• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2003.04a
• /
• pp.132-132
• /
• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2002.04b
• /
• pp.152-152
• /
• 2002

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.11a
• /
• pp.184-184
• /
• 2005

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.04a
• /
• pp.36-36
• /
• 2018

Soybean is the important crop in Asian countries as protein source, oil production and animal feed. Improving soybean using genetic transformation is the principal tool in nowadays. Developing herbicide resistant transgenic soybean plants through Agrobacterium-mediated transformation has been worked in many previous studied. However, the transformation efficiency is still low. Many attempts try to find the optimum media condition for plant regeneration after infection. After transformation, the plant regeneration is very important condition to promote growth of transgenic plant. In this study, we optimized a regeneration condition for two Korean soybean cultivar, Dawonkong and Pungsannamulkong using cotyledon, cotyledonary nodes and hypocotyl as explant. The results showed that shoot regeneration of cotyledonary nodes on B5 medium containing 2 mg/L 6-benzylaminopurine showed the highest percentage of regeneration in Dawonkong (75.8%) while Pungsannamulkong presented high number of shoots 2.12 shoots per explant. For transformation condition, co-cultivation in 7 days showed a high number of GUS positive expression. Most of explants can survived under media including 5 mg/L of glufocinate which refers phosphinotricin for 2-week selection. Washing with 400 mg/L of cefotaxime in several times and selection in plant regeneration media with 400 mg/L of cefotaxime can prevent bacteria growth, effectively.

• Korean Journal of Plant Resources
• /
• v.27 no.3
• /
• pp.242-248
• /
• 2014

This study was conducted to establish an efficient transformation system by using somatic embryogenesis in an important Korean native conifer, Korean fir (Abies koreana). Embryogenic masses were induced from mature zygotic embryos of the Korean fir on Schenk and Hildebrandt medium, which was supplemented with thidiazuron. For genetic transformation, the embryogenic masses were co-cultivated with a disarmed Agrobacterium tumefaciens strain C58/pMP90 containing the plasmid vector pBIV10 or LBA4404 containing the plasmid vector MP90. Both vectors contain the kanamycin resistance and beta-glucuronidase (GUS) reporter genes. A total of 48 lines of embryogenic masses were selected on mLV medium containing $50{\mu}g/mL$ of kanamycin after 4 weeks of culture, following 3 days of co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 (none of the lines was cultivated with strain LBA4404 carrying MP90). Quantitative real-time PCR was performed, and high levels of GUS transcripts were observed in the 48 putative transgenic lines; however, the control (non-transgenic line) showed negative results. Results of histochemical staining showed that the expression of the GUS reporter gene was observed in somatic embryos that developed from the embryogenic masses of all 48 lines. Stably transformed cultures were successfully produced by co-cultivation with A. tumefaciens strain C58/pMP90 carrying pBIV10 in Korean fir. Here, we have reported an Agrobacterium-mediated gene transfer protocol via somatic embryogenesis that may be helpful in developing breeding and conservation strategies for the Korean fir.

• Journal of The Korean Association For Science Education
• /
• v.24 no.1
• /
• pp.76-89
• /
• 2004

Recent studies of science and technology "in-the-making" revealed that the process of designing material artifacts is not a straightforward application of prior images or theories by one (or more) person(s) isolated from his or her (their) environment. Rather, designing is a process contingent on the social and material setting for both engineering designers and students. Over the past decade, designing technological artifacts has emerged as an important learning environment in science classrooms. Through the analyses of a large database concerning an innovative simple machines curriculum for sixth-and seventh-grade students, we accumulated valid evidence for the nature of the designing process and science learning through it. In this paper, we show that design actions intertwine with the transformation of the objectified raw materials and artifact, the designer collective, and the mediating tools enabling that transformation, which constitute the elements of an activity from the perspective of cultural-historical activity theory. We conceptualize the continuous change of relation between material artifacts, designers, and tools throughout the design activity as co-evolution. Two episodes were selected to exemplify synchronic and diachronic change of relations inherent in co-evolving activity system. Finally, we discuss the implications of co-evolution during design activity for science learning.

• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2005.04a
• /
• pp.131-131
• /
• 2005

• Bulletin of the Korean Chemical Society
• /
• v.15 no.12
• /
• pp.1129-1131
• /
• 1994

• Journal of the Korean Society for Heat Treatment
• /
• v.3 no.4
• /
• pp.1-9
• /
• 1990

In this study, the ausformed martensite cooled to $-196^{\circ}C$ with various deformation degrees in Fe-30%Ni-0.24%C alloy was transformed to reversed austenite at $500^{\circ}C$ by cyclic reverse martensitic transformation. The effects of prior deformation and the number of cyclic reverse transformation on the microstructure and the mechanical properities of reversed anstensite were investigated. Experimental results showed that the strength of reversed austenite was higher than that of original austenite. This is due to higher dislocation density and grain refining. The reversed austenite formed from ausformed martensite was highly strengthened by prior deformation. This strengthening effect of reversed austenite is attributed to higher dislocation density than grain fefining. The yield strength of reversed austenite below 30% prior deformation, but above 30% prior deformation the strength of reversed austenite is lower than that of deformed austenite. This is due to partly disappearance of strain hardening effect at higher deformation degree by reverse transformation. The strength of reversed austenite is increased with the number of cyclic transformation. Especially, it is principally strengthened by the first cyclic transformation and shows higher increase in yield strength than that of ultimate tensile strength.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.52-52
• /
• 2018

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.