• Applied Biological Chemistry
• /
• v.14 no.1
• /
• pp.35-41
• /
• 1971

Changes in fatty substance of hot pepper fruit during the after-ripening period were studied for both neutral and polar fatty substance. The results obtained from these studies are tabulated as follows; 1. Total fatty substance decreased as the after-ripening proceeded, while neutral fatty substance was least during the climacteric period. Polar fatty substance showed sudden drop in the amount during the post-climacteric period. 2. Fatty acid composition in neutral fatty substance showed that there is decrease in linoleic acid during the post-climacteric period, and in myristic acid and oleic acid respectively during the climacteric period. Stearic acid contents also drops sharply as after-ripening proceeds. 3. Value for saturates to unsaturates in and the amount of neutral fatty substance became least during the climacteric period, indicating that there is relationship between metabolism of neutral fats and climacteric rise. 4. Fatty acid composition of polar fatty substance showed that there is decrease in linoleic acid when after-ripening takes place whereas linolenic acid increases. These became reverse in the amounts after the climacteric period, suggesting that these changes may be useful to indicate true maturity of the fruits.

• Applied Biological Chemistry
• /
• v.23 no.1
• /
• pp.31-44
• /
• 1980

Climacteric phenomon in fruits was delayed ten days on the sub-atmospheric treatment than the normal atmospheric one. Average lipid content in tomatoes sampled was composed of neutral lipid (49%), phospholipid(31%) and glycolipid (13%). Seven constituents each from neutral and phospholipid were seperated through thin-lyaer chromatography. Changes on lipid content during treatments were associated with the climacteric pattern and the advance of maturity; At the climacteric on-set, the fruit color was light pink, and the content of neutral lipid was reduced to minimum. After this stage, this content was reversely increased. The maturity was related with diglyceride and sterol ester in neutral lipid, and phosphatidyl choline and phosphatidyl ethanolamine in phospholipid. The changes of two constituents each on both neutral and phospholipid were reversed each other. It was concluded that changes of lipid content in tomato fruits were closely associated with the advance of fruit maturity and climacteric pattern.

• Korean Journal of Agricultural Science
• /
• v.29 no.1
• /
• pp.1-7
• /
• 2002

Postharvest characteristics of 'Pingguoli' fruit produced between China and Korea were compared to find an ecological difference. There was a difference in fresh weight per fruit, thus, fruit weight produced in Korea was much higher than those in China. However, no significant differences were confirmed in the level of soluble solid content and flesh firmness. The acidity of Korean 'Pingguoli' was higher than Chinese ones. The external appearance of Korean 'Pingguoli' was not attractive due to the rough and rugged surface. Thus, the prospect of 'Pingguoli' cultivation in Korea seems not to be promised. The general trend of respiration and ethylene evolution during storage did not significantly differed but the amount of $CO_2$ and ethylene production was higher in fruits produced in China. There was a strong similarity in respiration and ethylene evolution pattern to typical climacteric fruits. Thus, 'Pingguoli' is considered as a climacteric fruit. Even though the rate of ethylene production of 'Pingguoli' was relatively high, the storability of 'Pingguoli' showed a strong potential. The further studies in postharvest physiology of pear using 'Pingguoli' and in its breeding potentials as a source of long storage life are needed.

• Applied Biological Chemistry
• /
• v.28 no.3
• /
• pp.174-181
• /
• 1985

Ultrastructural changes in the chloroplast of apple fruits was examined with Malus Pumila Mill var. Fugi harvested during preclimacteric stage the subatmospheric pressure storage condition at 380mmHg, $20^{\circ}C$. Electron micrographs obtained from the fruits stored at 760mmHg, $20^{\circ}C$ indicated that the initiation of ultrastructural changes is in parallel with the onset of respiratory climacteric. Chloroplast of the apple fruits stored for 25 days is vacuolated and the lamellar system are a little disorganized. Chloroplast of the apple fruits stored for 50 days is showing extensive vacuolation, the lamellar system are completely disorganized and the plasma membrane has pulled away from the cell wall. In the case of subatmospheric presssure storage caused to delay the onset of ultrastructural changes in chloroplast such as vacuolation, disorganization of lamellar system and dispersal of stroma lamella about 2 months. These results suggested that ripening of apple fruits is correlated not only to the activity of respiration but to the involvement of ultrastructural changes in fruit cells. The evidences obtained here led to the conclusion that subatmospheric pressure treatment extends storage life of apple fruits by inhibiting ultrastructural changes of cell organelles in concomitance with respiratory activity.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.19 no.2
• /
• pp.115-120
• /
• 1990

This study was conducted to investigate the relation of softening with the mineral compone-nts during ripening of tomato fruits. The mineral contents of Ca, K, Mg, Zn. Fe Mn and Cu and its existence form respiratory rate and hardness during ripening were evaluated. The respiratory rate of tomato fruits was decreased until 42 days after anthesis and then increased the climacteric maximum was found on 49 days after anthesis then decreased. The hardness of tomato fruits during ripening did not change greatly until 45days after anthesis then decrea-sed markedly. The major mineral components of tomato fruits were K, Ca, and Mg, Zn Fe Mn and Cu were the minor components. The contents of soluble Ca, Ma and K increased markedly and those of bound Ca and Mg decreased markedly after 45days during ripening,. However other components were not changed greatly.

• Applied Biological Chemistry
• /
• v.19 no.4
• /
• pp.202-218
• /
• 1976

In this experiment American Summer Pairman apple was selected as test fruit in order to study the effect of sub-atmospheric pressure storage in the apple. keeping a certain pressure condition, a new sub-atmospheric pressure system was designed and constructed in which fruits were stored at $25^{\circ}C$ under two different atmospheric pressure conditions such as Normal Atmospheric Pressure (NAP) and Sub-Atmospheric Pressure (SAP). Moreover, they were divided into plots of 5% and 0% of $CO_2$, on the basis of gas composition. Under these conditions, the amount of respiration and ethylene evolution, and the changes of intercellular gas composition and organic components were investigated throughout the storage. The results obtained are as follows: (1) The intercellular gas was exhausted so rapidly by the SAP treatment that the gas equilibrium in the tissues reached within 5 minutes. (2) The amount of respiration was found to be higher in plots of NAP than SAP, and under the conditions, controlling $CO_2$ content, plots of $CO_2$ 5% were lower in the amount of respiration than $CO_2$ 0%. The climateric rise was revealed more slowly in plots of the SAP than NAP. These results indicate that the SAP treatment was an efficient method for controlling the respiration of fruits. Furthermore, these results were also realized with the results of the respiratory quotient (R.Q) and intercellular gas composition. (3) Evolution of ethylene, the ripening hormone in plant, was shown the similar tendency to the climacteric pattern of respiration; at the stage of climacteric maximum, the maximun amount of ethylene was found earlier in plots of NAP than SAP, and post climacteric stage was prolonged in the plots of the SAP compared to those of the NAP. The ethylene concentration in tissue appeared lower in plots of the SAP than NAP, which might suggest that the SAP treatment was caused to restriction of ethylene evolution. (4) Effects of the SAP treatment mentioned hitherto were proved also with the test of the external appearance such as changes of color and freshness, firmness, rotting and weight loss. (5) In the investigation of organic components, vitamin C and organic acids varied less in plots of SAP than NAP. Specially, it was remarkable that the loss of malic acid was least decreased by the SAP treatment. These effects of the SAP treatment were distinctive in the changes of the ratios of malic and citric acid to total acid, and the ratios of free sugar to free acid.

• Food Science and Preservation
• /
• v.12 no.5
• /
• pp.424-431
• /
• 2005

'Hongro' and 'Gamhong' apples grown at the Gochang-si in the southern part of Korea were harvested on September in 2002 for storage study. Each cultivar was divided into two groups of maturity based on the harvested date. Immature fruits were harvested 10 days early compared to fruits having proper maturity. This study was conducted to evaluate quality the change of 'Hongro' and 'Gamhong' apples newly bred by different storage methods with PE film package and CA conditions. A typical climacteric respiration pattern was observed in new variety 'Hongro' and 'Gamhong' apples stored at room temperature. The results indicated that fresh quality of the stored fruits in CA condition was better than that in other treatments during storage. Qualities of apples were least changed at CA storage of 3% $CO_2$ and 1% $O_2$. CA storage was effective in maintaining the freshness of 'Hongro' and 'Gamhong' for 16 weeks.

• Journal of Crop Science and Biotechnology
• /
• v.10 no.3
• /
• pp.167-174
• /
• 2007

Spongy Alphonso mangoes were found to be infected with Staphylococcus bacteria. A Gram positive Staphylococcus strain was isolated from spongy pulp and identified from CABI Bioscience, UK, by partial 16S rDNA sequence analysis and by morphological and biochemical characterization through IMTECH, Chandigarh, India. Although identification by both of these methods indicated the organism belonged to same genus, different species names were given. Changes in total phenolics, reducing, and non-reducing sugars, respiration rate, total carotenoids, peroxidase(POX), and catalase activities were monitored during ripening of these fruits. The climacteric rise in spongy fruits was marked by an increase in respiration rate and a decrease in sugar content. Total phenolics content increased in spongy fruits as compared to ripe non-spongy fruits. Development of corky white tissue in spongy fruits was associated with about a 2.5-fold reduction in total carotenoids and a concomitant increase in lipoxygenase-mediated, $\beta$-carotene co-oxidation. A marked decrease in soluble protein content and about a 1.5-fold increase in POX activity was observed. Maximum POX activity was confined to 50-70%$(NH_4)_2SO_4$ fraction. The intense dark bands visible after POX specific substrate staining of the Native gel indicated a high expression of isoenzymes of POX in spongy fruits. Similarly, changes in levels of catalase activity were also observed in spongy fruits. The results suggest that infection of Alphonso mangoes with Staphylococcus bacteria affects the normal ripening processes of the fruit interfering with the carbohydrate and carotenoid metabolism. Also, the studies indicate the expression of POX and catalase enzymes as a plant defense response to microbial invasion.

• Horticultural Science & Technology
• /
• v.34 no.5
• /
• pp.746-754
• /
• 2016

This study was conducted to assess the effect of ethylene producing tablet (patent no.10-1574011) and treatment period on quality and storability of banana (AAA - Cavendish group) fruit imported into Korea from the Philippines. Three and five days of treatment periods, and different concentrations of ethylene tablet (50 ppmv, 100 ppmv) and control (standard ethylene gas treatment at a concentration of 100 ppmv) were used for the study. Slightly higher respiration rate was observed in banana fruits treated with 50 ppmv in both 3 and 5 days after treatments as compared to the control. Better storability was observed with banana fruits treated with 50 ppmv and 100 ppmv as compared to the control. All the treatments were shown a decrease in firmness as the storage days proceeded. The highest firmness was recorded from 50 ppmv on the initial day of storage while the lowest was recorded from 100 ppmv on 9 day storage. It was observed that banana fruits could be stored for up to 12 days without losing their color quality in all treatments except for the control. However, the quality of fruits at the control rapidly decreased (lost marketability) after 9 days of storage. Banana fruits treated with ethylene tablet with 100 ppmv for 5 days recorded the highest soluble solids content (SSC) at the beginning of storage period which was similar with the control. However, banana fruits treated with ethylene tablet with 100 ppmv for 3 days showed better storability than the control. On the other hand, ethylene tablet with 50 ppmv for 5 days of treatment has extended banana shelf life without affecting peel color, firmness and SSC content. Hence, these results indicate that banana fruits treated with ethylene tablet with 100 ppmv for 3 days or /and 50 ppmv for 5 days are an optimum for ripening of banana to be used for market and ultimate consumption.

• Microbiology and Biotechnology Letters
• /
• v.1 no.1
• /
• pp.25-30
• /
• 1973

Experiments were carried out to clarify changes of intercellular gas composition, respiration and ethylene evolution by controlled external atmospheric pressure and external gas composition on apples. Jonathan apples, harvested 25, in September and Rolls apples, harvested 25, in October, 1971 and 1972 were used. Results obtained were as follows: 1. Intercellular gas volumes were proportionally changed by the given pressure, and the given pressure remarkably affected internal gas composition and volume of apples. As intercellular gas volumes were reached in their equilibrium within 5 min. after treatment, the internal atmospheric conditions became constant rapidly. 2. The increase of internal $CO_2$ production was co-related with an amount of internal $O_2$ consumption, therefore, the decreasing period of internal $O_2$ consumption was equalled to the period of climacteric rise in respiration. 3. The increasing of $CO_2$ production followed evolution of $C_2$H$_4$ and this phenomenon on SAP part. was subsequent to NAP part 4. In sub-atmospheric storage, CA effect was also obtained by control of low $O_2$ and high $CO_2$ concentration.