformed to ZnS and Zn3(PO4)2 particles, respectively. The removal efficiency of ZnO was reached to almost 100% for two cases. In addition, the toxicity tests about ZnS and Zn3(PO4)2 particles showed no acute toxicity for D. magna. This implies that transformation of ZnO to ZnS and Zn3(PO4)2 particles with very low ionization constant might decrease effectively the toxicity of ZnO.

Isolation of Stenotrophomonas rhizopilae Strain GFC09 with Ginsenoside Converting Activity and Anti-wrinkle Effects of Converted Ginsenosides (사포닌 전환 활성 Stenotrophomonas rhizopilae Strain GFC09 균주의 분리 동정 및 전환 사포닌의 주름 개선 효과)

  • Min, Jin Woo;Kim, Hye-Jin;Joo, Kwang-Sik;Kang, Hee-Cheol
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.4
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    • pp.375-382
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    • 2015
  • Ginsenosides (ginseng saponin) as the one of important pharmaceutical compounds of ginseng and is responsible for the pharmacological and biological activities. These ginsenoside produces diverse small molecules ginsenoside which have more pharmacological activities including anti-wrinkle, anti-cancer and anti-oxidant effects. In the present study, we isolated bacteria using esculin agar, to produce ${\beta}$-glucosidase, and we focused on the bio-transformation of ginsenoside. Phylogenetic tree analysis was performed by comparing the 16S rRNA sequences; we identified the strain as Stenotrophomonas rhizopilae strain GFC09. In order to determine the optimal conditions for enzyme activity, the crude enzyme was incubated with 1 mM ginsenoside $Rb_1$. Bioconversion of ginsenoside $Rb_1$ were analyzed using TLC and HPLC. The crude enzyme hydrolyzed the ginsenoside $Rb_1$ along the following pathway: LB: $Rb_1{\rightarrow}Rd{\rightarrow}F_2$ into compound K, TSB: $Rb_1{\rightarrow}Rd{\rightarrow}F_2$. The structure of the hydrolyzed metabolites were identified by NMR. The activity screening tests showed that the conversion product induced the production of type I procollagen in a dose-dependent manner. These results suggested that hydrolyzed ginseng product containing the ginsenoside $F_2$ and compound K could be useful as an active ingredient for wrinkle-care cosmetics.

A Methodology of AI Learning Model Construction for Intelligent Coastal Surveillance (해안 경계 지능화를 위한 AI학습 모델 구축 방안)

  • Han, Changhee;Kim, Jong-Hwan;Cha, Jinho;Lee, Jongkwan;Jung, Yunyoung;Park, Jinseon;Kim, Youngtaek;Kim, Youngchan;Ha, Jeeseung;Lee, Kanguk;Kim, Yoonsung;Bang, Sungwan
    • Journal of Internet Computing and Services
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    • v.23 no.1
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    • pp.77-86
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    • 2022
  • The Republic of Korea is a country in which coastal surveillance is an imperative national task as it is surrounded by seas on three sides under the confrontation between South and North Korea. However, due to Defense Reform 2.0, the number of R/D (Radar) operating personnel has decreased, and the period of service has also been shortened. Moreover, there is always a possibility that a human error will occur. This paper presents specific guidelines for developing an AI learning model for the intelligent coastal surveillance system. We present a three-step strategy to realize the guidelines. The first stage is a typical stage of building an AI learning model, including data collection, storage, filtering, purification, and data transformation. In the second stage, R/D signal analysis is first performed. Subsequently, AI learning model development for classifying real and false images, coastal area analysis, and vulnerable area/time analysis are performed. In the final stage, validation, visualization, and demonstration of the AI learning model are performed. Through this research, the first achievement of making the existing weapon system intelligent by applying the application of AI technology was achieved.

A Content Analysis of B-Class Emotional Advertising Trend: Focused on TV commercials from 2015 to 2020 (B급 감성 광고 경향에 관한 내용분석: 2015년부터 2020년까지 공중파 TV광고를 중심으로)

  • Baik, Juyoun;Youm, Dongsup
    • Journal of the Korea Convergence Society
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    • v.13 no.1
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    • pp.179-188
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    • 2022
  • This study discovers the general characteristics of B-class Emotional Advertisements and analyses their trend. A content analysis was conducted on 498 advertisements on-aired between 2015 and 2020, which were sampled from the advertisements registered in the TVCF(www.tvcf.co.kr), the largest advertisement web portal in the Republic of Korea. The analysis concludes that the B-class Emotional Advertisements, employed in a wide range of genre, is most incorporated in comedy/exaggeration genres and is on a rising trend due to 2020 COVID-19 Pandemic. Furthermore, it is confirmed that the utilization of B-class emotional advertisement has also increased in domain of non-commercial advertisements, such as Public Service Advertisements, Governmental/Organizational Advertisements, and Corporate Public Relations (PR) Advertisements. The study validates the transformation of the B-class emotional advertisements from a demonstration of an eccentric minority subculture to an epitome of a new and adventurous mainstream culture, successfully serving a central role in both the commercial and non-commercial sectors. Depicting the caricatures of the social, cultural and economic phenomenon and the recent surge of individual's depression, fatigue and pessimism, B-class emotional advertisements provide sympathetic and emotional alleviating ground for people that contributed to its rise.

The Conceptual Intersection between the Old and the New and the Transformation of the Traditional Knowledge System (신구(新舊) 관념의 교차와 전통 지식 체계의 변용)

  • Lee, Haenghoon
    • The Journal of Korean Philosophical History
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    • no.32
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    • pp.215-249
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    • 2011
  • This essay reflects on the modernity of Korea by examining the transformation of the traditional knowledge system from a historico-semantic perspective with its focus on the opposition and collision of the old and the new conception occurred in the early period(1890~1910) of the acceptance of the Western modern civilization. With scientific success, trick of reason, Christianity and evolutionary view of history, the Western modernity regarded itself as a peak of civilization and forced the non-Western societies into the world system in which they came to be considered as 'barbarism(野蠻)' or 'half-enlightened(半開).' The East Asian civilization, which had its own history for several centuries, became degraded as kind of delusion and old-fashioned customs from which it ought to free itself. The Western civilization presented itself as exemplary future which East Asian people should achieve, while East Asian past traditions came to be conceived as just unnecessary vestiges which it was better to wipe out. It can be said that East Asian modernization was established through the propagation and acceptance of the modern products of the Western civilization rather than through the preservation of its past experience and pursuit of the new at the same time. Accordingly, it is difficult to apply directly to East Asian societies Koselleck's hypothesis; while mapping out his Basic Concept of History, he assumed that, in the so-called 'age of saddle,' semantic struggle over concepts becomes active between the past experience and the horizon of expectation on the future, and concepts undergoes 'temporalization', 'democratization', 'ideologization', 'politicization.'The struggle over the old and new conceptions in Korea was most noticeable in the opposition of the Neo-Confucian scholars of Hwangseongsinmun and the theorists of civilization of Doknipsinmun. The opposition and struggle demanded the change of understanding in every field, but there was difference of opinion over the conception of the past traditional knowledge system. For the theorists of civilization, 'the old(舊)' was not just 'past' and 'old-fashioned' things, but rather an obstacle to the building of new civilization. On the other hand, it contained the possibility of regeneration(新) for the Neo-Confucian scholars; that is, they suggested finding a guide into tomorrow by taking lessons from the past. The traditional knowledge system lost their holy status of learning(聖學) in the process of its change into a 'new learning(新學),' and religion and religious tradition also weakened. The traditional knowledge system could change itself into modern learning by accepting scientific methodology which pursues objectivity and rationality. This transformation of the traditional knowledge system and 'the formation of the new learning from the old learning' was accompanied by the intersection between the old and new conceptions. It is necessary to pay attention to the role played by the concept of Sil(hak)(實學) or Practical Learning in the intersection of the old and new conceptions. Various modern media published before and after the 20th century show clearly the multi-layered development of the old and new conceptions, and it is noticeable that 'Sil(hak)' as conceptual frame of reference contributed to the transformation of the traditional knowledge system into the new learning. Although Silhak often designated, or was even considered equivalent to, the Western learning, Neo-Confucian scholars reinterpreted the concept of 'Silhak' which the theorists of civilization had monopolized until then, and opened the way to change the traditional knowledge system into the new learning. They re-appropriated the concept of Silhak, and enabled it to be invested with values, which were losing their own status due to the overwhelming scientific technology. With Japanese occupation of Korea by force, the attempt to transform the traditional knowledge system independently was obliged to reach its own limit, but its theory of 'making new learning from old one' can be considered to get over both the contradiction of Dondoseogi(東道西器: principle of preserving Eastern philosophy while accepting Western technology) and the de-subjectivity of the theory of civilization. While developing its own logic, the theory of Dongdoseogi was compelled to bring in the contradiction of considering the indivisible(道and 器) as divisible, though it tried to cope with the reality where the principle of morality and that of competition were opposed each other and the ideologies of 'evolution' and 'progress' prevailed. On the other hand, the theory of civilization was not free from the criticism that it brought about a crack in subjectivity due to its internalization of the West, cutting itself off from the traditional knowledge system.

The Influence of Pretreatment Period, 2-Hydroxynicotinic Acid and Anther Co-pretreatment on Embryo Induction in Isolated Microspore Culture of Capsicum annuum L. (고추의 나출 소포자 배양시 전처리 기간, 2-Hydroxynicotinic Acid 및 약-공동전처리가 소포자배 발생에 미치는 영향)

  • Park Eun-Joon;Kim Jin-Ae;Lee Jong-Suk;Jang In-Chang;Yoon Michung;Chung Sang-Ho;Kim Moonza
    • Journal of Plant Biotechnology
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    • v.32 no.1
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    • pp.37-44
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    • 2005
  • Microspores were isolated from pepper (Capsicum annuum L.) anthers by using a micro-blender and cultured in modified NLN medium at $25^{\circ}C$. The influence of pretreatment period at $32^{\circ}C$, adding the 2-hydroxynicotinic acid to a pretreatment medium, and co-pretreatment anthers with microscopes on the induction of embryo were examined. Globular and torpedo embryos were observed from 3 weeks after culture. Embryo development was not synchronized within culture. After 4 weeks in culture, in addition to globular and torpedo embryos, cotyledonary embryos were observed. Normal cotylodonary embryos developed into plantlets when transferred to a solid hormone free B5 medium containing $2\%$ sucrose. Embryo yields were significantly higher after 1- and 2-day pretreatment at $32^{\circ}C$. However the development of embryo ceased at the globular or heart stage. In contrast, embryo yields were lower after 3- to 6-day pretreatment at $32^{\circ}C$ and embryo developed at the cotyledonary stage. After adding the 2-hydroxynicotinic acid to anther pretreatment solution, embryo yields were slightly increased. However most embryos occurred were at the globular or heart stage. Co-pretreatment of microspores with anthers was deleterious for embryo induction and development. AS far as we know, this is the first report of success in obtaining high frequency of embryogenesis and plantlets formation from isolated microspores of pepper. Although the culture conditions have to be optimized further, this promising microspore culture system can be used for genetic transformation, selection for dominant and recessive traits as well as for the production of homozygous doubled haploid plants.