• Proceedings of the KIEE Conference
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• 1997.07f
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• pp.2016-2019
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• 1997

To solve the low efficiency problem of low-voltage power supplies, it has been studied to replace the schottky barrier diode with the MOSFET synchronous rectifier. In this paper, Phase Shift-Controlled Clamp Mode Zero Voltage Switching-Multi Resonant Converter with Synchronous Rectifier (PSC CM ZVS-MRC with SR) is presented to achieve high efficiency in low-voltage power supplies. The characteristics analysis of synchronous rectifier is established by using the MOSFET equivalent circuit and efficiency comparison is established between the Synchronous Rectifier and the schottky barrier diode. To verify the validity of the analysis, 33W(3.3V, 10A) PSC CM ZVS-MRC with self-driven synchronous rectifier at switching frequency of 1MHz is designed and tested. And it is confirmed that the experimental results are well consistent with the theoretical results. The maximum efficiency of the converter is 83.4% at full load, which is 3.3% higher than conventional schottky diode rectification.

• Journal of Power Electronics
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• v.16 no.2
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• pp.778-785
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• 2016

An excess carrier lifetime extraction method is derived for physics-based insulated gate bipolar transistor (IGBT) models with consideration of the latest development in IGBT modeling. On the basis of the 2D mixed-mode Sentaurus simulation, the clamp turn-off test is simulated to obtain the tail current. The proposed excess carrier lifetime extraction method is then performed using the simulated data. The comparison between the extracted results and actual lifetime directly obtained from the numerical device model precisely demonstrates the accuracy of the proposed method.

• Proceedings of the KIEE Conference
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• 2006.07b
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• pp.1057-1058
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• 2006

In this paper, the proposed power supply is based on a modified version of the zero-voltage switching(ZVS) full-bridge phase-shift DC-DC converter, which incorporates commutation auxiliary inductors to provide ZVS for the entire load range as well as a commutation aid circuit to clamp the output diode voltage. The control strategy is based on two control loops operating in cascade mode. The complete operating principles and simulation results in presented.

• Proceedings of the KIEE Conference
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• 1995.07a
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• pp.191-194
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• 1995

소프트 스위칭 능동 클램프 회로와 위상 변위 제어회로를 이용하여 위상 변위 제어 클램프 모드 포워드 다중진공형 컨버터를 제안하였다. 제안된 컨버터는 일정한 스위칭 주파수에서 동작하므로 설계가 용이하고 주 스위치의 전압 스트레스를 입력 전압의 2-3배로 줄일 수 있다. 컴퓨터 시뮬레이션을 통해 제안된 회로의 이론적인 동작 파형을 확인하였으며 타당성을 입증하기 위하여 1MHz, 5V, 50W 위상 변위 제어 클램프 모드 포워드 다중공진형 컨버터를 제작하여 실험한 결과 주스위치의 최대 전압 스트레스는 입력전압의 3배로 저감하였고, 부하 전류가 증가함에 따라 주스위치에 걸리는 전압 스트레스와 전류 스트레스가 감소하며, 최고 84.2%의 효율을 얻었다.

• Proceedings of the Korean Biophysical Society Conference
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• 1997.07a
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• pp.26-26
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• 1997

In the present study, we recorded CCh-activated nonselective cation (NSC) current in guinea-pig gastric smooth muscle cells and investigated the characteristics of the current. In whole-cell voltage-clamp mode, CCh activated NSC current. The same NSC current could be activated by internal dialysis of GTP${\gamma}$S.(omitted)

• Molecules and Cells
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• v.27 no.5
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• pp.525-531
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• 2009

We studied the effect of carbachol on pacemaker currents in cultured interstitial cells of Cajal (ICC) from the mouse small intestine by muscarinic stimulation using a whole cell patch clamp technique and $Ca^{2+}$-imaging. ICC generated periodic pacemaker potentials in the current-clamp mode and generated spontaneous inward pacemaker currents at a holding potential of -70 mV. Exposure to carbachol depolarized the membrane and produced tonic inward pacemaker currents with a decrease in the frequency and amplitude of the pacemaker currents. The effects of carbachol were blocked by 1-dimethyl-4-diphenylacetoxypiperidinium, a muscarinic $M_3$ receptor antagonist, but not by methotramine, a muscarinic $M_2$ receptor antagonist. Intracellular $GDP-{\beta}-S$ suppressed the carbachol-induced effects. Carbachol-induced effects were blocked by external $Na^+$-free solution and by flufenamic acid, a non-selective cation channel blocker, and in the presence of thapsigargin, a $Ca^{2+}$-ATPase inhibitor in the endoplasmic reticulum. However, carbachol still produced tonic inward pacemaker currents with the removal of external $Ca^{2+}$. In recording of intracellular $Ca^{2+}$ concentrations using fluo 3-AM dye, carbachol increased intracellular $Ca^{2+}$ concentrations with increasing of $Ca^{2+}$ oscillations. These results suggest that carbachol modulates the pacemaker activity of ICC through the activation of non-selective cation channels via muscarinic $M_3$ receptors by a G-protein dependent intracellular $Ca^{2+}$ release mechanism.

• Journal of Pharmacopuncture
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• v.16 no.1
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• pp.43-49
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• 2013

Objective: Pyungwi-san (PWS) plays a role in a number of physiologic and pharmacologic functions in many organs. Interstitial cells of Cajal (ICCs) are pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We aimed to investigate the beneficial effects of PWS in mouse small-intestinal ICCs. Methods: Enzymatic digestion was used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane potentials from the cultured ICCs. Results: ICCs generated pacemaker potentials in the GI tract. PWS produced membrane depolarization in the current clamp mode. Pretreatment with a $Ca^{2+}$-free solution and a thapsigargin, a $Ca^{2+}$-ATPase, inhibitor in the endoplasmic reticulum, eliminated the generation of pacemaker potentials. However, only when the thapsigargin was applied in a bath solution, the membrane depolarization was not produced by PWS. Furthermore, the membrane depolarizations due to PWS were inhibited not by U-73122, an active phospholipase C inhibitor, but by chelerythrine and calphostin C, protein kinase C inhibitors. Conclusions: These results suggest that PWS might affect GI motility by modulating the pacemaker activity in the ICCs.

• Journal of Korean Neurosurgical Society
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• v.38 no.5
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• pp.375-379
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• 2005

Objective : Papaverine has been used in treating vasospasm following subarachnoid hemorrhage[SAH]. However, its action mechanism for cerebral vascular relaxation is not clear. Potassium channels are closely related to the contraction and relaxation of cerebral smooth muscle. Therefore, to identify the role of potassium and calcium channels in papaverine-induced vascular relaxation, we examine the effect of papaverine on potassium channels in freshly isolated smooth muscle cells from rat basilar artery. Methods : The isolation of rat basilar smooth muscle cells was performed by special techniques. The whole cell currents were recorded by whole cell patch clamp technique in freshly isolated smooth muscle cells from rat basilar artery. Papaverine was added to the bath solution. Results : Papaverine of $100{\mu}M$ into bath solution increased the amplitude of the outward $K^+$ current which was completely blocked by BKCa[large conductance calcium dependent potassium channels]blocker, IBX[iberiotoxin], and calcium chealator, BAPTA[l,2-bis[o-aminophenoxy]ethane-N,N,N',N'-tetraacetic acid], in whole cell mode. Conclusion : These results strongly suggest that potassium channels may play roles in papaverine-induced vascular relaxation in rat basilar artery.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.2
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• pp.149-156
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• 2013

Interstitial cells of Cajal (ICCs) are the pacemaker cells in the gastrointestinal tract, and histamine is known to regulate neuronal activity, control vascular tone, alter endothelial permeability, and modulate gastric acid secretion. However, the action mechanisms of histamine in mouse small intestinal ICCs have not been previously investigated, and thus, in the present study, we investigated the effects of histamine on mouse small intestinal ICCs, and sought to identify the receptors involved. Enzymatic digestions were used to dissociate ICCs from small intestines, and the whole-cell patch-clamp configuration was used to record potentials (in current clamp mode) from cultured ICCs. Histamine was found to depolarize resting membrane potentials concentration dependently, and whereas 2-PEA (a selective H1 receptor agonist) induced membrane depolarizations, Dimaprit (a selective H2-agonist), R-alpha-methylhistamine (R-alpha-MeHa; a selective H3-agonist), and 4-methylhistamine (4-MH; a selective H4-agonist) did not. Pretreatment with $Ca^{2+}$-free solution or thapsigargin (a $Ca^{2+}$-ATPase inhibitor in endoplasmic reticulum) abolished the generation of pacemaker potentials and suppressed histamine-induced membrane depolarization. Furthermore, treatments with U-73122 (a phospholipase C inhibitor) or 5-fluoro-2-indolyl des-chlorohalopemide (FIPI; a phospholipase D inhibitor) blocked histamine-induced membrane depolarizations in ICCs. On the other hand, KT5720 (a protein kinase A inhibitor) did not block histamine-induced membrane depolarization. These results suggest that histamine modulates pacemaker potentials through H1 receptor-mediated pathways via external $Ca^{2+}$ influx and $Ca^{2+}$ release from internal stores in a PLC and PLD dependent manner.

• The Transactions of the Korean Institute of Power Electronics
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• v.14 no.6
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• pp.496-503
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• 2009

In this paper, a new three-phase interleaved isolated high efficiency boost dc-dc converter with active clamp is proposed. The converter is capable of increased power transfer due to its three-phase power configuration, and it reduces the rms current per phase, thus reducing conduction losses. Further, interleaved operation of three-phase boost converter reduces overall ripple current, which is imposed into fuel cells and realizes smaller sized filter components, increasing effective operating frequency and leading to higher power density. Each output current of three-phase boost converter is combined by the three-phase transformer and flows in the continuous conduction mode by the proposed three-phase PWM strategy. An efficiency of above 96% is mainly achieved by reducing conduction losses and switching losses are reduced by the action of active clamp branches, as well. The proposed converter and three-phase PWM strategy are analyzed, simulated and implemented in hardware. Experimental results are obtained on a 500 W prototype unit, with all of the design verified and analyzed.