• Title/Summary/Keyword: cleaning and sterilization effect

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Microbiological Cleaning Effect of Electrolyzed Acid Water by Containing Polysorbates (Polysorbates를 첨가한 전해산화수의 미생물학적 세정효과)

  • Jeong, Jin-Woong;Park, Kee-Jai;Jung, Sung-Won
    • Korean Journal of Food Science and Technology
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    • v.31 no.4
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    • pp.1029-1034
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    • 1999
  • To enhance the cleaning and sterilization effect of cooled electrolyzed acid water on lettuce, several kinds of polysorbates were used at various concentrations in immersion washing process. In case of the treatment containing polysorbate 20, coliform count of lettuce was reduced to about 1/40 level of that in non-treated lettuce. The treatment containing polysorbate 60 did not show a significant sterilization effect. Otherwise, the total and coliform counts of lettuce during immersion in electrolyzed acid water containing 1 ppm of polysorbate 80 was reduced to about 1/300 and 1/1,700 level of those in non-treated one. And, the changes of ORP(oxidation-reduction potential), pH and color value of lettuce in electrolyzed acid water containing 1 ppm of polysorbate 80 were not quite different during 60 min. immersion, but HCIO content decreased from 10.28 ppm to 8.51 ppm after 20 min. immersion. Also, total and coliform count of lettuce immersed in electrolyzed acid water containing 1 ppm of polysorbate 80 was lower to about 1/1,800 after 20 min. and 1/5,550 after 30 min, compared with non-treated lettuce.

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Effect of Sterilization Conditions on Microbial Reduction in Cleaning Tools (살균 조건이 세척 도구 중 미생물 저감화에 미치는 영향)

  • Im, Ji-Yu;Kim, Chae-Young;Kim, Eun-yeong;Kim, Min-jin;Kim, Jung-Beom
    • Journal of Food Hygiene and Safety
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    • v.37 no.5
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    • pp.310-316
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    • 2022
  • In this study, we compared the microbial reduction effects of drying, hot water, and microwave sterilization in scourers and dishcloths to suggest a most suitable sterilization method. Three scourer types (silver, copper, and mesh) were used, and three dishcloth types (silver, bamboo, and cotton) were used. Drying time dependent reduction in Escherichia coli was high in silver and copper scourers, but minimal bacterial reduction was obtained against Bacillus cereus in all scourers and dishcloths. In scourers, E. coli was not detected after ≥30 s of hot water sterilization at 77℃, and B. cereus was not detected after ≥60 s of hot water sterilization at 100℃. In dishcloths, E. coli was not detected after hot water sterilization at 77℃ for ≥30 s, but B. cereus was detected after hot water sterilization at 100℃ for ≥60 s. In scourers, E. coli was not detected after microwave sterilization at 700 W for 3 min, but B. cereus was detected. In dishcloths, E. coli was not detected after microwave sterilization with 700 W for ≥1 min, but B. cereus was detected in the cotton dishcloth even after sterilization for 3 min. In conclusion, the use of antimicrobial scourers (silver and copper) and dishcloths (silver and bamboo) are not sufficient to reduce the microbial contamination. The guideline provided by the Ministry of Food and Drug Safety suggesting dishcloth sterilization via hot water at 100℃ for 30 s was also found to be insufficient. Based on our research, we suggest that the most effective methods of microbial management are submerging scourers in hot water at 100℃ for ≥1 min, and sterilizing dishcloths for ≥3 min using a 700 W microwave.

A Study of the Technical Treatment within an Environmental Appetency for the Ballast Water

  • Nam, Chung-Do
    • Journal of Advanced Marine Engineering and Technology
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    • v.28 no.8
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    • pp.1313-1323
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    • 2004
  • In accordance with adoption of new convention for the control of ship's ballast water at the diplomatic conference held in London Feb, 2004, every country has to regulate the ballast water and deposit matters. When this Resolution comes into effect in 2009, all vessels engaged in international voyage must have ballast water control program, ballast water records, equipments which are suitable to the standard of exchange and performance for the ballast water. This study estimates objectively their performances, merits and demerits of the ballast water treatment technique and exchanging techniques for safe operation of ships. It is desirable to design an equipment to control the ballast water using the brush-type vacuum suction nonstop reverse cleaning system to overcome the clogging phenomenon and the direct disc filtering to maximize filtering area for the optimum process considering biological availabilities. It will be expected to protect against marine pollution and to maintain clean sea if it is secured to develop new ballast water treatment techniques. And it will also be expected to cope with the Resolution and each regulation of the developed countries from the ballast water.

Cleaning Effect of Electrolyzed Oxidizing Water by Containing Food Additives (식품첨가제를 첨가한 전해산화수의 세정효과)

  • 정승원;정진웅
    • Food Science and Preservation
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    • v.9 no.2
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    • pp.240-247
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    • 2002
  • This study, to enhance the sterilization, browning inhibition and precooling effect of electrolyzed oxidizing water(EOW) as cleaning water on food industry, was carried out to investigate the efficacy of electrolyzed oxidizing water(EOW) with 0.85% NaCl, 0.5% ethanol, polysorbate 80 of 1 ppm, 0.5% lemon juice and 0.5% citron juice. Escherichia coli KCTC 1039 with initial count of 5.63$\times$10$\^$8/ CFU/mL were reduced to <10$^1$CFU/mL after 15∼30 sec when it was treated by electrolyzed oxidizing water added with various food additives. Bacillus cereus KCTC 1012 were reduced to <10$^1$ CFU/mL after 2 minutes treatment with electrolyzed oxidizing water containing polysorbate 80 and ethanol. Iactobacillus plantarum KCTC 3108 were reduced to <10$^1$CFU/mL after 30 sec treatment with electrolyzed oxidizing water containing polysorbate 80, citron juice and lemon juice, respectively. Erwinia carotovora subsp. carotovora KCTC 2776 were reduced to <10$^1$CFU/mL after 30 sec treatment with electrolyzed oxidizing water containing polysorbate 80 and lemon juice. Browning inhibition effect was determined by comparison of polyphenol oxidase activity. Inhibition ratio of polyphenol oxidase was approximately 62∼84% in most treatments with the exception of 57% and 25% inhibition by 0.5% ascorbic acid and polysorbate 80, respectively. Sliced potato dipped in electrolyzed oxidizing water containing NaCl and citron juice for 30 minutes showed significantly low PPO activity, 64 units in treatment with NaCl and 91 units in treatment with citron juice. At the same time, changes in color value(△E) of sliced potato was below 3 in most treatments.

Design of a shoe rack for effective sterilization and deodorization of the shoes contaminated by various bacteria (오염된 신발의 효율적인 살균과 탈취를 위한 신발장의 설계)

  • Lee, Sam-Cheol;Jang, Yong-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.6
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    • pp.199-206
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    • 2016
  • The aim of this study was to establish the optimal conditions to design a shoe rack that could effectively sterilize and deodorize shoes contaminated by various bacteria. The manufacture and evaluation of a shoe rock was conducted for one year from October 2014 to September 2015. The antibacterial efficacies were evaluated using Escherichia coli and Staphylococcus aureus by JIS Z 2801:2010. The deodorization efficiency was identified by the standard method for deodorization efficiency evaluation of an indoor air cleaner established by Korea Air Cleaning Association, SPS-KACA002-132:2006. The results showed that antibacterial evaluation showed more than 99.9% of the sterilization effects on Escherichia coli and Staphylococcus aureus after exposure to ultraviolet (UV) light for 30 seconds. The average deodorization efficiency for the test gases was 42.5%. The temperature in the shoes and in the shoe rack was lower than $40^{\circ}C$ and $25^{\circ}C$, respectively, when the UV lamp was turned on for 5 minutes and off for 25 minutes repeatedly. This was controlled by turning the UV lamp on and off to maintain the appropriate temperature in the shoes. In conclusion, the real deodorization efficiency is expected to be higher than the measured value because the internal volume of the shoe rack was smaller than the test chamber used to measure the deodorization efficiency. The deodorization effect could be increased further by the sterilization of bacteria causing bad odors in shoes. Further studies will be needed to determine the temperature deviations within the shoe rack to provide constant conditions.

Cleaning and Storage Effect of Electrolyzed Water Manufactured by Various Electrolytic Diaphragm (격막 방식에 따라 제조한 전해수의 세척 및 보관 효과)

  • 김명호;정진웅;조영제
    • Food Science and Preservation
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    • v.11 no.2
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    • pp.160-169
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    • 2004
  • This study was carried out to investigate the efficacy of electrolyzed water manufactured with or without diaphragm on sterilization and preservation of cut-celery and shelled raw oyster. In cut-celery, total viable cell count and coliform group in the treatment of electrolyzed water were decreased to about 1/200∼1/1,000 level and about 1/100 level comparing non-treated ones. But moisture content, pH, hardness, vitamin C and residual chlorine content were showed a little difference among treatments up to 10 days at 10$^{\circ}C$. L and a color values were gradually increased in all treatments, and color differences($\Delta$E) were remarkable between treatment and untreatment sample. In overall acceptability, cut-celery treated with electrolyzed water showed somewhat higher score than that of other ones treated with tap water and 100 ppm NaClO solution until 5 days of storage. After 48 hours of storage, it was showed that VBN, total viable cell count and coliform count of shelled raw oyster treated with electrolyzed alkali water produced by non-diaphragm system are lower by about 3 mg%, 1∼2 log cycle and 2 log cycle respectively than that of ones treated with sea water. Total viable cell count of shelled raw oyster just after treatment was lower by about 1 log cycle than that of ones treated with sea water, and any significant increment was not found after 24∼48 hours of storage.

Comparison of Quality Characteristics of Sesame leaf Cleaned with Various Electrolyzed Water during Storage (다양한 전기분해수 세정처리에 따른 깻잎의 저장중 품질특성 비교)

  • Jeong Jin-Woong;Kim Jong-Hoon;Kwon Kee-Hyun
    • Food Science and Preservation
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    • v.12 no.6
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    • pp.558-564
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    • 2005
  • This study was carried out to investigate the cleaning effect of sesame leaf, the sterilization effect and physicochemical properties, treated with various electrolyzed water. Initial physicochemical properties could be kept more than 1 month in electrolyzed oxidizing water(EW-1) of diaphragm type and 15 days in electrolyzed water(EW-2 and EW-3) of non-diaphragm system, there was no significant difference by storage temperature. 4 kinds of microorganism (initial total counts, $10^7\~10^9$ CFU/mL) were sterilized within $0.5\~1$ minutes by electrolyzed water. In fresh sesame leaves, total viable cell count and coliform group in the treatment of electolyzed water were decreased to about $2\~3$ log scale comparing non-treated ones. Especially Bacillus cereus was not detected until 13th day when treated with EW-l. Decaying ratio of sesame leaf appears on day 6 of storage in the untreated but the treatments of electrolyzed water has no sign until day 10 of storage. Change in color difference(${\Delta}E$) during storage was observed the treatments of electrolyzed low-alkaline water(EW-2) and electrolyzed neutral water(EW-3) were very desirable at the level $1\~2$ after day 13 of storage comparative to the untreated Change of Chlorophyll content was biggest decreased to 6.8 $mg\%$ on the untreated and decreased least to 8.35 $mg\%$ on EW-3 treated group on 13th day from initial value of $9.0\~10.3\;mg\%$ The overall sensory evaluation appeared most acceptable in the treatments of EW-2 and EW-3.

Analysis of surface form change after performing prophylaxis procedure on implant surface using various oral hygiene instruments (다양한 구강위생기구를 이용하여 임플란트 표면의 Prophylaxis 시행시 표면형태의 변화분석)

  • Lee, Sun-Goo;Lim, Sung-Bin;Chung, Chin-Hyung;Kwon, Sang-Ho
    • Journal of Periodontal and Implant Science
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    • v.34 no.1
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    • pp.1-17
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    • 2004
  • It is improtant that performing prophylaxis procedure on an infected implant surface in order to treat peri-implantitis should not change the surface roughness and composition, so that the surface can be recovered to almost same condition as initial implant surface. This thesis, therefore, studied an effect of various oral hygiene instrument on implant surface. A surface roughness measurement instrument and an infection electron microscope were used to observe a change on surface. The purpose of this study was to obtain a clinical guidelines during implant care and peri-implantitis treatment. The result were as follows 1. Ra values (surface roughness value) at experimental group 1, group 2, and group 5 were increased significantly as compared with comparison group(p<0.05). 2. When compared experimental group 1 with each experimental groups at which prohylaxis procedure was performed, mean values of Ra at experimental group 2, group 3, group 6, and group 7 were decreased significantly(p<0.05). 3. Mean value of Ra was lowest at experimental group 2, and highest at experimental group 2, and highest at experimental group 5. 4. Analysis of SEM showed that was significant surface change at experimental group 2, group 3, group 4, group 5, and group 6 as compared with comparison group(X1000). 5. Analysis fo EDX showed that a quantity of Ti on surface for experimental group 6 was very similar to that for comparison group. In conclusion, air-powder abrasive and citric acid, plastic instrument are safe methods to use for performing prophylaxis procedure on implant care or for cleaning and sterilization process on treatment of peri-implantitis, based on the result that those method did not affect implant surface roughness and Ti composition.